Article
Food Science & Technology
Bingnan Liu, Xinyu Sun, Yue Liu, Mengmeng Yang, Liang Wang, Ying Li, Jihui Wang
Summary: This study successfully increased the supply of reducing power NADPH and improved the production of L-methionine through genetic modification and heterologous expression. The engineered strain YM6 with high NADPH supply and high L-methionine production was constructed, and metabolic flux analysis confirmed the increase in its synthesis.
Review
Biotechnology & Applied Microbiology
Min Duan, Shuo Chen, Xinli Liu, Jianhang Liu, Deqiang Zhu
Summary: This article provides a comprehensive review of the function and application of l-threonine, the pathway of C. glutamicum to synthesize l-threonine, and the use of metabolic engineering technology to improve its production.
FERMENTATION-BASEL
(2023)
Article
Microbiology
Hedan Li, Daqing Xu, Dezhi Zhang, Xin Tan, Danyang Huang, Wenjian Ma, Guihong Zhao, Ying Li, Ziwei Liu, Yang Wang, Xiaoqing Hu, Xiaoyuan Wang
Summary: In this study, the researchers investigated the effect of biosynthesis of trehalose dicorynomycolates on L-isoleucine production in C. glutamicum by deleting several genes. They found that the production of L-isoleucine was significantly improved in the triple deletion mutant ?treSYA. Further deletion of mytA or mytB in ?treSYA could further enhance the production, but deletion of both mytA and mytB significantly decreased the production. Ultimately, they constructed the final L-isoleucine producing strain WL001, which showed a 36.1% increase in L-isoleucine production compared to the control strain WM001 after 72 hours of shake flask cultivation.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Ning Li, Xiaoyu Shan, Jingwen Zhou, Shiqin Yu
Summary: This study successfully identified and regulated key genes involved in OAH biosynthesis, leading to increased OAH production. It demonstrated that the identification and regulation of key genes are crucial for industrial production of OAH in Corynebacterium glutamicum.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Zenon Konteatis, Jeremy Travins, Stefan Gross, Katya Marjon, Amelia Barnett, Everton Mandley, Brandon Nicolay, Raj Nagaraja, Yue Chen, Yabo Sun, Zhixiao Liu, Jie Yu, Zhixiong Ye, Fan Jiang, Wentao Wei, Cheng Fang, Yi Gao, Peter Kalev, Marc L. Hyer, Byron DeLaBarre, Lei Jin, Anil K. Padyana, Lenny Dang, Joshua Murtie, Scott A. Biller, Zhihua Sui, Kevin M. Marks
Summary: This study reports the discovery of highly potent, selective MAT2A inhibitors that overcome previous challenges in targeting MAT2A. Through fragment screening and structure-guided design, these inhibitors significantly reduce SAM levels in cancer cells and selectively block proliferation of MTAP-null cells.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Claudia De Fusco, Marianne Schimpl, Ulf Borjesson, Tony Cheung, Iain Collie, Laura Evans, Priyanka Narasimhan, Christopher Stubbs, Mercedes Vazquez-Chantada, David J. Wagner, Michael Grondine, Matthew G. Sanders, Sharon Tentarelli, Elizabeth Underwood, Argyrides Argyrou, James M. Smith, James T. Lynch, Elisabetta Chiarparin, Graeme Robb, Sharan K. Bagal, James S. Scott
Summary: MAT2a is an enzyme that synthesizes the essential metabolite SAM, making it an attractive target for treating MTAP-deleted cancers sensitive to its inhibition. Through structure-guided design and systematic SAR exploration, a potent series of MAT2a inhibitors has been developed, demonstrating promising antitumor response in in vivo studies.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Biotechnology & Applied Microbiology
Shengzhu Yu, Bo Zheng, Zhenya Chen, Yi-Xin Huo
Summary: Branched chain amino acids have been synthesized through metabolic engineering in Corynebacterium glutamicum. Further research should focus on the feedback inhibition and transcription attenuation mechanisms of key enzymes, as well as the development of enzymes with substrate specificity for the production of individual BCAAs. The strategies used in constructing BCAAs-producing strains provide valuable guidance for the biosynthesis of other high value-added compounds.
