Article
Multidisciplinary Sciences
Daniel Ellis, Julia Lederhofer, Oliver J. Acton, Yaroslav Tsybovsky, Sally Kephart, Christina Yap, Rebecca A. Gillespie, Adrian Creanga, Audrey Olshefsky, Tyler Stephens, Deleah Pettie, Michael Murphy, Claire Sydeman, Maggie Ahlrichs, Sidney Chan, Andrew J. Borst, Young-Jun Park, Kelly K. Lee, Barney S. Graham, David Veesler, Neil P. King, Masaru Kanekiyo
Summary: This study reveals the conformational stability of influenza virus neuraminidase (NA) and demonstrates how recombinant NA antigens can be strengthened through structure-based design.
NATURE COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Jeremy C. Jones, Hui-Ling Yen, Peter Adams, Kimberly Armstrong, Elena A. Govorkova
Summary: Effective antivirals play a crucial role in the early phase of an influenza pandemic, before vaccines are available. Currently, two classes of antiviral drugs are approved for influenza treatment, but resistance has been observed. Therefore, it is important to develop novel antiviral options that target both the virus and the host. This review explores the drawbacks of current antivirals and ongoing research on new agents or combination therapies to address these limitations.
ANTIVIRAL RESEARCH
(2023)
Article
Chemistry, Medicinal
Avishak Sarker, Zichen Gu, Lu Mao, Yongzhuang Ge, Duoduo Hou, Jieyu Fang, Zhanyong Wei, Zhenya Wang
Summary: Influenza is a century-old disease that poses challenges to humans due to its mutating nature, seasonal epidemics, and occasional pandemics. The limited effectiveness of strain-specific vaccines and the growing drug resistance to anti-influenza drugs call for the development of novel drugs with broad reactivity, higher bioavailability, easier administration, and fewer side effects.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Veterinary Sciences
Amanda L. Skarlupka, Ted M. Ross
Summary: Research has shown that raw animal sera can inhibit a range of influenza virus neuraminidase (NA), but the breadth and effectiveness of inhibition depend on the source of sera. Despite different sources of sera exhibiting varying levels of inhibition against different influenza viruses, further studies are needed to ensure consistency of scientific methods in order to support the development of broadly protective influenza virus vaccines.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Andrei A. Ivashchenko, Jeremy C. Jones, Dmitry O. Shkil, Yan A. Ivanenkov, Philippe Noriel Q. Pascua, Melissa K. Penaflor, Ruben N. Karapetian, Elena A. Govorkova, Alexandre V. Ivachtchenko
Summary: In this study, the efficacy of a new orally-dosed neuraminidase inhibitor (NAI) AV5080 against different subtypes of influenza viruses was examined. AV5080 showed superior in vitro efficacy compared to currently approved NAIs, oseltamivir and zanamivir. However, it exhibited reduced inhibition against certain viral variants, such as NA-E119G and NA-R292K. These findings suggest that AV5080 is a promising orally-dosed NAI.
ANTIVIRAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Ahmed Kandeil, Christopher Patton, Jeremy C. Jones, Trushar Jeevan, Walter N. Harrington, Sanja Trifkovic, Jon P. Seiler, Thomas Fabrizio, Karlie Woodard, Jasmine C. Turner, Jeri-Carol Crumpton, Lance Miller, Adam Rubrum, Jennifer DeBeauchamp, Charles J. Russell, Elena A. Govorkova, Peter Vogel, Mia Kim-Torchetti, Yohannes Berhane, David Stallknecht, Rebecca Poulson, Lisa Kercher, Richard J. Webby
Summary: In 2021, highly pathogenic avian influenza A(H5N1) viruses of clade 2.3.4.4b spread rapidly among wild birds and domestic poultry across Asia, Europe, and Africa, and reached North America. The movement of clade 2.3.4.4b was followed by reassortment with viruses circulating in wild birds in North America, resulting in the emergence of genetically and phenotypically diverse A(H5N1) viruses that cause severe disease with neurological involvement in mammals. The ability of the current A(H5N1) 2.3.4.4b virus lineage to reassort and target the central nervous system highlights the need for coordinated planning to control the spread and evolution of the virus and to mitigate the impact of a potential influenza pandemic.
