Article
Virology
Liliane Monteiro de Morais, Thiago Santos Chaves, Marco Alberto Medeiros, Kaique Alves Brayner Pereira, Patricia Barbosa Jurgilas, Sheila Maria Barbosa de Lima, Sotiris Missailidis, Ana Maria Bispo de Filippis
Summary: The study aims to select specific aptamers for the composition of specific diagnostics, and four Zika virus-specific aptamers are selected. The selected aptamers can specifically bind to Zika virus and distinguish it from Dengue virus.
Article
Pharmacology & Pharmacy
Keigo Yato, Mami Matsuda, Noriyuki Watanabe, Koichi Watashi, Hideki Aizaki, Takanobu Kato, Koji Tamura, Takaji Wakita, Masamichi Muramatsu, Ryosuke Suzuki
Summary: Direct-acting antivirals (DAAs) have been used for the treatment of hepatitis C virus (HCV), but a vaccine for preventing HCV infection is still unavailable. In this study, a Japanese encephalitis virus (JEV) subviral particle (SVP)-based vaccine was utilized as a platform for DNA immunization, and new positions for inserting HCV epitopes were identified. The results showed that the modified vaccine induced higher levels of neutralizing antibodies against HCV and had minimal impact on neutralization activity against JEV.
ANTIVIRAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Johnathan D. Guest, Ruixue Wang, Khadija H. Elkholy, Andrezza Chagas, Kinlin L. Chao, Thomas E. Cleveland, Young Chang Kim, Zhen-Yong Keck, Alexander Marin, Abdul S. Yunus, Roy A. Mariuzza, Alexander K. Andrianov, Eric A. Toth, Steven K. H. Foung, Brian G. Pierce, Thomas R. Fuerst
Summary: The variability of HCV poses a challenge for vaccine development, but a soluble form of E1E2 has been designed to elicit neutralizing antibodies in vivo and potentially optimize E1E2-based antigen production for future studies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Qing Zhou, Yuhao Yang, Zhanxue Xu, Kai Deng, Zhenzhen Zhang, Jiawei Hao, Ni Li, Yanling Wang, Ziwen Wang, Haihang Chen, Yang Yang, Fei Xiao, Xiaohong Zhang, Song Gao, Yi-Ping Li
Summary: ATAD1 plays a crucial role in maintaining mitochondrial homeostasis and combating viral infection. HCV infection induces mitochondrial fragmentation and enhances the pro-infection effect of ATAD1 knockout. ATAD1 interacts with HCV NS5B protein and promotes its degradation, thereby exerting its antiviral function.
Review
Microbiology
Anshuman Das, Efrain E. Rivera-Serrano, Xin Yin, Christopher M. Walker, Zongdi Feng, Stanley M. Lemon
Summary: In this Review, the authors discuss quasi-enveloped virions, their entry and release from human host cells, and their impact on host immunity and pathogenesis. They focus on hepatitis A and E viruses, which were previously considered non-enveloped but are now known to be released as quasi-enveloped virions cloaked in host membranes. Despite lacking virally encoded proteins on their surface, these virions efficiently enter cells and replicate. The authors also describe the mechanisms by which specific peptide sequences in the capsids of these virions mediate their release from hepatocytes and the current understanding of their cell entry mechanism.
NATURE REVIEWS MICROBIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Wenshi Wang, Florian A. Lempp, Franziska Schlund, Lisa Walter, Charlotte C. Decker, Zhenfeng Zhang, Yi Ni, Stephan Urban
Summary: Research has shown that HDVs from different genotypes replicate with varying efficacies. Variations in HDV genomes and HBV envelope proteins are major determinants of HDV egress and entry efficacy.
JOURNAL OF HEPATOLOGY
(2021)
Article
Medicine, General & Internal
Ming-Chao Tsai, Sien-Sing Yang, Chih-Che Lin, Wen-Lun Wang, Yao-Chun Hsu, Yaw-Sen Chen, Jui-Ting Hu, James Yu Lin, Ming-Lung Yu, Chih-Wen Lin
Summary: The study demonstrates a significant association between heavy alcohol intake and ALDH2 rs671 polymorphism with an increased risk of HCC development and mortality in patients with HBV-related cirrhosis.