MICROBIAL CELL FACTORIES
(2021)
Review
Biochemistry & Molecular Biology
Joanna Perla-Kajan, Hieronim Jakubowski
Summary: Dysregulation of one-carbon metabolism is associated with various diseases and plays a crucial role in COVID-19. The metabolism of cells infected by the SARS-CoV-2 virus is altered, affecting vitamin B-12 and glutathione metabolism, which may be related to the symptoms and disease mechanisms of COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Hao Zhang, Zhilin Ouyang, Nannan Zhao, Shuangyan Han, Suiping Zheng
Summary: In this study, the AmtR regulon in C. glutamicum ATCC 14067 was identified using ChIP-seq, revealing new operons related to creatine and creatinine degradation. The negative regulation of transcription and expression of these genes by AmtR was confirmed. Additionally, it was found that C. glutamicum ATCC 14067 can utilize creatine or creatinine as a sole nitrogen source.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ying Luo, Anping Su, Jinshui Yang, Qijun Yu, Entao Wang, Hongli Yuan
Summary: This study demonstrated an economic way to produce 5-aminolevulinic acid (ALA) using a microbial platform. Through genetic engineering modifications and improving cell permeability, the production of ALA was significantly increased. The foliar spray of ALA produced by B. cereus PT1-hemA-Delta tagU strain improved salt tolerance and enhanced the growth of cucumber plants.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Microbiology
Sheng Yin, Dongmei Yang, Yiying Zhu, Baozhu Huang
Summary: The study reveals that amino acid metabolism can regulate the biosynthesis of Monascus pigments (MPs), and methionine and S-adenosylmethionine (SAM) play different roles in the regulation of MPs biosynthesis in Monascus purpureus RP2. The expression of SAM synthetase leads to an increased expression of transcriptional regulatory proteins of the MPs biosynthesis gene cluster, while the addition of SAM results in increased expression of MPs biosynthesis activators and the global regulator LaeA. These findings provide a new perspective for understanding the regulation of MPs biosynthesis in M. purpureus.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Young Jin Ko, Minhye Kim, Seung Kyou You, Sang Kyu Shin, Joonhee Chang, Hae Jin Choi, Wu-Young Jeong, Myeong-Eun Lee, Dong-Hyeok Hwang, Sung Ok Han
Summary: The study demonstrated the development of engineered Corynebacterium glutamicum capable of high-yield production of heme, with a maximum titer of 309.18 mg/L, including secreted heme of 242.95 mg/L, which are the highest values reported to date. Various strategies including system metabolic engineering and modification of membrane surface were utilized to enhance heme production, making C. glutamicum a potential cell factory for animal-free heme production.
METABOLIC ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Ning Liu, Ting-Ting Zhang, Zhi-Ming Rao, Wei-Guo Zhang, Jian-Zhong Xu
Summary: In Corynebacterium glutamicum, knocking out the nitrogen source regulatory protein AmtR increased L-lysine production, while upregulating the dehydrogenase pathway through genetic manipulation further enhanced L-lysine biosynthesis. The final engineered strain achieved a significantly higher L-lysine titer and maximum specific rate compared to the original strain, indicating the potential of the dehydrogenase pathway in promoting L-lysine production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Yu Li, Wangdan Xiong, Feng He, Tianxiong Qi, Zhen Sun, Yuchen Liu, Shiqie Bai, Honglun Wang, Zhenying Wu, Chunxiang Fu
Summary: The down-regulation of PvSAMS in switchgrass can reduce the contents of SAM, G-lignins, and S-lignins, leading to the accumulation of methionine and glucoside derivatives of caffeoyl alcohol, and resulting in brownish stems. Transcriptomic analysis shows that most sulfur deficiency-responsive genes are differentially expressed in the transgenic switchgrass, leading to an increase in total sulfur content.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biotechnology & Applied Microbiology
Melanie Mindt, Lenny Ferrer, Dirk Bosch, Katarina Cankar, Volker F. Wendisch
Summary: Indole has gained increasing interest in the flavor and fragrance industry due to its distinct floral odor. However, the current production methods are non-sustainable and unprofitable. To offer an alternative approach, biosynthesis of indole has been studied, including glucose-based production using genetically-engineered strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Takahisa Kogure, Masayuki Inui
