Article
Biotechnology & Applied Microbiology
Amy T. Siceloff, Doug Waltman, Nikki W. Shariat
Summary: Poultry remains a significant source of foodborne salmonellosis, despite a significant reduction in Salmonella incidence during processing. Regional differences in Salmonella serovar prevalence were observed, and the limited detection depth of traditional culture techniques contributed to discrepancies between pre- and postharvest poultry. Accurately identifying all serovars present is crucial for assessing public health risk and implementing effective Salmonella controls.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Abdul Basit, Hamza Tahir, Zulquernain Haider, Hafsa Tariq, Asim Ullah, Shafiq Ur Rehman
Summary: This study successfully deleted the SpvB gene from the virulent plasmid of Salmonella Gallinarum using the CRISPR/Cas9 method, further demonstrating the significant role of this gene in causing the disease and providing a new approach for preparing an effective vaccine strain to control fowl typhoid in poultry.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Food Science & Technology
Kirsten Wessels, Diane Rip, Pieter Gouws
Summary: Controlling Salmonella in chicken processing plants is a global challenge, with current chemical antimicrobials losing effectiveness due to rising resistance. Bacteriophages are gaining appeal as an alternative method, offering host specificity and potential for successful implementation in large-scale operations.
Article
Biotechnology & Applied Microbiology
Matthew W. Quinn, Nicola F. Linton, Carlos G. Leon-Velarde, Shu Chen
Summary: Current detection protocols for Salmonella serovars are limited and often miss less abundant serovars in a sample. However, the CRISPR-SeroSeq method can accurately detect multiple serovars without the need for culture isolation. This method successfully detected 34 serovars in pure cultures and 25 different serovars in environmental samples from poultry farms, highlighting its effectiveness in surveillance.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Hang Pan, Chenghao Jia, Narayan Paudyal, Fang Li, Junyong Mao, Xi Liu, Chenghang Dong, Kun Zhou, Xiayi Liao, Jiansen Gong, Weihuan Fang, Xiaoliang Li, Corinna Kehrenberg, Min Yue
Summary: In this study, 12 typing methods were evaluated for their effectiveness in routine monitoring of foodborne Salmonella transmission. The CRISPR-MVLST method showed higher discriminatory power and the ability to track the source of Salmonella strains along the food chain, making it a valuable tool for controlling the spread of this pathogen.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Haojie Ge, Chao Lin, Yanping Xu, Maozhi Hu, Zhengzhong Xu, Shizhong Geng, Xin 'An Jiao, Xiang Chen
Summary: Phage LP31, as a lytic phage, has been identified as an effective control agent for Salmonella and other foodborne bacteria. LP31 possesses good adhesion properties and can remove biofilms formed by Salmonella. These characteristics make LP31 a potential biocontrol agent for preventing the spread of Salmonella.
VETERINARY MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Matias Aguilera, Eduardo Tobar-Calfucoy, Victoria Rojas-Martinez, Rodrigo Norambuena, Maria Jesus Serrano, Onix Cifuentes, Maria Sofia Zamudio, Daniel San Martin, Pabla Lara, Andrea Sabag, Marcela Zabner, Daniel Tichy, Pamela Camejo, Luis Leon, Michael Pino, Soledad Ulloa, Felipe Rojas, Christian Pieringer, Cecilia Muster, Daniel Castillo, Nicolas Ferreira, Camilo Avendano, Mauro Canaval, Hans Pieringer, Pablo Cifuentes, Nicolas Cifuentes Munoz
Summary: In this study, we isolated and characterized 47 bacteriophages that showed variable degrees of lytic activity against 258 Salmonella isolates from a commercial broiler company in Brazil. Three of these bacteriophages were selected to assemble a cocktail, which was found to be highly effective against multiple serovars of Salmonella in vitro, including under conditions that resemble the chicken gut. The cocktail also showed limited or no cross-resistance, highlighting its potential for in vivo application in commercial broiler farms.