Article
Chemistry, Medicinal
Xiao-Fei Chen, Xin Zhao, Zifeng Yang
Summary: Nucleic acid aptamers are single-stranded DNA or RNA molecules selected in vitro that exhibit high affinity and specificity in binding to a wide range of targets. They show promise as alternatives to conventional anti-infective agents and have potential in combating infectious diseases, including drug-resistant strains and biofilms. Aptamer-mediated drug delivery and controlled release strategies are also important in therapeutic approaches using aptamers.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Ruixue Wang, Saori Suzuki, Johnathan D. Guest, Brigitte Heller, Maricar Almeda, Alexander K. Andrianov, Alexander Marin, Roy A. Mariuzza, Zhen-Yong Keck, Steven K. H. Foung, Abdul S. Yunus, Brian G. Pierce, Eric A. Toth, Alexander Ploss, Thomas R. Fuerst
Summary: Hepatitis C virus (HCV) is a global disease burden that requires a preventive vaccine. The study found that a vaccine based on sE1E2.LZ can induce broadly neutralizing antibodies, indicating its potential as a promising vaccine candidate for further development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Zhaohui Li, Shihua Li, Gen Zhang, Weiyu Peng, Zhen Chang, Xue Zhang, Zheng Fan, Yan Chai, Feiran Wang, Xin Zhao, Dedong Li, Rong Zhang, Zhanlong He, Weiwei Zou, Ke Xu, Wenwen Lei, Peipei Liu, Junfeng Hao, Jingjing Zhang, Litao Sun, Guizhen Wu, Shuguang Tan, George Fu Gao, Feng Gao, Yan Wu
Summary: The development of a bispecific monoclonal antibody, bsAb15, based on B38 and H4 antibodies, shows improved neutralizing efficiency against SARS-CoV-2 and the ability to neutralize most variants of concern. Animal studies demonstrate the potential of bsAb15 for both prophylactic and therapeutic applications against severe COVID-19.
Article
Virology
Yingchong Wang, Qin Li, Dingwen Hu, Daolong Gao, Wenbiao Wang, Kailang Wu, Jianguo Wu
Summary: Research has identified the crucial role of ubiquitin-specific peptidase 38 (USP38) in host resistance against Zika virus infection by reducing viral polyubiquitination to inhibit infection. The deubiquitinase activity of USP38 is essential for inhibiting Zika virus infection.
Article
Gastroenterology & Hepatology
Garazi P. Alzua, Anne F. Pihl, Anna Offersgaard, Rodrigo Velazquez-Moctezuma, Carlos R. Duarte Hernandez, Elias H. Augestad, Ulrik Fahnoe, Christian K. Mathiesen, Henrik Krarup, Mansun Law, Jannick Prentoe, Jens Bukh, Judith M. Gottwein
Summary: This study identified key determinants of HCV infection and found that N-linked glycosylation motifs can trigger changes in the virus's sensitivity to neutralizing antibodies. These findings contribute to the understanding of how HCV evades antibodies and provide a theoretical basis for vaccine design.
Review
Gastroenterology & Hepatology
Alessandra Mangia, Rosa Cotugno, Giovanna Cocomazzi, Maria Maddalena Squillante, Valeria Piazzolla
Summary: The World Health Organization's goal of eliminating Hepatitis C virus (HCV) by 2030 using direct-acting antiviral treatments has become less attainable post the 2020 pandemic of coronavirus disease 2019. Therefore, a more realistic approach of micro-elimination in population segments where targeted strategies of prevention and treatment are easily attained has been promoted in Europe. Active involvement of stakeholders and community participation are essential components for the success of a micro-elimination project tailored at the geographical level according to HCV epidemiology and individual country's income.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Fisheries
Hongru Liang, Xiaozhe Fu, Qiang Lin, Lihui Liu, Zhibin Huang, Yinjie Niu, Luo Xia
Summary: In this study, modified aptamers were used to inhibit Grass carp reovirus (GCRV) infection, and PEGylation was applied to stabilize the aptamers, showing potential as therapeutic molecules against GCRV infection.
Review
Virology
Elsa Gomez-Escobar, Philippe Roingeard, Elodie Beaumont
Summary: The introduction of DAAs has transformed hepatitis C treatment by eliminating the virus without adverse effects. However, viral eradication remains challenging globally. The development of a vaccine containing HCV envelope glycoproteins, which are the main target for neutralizing antibodies, is crucial for reducing the burden of the disease. This review summarizes the targeted regions of these proteins and their presentation in current vaccine candidates.
