Article
Biochemistry & Molecular Biology
Minjie Gao, Guoshuai Yang, Feifei Li, Zichao Wang, Xiuyu Hu, Yun Jiang, Jiajun Yan, Zhitao Li, Xiaobei Zhan
Summary: This study involved the production of curdlan oligosaccharides by expressing endo-11-1,3-glucanase in Pichia pastoris and constructing a co-culture system. The optimized conditions in a 7-L bioreactor led to a maximum yield of 18.77 g/L of curdlan oligosaccharides with 3-10 degrees of polymerization, presenting a novel strategy for oligosaccharide production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Min-Jie Gao, Jia-Jun Yan, Yue Zhao, Li Zhu, Guo-Shuai Yang, Xiao-Bei Zhan
Summary: In this study, the endo-beta-1,3-glucanase gene from Trichoderma harzianum was successfully expressed in Pichia pastoris KM71, resulting in a high-yield endo-beta-1,3-glucanase strain. The recombinant enzyme exhibited optimal activity at pH 5.5 and 50 degrees C, with efficient hydrolysis capabilities towards scleroglucan and schizophyllan.
PROCESS BIOCHEMISTRY
(2021)
Article
Green & Sustainable Science & Technology
Minjie Gao, Feiyang Duan, Liping Liu, Xiuyu Hu, Li Zhu, Yun Jiang, Xiaobei Zhan
Summary: Utilizing waste liquid containing miscellaneous waste carbohydrates (MWC) from the high-fructose syrup (HFS) production industry in China as an alternative carbon source for Pichia pastoris fermentation, the study successfully optimized the fermentation processes and achieved high production of endo-beta-1,3-glucanase. This cleaner production approach significantly reduced environmental pollution and production costs in both HFS and P. pastoris fermentation industries.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Agricultural Engineering
Minjie Gao, Ying Xu, Guoshuai Yang, Shuxia Jin, Xiuyu Hu, Yun Jiang, Li Zhu, Zhitao Li, Xiaobei Zhan
Summary: The successful implementation of heterologous expression with error-prone PCR technology in Pichia pastoris led to the mutant strain 7 N12 with efficient specific activities for degrading curdlan and scleroglucan. Coupled fermentation of Pichia pastoris and Sclerotium rolfsii simplified the enzymatic 8-oligoglucoside preparation procedures, showing potential for industrial production of functional oligosaccharides.
BIORESOURCE TECHNOLOGY
(2021)
Article
Food Science & Technology
Marinela Sokarda Slavic, Milan Kojic, Aleksandra Margetic, Marina Ristovic, Marija Pavlovic, Stefan Nikolic, Zoran Vujcic
Summary: The study focused on the impact of the enzyme endo-beta-1,3-1,4-glucanase from Bacillus subtilis 168 on improving the nutritional and bioactive properties of barley beta-glucan. A new procedure for isolating pure beta-glucan from barley flour was developed, enabling it to be free from starch contamination. The cloned enzyme showed high efficiency in degrading beta-glucan and increasing its antioxidant capacities, suggesting potential applications in functional foods.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tomoya Ota, Wataru Saburi, Takayoshi Tagami, Jian Yu, Shiro Komba, Linda Elizabeth Jewell, Tom Hsiang, Ryozo Imai, Min Yao, Haruhide Mori
Summary: The mechanism of endo-type action on laminarin by GH55 endo-beta-1,3-glucanase MnLam55A was investigated, revealing its preference for cleaving the nonreducing terminal beta 1-3-linkage of the laminar-ioligosaccharide moiety. The crystal structure of MnLam55A showed similarities to exo-beta-1,3-glucosidases, but possessed an extended substrate-binding cleft.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Zhen Qin, Sainan Yu, Kemin Zhang, Xiasen Wei, Junjie Li, Zheyi Zhang, Sibao Wan, Haiyan Gao
Summary: In this study, a β-1,3-glucanase named BsGlc157A was identified from Bacteroides sp. M27. It exhibited optimal catalytic activity at pH 6.0 and 40 degrees C. BsGlc157A adopted a classic (beta/alpha)(8) TIM-barrel structure and two catalytic residues were confirmed. Moreover, BsGlc157A showed inhibitory effects on the hyphal growth of fruit pathogenic fungi, highlighting its potential as a biocontrol agent.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Kaili Wang, Siyu Huai, Zhuqing Tan, Guillaume Legrand Ngolong Ngea, Esa Abiso Godana, Jun Shi, Qiya Yang, Xiaoyun Zhang, Lina Zhao, Hongyin Zhang
Summary: This study successfully expressed and purified an endo-beta-1,3-glucanase PeBgl1 from Penicillium expansum, and analyzed its characteristics. The PeBgl1 protein showed good enzyme activity at 50-60 degrees C and at pH 5.0-9.0, and it was not metal dependent, making it potentially robust for storage and transportation.
