Article
Engineering, Marine
Vincenzo Zammuto, Eleonora Agostino, Angela Macri, Antonio Spano, Emanuela Grillo, Marco Sebastiano Nicolo, Concetta Gugliandolo
Summary: EPS and BS produced by marine Bacillus licheniformis have different effects on bacterial adhesion and biofilm disruption. The combination of these two exopolymers showed synergistic effects and effectively reduced the attachment and biofilm formation of Pseudomonas aeruginosa and Staphylococcus aureus on various surfaces.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
Article
Biology
Jorge Gracida, Ana Corona, Aldo Amaro, Arturo Abreu, Evelyn Zamudio
Summary: The present study investigated the use of a biosurfactant produced by Bacillus cereus in soil washing for crude oil removal. Different carbon sources and NaCl concentrations were evaluated to obtain the biosurfactant. Bacillus cereus strain was isolated from saline soil. Crude oil, glucose, and glycerol were used as carbon sources in the culture media. The effect of NaCl concentration on surface tension was evaluated using the separated carbon sources. The washing capacity of the biosurfactant-containing culture supernatant was compared with synthetic surfactant and water in contaminated soils, showing a higher removal efficiency.
Article
Biotechnology & Applied Microbiology
Peter Klausmann, Katja Hennemann, Mareen Hoffmann, Chantal Treinen, Moritz Aschern, Lars Lilge, Kambiz Morabbi Heravi, Marius Henkel, Rudolf Hausmann
Summary: Bacillus subtilis 3NA, a strain capable of high cell densities, utilized its surfactin-producing variant JABs32 in fed-batch processes to achieve significantly increased surfactin titers. Optimization of culture conditions showed a promising potential for enhanced surfactin production in high cell density fermentation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biophysics
Mareen Hoffmann, Denise Mueck, Lutz Grossmann, Lena Greiner, Peter Klausmann, Marius Henkel, Lars Lilge, Jochen Weiss, Rudolf Hausmann
Summary: Surfactin, a biosurfactant produced by Bacillus subtilis, exhibits high interfacial activity and emulsion forming properties, making it a strong candidate for O/W emulsification. It is stable within the pH range of 6 to 9, but destabilizes with CaCl2 addition (>3mM).
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Dominika Ciurko, Zaneta Czyznikowska, Anna Kancelista, Wojciech Laba, Tomasz Janek
Summary: The microbial conversion of agro-industrial oil wastes into biosurfactants using Bacillus subtilis #309 shows promise as a biomass refinery approach. Surfactin production using rapeseed and sunflower cakes was analyzed, and the surfactin concentration, pH, surface tension, and emulsification index were measured. The surfactin concentration reached 1.19 +/- 0.03 g/L in sunflower cake medium and 1.45 +/- 0.09 g/L in rapeseed cake medium. The surfactin exhibited antioxidant activity and significant angiotensin-converting enzyme (ACE)-inhibitory activity. The ACE-inhibitory lipopeptide biosurfactant shows promise to be used as a natural antihypertensive agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Chemical
Italo Waldimiro Lima de Franca, Darlane Wellen Freitas de Oliveira, Maria Estela Aparecida Giro, Vania Maria Maciel Melo, Luciana Rocha Barros Goncalves
Summary: By evaluating the kinetics of lipopeptide biosurfactant production by Bacillus subtilis LAMI005 and its tensoactive and emulsifying properties, it was found that the biosurfactant showed high surface tension reduction and emulsifying capabilities when the appropriate nitrogen and carbon sources were provided in a mineral medium. The surfactin exhibited stable emulsification in various oils, indicating its potential application in high salt environments such as oil spills in marine areas.
