Review
Chemistry, Multidisciplinary
Olga A. Kamanina, Evgeniya A. Saverina, Pavel Rybochkin, Vyacheslav A. Arlyapov, Anatoly N. Vereshchagin, Valentine P. Ananikov
Summary: Microorganism-cell-based biohybrid materials have gained significant attention and have been applied in various fields. Sol-gel technology is a preferred technique for creating high-purity organic-inorganic matrices for biocomponent immobilization. This review focuses on the synthesis and application of hybrid sol-gel materials obtained by encapsulating microorganism cells in inorganic matrices. Techniques for increasing microorganism effective time and the potential use of sol-gel hybrid materials in catalysis are discussed.
Article
Chemistry, Physical
Dan Wu, Wenming Zhang, Baihe Fu, Zhonghai Zhang
Summary: Biological hydrogen production has great potential in the field of renewable energy, and this research demonstrates the significant enhancement of biological hydrogen production through solar-light induction and intra-cellular inorganic-biological hybrid system optimization.
Article
Chemistry, Analytical
Feng Qin, Shujuan Wang, Mingxia Gao, Xiangmin Zhang
Summary: This study developed a rapid and sensitive detection method for Staphylococcus aureus and Klebsiella pneumoniae using bacitracin immobilized on magnetic nanoparticles. The method decreased the detectable concentration of bacteria by 2-3 orders of magnitude in unenriched samples, and the enrichment and identification can be completed in one hour. The bacitracin-functionalized magnetic composite shows potential for large-scale enrichment and isolation of target pathogens from complex biological samples.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Multidisciplinary
Hanyu Qian, Eric Mclamore, Nikolay Bliznyuk
Summary: Reuse of alternative water sources for irrigation is a sustainable approach to reduce water gaps and increase food production, but it poses a risk of bacterial contamination. This study developed a statistical machine-learning framework to accurately predict the concentration of pathogenic organisms, such as E. coli, in untreated surface water using an impedimetric electrochemical aptasensor. The framework improved prediction accuracy and can be used for rapid decision support in hydroponic irrigation.
Article
Multidisciplinary Sciences
Delia Tomoiaga, Jaclyn Bubnell, Liam Herndon, Paul Feinstein
Summary: Griffith proposed the concept of DNA transfer between bacteria in 1928. Researchers have demonstrated that E. coli can accept multiple plasmids simultaneously, resulting in widespread aclonality during colony development. Tracking clonally related bacteria allows for mosaic analysis of gene function. Additionally, it was discovered that clonally related bacterium rods self-organize fractally and can remain linked during colony growth, presenting a potential target for controlling microbiota growth.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Haoran Li, Yubo Wang, Yaohua Li, Wei Wang
Summary: The new method successfully quantifies bacterial viability at a single-cell level using optical tweezers, holding potential in antibiotic susceptibility testing, drug screening, and rapid diagnostics.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Haoran Li, Yubo Wang, Yaohua Li, Wei Wang
Summary: This study presents a new label-free bacterial viability assay based on optical tweezers technology, which quantifies bacterial viability by monitoring bacterial nanomotion in the optical traps. The assay is characterized by short time consumption, simple sample preparations, and no cytotoxicity, making it a reliable method for assessing bacterial viability at the single-cell level.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Christopher J. Anderson, Christopher B. Medina, Brady J. Barron, Laura Karvelyte, Tania Love Aaes, Irina Lambertz, Justin S. A. Perry, Parul Mehrotra, Amanda Goncalves, Kelly Lemeire, Gillian Blancke, Vanessa Andries, Farzaneh Ghazavi, Arne Martens, Geert van Loo, Lars Vereecke, Peter Vandenabeele, Kodi S. Ravichandran
Summary: The study demonstrates how nutrients released from apoptotic cells can promote the growth of Enterobacteriaceae and highlights the role of pyruvate formate-lyase-encoding pflB gene in bacterial colonization in various contexts. These findings provide new insights into host-pathogen interactions and have implications for gut inflammation and chemotherapy treatment.