Article
Agriculture, Dairy & Animal Science
Peichao Chen, Qiang Huang, Fangzhou Cheng, Pan Sun, Qian Peng
Summary: This study investigated the contamination, serotype distribution, and drug resistance of Salmonella in poultry meat in Jiading District, Shanghai. Poultry samples were collected from commercial markets and Salmonella contamination was identified through serotype analysis. The results showed varying detection rates of Salmonella across different poultry types and years. The majority of Salmonella serotypes were S. Typhimurium, S. Enteritidis, and S. Agona. High resistance rates were observed for certain antibiotics, while low resistance was found for cefotaxime and imipenem. The study emphasizes the seriousness of Salmonella contamination in raw poultry meat and the increasing drug resistance, suggesting the need for strengthened monitoring and control measures.
Article
Biotechnology & Applied Microbiology
Diessy Kipper, Renato H. Orsi, Laura M. Carroll, Andrea K. Mascitti, Andre F. Streck, Andre S. K. Fonseca, Nilo Ikuta, Eduardo C. Tondo, Martin Wiedmann, Vagner R. Lunge
Summary: The study assessed the population structure, recent temporal evolution, and important genetic characteristics of multidrug-resistant Salmonella enterica serovar Heidelberg isolated from Brazilian poultry farms, revealing a monophyletic group with antibiotic resistance genes and virulence factors. This lineage poses a threat to Brazilian and global poultry operations.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Food Science & Technology
Matias Aguilera, Sofia Martinez, Mario Tello, Maria Jose Gallardo, Veronica Garcia
Summary: Foodborne diseases are a significant concern in public health due to their impact and the number of affected individuals each year. The food industry has implemented microbiological control plans, but new control systems are needed due to high prevalence. This study suggests that the use of bacteriophages can reduce contamination of Salmonella in chicken meat.
Article
Food Science & Technology
Andrea Zendrini, Valentina Carta, Virginia Filipello, Laura Ragni, Elena Cosciani-Cunico, Sara Arnaboldi, Barbara Bertasi, Niccolo Franceschi, Paolo Ajmone-Marsan, Dario De Medici, Marina Nadia Losio
Summary: Salmonella and Campylobacter screening in poultry meat can be efficiently conducted using real-time PCR even after shorter enrichment times, providing timely detection of these pathogens. Additionally, colorimetric LAMP is a valid alternative to real-time PCR when one-day results are needed, enabling quick identification of positive meat batches without the need for specialized instrumentation.
Article
Food Science & Technology
Savannah F. Applegate, April K. Englishbey, Tyler P. Stephens, Marcos X. Sanchez-Plata
Summary: The USDA FSIS does not have a zero-tolerance policy for Salmonella in poultry and poultry products, but has established performance standards for acceptable percentages of positive samples. The prevalence of Salmonella in poultry establishments is published for public viewing based on verification sampling results. Quantification using RT-PCR is proposed as a valuable method to determine the efficacy of interventions and make rapid food safety decisions.
Article
Agriculture, Dairy & Animal Science
H. Zeng, K. De Reu, S. Gabriel, W. Mattheus, L. De Zutter, G. Rasschaert
Summary: The study in 5 Belgian poultry slaughterhouses found that Salmonella was still present on the slaughter line despite cleaning and disinfection, with contamination found in neck skin and thigh samples. The most common serovar was Salmonella Infantis. Contamination sources included incorrect negative status of flocks and cross-contamination from previous slaughters.
Article
Agriculture, Dairy & Animal Science
C. Marin, M. Cerda-Cuellar, S. Gonzalez-Bodi, L. Lorenzo-Rebenaque, S. Vega
Summary: Salmonellosis is a major foodborne zoonosis in Europe, with poultry products as the main source of infection. The slaughterhouse has been identified as a potential source of Salmonella contamination in the poultry meat. A study was conducted to investigate a slaughterhouse with persistent Salmonella problems and found a high genetic relationship among Salmonella strains isolated during the slaughter process. It is necessary to implement molecular diagnosis methods at the field level to control Salmonella and prevent its entry into the slaughterhouse environment.
