Article
Gastroenterology & Hepatology
Toer W. Stevens, Krisztina Gecse, Jerrold R. Turner, Gert de Hertogh, David T. Rubin, Geert R. D'Haens
Summary: A post hoc analysis of data from a phase 4 trial found that FC concentration can be used to discriminate patients with ongoing microscopic inflammation from patients with histologic remission even in patients with complete endoscopic healing of UC. The optimal cut-off concentration of FC is between 75 and 100 μg/g.
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Gastroenterology & Hepatology
Yu-Chen Pai, Li-Ting Weng, Shu-Chen Wei, Li-Ling Wu, David Q. Shih, Stephen R. Targan, Jerrold R. Turner, Linda Chia-Hui Yu
Summary: This study demonstrated the upregulation of TL1A, IFN gamma, and two MLCK variants in human IBD biopsy specimens. TL1A was found to induce MLCK-dependent changes in gut barrier function and bacterial internalization. Different MLCK isoforms were shown to orchestrate divergent pathways of bacterial infiltration in IBD, suggesting a potential mechanism for symptom flares in the disease.
JOURNAL OF CROHNS & COLITIS
(2021)
Article
Microbiology
Keiko Maeda, Nicholas C. Zachos, Megan H. Orzalli, Stefanie S. Schmieder, Denis Chang, Katlynn Bugda Gwilt, Michele Doucet, Nicholas W. Baetz, Sun Lee, Sue E. Crawford, Mary K. Estes, Jonathan C. Kagan, Jerrold R. Turner, Wayne Lencer
Summary: Polarized epithelial cells form a crucial barrier against infection, but certain viruses and bacterial toxins can enter the cells through the apical membrane. The loss of the cell polarity gene PARD6B and aPKC leads to the degradation of apical endosome function, rendering the cells resistant to further infection.
CELL HOST & MICROBE
(2022)
Article
Multidisciplinary Sciences
Wei-Ting Kuo, Matthew A. Odenwald, Jerrold R. Turner, Li Zuo
Summary: Epithelial cells are the first line of defense in mucosal defense. The tight junction, formed by interactions between claudins, zonula occludens, and tight junction-associated MARVEL protein (TAMP) families, plays a key role in sealing the paracellular space and preventing leakage. In addition to their canonical functions in barrier formation, ZO-1 and occludin also have essential noncanonical functions, including regulating apoptosis and proliferation, facilitating viral entry, and organizing specialized epithelial structures.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Michael J. Grey, Heidi De Luca, Beth A. McCormick, Jerrold R. Turner, Wayne I. Lencer
Meeting Abstract
Biochemistry & Molecular Biology
Jose Yeste, Xavi Illa, Nitesh Shashikanth, Anton Guimera-Brunet, Rosa Villa, Jerrold R. Turner
Meeting Abstract
Biochemistry & Molecular Biology
Sandra D. Chanez-Paredes, Weiqi He, Juanmin Zha, Li Zuo, W. V. Graham, Harry J. Rosenberg, Jerrold R. Turner
Meeting Abstract
Biochemistry & Molecular Biology
Shabnam Abtahi, Anne Sailer, Kamran Sadiq, Najeeha Iqbal, Kumail Ahmed, Furqan Kabir, S. A. Ali, Jerrold R. Turner
Article
Gastroenterology & Hepatology
Li Zuo, Wei-Ting Kuo, Feng Cao, Sandra D. Chanez-Paredes, Daniel Zeve, Prabhath Mannam, Lea Jean-Francois, Anne Day, W. Vallen Graham, Yan Y. Sweat, Nitesh Shashikanth, David T. Breault, Jerrold R. Turner
Summary: MLCK1 interacts with FKBP8 to be recruited to cell junctions, leading to immune-mediated barrier loss. This finding provides a critical foundation for therapeutic exploitation of FKBP8-MLCK1 interactions.
Article
Gastroenterology & Hepatology
Adam E. Mikolajczyk, Nathaniel A. Cohen, Sydeaka Watson, Max Ackerman, Sarah R. Goeppinger, John Hart, Jerrold R. Turner, David T. Rubin
Summary: The study found minimal variability in the degree of inflammation among biopsy fragments within and among different colorectal segments in UC patients, indicating that even a single biopsy could effectively represent the inflammation status of the entire colorectum. These findings have important implications for using histology as a clinical target and trial endpoint in UC.
INFLAMMATORY BOWEL DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Ekaterina Vasileva, Domenica Spadaro, Florian Rouaud, Jonathan M. King, Arielle Flinois, Jimit Shah, Sophie Sluysmans, Isabelle Mean, Lionel Jond, Jerrold R. Turner, Sandra Citi
Summary: ZO-1, the major scaffolding protein of tight junctions, recruits cingulin (CGN) and paracingulin (CGNL1) to tight junctions by binding to their N-terminal ZO-1 interaction motif. The binding of CGN to the C-terminal ZU5 domain of ZO-1 promotes ZO-1 stabilization and accumulation at tight junctions by promoting its extended conformation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Jan-Leo Kaak, Fabia D. Lobo de Sa, Jerrold R. Turner, Joerg-Dieter Schulzke, Roland Buecker
Summary: In this study, we investigated the mechanism by which microcystin leads to diarrhea. Our results showed that microcystin compromises the integrity of the intestinal epithelial barrier, resulting in increased permeability and diarrhea.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Immunology
Juan D. Matute, Jinzhi Duan, Magdalena B. Flak, Paul Griebel, Jose A. Tascon-Arcila, Shauni Doms, Thomas Hanley, Agne Antanaviciute, Jennifer Gundrum, Jessica L. Mark Welch, Brandon Sit, Shabnam Abtahi, Gwenny M. Fuhler, Joep Grootjans, Florian Tran, Stephanie T. Stengel, James R. White, Niklas Krupka, Dirk Haller, Simon Clare, Trevor D. Lawley, Arthur Kaser, Alison Simmons, Jonathan N. Glickman, Lynn Bry, Philip Rosenstiel, Gary Borisy, Matthew K. Waldor, John F. Baines, Jerrold R. Turner, Richard S. Blumberg
Summary: ITLN1, regulated by the unfolded protein response, coats certain microorganisms to thin the inner mucus layer and aggravates colitis. Its upregulation in UC patients is related to dysregulation of the unfolded protein response, affecting the composition of the inner mucus layer and increasing sensitivity to colitis.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Review
Gastroenterology & Hepatology
Clara Abraham, Maria T. Abreu, Jerrold R. Turner
Summary: This review focuses on the role of pattern recognition receptors in the inflammatory response and regulation of epithelial barrier in inflammatory bowel disease. It explores the cytokine networks that contribute to inflammation, regulation of paracellular permeability, epithelial damage, up-regulation of epithelial proliferation, and restitutive processes. Patient samples are used in studies and potential pathways for a more comprehensive treatment approach for patients with inflammatory bowel disease are speculated.
Article
Medicine, Research & Experimental
Michael J. Grey, Heidi De Luca, Doyle V. Ward, Irini A. M. Kreulen, Katlynn Bugda Gwilt, Sage E. Foley, Jay R. Thiagarajah, Beth A. McCormick, Jerrold R. Turner, Wayne I. Lencer
Summary: Epithelial cells lining mucosal surfaces of the gastrointestinal and respiratory tracts express ERN2/IRE1 beta, which prevents ER stress in goblet cells by splicing Xbp1 mRNA and regulates the assembly of the colon mucus layer and gut microbiota composition.
JOURNAL OF CLINICAL INVESTIGATION
(2022)