Article
Biochemistry & Molecular Biology
Malgorzata Brauncajs, Filip Bielec, Anna Macieja, Dorota Pastuszak-Lewandoska
Summary: Gram-negative fermenting and non-fermenting bacteria, especially those that produce carbapenemases, are important etiological factors of nosocomial and community infections. New antibiotics and treatment regimens offer hope against these infections.
Article
Infectious Diseases
Chunquan Xu, Tao Chen, Siqin Zhang, Cui Zhou, Wenli Liao, Renchi Fang, Lijiang Chen, Tieli Zhou
Summary: This study investigated the in vitro activity of imipenem-relebactam alone and in combination with fosfomycin against carbapenem-resistant Gram-negative pathogens. The results showed that imipenem-relebactam in combination with fosfomycin had synergistic activity against Klebsiella pneumoniae and Acinetobacter baumannii. Imipenem-relebactam may be a new alternative for treating infections caused by carbapenem-resistant Gram-negative pathogens.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2022)
Article
Microbiology
Alina Dietrich, Ursula Steffens, Mike Gajdiss, Anna-Lena Boschert, Jana Katharina Droege, Christiane Szekat, Peter Sass, Imran T. Malik, Jan Bornikoel, Laura Reinke, Boris Macek, Mirita Franz-Wachtel, Kay Nieselt, Theresa Harbig, Kirstin Scherlach, Heike Broetz-Oesterhelt, Christian Hertweck, Hans-Georg Sahl, Gabriele Bierbaum
Summary: Resistance to antibiotics is a growing problem and alternative antimicrobial therapies are needed. Cervimycin, a polyketide antibiotic, shows promising activity against multidrug-resistant staphylococci and vancomycin-resistant enterococci. This study identified the Clp system and the essential kinase WalK as mutational hotspots for cervimycin resistance in Staphylococcus aureus. Furthermore, cervimycin-resistant strains exhibited vancomycin-intermediate resistance phenotype, suggesting a role of cervimycin in perturbing the bacterial cell envelope.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Desire Madi-Moussa, Yanath Belguesmia, Audrey Charlet, Djamel Drider, Francoise Coucheney
Summary: Antimicrobial resistance is a global health concern, and the use of antimicrobial peptides can effectively combat pathogenic bacteria. This study found that lacticaseicin 30, produced by Lacticaseibacillus paracasei, is active against Gram-negative clinical strains and can be used in combination with colistin to downregulate colistin resistance genes.
Article
Biochemistry & Molecular Biology
Azza Troudi, Jean Michel Bolla, Naouel Klibi, Jean Michel Brunel
Summary: Gram-negative bacteria pose a significant threat to public health due to the spread of drug resistance and the lack of effective alternative treatments. This study presents a promising strategy to combat resistant strains by combining a macrolide antibiotic with a polyaminoisoprenyl adjuvant derivative, resulting in a significant decrease in antibiotic resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Arsheena Yassin, Mariya Huralska, Jason M. Pogue, Deepali Dixit, Robert G. Sawyer, Keith S. Kaye
Summary: The prevalence of multidrug-resistant gram-negative bacterial infections has significantly increased in the past decade, leading to higher rates of morbidity and mortality. Treating these infections presents challenges, particularly for critically ill patients with limited margin for error. The availability of new therapies has improved treatment options, but optimal clinical and therapeutic approaches for managing these infections still need to be established.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Microbiology
Diletta Collalto, Alessandra Fortuna, Paolo Visca, Francesco Imperi, Giordano Rampioni, Livia Leoni
Summary: Co-administration of colistin and clofoctol enhances the bactericidal effect of colistin and reduces the minimum inhibitory concentration (MIC) of colistin in resistant strains. This study suggests the potential development of inhaled clofoctol-colistin formulations for the treatment of difficult-to-treat airway infections caused by drug-resistant Gram-negative pathogens.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Christine A. Pybus, Christina Felder-Scott, Victor Obuekwe, David E. Greenberg
Summary: The study compared the antimicrobial activity of Cefiderocol with seven commonly used antibiotics against multidrug-resistant pathogens, finding that Cefiderocol had potent inhibitory effects on biofilms of various pathogens, with consistently lower MIC90 values compared to other antibiotics.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Review
Pharmacology & Pharmacy
Dan Li, Fan Fei, Hua Yu, Xiangning Huang, Shanshan Long, Hao Zhou, Jie Zhang
Summary: This study systematically reviewed and compared the efficacy and posttreatment resistance of ceftazidime-avibactam therapy and ceftazidime-avibactam-based combination therapy in patients with Gram-negative pathogens. The results showed no significant difference in mortality rate, microbiologically negative, and clinical success between the two treatments, but a trend favoring the combination therapy in terms of posttreatment resistance was found. Additional high-quality studies are needed to further verify the conclusions.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Infectious Diseases
Muhammad Imran Sajid, Sandeep Lohan, Shun Kato, Rakesh Kumar Tiwari
Summary: This study demonstrates the significant antimicrobial activity of the physical mixture of cyclic [R4W4] peptide and levofloxacin against various drug-resistant bacterial strains, and it is more effective than the covalent conjugate. The cytotoxicity of the cyclic [R4W4] peptide and levofloxacin conjugates is lower in breast cancer cells and normal kidney cells. The time-kill kinetic study shows rapid bactericidal action of the combination of [R4W4] and levofloxacin against drug-resistant bacteria.