Article
Plant Sciences
Lavanya Mendu, Gayani Jalathge, Kamalpreet Kaur Dhillon, Nagendra Pratap Singh, Vimal Kumar Balasubramanian, Rebecca Fewou, Dennis C. Gitz, Junping Chen, Zhanguo Xin, Venugopal Mendu
Summary: The present study identified and characterized the function of the endo-1,4-beta-glucanase gene in sorghum, laying the foundation for future research on cell wall biosynthesis and biofuel production.
Article
Biochemistry & Molecular Biology
Zhengxue Li, Chengtuo Niu, Xiaohong Yang, Feiyun Zheng, Chunfeng Liu, Jinjing Wang, Qi Li
Summary: In this study, a mesophilic beta-glucanase from Bacillus terquilensis CGX 5-1 was engineered to improve its stability at acidic environment and catalytic property. The Q1E/I133L variant showed better acidic stability and higher catalytic activity, with potential applications in industries. The improved acidic resistance ability of the variant may be attributed to more surface negative charge, decreased exposure degree of residue No.1, shifted side chain direction of residue No.133, and lower total and folding free energy.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Lu Bai, Jonghoon Kim, Kwang-Hee Son, Dong-Ha Shin, Bon-Hwan Ku, Do Young Kim, Ho-Yong Park
Summary: Endo-beta-1,3-glucanase from Cellulosimicrobium funkei HY-13 was found to have high activity in degrading laminarin and displayed strong growth inhibition against Candida albicans. Deleting the RICIN domain in the enzyme resulted in a significant decrease in its ability to degrade beta-1,3-D-glucans. The findings suggest that this enzyme could be potentially utilized as a supplementary agent in the food and pharmaceutical industries due to its superior anti-fungal activity.
Article
Biotechnology & Applied Microbiology
Jinyang Li, Xiao Wang, Jiahuan Zou, Kun Yang, Xiaolu Wang, Yuan Wang, Honglian Zhang, Huoqing Huang, Xiaoyun Su, Bin Yao, Huiying Luo, Xing Qin
Summary: This study applied a CRISPR/Cas9 system to the acidophilic fungus Acidomyces richmondensis MEY-1 and identified the determinants of Cu resistance. The Cu-binding transcription factor ArAceA and the Cu-transporting P-type ATPase ArYgA were found to play roles in Cu tolerance and pigmentation, respectively. These findings provide insights into the molecular basis of Cu tolerance in A. richmondensis MEY-1 and highlight the potential of the CRISPR/Cas9 system for studying adaptability mechanisms in acidophilic fungi.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Environmental Sciences
Zi-Qian Zha, Shuai You, Yang-Hao Hu, Fang Zhang, Yi-Wen Chen, Jun Wang
Summary: N-glycosylation can alter the properties of enzymes, such as increasing thermostability and catalytic efficiency. This study focused on the modification and utilization of GH16 family glucanases, showing that N-glycosylation can enhance their stability and efficiency.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Huabiao Miao, Yuanhao Zhao, Yu Ma, Nanyu Han, Yuanyuan Zhe, Xianghua Tang, Zunxi Huang
Summary: In this study, the thermostability of CMCase-I enzyme was improved by engineering and inserting the C2 submodule. The resulting C2-CMCase-I chimera showed significantly enhanced stability and the formation of a new beta-sheet structure. This chimera has potential for industrial applications.
