Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 89, Issue 4, Pages 1019-1028Publisher
SPRINGER
DOI: 10.1007/s00253-010-2960-1
Keywords
Aglycon protopanaxadiol; Compound K; beta-glycosidase; Pyrococcus furiosus
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Funding
- Seoul RBD Program [PA090939]
- Korea government [2010-0019306]
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The production of compound K and aglycon protopanaxadiol (APPD) from ginsenoside Rd and ginseng root extract was performed using a recombinant beta-glycosidase from Pyrococcus furiosus. The activity for Rd was optimal at pH 5.5 and 95A degrees C with a half-life of 68 h at 95A degrees C. beta-Glycosidase converted Rb-1, Rb-2, Rc, and Rd to APPD via compound K. With increases in the enzyme activity, the productivities of compound K and APPD increased. The substrate concentration was optimal at 4.0 mM Rd or 10% (w/v) ginseng root extract; 4 mM of Rd was converted to 3.3 mM compound K with a yield of 82.5% (mol/mol) and a productivity of 2,010 mg l(-1) h(-1) at 1 h and was hydrolyzed completely to APPD with 364 mg l(-1) h(-1) after 5 h. Rb-1, Rb-2, Rc, and Rd at 3.9 mM in 10% ginseng root extract were converted to 3.1 mM compound K with 79.5% and 1,610 mg l(-1) h(-1) at 1.2 h and were hydrolyzed completely to APPD with 300 mg l(-1) h(-1) after 6 h. The concentrations and productivities of compound K and APPD in the present study are the highest ever reported.
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