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Yota Tsuge, Naoto Kato, Shogo Yamamoto, Masako Suda, Masayuki Inui
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2019)
Article
Biotechnology & Applied Microbiology
Tetsu Shimizu, Haruhiko Teramoto, Masayuki Inui
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Yota Tsuge, Naoto Kato, Shogo Yamamoto, Masako Suda, Toru Jojima, Masayuki Inui
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Editorial Material
Biochemical Research Methods
Sung Ok Han, Masayuki Inui, Yong-Su Jin
BIOTECHNOLOGY JOURNAL
(2019)
Article
Biotechnology & Applied Microbiology
Shinichi Oide, Yuya Tanaka, Akira Watanabe, Masayuki Inui
ENZYME AND MICROBIAL TECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Tetsu Shimizu, Haruhiko Teramoto, Masayuki Inui
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Satoshi Hasegawa, Toru Jojima, Masako Suda, Masayuki Inui
METABOLIC ENGINEERING
(2020)
Article
Biotechnology & Applied Microbiology
Hiroto Uchikura, Koichi Toyoda, Hiroki Matsuzawa, Hikaru Mizuno, Kazuaki Ninomiya, Kenji Takahashi, Masayuki Inui, Yota Tsuge
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Takahisa Kogure, Masako Suda, Kazumi Hiraga, Masayuki Inui
Summary: Protocatechuic acid (PCA) is a natural bioactive phenolic acid that can be produced by Corynebacterium glutamicum through the DHS and 4-HBA pathways. Simultaneous utilization of both pathways is an efficient method for PCA production, with the highest reported productivity reaching 82.7 g/L.
METABOLIC ENGINEERING
(2021)
Article
Biochemical Research Methods
Shiori Nakazawa, Osamu Imaichi, Takahisa Kogure, Takeshi Kubota, Koichi Toyoda, Masako Suda, Masayuki Inui, Kiyoto Ito, Tomokazu Shirai, Michihiro Araki
Summary: The technique involves computational suggestion of genetic modifications based on design history, retrieval of well-known designs related to the history, and enhancement of production through experimental modification. The successful utilization of former cycles suggests relevant designs worth testing, ultimately aiding in the development of bioprocessing.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Physical
Haruhiko Teramoto, Tetsu Shimizu, Masako Suda, Masayuki Inui
Summary: This study explored the genetic engineering of Escherichia coli for hydrogen production. The introduction of exogenous hydrogenase and inactivation of endogenous hydrogenases resulted in both hydrogen production and uptake. Disabling the pathways for ethanol, lactate, and succinate production significantly increased hydrogen production. Unexpectedly, high expression of exogenous hydrogenase completely repressed hydrogen production, and the introduction of heterologous enzyme repressed the endogenous hydrogen production ability.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Microbiology
Koichi Toyoda, Riki Sugaya, Akihiro Domon, Masako Suda, Kazumi Hiraga, Masayuki Inui
Summary: Understanding the metabolic pathways and regulation of amino acids is crucial for optimizing amino acid production. This study identified and characterized the ans operon that is involved in the catabolism of L-Asn in Corynebacterium glutamicum R. The operon consists of genes encoding a transcriptional regulator (AnsR), asparaginase (AnsA2), aspartase (AspA2), and permease (AnsP). The enzymes and permease encoded by this operon are essential for the utilization of L-Asn. The study also revealed the regulatory mechanism of the operon, with AnsR acting as a transcriptional repressor that is inhibited by L-Asn.
Article
Biotechnology & Applied Microbiology
Tetsu Shimizu, Haruhiko Teramoto, Masayuki Inui
Summary: This study provides a novel approach for increasing the yield of photofermentative H-2 production from acetate by purple nonsulfur phototrophic bacteria. This study further suggests that polyhydroxyalkanoate is not only a storage substance for carbon and energy in bacteria, but may also act as a signaling molecule that mediates bacterial metabolic adaptations to specific environments.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Chemistry, Physical
Haruhiko Teramoto, Masako Suda, Masayuki Inui
Summary: This study investigates the inhibitory compounds in dilute sulfuric acid-pretreated corn stover on hydrogen production by Escherichia coli. The results indicate that acetate is the primary inhibitory factor.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