PROCESS BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Shumao Chai, Xueliang Zhang, Yuejiao Gao, Lishuang Nie, Chenchen Xiang, Mingming Cao, Shaochen Wang, Zhiyang Feng
Summary: A novel endo-/β-1,4-glucanase ZFYN1413-87 with strong enzyme activity was identified and studied in this research, showing 52% retained activity even after treatment in pH 3.0 buffer for 2 hours, representing a unique example in the GH6 family.
CARBOHYDRATE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jun Yang, Sunny Sharma, Peter Koetter, Karl-Dieter Entian
NUCLEIC ACIDS RESEARCH
(2015)
Article
Genetics & Heredity
Sunny Sharma, Jun Yang, Rob van Nues, Peter Watzinger, Peter Koetter, Denis L. J. Lafontaine, Sander Granneman, Karl-Dieter Entian
Article
Biotechnology & Applied Microbiology
Huiying Luo, Yaru Wang, Hui Wang, Jun Yang, Yuhui Yang, Huoqing Huang, Peilong Yang, Yingguo Bai, Pengjun Shi, Yunliu Fan, Bin Yao
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2009)
Article
Biotechnology & Applied Microbiology
Huiying Luo, Jun Yang, Jiang Li, Pengjun Shi, Huoqing Huang, Yingguo Bai, Yunliu Fan, Bin Yao
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2010)
Article
Biotechnology & Applied Microbiology
Huiying Luo, Yaru Wang, Jiang Li, Hui Wang, Jun Yang, Yuhui Yang, Huoqing Huang, Yunliu Fan, Bin Yao
ENZYME AND MICROBIAL TECHNOLOGY
(2009)
Article
Biochemistry & Molecular Biology
Huiying Luo, Jiang Li, Jun Yang, Hui Wang, Yuhui Yang, Huoqing Huang, Pengjun Shi, Tiezheng Yuan, Yunliu Fan, Bin Yao
Article
Biochemistry & Molecular Biology
Sunny Sharma, Jun Yang, Peter Watzinger, Peter Koetter, Karl-Dieter Entian
NUCLEIC ACIDS RESEARCH
(2013)
Article
Biochemistry & Molecular Biology
Sunny Sharma, Jun Yang, Simon Duettmann, Peter Watzinger, Peter Koetter, Karl-Dieter Entian
NUCLEIC ACIDS RESEARCH
(2014)
Article
Multidisciplinary Sciences
Jun Yang, Sunny Sharma, Peter Watzinger, Johannes David Hartmann, Peter Koetter, Karl-Dieter Entian
Article
Biochemistry & Molecular Biology
Jun Yang, Huiying Luo, Jiang Li, Kun Wang, HaiPing Cheng, Yingguo Bai, Tiezheng Yuan, Yunliu Fan, Bin Yao
PROCESS BIOCHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Britta Meyer, Carina Immer, Steffen Kaiser, Sunny Sharma, Jun Yang, Peter Watzinger, Lena Weiss, Annika Kotter, Mark Helm, Hans-Michael Seitz, Peter Koetter, Stefanie Kellner, Karl-Dieter Entian, Jens Woehnert
NUCLEIC ACIDS RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Sunny Sharma, Ewa Grudzien-Nogalska, Keith Hamilton, Xinfu Jiao, Jun Yang, Liang Tong, Megerditch Kiledjian
NUCLEIC ACIDS RESEARCH
(2020)
Article
Multidisciplinary Sciences
Xiaowen Wang, Hong Zhang, Russell Sapio, Jun Yang, Justin Wong, Xin Zhang, Jessie Y. Guo, Sharon Pine, Holly Van Remmen, Hong Li, Eileen White, Chen Liu, Megerditch Kiledjian, Dimitri G. Pestov, X. F. Steven Zheng
Summary: The study reveals a crucial role of nuclear SOD1 in ribosome biogenesis and proliferation in KRAS-driven lung cancer.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Sunny Sharma, Jun Yang, Ewa Grudzien-Nogalska, Jessica Shivas, Kelvin Y. Kwan, Megerditch Kiledjian
Summary: The cytoplasmic protein Xrn1 is found to be capable of degrading RNAs with an unprotected 5' monophosphate end as well as RNAs harboring noncanonical NAD caps. The deNADding activity of Xrn1 is crucial for normal growth and regulation of mitochondrial NAD-capped RNA levels and intramitochondrial NAD levels.
NATURE COMMUNICATIONS
(2022)