Article
Agriculture, Dairy & Animal Science
Jan Torres-Boncompte, Pablo Catala-Gregori, Josep Garcia-Llorens, Jose M. Soriano, Sandra Sevilla-Navarro
Summary: This paper explores the possibility of using the ISO 6579-1:2017 standard as a protocol for simultaneous isolation of Salmonella and bacteriophages. Two experiments were conducted, and the results showed that this method is feasible. Furthermore, a correlation was found between the antigenic formulae of the bacteria and the host range of the phages.
Article
Biotechnology & Applied Microbiology
Abigail M. Deaven, Christina M. Ferreira, Elizabeth A. Reed, Jeremy R. Chen See, Nora A. Lee, Eduardo Almaraz, Paula C. Rios, Jacob G. Marogi, Regina Lamendella, Jie Zheng, Rebecca L. Bell, Nikki W. Shariat
Summary: The study reveals a high level of Salmonella diversity and prevalence in the Susquehanna River basin, with seasonal differences in serovar distribution. Whole-genome sequencing showed diverse strains within the same serovar. The presence of multiple serovars in each sample highlights the complexity of Salmonella ecology in freshwater systems.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
N. W. Shariat, K. M. Feye, A. K. Richards, B. Booher, Z. Flores, P. M. Rubinelli, E. G. Olson, S. C. Ricke
Summary: The study aimed to investigate the incidence and diversity of Salmonella in commercial animal feeds in the US and identified Infantis and Tennessee as the most common serovars. Serogroups O:4 and O:7 were found to be enriched in the feed samples, indicating their better adaptation to low moisture animal feeds. The use of CRISPR analysis was shown to be effective in determining serovar type.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Nikki W. Shariat, Ruth E. Timme, Abigail T. Walters
Summary: Salmonella enterica subspecies arizonae displays unique evolutionary patterns revealed through whole-genome sequencing data and core genome phylogenetic analysis, including polyphyly, high conservation of antigens, presence of prophages and plasmids, and specific pathogenic islands. These characteristics make subspecies arizonae a distinct lineage within the highly diverse species of Salmonella.
MICROBIAL GENOMICS
(2021)
Article
Microbiology
Amy T. Siceloff, Naomi Ohta, Keri N. Norman, Guy H. Loneragan, Bo Norby, H. Morgan Scott, Nikki W. Shariat
Summary: Salmonella enterica can exist in food animals as multiserovar populations, and different serovars can harbor diverse antimicrobial resistance profiles. Conventional Salmonella isolation only assesses AMR in the most abundant members of a multiserovar population, potentially overlooking the AMR in less abundant serovars.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Tomi Obe, Mark E. Berrang, Nelson A. Cox, Sandra L. House, Nikki W. Shariat
Summary: The study compared different selective enrichment protocols and differential plating agars for Salmonella recovery from broiler carcass rinses. Results showed that the choice of pre-enrichment method, selective enrichment medium, and differential agar can influence the recovery of Salmonella from poultry samples. High concordance in recovery on certain agar plates was observed, highlighting the importance of careful selection of media for Salmonella detection and isolation.
