Article
Biotechnology & Applied Microbiology
Mengyun Kou, Zhenzhen Cui, Jing Fu, Wei Dai, Zhiwen Wang, Tao Chen
Summary: In this study, a C. glutamicum strain was engineered to efficiently produce optically pure (2R,3R)-2,3-butanediol. Through genetic modification and fermentation engineering, the titer of (2R,3R)-2,3-butanediol reached a record-high level, making C. glutamicum a competitive candidate for commercial production of this compound.
MICROBIAL CELL FACTORIES
(2022)
Article
Biochemistry & Molecular Biology
Daniel Tinoco, Aline M. de Castro, Lucy Seldin, Denise M. G. Freire
Summary: This study successfully increased the production of 2,3-butanediol by Paenibacillus polymyxa PM 3605 using biodiesel-derived glycerol and sugarcane molasses. The highest titer of 2,3-BDO produced from crude glycerol with the use of a secondary substrate has been achieved, showing promising potential for further improvement in the future.
PROCESS BIOCHEMISTRY
(2021)
Article
Agricultural Engineering
Christopher Chukwudi Okonkwo, Victor Ujor, Thaddeus Chukwuemeka Ezeji
Summary: LB is a cost-effective substrate for producing 2,3-BD, with P. polymyxa showing potential for efficient fermentation of non-detoxified WSH. The study revealed P. polymyxa's ability to co-utilize LB-derived sugars and exhibit tolerance to LDMICs, with promising results in 2,3-BD production and in situ detoxification.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Microbiology
Analia Borges Folle, Bruna Campos de Souza, Caroline Reginatto, Sabrina Carra, Mauricio Moura da Silveira, Eloane Malvessi, Aldo Jose Pinheiro Dillon
Summary: This study evaluated the potential application of the non-pathogenic bacterium Paenibacillus polymyxa ATCC 842 in producing the optically active isomer (R,R)-2,3-butanediol. The use of a commercial yeast extract Nucel(R) as an organic nitrogen and vitamin source, along with different medium compositions and airflow rates, was investigated. The results showed that a medium formulated with crude yeast extract and an airflow rate of 0.2 vvm resulted in a higher fermentation yield compared to the standard medium with an airflow rate of 0.5 vvm. Furthermore, this condition proved to be a good alternative for producing (R,R)-2,3-butanediol at high concentrations and with a high fermentation yield.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Jung-Hyun Ju, Min-Ho Jo, Sun-Yeon Heo, Min-Soo Kim, Chul-Ho Kim, Narayan Chandra Paul, Hyunkyu Sang, Baek-Rock Oh
Summary: In this study, an effective strategy for the biological production of highly pure R,R-2,3-butanediol (R,R-2,3-BDO) by Paenibacillus polymyxa fermentation was developed. The fermentation strategy demonstrated that high-purity R,R-2,3-BDO can be produced at high concentrations using P. polymyxa. The fermented broth containing highly pure R,R-2,3-BDO significantly stimulated the growth of soybean and strawberry seedlings.
MICROBIAL CELL FACTORIES
(2023)
Review
Microbiology
Sarah Langendries, Sofie Goormachtig
Summary: Paenibacillus polymyxa is a versatile bacterium with potential applications in promoting plant growth, providing biocontrol, and protecting against abiotic stresses. Its association with plants and diverse interactions make it an interesting research topic for studying host-microbe communications and the influence of the environment on these interactions.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Yunhee Choi, Yong-Hak Kim
Summary: This study demonstrates that cysteine residues in BdhA play a regulatory role in the production of acetoin and R-BD, depending on pH, metal binding, and oxidative stress.
JOURNAL OF MICROBIOLOGY
(2022)
Article
Engineering, Chemical
Daniel Tinoco, Chrysanthi Pateraki, Apostolis A. Koutinas, Denise M. G. Freire
Summary: This review focused on the fermentation potential of Paenibacillus species in the production of 2,3-butanediol, highlighting key fermentation parameters, metabolic pathways, and available carbon sources. The discussion also included downstream separation and purification processes for bio-based 2,3-BDO recovery, as well as the potential for high stereoisomeric purity isomers to open up new applications and markets. Paenibacillus strains were proposed as a viable alternative to biosafety level 2 strains for 2,3-BDO production.