Review
Chemistry, Multidisciplinary
Bolin An, Yanyi Wang, Yuanyuan Huang, Xinyu Wang, Yuzhu Liu, Dongmin Xun, George M. Church, Zhuojun Dai, Xiao Yi, Tzu-Chieh Tang, Chao Zhong
Summary: Engineered living materials (ELMs) are a new form of materials composed of living matter or cell communities embedded in self-regenerating matrices or artificial scaffolds. Similar to natural materials like bone and skin, ELMs possess the functional capabilities of living organisms, including the ability to grow, self-organize, self-repair, and perform programmed biological functions upon sensing external cues. ELMs show promise in areas such as green energy production, bioremediation, disease treatment, and fabricating advanced smart materials.
Article
Chemistry, Physical
Sumit Kumar, Mamata Karmacharya, Issac J. Michael, Yongjun Choi, Junyoung Kim, InUn Kim, Yoon-Kyoung Cho
Summary: This study demonstrates the bridging of two membranes using supramolecular chemistry to create exosomes that act as nanoreactors for effective biocatalytic cascades, functioning for hours inside living cells and repairing damaged tissue regions by supplying ATP.
Article
Biotechnology & Applied Microbiology
Zhi-Hao Zhong, Yu-Qing Zhang
Summary: A novel E. coli cell-immobilizing polyvinyl alcohol hydrogel membrane (ECI-PVAHM) was prepared, which has high water stability, transparency, and mechanical strength, and does not affect the division, proliferation, and metabolic activity of the bacteria. This technology has potential applications in molecular biology and biopharmaceutical production.
MICROBIAL CELL FACTORIES
(2022)
Article
Agricultural Engineering
Maria J. Jimenez Vizcarra, Shaily Mahendra, Meng Wang
Summary: This study presents a novel approach to address the limitation of manganese peroxidase (MnP) in Mn2+-deficient systems by co-immobilizing MnP and Mn2+ in silica gels. The co-immobilized MnP exhibited higher activity and superior dye removal compared to MnP immobilized alone. This research enables a broader application of MnP enzymes in sustainable environmental remediation and industrial catalysis.
BIORESOURCE TECHNOLOGY
(2023)
Review
Infectious Diseases
Fabio P. Sellera, Luciano C. B. A. Da Silva, Nilton Lincopan
Summary: The COVID-19 pandemic has deepened the relationships between humans and companion animals, leading to an increase in the use of antibiotics and biocides worldwide. Data from global surveillance studies show a linear trend in increasing carbapenem resistance among Gram-negative pathogens in companion animals, with humans potentially acquiring carbapenemase-producing bacteria through contact with infected animals or contaminated environments. The rapid spread of WHO critical priority carbapenemase-producing pathogens in companion animals presents a One Health challenge for the post-pandemic world.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Muhammad Tanveer Munir, Nattar Maneewan, Julien Pichon, Mohammed Gharbia, Ismael Oumarou-Mahamane, Jessica Baude, Chantal Thorin, Didier Lepelletier, Patrice Le Pape, Matthieu Eveillard, Mark Irle, Helene Pailhories, Florence Aviat, Christophe Belloncle, Michel Federighi, Laurence Dubreil
Summary: The study used mCherry Staphylococcus aureus as a model bacterium and employed a confocal spectral laser microscope (CSLM) to characterize wood surfaces. It was found that more bacteria were present near the surface of the LT face wood, and this methodology shows promise as a tool for hygienic safety evaluation.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
James Y. Tan, Tatyana E. Saleski, Xiaoxia Nina Lin
Summary: Co-cultivation in microfluidic droplets is a versatile tool for studying microbial communities, and the droplet size is a critical parameter that affects various aspects of community dynamics.
Article
Biotechnology & Applied Microbiology
Juan Manuel Galdoporpora, Sofia Municoy, Fatima Ibarra, Virginia Puente, Pablo Edmundo Antezana, Maria Ines Alvarez Echazu, Martin F. Desimone
Summary: This study successfully obtained copper and zinc nanostructures using yerba mate extract as a reducing and capping agent. By adjusting salt concentration and temperature, the morphology of the nanostructures was tuned. The nanomaterials exhibited antimicrobial and antioxidant activities, highlighting their potential applications in various fields.