JOURNAL OF FOOD SAFETY
(2021)
Article
Biotechnology & Applied Microbiology
Yezhi Fu, Nkuchia M. M'ikanatha, Jeffrey M. Lorch, David S. Blehert, Brenda Berlowski-Zier, Chris A. Whitehouse, Shaoting Li, Xiangyu Deng, Jared C. Smith, Nikki W. Shariat, Erin M. Nawrocki, Edward G. Dudley
Summary: Salmonella enterica serovar Typhimurium isolates from wild birds in the United States exhibit distinct lineages and virulence gene signatures, indicating host adaptation. These isolates are genetically different from those originating from domestic animal sources and can be attributed to wild birds with improved accuracy using genomic data and machine learning classifiers. The findings highlight the importance of wild birds as reservoirs of S. Typhimurium and provide valuable information for source attribution and epidemiological surveillance.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tomi Obe, Amber K. Richards, Nikki W. Shariat
Summary: The study aimed to evaluate the ability of different serovars of Salmonella to form biofilm under different conditions. Results showed that Salmonellae differ in attachment depending on the surface and temperature conditions encountered, which may impact their persistence in processing environments.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Amy T. Siceloff, Doug Waltman, Nikki W. Shariat
Summary: Poultry remains a significant source of foodborne salmonellosis, despite a significant reduction in Salmonella incidence during processing. Regional differences in Salmonella serovar prevalence were observed, and the limited detection depth of traditional culture techniques contributed to discrepancies between pre- and postharvest poultry. Accurately identifying all serovars present is crucial for assessing public health risk and implementing effective Salmonella controls.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Nikki W. Shariat, Bryan R. Larsen, Chery Schaeffer, Kurt E. Richardson
Summary: This study found that animal feed samples can contain multiple serovars of Salmonella, including some serovars associated with human illness. Feed ingredients exhibit higher serovar diversity. The results obtained using different Salmonella isolation methods were largely concordant.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Surendra Rasamsetti, Mark E. Berrang, Nelson A. Cox, Nikki W. Shariat
Summary: This study found that the use of selective pre-enrichment step can reduce the time required for Salmonella detection without negatively affecting the results and serovar distribution. Additionally, the incidence of Salmonella significantly decreased after antimicrobial intervention.
Review
Parasitology
Erin A. Schroeder, Michael E. Chirgwin, Emily R. Derbyshire
Summary: Plasmodium parasites alter host hepatocyte to access nutrients and evade elimination, supporting rapid replication.
TRENDS IN PARASITOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tomi Obe, Amy T. Siceloff, Megan G. Crowe, H. Morgan Scott, Nikki W. Shariat
Summary: Although the incidence of Salmonella contamination in poultry products has decreased, there has been no reduction in cases of human salmonellosis linked to poultry consumption. Improved efforts are needed to target serovars that can survive antimicrobial interventions and cause illness, as well as to reduce contamination during processing. Molecular enumeration approaches combined with deep serotyping can rapidly detect and quantify Salmonella in samples, providing a framework to assess the risk associated with poultry flocks. This study identified multiple serovars and multidrug resistant isolates, and developed a risk scoring system based on the presence of key performance indicators and the quantity of Salmonella.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Surendra Rasamsetti, Nikki W. Shariat
Summary: Salmonella contamination of poultry remains a food safety challenge for broiler processors. This study demonstrated the efficacy of antimicrobial interventions in reducing Salmonella in commercial broiler processing plants and assessed changes in Salmonella serovars before and after the chilling process.
Article
Microbiology
Richard J. Meinersmann, Mark E. Berrang, Nikki W. Shariat, Amber Richards, William G. Miller
Summary: This study found no evidence of genetic material sharing between Campylobacter isolated from chicken ceca and river water in the same watershed. The differentiation between the subpopulations is likely due to geospatial isolation, genetic drift, and the action of CRISPRs and restriction enzymes.
MICROBIOLOGY SPECTRUM
(2023)
Article
Cell Biology
Hsin- Huang, Yue Xue, Mark L. Jewell, Chin Yee Tan, Barbara Theriot, Nupur Aggarwal, Jacob Dockterman, Yang-Ding Lin, Erin A. Schroeder, Donghai Wang, Na Xiong, Jorn Coers, Mari L. Shinohara, Neeraj K. Surana, Gianna Elena Hammer
Summary: This study reveals the binary regulation of y817 cells by microbiota, where microbiota instruct in situ IL-17 production and concomitant expression of the inhibitory receptor PD-1. Microbiota-driven PD-1 inhibits in situ IL-17 production by mucosa-resident y817 effectors, linking microbiota to their simultaneous activation and suppression.