Article
Food Science & Technology
Ana Rita Barata, Barbara Nunes, Ricardo Oliveira, Hugo Guedes, Carina Almeida, Maria Jose Saavedra, Gabriela Jorge da Silva, Goncalo Almeida
Summary: The growing belief in the health benefits of natural products, such as raw milk, has led to behaviors that can contribute to food safety issues and increased healthcare costs. This study investigated the occurrence and seasonality of Campylobacter spp. in raw cow's milk and the potential routes of contamination in dairy farm environments. The findings highlight the potential risk of campylobacteriosis associated with consuming raw milk and emphasize the importance of routine surveillance in dairy farms for zoonotic pathogens.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Nadine A. Vogt, David L. Pearl, Eduardo N. Taboada, Steven K. Mutschall, Kristin J. Bondo, Claire M. Jardine
Summary: Campylobacter is a common cause of foodborne illness in humans globally, with recent research suggesting that wildlife may play a role in transmission to humans. A study in southern Ontario investigated the occurrence of Campylobacter jejuni in raccoons, finding that climatic factors were associated with the presence of the bacteria in raccoons, but the effects varied by location and year. Further research is needed to understand the transmission and movement of Campylobacter within the ecosystem.
ZOONOSES AND PUBLIC HEALTH
(2021)
Article
Multidisciplinary Sciences
Yuki Otsuka, Hideharu Hagiya, Misa Takahashi, Shinnosuke Fukushima, Ruri Maeda, Naruhiko Sunada, Haruto Yamada, Masayuki Kishida, Koji Fujita, Fumio Otsuka
Summary: In this retrospective, multicenter study, the clinical characteristics of Campylobacter bacteremia in western Japan were investigated. The study revealed that nearly half of the patients presented with fever as the main symptom, rather than gastrointestinal symptoms.
SCIENTIFIC REPORTS
(2023)
Article
Environmental Sciences
Shuxin Zhang, Jiahua Shi, Xuan Li, Lachlan Coin, Jake W. O'Brien, Muttucumaru Sivakumar, Faisal Hai, Guangming Jiang
Summary: This study developed a triplex qPCR assay for the detection and quantification of Campylobacter species in wastewater samples, specifically targeting C. jejuni and C. coli. The assay demonstrated high sensitivity and reliability, and can be used for long-term monitoring of Campylobacter prevalence in communities and surrounding environments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Anna-Delia Knipper, Narges Ghoreishi, Tasja Crease
Summary: This systematic review and meta-analysis summarizes data on the prevalence and concentration of Campylobacter in faeces of dairy cows. The results show significant differences in prevalence between studies and the impact of faecal collection methods and Campylobacter species on prevalence. However, there are important data gaps and limitations in current studies, highlighting the need for further research.
Article
Food Science & Technology
Anna-Delia Knipper, Steven Goehlich, Kerstin Stingl, Narges Ghoreishi, Carola Fischer-Tenhagen, Niels Bandick, Bernd-Alois Tenhagen, Tasja Crease
Summary: This study investigated the variation in the prevalence and concentration of Campylobacter spp. in cows' milk, feces, and the farm environment over one year at a small German dairy farm. The highest prevalence of Campylobacter spp. was found in feces (77.1%), while it was completely absent in milking equipment and low in raw milk (0.4%). The study highlights the persistence of Campylobacter spp. in individual cows' intestines and the farm environment for at least one year, as well as the possibility of fecal cross-contamination of teats even when raw milk contamination is rare.
Article
Agriculture, Dairy & Animal Science
Gaia Casalino, Francesco D'Amico, Francesca Rita Dinardo, Giancarlo Bozzo, Valeria Napoletano, Antonio Camarda, Antonella Bove, Roberto Lombardi, Francesco Paolo D'Onghia, Elena Circella
Summary: This study investigated the presence of Campylobacter (C. jejuni and C. coli) in wild birds housed at a wildlife rescue centre. It was found that 24.88% of the birds carried Campylobacter, with higher rates of infection in omnivorous species and indoor aviaries. Interestingly, Campylobacter was not detected in species with body temperatures below 40°C or above 42.2°C. Antibiotic resistance, including multi-drug resistance, was also identified. These findings underscore the importance of increasing biosecurity measures at wildlife rescue centres to reduce health risks to staff involved in wildlife management.