CURRENT NANOSCIENCE
(2023)
Article
Materials Science, Ceramics
Chetna C. Chauhan, Abhishek A. Gor, Tanuj Gupta, Martin F. Desimone, Neha Patni, Rajshree B. Jotania
Summary: M-type calcium hexaferrite (CaM) was synthesized using extracts from Azadirachta indica and Murraya koenigii leaves, and the presence of phytochemicals in the extracts was found to significantly influence the properties of CaM hexaferrites. The study provides insights into the structural, optical, magnetic, and dielectric characteristics of CaM hexaferrites synthesized via a greener route.
CERAMICS INTERNATIONAL
(2022)
Article
Chemistry, Multidisciplinary
Prashant B. Chouke, Kanhaiya M. Dadure, Ajay K. Potbhare, Ganesh S. Bhusari, Aniruddha Mondal, Karan Chaudhary, Virender Singh, Martin F. Desimone, Ratiram G. Chaudhary, Dhanraj T. Masram
Summary: Bioinspired delta-bismuth oxide nanoparticles were synthesized using a greener reducing agent and surfactant. The use of Crinum viviparum flower extract for the fabrication of nanoparticles was employed for the first time. The nanoparticles showed promising photocatalytic activity for the degradation of toxic dyes and also exhibited good inhibition of the New Delhi metallo-beta-lactamase 1 enzyme.
Article
Biochemistry & Molecular Biology
Maria Alvarez Echazu, Sandra J. Renou, Gisela S. Alvarez, Martin F. Desimone, Daniel G. Olmedo
Summary: In this study, a chitosan-silica-based biocomposite was synthesized and characterized for its physicochemical properties and biocompatibility. The results showed that the biocomposite had good biocompatibility and could act as a template for cellular interactions and extracellular matrix formation, promoting new bone tissue formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Fresia Melina Silva Sofras, Martin Federico Desimone
Summary: Cannabis sativa L. has a long history of being used as medicine. The medicinal properties of this plant are attributed to cannabinoids, terpenes, and flavonoids. The full-spectrum plant extracts, which contain a combination of these compounds, have shown superior medicinal effects compared to isolated compounds like CBD or THC. Chromatography methods, such as HPLC and GC, are widely used for the analysis of cannabis components. Standardized protocols and quality control policies are necessary for a comprehensive analysis of cannabis extracts and derivatives.
CURRENT PHARMACEUTICAL DESIGN
(2023)
Article
Pharmacology & Pharmacy
Luis A. Villarruel, Belen Brie, Sofia Municoy, Damasia Becu-Villalobos, Martin F. Desimone, Paolo N. Catalano
Summary: The study evaluated silica nanoparticles and silica-collagen nanocomposites for the loading and release of recombinant human growth hormone (hGH). The results showed that bare nanoparticles had higher hGH adsorption capacity compared to thiol- and isobutyl-bearing particles of similar sizes. Incorporating the protein-loaded particles into collagen matrices resulted in a progressive and prolonged hGH release profile, suggesting the potential use of biocompatible silica-collagen nanocomposites for reducing the number of periodic hGH administrations.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Infectious Diseases
Sofia Municoy, Pablo Edmundo Antezana, Martin Gonzalo Bellino, Martin Federico Desimone
Summary: UV irradiation method was used to in situ synthesize silver nanoparticles (AgNPs) within 3D-printed collagen-based scaffolds. The size and shape of AgNPs were controlled by modifying the concentration of AgNO3 and UV irradiation time. The results showed that AgNPs stabilized the scaffolds and improved the swelling capacity of collagen, and exhibited high bactericidal efficiency against both Gram-negative and Gram-positive bacteria.
Article
Chemistry, Analytical
Shivender Singh Saini, Guillermo J. Copello, M. Florencia Martini
Summary: In this study, an ultrasound-assisted solid phase extraction method utilizing a rotating cigarette filter was developed for the preconcentration and determination of trace amounts of bisphenol A in source and drinking water. High-performance liquid chromatography coupled with UV detector was used for qualitative and quantitative measurements. The sorbent-analyte interactions were investigated computationally and experimentally, and various extraction parameters were optimized. The proposed method exhibited good linearity, low detection limit, high precision, and recovery, providing a cost-effective, simple, fast, and sensitive analytical approach for the determination of bisphenol A in water samples.
