Article
Immunology
Carlos Munoz, Josue Gonzalez-Lorca, Mick Parra, Sarita Soto, Natalia Valdes, Ana Maria Sandino, Rodrigo Vargas, Alex Gonzalez, Mario Tello
Summary: The study explored the use of Lactococcus lactis as a type I interferon expression system in Atlantic salmon to stimulate the antiviral immune response, showing that oral administration of L. lactis producing interferon I induces systemic effects, reduces IPNV viral load, and increases Mx and PKR expression.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Katarzyna Kaczmarek, Jerzy Wieckiewicz, Kazimierz Weglarczyk, Maciej Siedlar, Jarek Baran
Summary: This study investigated the use of genetically modified Lactococcus lactis bacteria for local delivery of human soluble TRAIL in CRC treatment. The results showed selective elimination of CRC cells and enhanced anti-tumor activity with oral administration of Metformin.
Article
Biotechnology & Applied Microbiology
Abida Zahirovic, Tina Vida Plavec, Ales Berlec
Summary: Pro-inflammatory cytokines have an important role in colorectal cancer. This study developed bacteria with dual functionality to deliver cytokine-binding proteins to tumors, potentially blocking their tumor-promoting effects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Roslina Jawan, Sahar Abbasiliasi, Shuhaimi Mustafa, Mohammad Rizal Kapri, Murni Halim, Arbakariya B. Ariff
Summary: The study demonstrated that a newly isolated bacteriocin-producing Lactococcus lactis strain has the potential to be applied in the food industry for various purposes, such as starter culture preparation, functional foods, and probiotic products.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Biochemistry & Molecular Biology
Raman Krishnamoorthi, Moovendran Srinivash, Pambayan Ulagan Mahalingam, Balasubramanian Malaikozhundan, Paulraj Suganya, Krishnamoorthy Gurushankar
Summary: The current study aimed to screen bacteriocin producing LAB from different dairy products and evaluate their biological properties. Among the isolated LAB species, one strain (CH3) showed better antimicrobial effects and its purified bacteriocin demonstrated strong antimicrobial and anti-biofilm activity against selected human pathogens. These findings suggest the potential application of bacteriocin in combating antimicrobial-resistant pathogens and preserving food without contamination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Adilene Mendoza-Salazar, Lourdes Santiago-Lopez, Maria J. Torres-Llanez, Adrian Hernandez-Mendoza, Belinda Vallejo-Cordoba, Andrea M. Liceaga, Aaron F. Gonzalez-Cordova
Summary: This study evaluated the antioxidant and ACEI activity of edible insect flours fermented with specific Lactococcus lactis strains. The results showed that fermented grasshopper flours demonstrated higher antioxidant activity and ACEI inhibition compared to mealworm flours. Fermentation increased protein hydrolysis, total polyphenol content, and peptide profile, providing potential bioactive compounds for antioxidant and antihypertensive purposes.
FERMENTATION-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Gabriela N. Tenea
Summary: This study isolated a novel Lactococcus lactis strain from wild Amazonian camu-camu fruits, which contained various functional metabolites with antibacterial capacity. Genome analysis revealed high genetic diversity within the strain. Additionally, the strain was predicted to be non-pathogenic, lacked genes related to the production of biogenic amines and acquired antibiotic resistance genes. The study highlights the potential of this strain for producing bioactive metabolites for agri-food and pharmaceutical industry use.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Nitish Boodhoo, Bahram Shojadoost, Mohammadali Alizadeh, Raveendra R. Kulkarni, Shayan Sharif
Summary: The current study aimed to characterize chicken intestinal immune system cells and assess the immune modulatory action of Clostridium perfringens and Lactococcus lactis on these cells. The results demonstrated varying distribution of immune cells along the avian gut-associated lymphoid tissue (GALT) and showed that both C. perfringens and L. lactis increased the frequency of CD25+ T cells. However, stimulation with C. perfringens led to more cell death and decreased macrophage activation, while stimulation with L. lactis activated T cells and rescued the immune response of these cells. These findings suggest the potential application of L. lactis as a probiotic against C. perfringens infection in chickens.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Nutrition & Dietetics
Chee-Sieng Khor, Ryohei Tsuji, Hai-Yen Lee, Siti-Sarah Nor'e, Norhidayu Sahimin, Adzzie-Shazleen Azman, Vunjia Tiong, Pouya Hasandarvish, Boon-Teong Teoh, Yih-Harng Soh, Jian-Hai Chai, Takeshi Kokubo, Osamu Kanauchi, Naoki Yamamoto, Sazaly AbuBakar
Summary: The study demonstrated that oral intake of Lactococcus lactis strain plasma (LC-Plasma) significantly reduces the cumulative days with dengue fever-like symptoms, including fever, muscle pain, joint pain, and pain behind the eyes. Furthermore, the severity score of symptoms was significantly reduced in the LC-Plasma group according to subgroup analysis.