Article
Immunology
Qin Wang, Guiyi Liao, Quan Xia, Chaoliang Ge, Handong Ding
Summary: This study investigated the efficacy and adverse events of tigecycline therapy in renal transplant patients with carbapenem-resistant gram-negative bacterial infections. The results showed that tigecycline therapy appeared to be well tolerated in these patients, but attention should be paid to adverse reactions and close monitoring of laboratory tests.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Yuhang Wang, Junfeng Su, Ziyan Zhou, Jie Yang, Wenjuan Liu, Yafen Zhang, Pengyu Zhang, Tingting Guo, Guocai Li
Summary: As multidrug-resistant pathogens emerge and spread rapidly, novel antibiotics are urgently needed. Traditional Chinese medicine, such as baicalein, can be used as an adjuvant to enhance the efficacy of antibiotics. Baicalein was found to potentiate doxycycline against multidrug-resistant Gram-negative pathogens by disrupting the bacterial membrane and enhancing antibiotic entry. It can also increase the production of reactive oxygen species and inhibit multidrug efflux pumps and biofilm formation to potentiate antibiotic efficacy.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Dee Shortridge, Valerie Kantro, Mariana Castanheira
Summary: Carbapenems are commonly used for Gram-negative infections, but carbapenem-resistant Enterobacterales (CRE) is a major concern. Klebsiella pneumoniae serine carbapenemases (KPCs) and metallo-beta-lactamases (MBLs) are prevalent globally. Meropenem-vaborbactam, an inhibitor of serine carbapenemases, showed high susceptibility in U.S. MDR Enterobacterales isolates. KPC was the most common carbapenemase, while MBL and OXA-48-like carbapenemases were less common.
MICROBIOLOGY SPECTRUM
(2023)
Review
Infectious Diseases
Abdollah Ardebili, Ahdieh Izanloo, Mostafa Rastegar
Summary: The increasing prevalence of infections with multidrug-resistant (MDR), extensively-drug resistant (XDR) or difficult-to-treat (DTR) Gram-negative bacilli poses a severe challenge. Polymyxins, such as polymyxin B and colistin, have become a mainstay in clinical practices for treating these infections. However, the effect of polymyxin combination therapy versus monotherapy on treating Gram-negative bacilli-related infections is still unclear.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2023)
Article
Multidisciplinary Sciences
Tanshi Mehrotra, Dipasri Konar, Agila Kumari Pragasam, Shakti Kumar, Pradipta Jana, Prabhakar Babele, Deepjyoti Paul, Ayushi Purohit, Subhash Tanwar, Susmita Bakshi, Santanu Das, Jyoti Verma, Daizee Talukdar, Lekshmi Narendrakumar, Akanksha Kothidar, Sonali Porey Karmakar, Susmita Chaudhuri, Sujoy Pal, Kajal Jain, Chittur V. Srikanth, M. Jeeva Sankar, Krishnamohan Atmakuri, Ramesh Agarwal, Rajni Gaind, Mamatha Ballal, Nagamani Kammili, Rupak K. Bhadra, Thandavarayan Ramamurthy, G. Balakrish Nair, Bhabatosh Das
Summary: Microbes evolve rapidly through mutations and acquisition of mobile genetic elements (MGEs) associated with fitness traits like antimicrobial resistance (AMR). A multicentric study in India decoded genome sequences of bacterial pathogens causing sepsis, urinary tract infections, and respiratory infections. The study identified pathogen-specific AMR genes (ARGs) and MGEs, and differential abundance of resistance-associated proteins in the presence and absence of antibiotics, providing valuable insights for AMR diagnostics and antimicrobial therapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