Article
Virology
Yuwen Qin, Binbin Xue, Chunyan Liu, Xiaohong Wang, Renyun Tian, Qinya Xie, Mengmeng Guo, Guangdi Li, Darong Yang, Haizhen Zhu
JOURNAL OF VIROLOGY
(2017)
Article
Oncology
Chaohui Zuo, Xinyi Sheng, Zhuo Liu, Min Ma, Shuhan Xiong, Hongyu Deng, Sha Li, Darong Yang, Xiaohong Wang, Hua Xiao, Hu Quan, Man Xia
Article
Microbiology
Kattareeya Kumthip, Darong Yang, Nan L. Li, Yunzhi Zhang, Meiyun Fan, Aarti Sethuraman, Kui Li
Article
Oncology
Zhuo Liu, Min Ma, Lei Yan, Shilin Chen, Sha Li, Darong Yang, Xiaohong Wang, Hua Xiao, Hongyu Deng, Haizhen Zhu, Chaohui Zuo, Man Xia
Article
Gastroenterology & Hepatology
Chaohui Zuo, Yuan Hong, Xiaoxin Qiu, Darong Yang, Nianli Liu, Xinyi Sheng, Kunyan Zhou, Bo Tang, Shuhan Xiong, Min Ma, Zhuo Liu
Article
Engineering, Biomedical
Shuo Zhang, Xiaolin Sang, Dongxia Hou, Jinmei Chen, Hongwei Gu, Yujing Zhang, Jing Li, Darong Yang, Haizhen Zhu, Xiao Yang, Fangyu Wang, Chunni Zhang, Xi Chen, Ke Zen, Chen-Yu Zhang, Zhi Hong
Article
Infectious Diseases
Darong Yang, Nan L. Li, Dahai Wei, Baoming Liu, Fang Guo, Husni Elbahesh, Yunzhi Zhang, Zhi Zhou, Guo-Yun Chen, Kui Li
PLOS NEGLECTED TROPICAL DISEASES
(2019)
Article
Multidisciplinary Sciences
Yin Wu, Darong Yang, Runhua Liu, Lizhong Wang, Guo-Yun Chen
Article
Biochemistry & Molecular Biology
Yin Wu, Darong Yang, Guo-Yun Chen
Summary: Siglecs inhibit immune responses by recruiting tyrosine phosphatases through the ITIM domain, and their role in infection may be regulated by interaction with Gdi2 and Rab1a. Further research has revealed downstream signaling of Siglecs during bacterial infection.
CELLULAR AND MOLECULAR BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Yin Wu, Darong Yang, Guo-Yun Chen
Summary: This study demonstrates the essential role of Rab1a in embryonic development in mice, with homozygotes dying between E10.5 and E11.5. Rab1a is highly expressed in the small intestine, but its expression levels are low in the brains of embryos.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2022)
Article
Developmental Biology
Yin Wu, Darong Yang, Guo-Yun Chen
Summary: The study found that GDI2 and Rab1a regulate the immune response through interaction with the ITIM domain during bacterial infection. Gdi2 plays a crucial role in embryonic development, as complete loss of Gdi2 leads to embryonic lethality, while one functional Gdi2 allele is sufficient to maintain function.
Article
Multidisciplinary Sciences
Daron Yang, Yin Wu, Isaac Turan, Joseph Keil, Ku Li, Michael H. Chen, Runhua Liu, Lizhong Wang, Xue-Long Sun, Guo-Yun Chen
Summary: There is currently no effective therapy or antiviral against COVID-19 and SARS-CoV-2, and vaccines may be less effective against new variants. Therefore, it is urgent to understand the virulence mechanisms of SARS-CoV-2 and the host response in order to develop therapeutic agents.
Meeting Abstract
Immunology
Darong Yang, Yin Wu, Guo-Yun Chen
JOURNAL OF IMMUNOLOGY
(2020)
Article
Medicine, Research & Experimental
Xiaoxin Qiu, Shuhan Xiong, Yuan Hong, Xinyi Sheng, Bo Tang, Min Ma, Kunyan Zhou, Darong Yang, Lang Chen, Xiaohong Wang, Haizhen Zhu, Chaohui Zuo
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
(2019)
Meeting Abstract
Immunology
Darong Yang, Yin Wu, Guo-Yun Chen
JOURNAL OF IMMUNOLOGY
(2019)