Article
Engineering, Environmental
Shuxin Zhang, Jiahua Shi, Elipsha Sharma, Xuan Li, Shuhong Gao, Xu Zhou, Jake O'Brien, Lachlan Coin, Yanchen Liu, Muttucumaru Sivakumar, Faisal Hai, Guangming Jiang
Summary: This study investigated the recovery of Campylobacter spp. from wastewater and their decay under different simulated sewer reactor conditions. The results showed that the recovery of Campylobacter spp. varied with their concentrations in wastewater and the detection limit of quantification methods. The concentration reduction of Campylobacter spp. in sewers followed a two-phase reduction model, with the faster reduction in the first phase mainly due to partitioning onto sewer biofilms. The total decay of Campylobacter spp. varied in different types of sewer reactors.
Article
Public, Environmental & Occupational Health
Jose A. Rodrigues, Wonhee Cha, Rebekah E. Mosci, Sanjana Mukherjee, Duane W. Newton, Paul Lephart, Hossein Salimnia, Walid Khalife, James T. Rudrik, Shannon D. Manning
Summary: In a four-year active surveillance study at four metropolitan hospitals in Michigan, an increasing trend in Campylobacter jejuni infections was observed. Analysis of 214 C. jejuni isolates revealed widespread antibiotic resistance, with differences in infection rates by hospital type and patient characteristics associated with disease severity.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Veterinary Sciences
Debora Brito Goulart, Qijing Zhang, Orhan Sahin
Summary: Human enterocolitis is commonly caused by the Gram-negative bacterium Campylobacter jejuni. The emergence of fluoroquinolone-resistant (FQ-R) Campylobacter has increased in poultry and cattle, which are important reservoirs of the bacteria. In this study, it was found that FQ-R Campylobacter strains from cattle had slightly higher fitness advantage over susceptible strains, but the emergence of FQ-R mutants was primarily influenced by bacterial cell density and antibiotic concentration. These findings provide explanations for the prevalence of FQ-R Campylobacter in cattle production and the limited development of FQ-R Campylobacter in response to treatment in cattle intestines.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Food Science & Technology
Xiaohong Cheng, Weixing Liu, Zhuangzhuang Wang, Rongxue Yang, Lejun Yu, Qingbao Du, Anshan Ge, Chenguang Liu, Zhe Chi
Summary: The study developed an improved biosensor for the rapid detection of Campylobacter jejuni in food and animal feces. The biosensor demonstrated high sensitivity and a low detection limit, with the ability to detect 101 CFU/mL within 60 minutes. Microfluidics was used to eliminate interferences and enhance the accuracy of the detection.
Article
Multidisciplinary Sciences
Medelin Ocejo, Beatriz Oporto, Jose Luis Lavin, Ana Hurtado
Summary: A long-term surveillance study on dairy cattle farms revealed that C. jejuni was more prevalent than C. coli, and resistance rates were higher in calves. Some genotypes persisted throughout the study while others were sporadically detected. Acquisition of extracellular genes and intracellular mutational events were identified as the processes that led to the emergence and spread of resistant genotypes. Oxford Nanopore Technologies sequencing helped decipher the complex molecular epidemiology of resistant Campylobacter dissemination.