ANALYTICAL SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Juan M. Minoia, Maria E. Villanueva, Guillermo J. Copello, Julian Rodriguez Talou, Alejandra B. Cardillo
Summary: A biocatalytic system was developed for the production of anisodamine and scopolamine, utilizing affinity-immobilized ChBD-H6H which retained catalytic activity in chitin matrices. Enzyme-recycling through adsorption-desorption strategies improved product yields and allowed for the simple and economical reutilization of the enzyme.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Polymer Science
Luis M. Araque, Claudio J. Perez, Antonia Infantes-Molina, Enrique Rodriguez-Castellon, Guillermo J. Copello, Juan M. Lazaro-Martinez
Summary: Two responsive PEI hydrogels (P1.5E and P2E) with different cross-linking degrees were synthesized using a click reaction and without organic solvents. The hydrogel's swelling and rheological behavior were dependent on the cross-linking degree, and the hydrogels showed stimuli-responsive behavior to pH or ionic strength changes. The hydrogels also exhibited adsorption capacity for methyl orange (MO) dye and Cu2+ ions, with P1.5E showing higher sorption capacities. Germination experiments with lettuce seeds confirmed that the hydrogels did not release toxic molecules.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Chemistry, Analytical
Shivender Singh Saini, Guillermo J. Copello, Solange Binotto Fagan, Mariana Zancan Tonel
Summary: A comparative investigation into the extraction of bisphenol A using alpha, beta, and gamma cyclodextrins was performed with computational and spectroscopic assistance. A mu-solid-phase extraction podium and HPLC-UV were used for extraction and analysis. Beta-cyclodextrin was selected as the adsorbent for bisphenol A extraction based on density functional theory calculations and spectroscopy data. Optimization of various parameters led to a limit of detection of 0.70 ng/ml and a limit of quantification of 2.31 ng/ml with beta-cyclodextrin in real source water samples, demonstrating the development of a novel, simple, sensitive, and economic analytical method.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Polymer Science
Eliezer Velasquez, Cristian Patino Vidal, Guillermo Copello, Carol Lopez de Dicastillo, C. J. Perez, Abel Guarda, Maria Jose Galotto
Summary: This study determined the effect of incorporating fumed nanosilica on the processability improvement of post-consumer recycled flexible polypropylene. The addition of nanosilica increased the thermal stability and crystallinity of the polymer, improving the processability of the nanocomposites. However, it did not affect the crystallization and melting temperatures of the polymer.
Article
Polymer Science
Eliezer Velasquez, Carol Lopez de Dicastillo, Cristian Patino Vidal, Guillermo Copello, Cristopher Reyes, Abel Guarda, Maria Jose Galotto
Summary: The food industry faces the challenge of increasing the recycling of post-consumer plastics, especially flexible polypropylene used in food packaging. This research evaluated the feasibility of incorporating fumed nanosilica to improve the properties of recycled flexible polypropylene films. The results showed that the addition of nanosilica improved the mechanical properties and seal strength of the films, but it did not impact the permeability properties. However, the overall migration of the nanocomposites exceeded the limit allowed by European legislation.
Article
Pharmacology & Pharmacy
Fresia Melina Silva Sofras, Rosario Alonso, Daiana Sabrina Retta, Paola Di Leo Lira, Martin Federico Desimone, Catalina Maria van Baren
Summary: This study developed and validated a simple and rapid HPLC-UV method for the identification and quantification of principal cannabinoids in cannabis products. The method was found to have comparable performance to more sophisticated analytical instruments for cannabinoid analysis. The method exhibited linear concentration-response relationship with R^2 values greater than 0.99 for all 7 cannabinoids.
CURRENT PHARMACEUTICAL DESIGN
(2023)
Article
Polymer Science
Mayte M. Quispe, Maria E. Villanueva, Guillermo J. Copello, Olivia V. Lopez, Marcelo A. Villar
Summary: In this study, plasticized PHB functionalized with copper (II) sulfate was successfully prepared, yielding biodegradable antimicrobial flexible films. Addition of polymer plasticizer and copper (II) sulfate significantly improved the surface roughness of the antimicrobial films and the diffusion of copper (II) sulfate. The films exhibited inhibitory effects against both Gram-positive and Gram-negative bacteria, while also showing no cytotoxicity in vitro.