Article
Microbiology
Magdalena Chmielewska-Jeznach, Kamil Steczkiewicz, Kamil Kobylecki, Jacek K. Bardowski, Agnieszka K. Szczepankowska
Summary: In this study, the functional characterization of a product of an early gene (gp46) from a virulent L. lactis phage, vB_Llc_bIBBF13, was described. The protein GP46(F13) carries a catalytically active RecA-like domain and exhibits helicase activity. It also interacts with host proteins involved in DNA replication.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jihye Baek, Jong-Hwa Kim, Wonyong Kim
Summary: In this study, the effectiveness of L. lactis LB 1022 isolated from natural cheese was evaluated for its anti-allergy and anti-inflammatory properties. The results showed that L. lactis LB 1022 exhibited nitric oxide suppression and reduced levels of IgE, mast cells, eosinophils, and T cell-mediated cytokines in an AD mouse model. Furthermore, L. lactis LB 1022 treatment increased the concentration of short-chain fatty acids. Overall, these findings suggest that L. lactis LB 1022 has potential as a functional food supplement to alleviate AD symptoms.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Behavioral Sciences
Juliana Bernera Ramalho, Cristiano Chiapinotto Spiazzi, Diogo Ferreira Bicca, Jessica Ferreira Rodrigues, Carla Pohl Sehn, Wladimir Padilha da Silva, Francis Weber, Santos Cibin
Summary: This study evaluated the effectiveness of a probiotic intervention using Lactococcus lactis subsp. cremoris LL95 in ameliorating mood disorders in a lipopolysaccharide (LPS)-induced depression-like mouse model. The results showed that LL95 intervention improved LPS-induced depression-like behaviors in mice and modulated oxidative and inflammatory markers in the hippocampus, as well as altered the LAB content of the gut microbiota.
BEHAVIOURAL BRAIN RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Roslina Jawan, Sahar Abbasiliasi, Joo Shun Tan, Murni Halim, Shuhaimi Mustafa, Bin Hao Lee, Jia Sim Kwa, Arbakariya B. Ariff
Summary: The study optimized parameters for the partitioning of bacteriocin-like inhibitory substances (BLIS) from Lactococcus lactis Gh1 using a polyethylene glycol (PEG)/dextran aqueous two-phase system (ATPS). The results showed that specific compositions of PEG and dextran in the ATPS significantly affected the partition coefficient, purification factor, and yield of BLIS, demonstrating the effectiveness of the system in extractive fermentation.
FERMENTATION-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Hanlin Zhang, Meng Dong, Shouli Yuan, Wanzhu Jin
Summary: We constructed a recombinant oral GLP-1 analogue and found that it reduced binding and cleavage by DPP4 in L. lactis. In addition, it improved blood glucose levels and reduced inflammation in db/db mice.
BIOTECHNOLOGY LETTERS
(2022)
Article
Agriculture, Dairy & Animal Science
Tien Dat Nguyen, Honghui Li, Yiquan Zhuang, Bowei Chen, Keiji Kinoshita, Muhammad Ameen Jamal, Kaixiang Xu, Jianxiong Guo, Deling Jiao, Kumiko Tanabe, Yunfang Wei, Zhuo Li, Wenming Cheng, Yubo Qing, Hong-Ye Zhao, Hong-Jiang Wei
Summary: This study successfully established an Asian elephant's fibroblast cell lines and performed interspecies somatic cell nuclear transfer with porcine oocytes. The reconstructed embryos showed in vitro development but failed to develop in vivo. Further investigation is needed to understand the reasons behind the in vivo developmental failure.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Joanna Zycka-Krzesinska, Joanna Boguslawska, Tamara Aleksandrzak-Piekarczyk, Jakub Jopek, Jacek K. Bardowski
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2015)
Article
Genetics & Heredity
Joanna Boguslawska, Elzbieta Sokol, Beata Rybicka, Alicja Czubaty, Katarzyna Rodzik, Agnieszka Piekielko-Witkowska
Article
Urology & Nephrology
Joanna Boguslawska, Hanna Kedzierska, Piotr Poplawski, Beata Rybicka, Zbigniew Tanski, Agnieszka Piekielko-Witkowska