SCIENTIFIC REPORTS
(2023)
Review
Chemistry, Multidisciplinary
Nikolaos D. Andritsos, Nikolaos Tzimotoudis, Marios Mataragas
Summary: It is well-established that raw or incompletely thermally processed poultry meat is a major source of strong-evidence foodborne outbreaks of human campylobacteriosis. The dominant species of Campylobacter in chicken meat samples has traditionally been C. jejuni, but recently, C. coli has been increasingly recovered from chicken meat to the extent that it often comprises the majority of identified campylobacters. This review provides a detailed analysis of the changing prevalence of C. jejuni and C. coli in chicken meat, as well as the factors influencing their distribution.
APPLIED SCIENCES-BASEL
(2023)
Article
Microbiology
Malte Herold, Louise Hock, Christian Penny, Cecile Walczak, Fatu Djabi, Henry-Michel Cauchie, Catherine Ragimbeau
Summary: As the leading cause of human gastroenteritis, Campylobacter must be monitored extensively using a One Health approach. Wild birds are believed to play a role in spreading recurrent C. jejuni genotypes across countries. To address the challenge of genomic diversity, a filtration method was used to obtain isolates and genotypes were recovered from metagenomic sequencing data. A computational workflow was developed to genotype mixed strains. This study provides a foundation for future research on C. jejuni diversity in environmental and clinical settings.
Article
Infectious Diseases
Lucia Varela-Castro, Marta Barral, Maria Cruz Arnal, Daniel Fernandez de Luco, Christian Gortazar, Joseba M. Garrido, Iker A. Sevilla
Summary: This study found that there is a high diversity of non-tuberculous mycobacteria (NTM) circulating among wildlife and livestock in Spain. Wild boar and M. avium seem to play a significant role in this epidemiological scenario.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Microbiology
Robert William O'Hara, Peter J. Jenks, Matthew Emery, Mathew Upton
JOURNAL OF MEDICAL MICROBIOLOGY
(2019)
Article
Multidisciplinary Sciences
Sih-Jing Liao, Jonathan Marshall, Martin L. Hazelton, Nigel P. French
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2019)
Article
Microbiology
Abdu Aldarhami, Arif Felek, Vikram Sharma, Mathew Upton
JOURNAL OF MEDICAL MICROBIOLOGY
(2020)
Letter
Infectious Diseases
Sean D. W. Comber, Mathew Upton, Shaun Lewin, Neil Powell, Thomas H. Hutchinson
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Article
Multidisciplinary Sciences
Katharine Hammond, Helen Lewis, Samantha Halliwell, Florie Desriac, Brunello Nardone, Jascindra Ravi, Bart W. Hoogenboom, Mathew Upton, Jeremy P. Derrick, Maxim G. Ryadnov
Article
Food Science & Technology
Yinhua Deng, Min Jiang, Patrick S. L. Kwan, Chao Yang, Qiongcheng Chen, Yiman Lin, Yaqun Qiu, Yinghui Li, Xiaolu Shi, Liqiang Li, Yujun Cui, Qun Sun, Qinghua Hu
Summary: This study utilized whole-genome sequencing (WGS) to conduct outbreak detection of Salmonella enterica serotype Enteritidis, demonstrating the superior discriminatory power and accuracy of WGS in pragmatic food source tracing. The proposed integrated WGS framework in China for Salmonella Enteritidis has the potential to serve as a paradigm for outbreak detection and source tracing of Salmonella and other foodborne pathogens.