CHEMBIOENG REVIEWS
(2021)
Article
Biotechnology & Applied Microbiology
Mostafa Elmahmoudy, Nora Elfeky, Pu Zhongji, Yue Zhang, Yongming Bao
Summary: This study isolated, expressed, and identified the R-bdh gene encoding R-BDH from Bacillus sp. DL01, showing its oxidation and reduction activities towards various substrates, as well as its activity across a wide range of temperature and pH and its modulation by different metal ions.
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wanggang Tang, Chaoqun Lian, Yu Si, Jianrong Chang
Summary: The NAD(H)-dependent (2R,3R)-BDH from the pathogen Neisseria gonorrhoeae exhibits stereospecific oxidation and reduction activity, providing insights into 2,3-BD metabolism in this pathogen and its potential impact on virulence.
MOLECULAR BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Christopher Chukwudi Okonkwo, Ademola Duduyemi, Victor Chinomso Ujor, Hasan K. Atiyeh, Ifeanyi Iloba, Nasib Qureshi, Thaddeus Chukwuemeka Ezeji
Summary: The goal of this study was to improve resource use efficiency in agricultural systems and agro-based industries, reduce wastes that go to landfills and incinerators, and consequently, improve the economics of 2,3-butanediol (2,3-BD) production. This study evaluated the feasibility of 2,3-BD production by replacing mineral nutrients, and buffers with anaerobic digestate (ADE), poultry-litter (PLBC)- and forage-sorghum (FSBC)-derived biochars. The results showed that PLBC and FSBC biochars can replace mineral nutrients and buffers in the medium, making bio-based 2,3-BD production profitably feasible.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Guangxin Huo, Maria R. Foulquie-Moreno, Johan M. Thevelein
Summary: The engineered microbial cell factories showed high production of 2,3-butanediol with the highest reported titer and close to the maximum theoretical yield, making them suitable for industrial implementation.
MICROBIAL CELL FACTORIES
(2022)
Article
Chemistry, Physical
P. Mahalakshmi, Vidyavathy Balraj, V Ragavendran
Summary: A new cocrystal PCDHB was synthesized and characterized, exhibiting excellent optical properties and stable thermal behavior. Various studies including FTIR, X-ray diffraction, and DFT analysis were conducted to understand its molecular structure and electronic transitions. The cocrystal showed promising potential in optical applications with high transparency and enhanced second harmonic generation efficiency.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Energy & Fuels
Daniel Tinoco, Denise M. G. Freire
Summary: This study aimed to produce bio-based 2,3-butanediol (2,3-BDO) using a newly discovered Paenibacillus peoriae NRRL BD-62 strain in a pilot bioreactor. The researchers achieved a high productivity and yield of 2,3-BDO using a surface aeration system and keeping the oxygen transfer rate (OTR) constant. This is the first time that 2,3-BDO has been produced by a wild-type and safe P. peoriae strain in a pilot bioreactor with a constant OTR.
Review
Biotechnology & Applied Microbiology
Priscila Esteves Faria, Aline M. Castro, Denise Maria Guimaraes Freire, Rafael D. Mesquita
Summary: This article elucidates the details of isomer production of 2,3-butanediol and acetoin, non-oxidative metabolism products of microorganisms, through bioinformatics analysis and compares the stereo-triads of other enzymes. These results are of great importance for understanding the biosynthesis and industrial production of BD/AC.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Engineering, Civil
Gang Zheng, Xingyuan Gu, Tianqi Zhang, Jibin Sun, Weihong Zheng, Qi Fan, Jingbo Tong, Yu Diao
Summary: In this study, a novel artificial intelligence method, random forest, was used to predict the vertical displacement of bridge piers above EPBM tunnels, and Naive Bayes theory was introduced for statistical analysis. The in situ observations showed successful control of EPBM variables to ensure safe passage.
EUROPEAN JOURNAL OF ENVIRONMENTAL AND CIVIL ENGINEERING
(2022)
Article
Chemistry, Physical
Sarah Rodgers, Alex Conradie, Rebekah King, Stephen Poulston, Martin Hayes, Rajesh Reddy Bommareddy, Fanran Meng, Jon McKechnie
Summary: The study demonstrates that sustainable production of commodity chemicals such as isopropanol and acetone from gasified Kraft black liquor is achievable through the utilization of supercritical water gasification and aerobic gas fermentation technology. The technoeconomic assessment shows that producing commodity chemicals priced at around US$1000 per tonne is feasible, with negative greenhouse gas emissions achieved within a cradle-to-gate life cycle assessment framework.