JOURNAL OF UROLOGY
(2016)
Article
Cell Biology
Piotr Poplawski, Beata Rybicka, Joanna Boguslawska, Katarzyna Rodzik, Theo J. Visser, Alicja Nauman, Agnieszka Piekielko-Witkowska
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2017)
Article
Oncology
Joanna Boguslawska, Katarzyna Rodzik, Piotr Poplawski, Hanna Kedzierska, Beata Rybicka, Elzbieta Sokol, Zbigniew Tanski, Agnieszka Piekielko-Witkowska
Article
Oncology
Elibieta Sokol, Hanna Kedzierska, Alicja Czubaty, Beata Rybicka, Katarzyna Rodzik, Zbigniew Tanski, Joanna Boguslawska, Agnieszka Piekielko-Witkowska
EXPERIMENTAL CELL RESEARCH
(2018)
Article
Oncology
Joanna Boguslawska, Piotr Poplawski, Saleh Alseekh, Marta Koblowska, Roksana Iwanicka-Nowicka, Beata Rybicka, Hanna Kedzierska, Katarzyna Gluchowska, Karolina Hanusek, Zbigniew Tanski, Alisdair R. Fernie, Agnieszka Piekielko-Witkowska
Review
Cell Biology
Joanna Boguslawska, Piotr Kryst, Slawomir Poletajew, Agnieszka Piekielko-Witkowska
Correction
Biochemistry & Molecular Biology
Hanna Kedzierska, Piotr Poplawski, Grazyna Hoser, Beata Rybicka, Katarzyna Rodzik, Elzbieta Sokol, Joanna Boguslawska, Zbigniew Tanski, Anna Fogtman, Marta Koblowska, Agnieszka Piekielko-Witkowska
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Piotr Poplawski, Joanna Boguslawska, Karolina Hanusek, Agnieszka Piekielko-Witkowska
Summary: Renal cell cancer, especially clear cell renal cell carcinoma (ccRCC), is known for its high aggressiveness and poor prognosis for patients. The nucleoli, as crucial substructures of nuclei, play a significant role in controlling cellular functioning and homeostasis through multiple non-coding RNAs, such as snoRNAs. Dysregulation of snoRNAs, including SNHG1, SNHG4, and SNHG12, in ccRCC tumors contributes to the aberrant expression of oncogenes and tumor suppressors, directly impacting ccRCC development and progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Karolina Hanusek, Slawomir Poletajew, Piotr Kryst, Agnieszka Piekielko-Witkowska, Joanna Boguslawska
Summary: piRNAs are a class of small non-coding RNAs that regulate transposon and gene expression through mechanisms such as DNA methylation, histone modifications, and mRNA degradation. Recent studies suggest that piRNAs could serve as potential biomarkers for early cancer detection and therapeutic interventions, playing a role in the progression of prostate cancer.
Review
Pediatrics
Artur Niedzielski, Lechoslaw Pawel Chmielik, Grazyna Mielnik-Niedzielska, Anna Kasprzyk, Joanna Boguslawska
Summary: Adenoids, part of Waldeyer's ring, are lymphoid tissues located at the junction of the roof and posterior wall of the nasopharynx. They play a crucial role in immune system development and serve as the first line of defense against respiratory and digestive antigens. The causes of adenoid hypertrophy are not fully understood, but they are likely associated with immune reactions, infections, environmental exposures, and hormonal or genetic factors. This review aims to summarize current knowledge about adenoid hypertrophy in children and its associated diseases. Adenoid hypertrophy has various clinical manifestations in pediatric patients and is often accompanied by comorbidities.
BMJ PAEDIATRICS OPEN
(2023)
Review
Medicine, Research & Experimental
Elzbieta Sokol, Joanna Boguslawska, Agnieszka Piekielko-Witkowska
POSTEPY HIGIENY I MEDYCYNY DOSWIADCZALNEJ
(2017)
Article
Biochemistry & Molecular Biology
Piotr Poplawski, Takayuki Tohge, Joanna Boguslawska, Beata Rybicka, Zbigniew Tanski, Victor Trevino, Alisdair R. Fernie, Agnieszka Piekielko-Witkowska
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2017)
Article
Biochemistry & Molecular Biology
Hanna Kedzierska, Piotr Poplawski, Grazyna Hoser, Beata Rybicka, Katarzyna Rodzik, Elzbieta Sokol, Joanna Boguslawska, Zbigniew Tanski, Anna Fogtman, Marta Koblowska, Agnieszka Piekielko-Witkowska
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2016)