FOODBORNE PATHOGENS AND DISEASE
(2021)
Article
Microbiology
Shuang Wu, Xiaolu Shi, Qiongcheng Chen, Yixiang Jiang, Le Zuo, Lei Wang, Min Jiang, Yiman Lin, Shisong Fang, Bo Peng, Weihua Wu, Hui Liu, Renli Zhang, Patrick S. L. Kwan, Qinghua Hu
Summary: This study compared the specificity and sensitivity of CPA and RT-qPCR kits for SARS-CoV-2 detection, providing a reference for clinical laboratories and informing testing protocols during the rapidly evolving COVID-19 pandemic. The CPA kit showed 100% sensitivity, specificity, PPV/NPV, while some RT-qPCR kits demonstrated good agreement with clinical diagnostic reports and good reproducibility. Differences between all kits were statistically significant.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2021)
Article
Microbiology
James Butler, Sean D. Kelly, Katie J. Muddiman, Alexandros Besinis, Mathew Upton
Summary: This study reports the characterization of a strain of C. pauculus isolated from a hospital sink trap and assesses its potential risk to human health. The findings show that the strain is multidrug-resistant and capable of biofilm formation.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Microbiology
Matthew J. Koch, Poppy J. Hesketh-Best, Gary Smerdon, Philip J. Warburton, Kerry Howell, Mathew Upton
Summary: Through culture-based microbial analysis of deep-sea sponges, this study discovered novel antimicrobial candidates and demonstrated antimicrobial activity, showcasing the potential for screening novel antimicrobial compounds.
Article
Microbiology
Min Jiang, Chao Yang, Patrick S. L. Kwan, Liping Zhang, Hang Fan, Yujuan Jin, Lifang Sun, Hongyu Chen, Baisheng Li, Qiuxia Chen, Yarong Wu, Yan Guo, Yuanguo Shi, Min Liao, Xiaolu Shi, Jianping Liu, Lijuan Jiang, Rui Cai, Yinhua Deng, Qun Sun, Ruifu Yang, Qiaoli Zhang, Yujun Cui, Qinghua Hu
Summary: On September 21, 2019, a large cluster of suspected gastroenteritis involving primarily children was reported in Shenzhen and Dongguan, China. Prompt joint outbreak response and multidisciplinary cooperation led to the identification of eggs served as sandwiches as the source of the outbreak. Whole-genome analyses and genomic source tracing revealed the transmission dynamics of the Salmonella Enteritidis clone through a multi-provincial egg distribution network. The efficient coordination of approaches in this outbreak was unprecedented in China and successfully intervened in a large cross-jurisdiction Salmonella outbreak.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Infectious Diseases
Neil Powell, Jennie Stephens, Declan Kohl, Rhys Owens, Shadia Ahmed, Crispin Musicha, Mathew Upton, Bridie Kent, Sarah Tonkin-Crine, Jonathan Sandoe
Summary: This systematic review examined the effectiveness and safety of nonallergist health care worker delivery of penicillin allergy delabeling. The results showed that delabeling by nonallergists is effective and safe, with different testing methods leading to varying proportions of patients being delabeled.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Hematology
Susy Kirwan, Patrick S. L. Kwan, Ronald So, Alice Tuinukuafe
Summary: A fully closed manufacturing process for serum eye drops was implemented at New Zealand Blood Service, reducing production time by up to 45% and eliminating bacterial contamination risks. This dockable system enhances patient safety, reduces manufacturing time and cost.
Article
Immunology
Min Jiang, Feng Zhu, Chao Yang, Yinhua Deng, Patrick S. L. Kwan, Yinghui Li, Yiman Lin, Yaqun Qiu, Xiaolu Shi, Hui Chen, Yujun Cui, Qinghua Hu
EMERGING INFECTIOUS DISEASES
(2020)
Article
Genetics & Heredity
Nicole E. Wheeler, Timothy Blackmore, Angela D. Reynods, Anne C. Midwinter, Jonathan Marshall, Nigel P. French, Matthew S. Savoian, Paul P. Gardner, Patrick J. Biggs
MICROBIAL GENOMICS
(2019)
Article
Immunology
A. Springer Browne, Patrick J. Biggs, David A. Wilkinson, Adrian L. Cookson, Anne C. Midwinter, Samuel J. Bloomfield, C. Reed Hranac, Lynn E. Rogers, Jonathan C. Marshall, Jackie Benschop, Helen Withers, Steve Hathaway, Tessy George, Patricia Jaros, Hamid Irshad, Yang Fong, Muriel Dufour, Naveena Karki, Taylor Winkleman, Nigel P. French
EMERGING INFECTIOUS DISEASES
(2019)