JOHNSON MATTHEY TECHNOLOGY REVIEW
(2021)
Editorial Material
Biotechnology & Applied Microbiology
Jiuzhou Chen, Yu Wang, Ping Zheng, Jibin Sun
Summary: Selecting desired variants from protein libraries is a challenging task in directed evolution. Engineering synthetic auxotrophs, which establish a connection between cell growth and protein properties, enables growth-coupled selection experiments that are high throughput and compatible with continuous evolution. In silico simulation-guided metabolic reprogramming can assist in the design of customized synthetic auxotrophs.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jiao Liu, Moshi Liu, Tuo Shi, Guannan Sun, Ning Gao, Xiaojia Zhao, Xuan Guo, Xiaomeng Ni, Qianqian Yuan, Jinhui Feng, Zhemin Liu, Yanmei Guo, Jiuzhou Chen, Yu Wang, Ping Zheng, Jibin Sun
Summary: In this study, the authors use CRISPR technology and CRISPRi screening to convert a wild-type Corynebacterium glutamicum to a hyperproducer of L-proline, an amino acid with various applications. By optimizing the biosynthetic enzyme and regulating the carbon flux, they achieved efficient production of L-proline.
NATURE COMMUNICATIONS
(2022)
Review
Chemistry, Physical
Vivek Narisetty, Rylan Cox, Rajesh Bommareddy, Deepti Agrawal, Ejaz Ahmad, Kamal Kumar Pant, Anuj Kumar Chandel, Shashi Kant Bhatia, Dinesh Kumar, Parmeswaran Binod, Vijai Kumar Gupta, Vinod Kumar
Summary: Biologists and engineers are working towards a sustainable society, with a focus on waste management and valorisation. Lignocellulosic biomass is a renewable feedstock for clean processes, but the underutilization of xylose presents a major bottleneck in commercializing LCB-based biorefineries. Success in converting xylose into sustainable products requires addressing challenges in microbiology, genetics, and biochemistry.
SUSTAINABLE ENERGY & FUELS
(2021)
Article
Biotechnology & Applied Microbiology
Timothy C. Cairns, Xiaomei Zheng, Claudia Feurstein, Ping Zheng, Jibin Sun, Vera Meyer
Summary: Submerged fermentation using filamentous fungal cell factories is important for producing useful molecules, and macromorphologies formed during fermentation have a critical impact on product titres and bioreactor performance. To address this, a library of conditional expression mutants in Aspergillus niger was developed, providing strains with distinct and tunable macromorphologies. The study also revealed the connection between pellet formation, strain fitness, and filamentous growth, and demonstrated how conditional expression of the kinase gene pkh2 can decouple various parameters. Further analysis of this gene and related pathways may enable more precise engineering of A. niger macromorphology.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tongtong Zhang, Jiuzhou Chen, Ping Zheng, Weimin Gong, Jibin Sun, Haiping Liu
Summary: Rp-HemA from Rhodopseudomonas palustris was found to have high activity as an ALAS enzyme. The crystal structure of Rp-HemA revealed open, half close, and close conformations. The newly observed half close conformation may also be conserved in the ALAS family. The pre-existing close and half close conformations in Rp-HemA may play a crucial role in its high activity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Nicole Pearcy, Marco Garavaglia, Thomas Millat, James P. Gilbert, Yoseb A. Song, Hassan Hartman, Craig R. Woods, Claudio Tomi-Andrino, Rajesh Reddy Bommareddy, Byung-Kwan P. Cho, David A. A. Fell, Mark D. Poolman, John R. A. King, Klaus D. Winzer, Jamie A. Twycross, Nigel P. D. Minton
Summary: This study presents the development of a genome-scale metabolic model (GSM) for C. necator H16 linked with the BioCyc database. The integration of experimental observations and omics data improves the accuracy of the model and provides insights for designing metabolic engineering strategies for C. necator H16.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xiaomei Zheng, Timothy C. Cairns, Xiaomei Ni, Lihui Zhang, Huanhuan Zhai, Vera Meyer, Ping Zheng, Jibin Sun