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
(2022)
Article
Environmental Sciences
Isabela Maria Monteiro Vieira, Brenda Lohanny Passos Santos, Lucas Santos Silva, Larissa Castor Ramos, Roberto Rodrigues de Souza, Denise Santos Ruzene, Daniel Pereira Silva
Summary: Pineapple peel, rich in nutrients, can be used for biosurfactant production, reducing costs and positively impacting the environment.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Microbiology
Bo Wu, Jianlong Xiu, Li Yu, Lixin Huang, Lina Yi, Yuandong Ma
Summary: This study aimed to investigate the promotion effect of defined co-culture of Bacillus subtilis and Pseudomonas aeruginosa on the biodegradation of crude oil. The results showed that after 3 days of defined co-culture, the residual oil and saturated and aromatic fraction in the samples were significantly reduced, and the degradation efficiency of crude oil was improved from 32.61% and 54.35% in individual culture to 63.05%. Furthermore, the defined co-culture system exhibited relatively excellent performance in bacterial growth, cell surface hydrophobicity, and emulsification activity. These findings suggest that the combination of Bacillus subtilis and Pseudomonas aeruginosa can effectively promote the degradation and utilization of crude oil.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Energy & Fuels
Masoumeh Amiri, Mobeen Fatemi, Ebrahim Biniaz Delijani
Summary: The study investigates the effects of the hybrid application of low-salinity and polymer on the properties of brine/rock and brine/oil interfaces. Experimental results show that the aqueous salinity and polymer concentration have an influence on the interfacial tension, wettability, and zeta potentials.
Article
Engineering, Chemical
Sidra Hashmi, Sajid Iqbal, Iftikhar Ahmed, Hussnain Ahmed Janjua
Summary: Proteases that can remain active under extreme conditions are widely applicable in the commercial sector. This study isolated 142 bacterial isolates from alkaline soil, with Bacillus subtilis S1 and Bacillus amyloliquefaciens KSM12 identified as the top protease producers. These isolates secreted proteases that were suitable for industries operating in highly alkaline environments.
Article
Energy & Fuels
Vorasate Thanasaksukthawee, Nipada Santha, Schradh Saenton, Nakorn Tippayawong, Pirat Jaroonpattanapong, Jalal Foroozesh, Suparit Tangparitkul
Summary: In this study, the potential of structural trapping and CO2 leakage in CO2 geological storage were experimentally investigated. The contribution of reservoir rock characteristics to CO2 storage potential was found to be significant, highlighting the importance of considering both seal and reservoir rocks. The dissolution of CO2 in formation brine was also studied, indicating a decrease in storage potential due to rock dissolution. The study provides a more comprehensive analysis approach for CO2 storage.
Article
Biology
Annsy Arredondo-Nunez, Gisele Monteiro, Carol N. Flores-Fernandez, Lina Antenucci, Perttu Permi, Amparo Iris Zavaleta
Summary: This study presents a novel type II L-asparaginase isolated from halotolerant Bacillus subtilis CH11 in Peru, which was successfully expressed and purified using a heterologous system. The enzyme showed high catalytic activity and holds potential for future industrial applications.
Article
Energy & Fuels
Varsha Singh
Summary: Bacillus aryabhattai SPS1001 grown in a nutrient broth medium supplemented with waste coal tar demonstrated high production of biosurfactant with excellent emulsification and stabilization properties, making it suitable for applications such as bitumen recovery from oil sands.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Chemistry, Multidisciplinary
Gianmaria Oliva, Luca Di Stasio, Giovanni Vigliotta, Francesco Guarino, Angela Cicatelli, Stefano Castiglione
Summary: Soil salinity negatively impacts plant growth by altering water potential and nutrient uptake. This study characterized novel halotolerant/halophilic bacteria and evaluated their plant growth-promoting features and regulation by NaCl. The results showed strain-dependent differences in plant growth-promoting activities and the influence of NaCl on these activities.
APPLIED SCIENCES-BASEL
(2023)
Article
Microbiology
Abdussabur M. Kaleh, Pooja Singh, Purabi Mazumdar, Kah Ooi Chua, Jennifer A. Harikrishna
Summary: In this study, halotolerant bacteria were isolated and characterized from a Malaysian mangrove forest. The bacteria were found to promote the growth of banana plants and reduce salt stress, with enhanced antioxidant enzyme activity and reduced vulnerability.
MICROBIOLOGICAL RESEARCH
(2022)