NATURE COMMUNICATIONS
(2023)
Article
Infectious Diseases
Yanna Guo, Pingyun Ding, Yinjing Li, Yaping Zhang, Yiqing Zheng, Mengqi Yu, Yasuo Suzuki, Haitao Zhang, Jihui Ping
Summary: This study systematically investigated three strains of H10N3 avian influenza virus isolated from live poultry markets and found that these viruses were highly pathogenic to mice, replicated efficiently in mouse nasal turbinate and lungs as well as in A549 cells and chicken embryos, and possessed key molecular markers associated with mammalian adaptation.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Mher Matevosyan, Vardan Harutyunyan, Narek Abelyan, Hamlet Khachatryan, Irina Tirosyan, Yeva Gabrielyan, Valter Sahakyan, Smbat Gevorgyan, Vahram Arakelov, Grigor Arakelov, Hovakim Zakaryan
Summary: The influenza virus poses a significant public health challenge due to its high morbidity and mortality rates, as well as the emergence of drug-resistant strains. In this study, a deep reinforcement learning algorithm was used to design new chemical entities that have better binding energy than oseltamivir, an antiviral drug commonly used as a first-line defense. These potential compounds showed promising results in molecular dynamics simulations and demonstrated better binding affinity to certain neuraminidases of the influenza virus than oseltamivir. The findings suggest the possibility of finding novel scaffolds with improved binding and antiviral properties. Further synthesis and testing are recommended for these compounds as potential antiviral candidates.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biology
Logan Kaler, Ethan Iverson, Shahed Bader, Daniel Song, Margaret A. Scull, Gregg A. Duncan
Summary: This study utilized fluorescence video microscopy to investigate the movement of Influenza A virus (IAV) in mucus. The results showed that the mesh structure of the gel network significantly influenced the virus's mobility and the IAV-mucus binding was found to be weak. These findings provide important insights into how the adhesive and physical barrier properties of mucus impact the dissemination of IAV within the lung microenvironment.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Beryl Mazel-Sanchez, Justyna Iwaszkiewicz, Joao P. P. Bonifacio, Filo Silva, Chengyue Niu, Shirin Strohmeier, Davide Eletto, Florian Krammer, Gene Tan, Vincent Zoete, Benjamin G. Hale, Mirco Schmolke
Summary: The excessive production of viral glycoproteins during infections can put a significant stress on the host cell's endoplasmic reticulum protein folding machinery. However, viruses have developed mechanisms to optimize this stress for their own replication by balancing folding capacity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Manu Kumar, Sang-Min Chung, Ganuskh Enkhtaivan, Rahul V. Patel, Han-Seung Shin, Bhupendra M. Mistry
Summary: Newly synthesized substituted benzothiazole based berberine derivatives demonstrated interesting anti-influenza virus activity, with BBD7 showing potent neuraminidase activity. Molecular docking analysis suggests that the antiviral mechanisms of these compounds may be similar to oseltamivir through interaction with residues of neuraminidase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Svetlana Svyatchenko, Natalia Goncharova, Vasiliy Y. Marchenko, Natalia P. Kolosova, Alexander N. Shvalov, Valentina L. Kovrizhkina, Alexander G. Durymanov, Galina S. Onkhonova, Tatyana Tregubchak, Ivan M. Susloparov, Andrey S. Gudymo, Tatyana N. Ilyicheva, Alexander B. Ryzhikov
Summary: This study assessed antiviral susceptibility of influenza A(H5N8) viruses in Russia, finding that only one strain had amino acid substitution related to adamantane resistance, and another strain had a substitution associated with reduced inhibition by oseltamivir. Global analysis revealed a small percentage of H5N8 viruses showing resistance to antiviral agents.
ANTIVIRAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jiwei Zhang, Chuanfeng Liu, Ruifang Jia, Xujie Zhang, Jian Zhang, Chiara Bertagnin, Anna Bonomini, Laura Guizzo, Yuanmin Jiang, Huinan Jia, Shuzhen Jia, Xiuli Ma, Arianna Loregian, Bing Huang, Peng Zhan, Xinyong Liu
Summary: Introducing structurally diverse benzyl side chains at the C5-NH2 position of oseltamivir improves its binding affinity with neuraminidase and anti-influenza activity. We designed and synthesized novel oseltamivir derivatives with different N-heterocycle substituents that induce opening of the 150-loop of group-2 neuraminidases. Compound 6k showed broad-spectrum activity against wild-type neuraminidases and enhanced activity against H3N2 compared to oseltamivir carboxylate and zanamivir.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Immunology
Miao He, Zhihui Ren, Mohsan Ullah Goraya, Yinhong Lin, Jinghan Ye, Rui Li, Jianping Dai
Summary: Apigetrin, an active compound derived from Ixeris sonchifolia Hance, inhibits influenza A virus (IAV) infection by regulating TLR4 and autophagy signaling pathways. It reduces oxidative stress and cytokine storm induced by IAV.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Infectious Diseases
Yifei Xu, Kuiama Lewandowski, Louise O. Downs, James Kavanagh, Thomas Hender Supsup, Sheila Lumley, Katie Jeffery, Dona Foster, Nicholas D. Sanderson, Ali Vaughan, Marcus Morgan, Richard Vipond Supsup, Miles Carroll Supsup, Timothy Peto, Derrick Crook, A. Sarah Walker, Philippa C. Matthews, Steven T. Pullan
Summary: Nanopore metagenomic sequencing showed high sensitivity and specificity in detecting influenza A viruses, enabling the detection of drug resistance and different subtypes of influenza viruses, providing timely insights for antimicrobial stewardship and vaccine design. This sequencing approach also has the potential to investigate and manage nosocomial outbreaks effectively.