Summary: This study investigates the role of the pkaC gene in regulating hyphal growth and citric acid production in Aspergillus niger during fermentation. The overexpression of pkaC improves hyphal growth and increases citric acid titres. Metabolomic and transcriptomic analysis provides insights into the molecular mechanisms underlying hyperproductivity and pellet growth.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Vivek Narisetty, Ashish A. Prabhu, Rajesh Reddy Bommareddy, Rylan Cox, Deepti Agrawal, Ashish Misra, M. Ali Haider, Amit Bhatnagar, Ashok Pandey, Vinod Kumar
Summary: This study demonstrates that Yarrowia lipolytica can utilize acetate as a carbon source for growth and accumulate succinic acid. Through adaptive laboratory evolution, the recombinant strain showed improved tolerance and efficiency in utilizing acetate to produce succinic acid. Cofermentation of acetate and glucose further increased the production of succinic acid and lipids.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Lifu Song, Feng Geng, Zi-Yi Gong, Xin Chen, Jijun Tang, Chunye Gong, Libang Zhou, Rui Xia, Ming-Zhe Han, Jing-Yi Xu, Bing-Zhi Li, Ying-Jin Yuan
Summary: The study introduces a novel DNA strand assembly algorithm, DBGPS, that utilizes de Bruijn graph and greedy path search to tackle errors commonly encountered in DNA data storage. DBGPS demonstrates significant advantages in handling DNA breaks, rearrangements, and indels, and its robustness is demonstrated through various tests.
NATURE COMMUNICATIONS
(2022)
Review
Biotechnology & Applied Microbiology
Vinod Kumar, Deepti Agrawal, Rajesh Reddy Bommareddy, M. Ahsanul Islam, Samuel Jacob, Venkatesh Balan, Vijai Singh, Vijay Kumar Thakur, Naveen Kumar Navani, Nigel S. Scrutton
Summary: The circular economy will bring disruptive transformation in manufacturing technologies. Lignocellulosic biomass, an inexhaustible and alternative resource, can be used as a feedstock for biorefineries. While research has focused on the use of glucose, the potential of xylose and arabinose has been largely overlooked. This review highlights the suitability of arabinose as a starting feedstock for various chemical products and discusses the challenges and prospects associated with its production.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Timothy C. Cairns, Tom de Kanter, Xiaomei Z. Zheng, Ping Zheng, Jibin Sun, Vera Meyer
Summary: In this study, the researchers predicted six morphology and productivity associated 'morphogenes' that impact total secreted protein in A. niger using gene coexpression network data. They found that radial growth rate and heat stress tolerance were positively correlated with protein titres, while submerged pellet diameter and cell wall integrity were negatively associated with productivity. This study identified potential genetic targets for maximizing protein titres and provided chassis strains with controllable macromorphologies for pilot fermentation studies.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2023)
Article
Chemistry, Physical
Sonia F. G. Santos, Rajesh Reddy Bommareddy, Gary W. Black, Warispreet Singh, Meilan Huang
Summary: The AgcA(P450) enzyme has been found to oxidize bulkier substrates like 4-propylguaiacol more efficiently than smaller substrates like guaiacol, due to its open conformation of the substrate access channel and larger pocket for accommodating bulkier substrates. This understanding of the enzyme's substrate preference can be used for engineering enzymes for the conversion of lignocellulosic biomass.
CATALYSIS SCIENCE & TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Kamran Jawed, Victor Uhunoma Irorere, Rajesh Reddy Bommareddy, Nigel P. Minton, Katalin Kovacs
Summary: The facultative chemolithoautotroph Cupriavidus necator H16 can grow aerobically with organic substrates or H-2 and CO2, and accumulate a large amount of poly (3-hydroxybutyrate) (PHA). This study investigated the ability of this organism to co-utilize volatile fatty acids (VFAs) and CO2 for carbon sources under mixotrophic growth conditions, and monitored PHA production. The results showed that PHA accumulation was significantly higher when cultures were grown mixotrophically with H-2 as an additional energy source. A two-step VFA feeding regime using a VFA mixture derived from an anaerobic digestor (AD) was found to be the most effective method for PHA accumulation.
FERMENTATION-BASEL
(2022)