Article
Microbiology
Daisuke Tamura, Margaret Okomo-Adhiambo, Vasiliy P. Mishin, Zhu Guo, Xiyan Xu, Julie Villanueva, Alicia M. Fry, James Stevens, Larisa V. Gubareva
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2015)
Article
Immunology
Daisuke Tamura, Roberta L. DeBiasi, Margaret Okomo-Adhiambo, Vasiliy P. Mishin, Angela P. Campbell, Brett Loechelt, Bernhard L. Wiedermann, Alicia M. Fry, Larisa V. Gubareva
JOURNAL OF INFECTIOUS DISEASES
(2015)
Article
Pharmacology & Pharmacy
M. Okomo-Adhiambo, V. P. Mishin, K. Sleeman, E. Saguar, H. Guevara, E. Reisdorf, R. H. Griesser, K. J. Spackman, M. Mendenhall, M. P. Carlos, B. Healey, K. St George, J. Laplante, T. Aden, S. Chester, X. Xu, L. V. Gubareva
ANTIVIRAL RESEARCH
(2016)
Article
Agriculture, Dairy & Animal Science
M. Okomo-Adhiambo, A. Rink, W. M. Rauw, L. Gomez-Raya
Article
Microbiology
Daisuke Tamura, Ha T. Nguyen, Katrina Sleeman, Marnie Levine, Vasiliy P. Mishin, Hua Yang, Zhu Guo, Margaret Okomo-Adhiambo, Xiyan Xu, James Stevens, Larisa V. Gubareva
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2013)
Review
Infectious Diseases
Margaret Okomo-Adhiambo, Tiffany G. Sheu, Larisa V. Gubareva
INFLUENZA AND OTHER RESPIRATORY VIRUSES
(2013)
Article
Infectious Diseases
Margaret Okomo-Adhiambo, Ha T. Nguyen, Anwar Abd Elal, Katrina Sleeman, Alicia M. Fry, Larisa V. Gubareva
INFLUENZA AND OTHER RESPIRATORY VIRUSES
(2014)
Correction
Virology
Margaret Okomo-Adhiambo, Katrina Sleeman, Kristina Ballenger, Ha T. Nguyen, Vasiliy P. Mishin, Tiffany G. Sheu, James Smagala, Yan Li, Alexander I. Klimov, Larisa V. Gubareva
Article
Genetics & Heredity
Sean A. Buono, Reagan J. Kelly, Nadav Topaz, Adam C. Retchless, Hideky Silva, Alexander Chen, Edward Ramos, Gregory Doho, Agha Nabeel Khan, Margaret A. Okomo-Adhiambo, Fang Hu, Daya Marasini, Xin Wang
FRONTIERS IN GENETICS
(2020)
Article
Microbiology
Darlene D. Wagner, Rachel L. Marine, Edward Ramos, Terry Fei Fan Ng, Christina J. Castro, Margaret Okomo-Adhiambo, Krysten Harvey, Gregory Doho, Reagan Kelly, Yatish Jain, Roman L. Tatusov, Hideky Silva, Paul A. Rota, Agha N. Khan, M. Steven Oberste
Summary: VPipe is an automated bioinformatics pipeline for analyzing NGS data and performing whole-genome assembly and species identification of viral sequences. It offers a user-friendly interface with advanced search capabilities for reviewing results. Additionally, VPipe provides a centralized system for storing and analyzing NGS data, expediting public health responses to viral outbreaks and pathogen surveillance.
MICROBIOLOGY SPECTRUM
(2022)
Proceedings Paper
Mathematical & Computational Biology
Margaret Okomo-Adhiambo, Edward Ramos, Reagan Kelly, Yatish Jain, Roman Tatusov, Anna Montmayeur, Gregory Doho, Rachel L. Marine, Terry Fei Fan Ng, Adam Retchless, Steve Oberste, Paul Rota, Xin Wang, Agha N. Khan
ACM-BCB' 2017: PROCEEDINGS OF THE 8TH ACM INTERNATIONAL CONFERENCE ON BIOINFORMATICS, COMPUTATIONAL BIOLOGY,AND HEALTH INFORMATICS
(2017)
Article
Immunology
Aaron D. Storms, Larisa V. Gubareva, Su Su, John T. Wheeling, Margaret Okomo-Adhiambo, Chao-Yang Pan, Erik Reisdorf, Kirsten St George, Robert Myers, Jason T. Wotton, Sara Robinson, Brandon Leader, Martha Thompson, Marjorie Shannon, Alexander Klimov, Alicia M. Fry
EMERGING INFECTIOUS DISEASES
(2012)
Article
Immunology
Samuel B. Graitcer, Larisa Gubareva, Laurie Kamimoto, Saumil Doshi, Meredith Vandermeer, Janice Louie, Christine Waters, Zack Moore, Katrina Sleeman, Margaret Okomo-Adhiambo, Steven A. Marshall, Kirsten St. George, Chao-Yang Pan, Jennifer M. LaPlante, Alexander Klimov, Alicia M. Fry
EMERGING INFECTIOUS DISEASES
(2011)
Article
Immunology
Margaret Okomo-Adhiambo, Alicia M. Fry, Su Su, Ha T. Nguyen, Anwar Abd Elal, Elizabeth Negron, Julie Hand, Rebecca J. Garten, John Barnes, Xu Xiyan, Julie M. Villanueva, Larisa V. Gubareva
EMERGING INFECTIOUS DISEASES
(2015)