Article
Cell Biology
Beth C. Bragdon, Andrew Bennie, Amanda Molinelli, Yu Liu, Louis C. Gerstenfeld
Summary: There is currently no consensus on whether there is a single or multiple population(s) of postnatal stem cells that contribute to skeletal homeostasis and postnatal bone formation. This study found that a population of cells expressing Prx1 contributes to postnatal bone formation. Using a transgenic mouse model, the researchers tracked the lineage of Prx1 expressing cells and found recruitment and expansion of these cells in newly formed bone tissues during fracture and ectopic bone formation. Prx1-derived cells were limited or absent at axial skeletal sites, and could differentiate into multiple cell lineages.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Michele C. Larsen, Catherine M. Rondelli, Ahmed Almeldin, Yong-Seok Song, Alhaji N'Jai, David L. Alexander, E. Camilla Forsberg, Nader Sheibani, Colin R. Jefcoate
Summary: This study reveals the regulation of bone marrow cells by polycyclic aromatic hydrocarbon (PAH) pollutants and microbiome products through the aryl hydrocarbon receptor (AhR). The absence of Cyp1b1 and activation of AhR by PAH result in similar gene stimulations in these cells. Specific sources of Cyp1b1 are found to be linked to AhR and an OLFR network to modulate BM inflammation through diverse microbiome products.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Camila Medrano-Trochez, Paramita Chatterjee, Pallab Pradhan, Hazel Y. Stevens, Molly E. Ogle, Edward A. Botchwey, Joanne Kurtzberg, Carolyn Yeago, Greg Gibson, Krishnendu Roy
Summary: This study showed that donor differences in hMSC transcriptome are minor compared to the intrinsic differences in tissue-of-origin. hMSCs with different transcriptomic profiles exhibited potential differences in functional characteristics, contributing to the understanding of differences in out-of-thaw therapeutic hMSC products.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Pharmacology & Pharmacy
Masaaki Ishibashi, Masakazu Ishii, Shinichiro Yamamoto, Yasuo Mori, Shunichi Shimizu
Summary: The study demonstrates that 5-FU enhances the activation of TRPM2 channel, and inhibition of H2O2-induced Ca2+ concentration increase by 5-FU in Trpm2 KO mice, alleviating the reduction of blood cells induced by 5-FU.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Cell Biology
Mario Barilani, Christopher Lovejoy, Roberta Piras, Andrey Y. Abramov, Lorenza Lazzari, Plamena R. Angelova
Summary: The study found that aging in MSCs is associated with changes in mitochondrial metabolism, including a decrease in mitochondrial membrane potential, lower NADH levels, and higher mitochondrial DNA content. Despite higher ATP levels in aged cells, increased rates of mitophagy lead to decreased overall mitochondrial mass. Additionally, changes in mitochondrial metabolism in aged MSCs result in the overproduction of reactive oxygen species in mitochondria, which is compensated by higher levels of the endogenous antioxidant glutathione.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Orthopedics
Ava Brozovich, Brent J. Sinicrope, Guillermo Bauza, Federica Banche Niclot, David Lintner, Francesca Taraballi, Patrick C. McCulloch
Summary: This study aimed to characterize bone marrow aspirate (BMA) versus bone marrow aspirate concentrate (BMAC) for mesenchymal stem cell (MSC) quantity, potency, and cytokine profile. The results showed significantly higher MSC concentration and CFU number in BMA compared to BMAC, with BMAC failing to concentrate MSC in 6 out of 8 samples.
ORTHOPAEDIC JOURNAL OF SPORTS MEDICINE
(2021)
Review
Cell & Tissue Engineering
Zahra Azizi, Roya Abbaszadeh, Roxana Sahednasagh, Amir Norouzy, Elahe Motevaseli, Kathrin Maedler
Summary: Bone marrow mesenchymal stromal cells have anti-inflammatory and pro-survival properties, and their fusion with transplanted islets can improve diabetes treatment.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Verena Petzer, Igor Theurl, Gunter Weiss, Dominik Wolf
Summary: Systemic iron overload in patients with MDS is multifactorial, mainly due to ineffective erythropoiesis and chronic red blood cell transfusion. Iron homeostasis varies among different MDS subtypes and its role in MDS is increasingly recognized. Genetic alterations, such as mutations in TET2, can lead to disturbances in iron homeostasis and hematopoiesis, highlighting the need for comprehensive understanding and development of future therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kai Zhao, Dong Han, Si-Rui He, Long-Yan Wu, Wu-Yang Liu, Zhao-Ming Zhong
Summary: Bone marrow endothelial cells (BMECs) are crucial for maintaining bone homeostasis. The decline in BMECs is associated with abnormal bone development and loss. The mechanism of age-related oxidative stress enhancement in BMEC dysfunction remains unclear. This study investigated the injury caused by oxidative stress enhancement in BMECs and evaluated the potential therapeutic effect of N-Acetyl-L-cysteine (NAC) in senile osteoporosis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Jessie Atterholt, Holly N. Woodward
Summary: The study examined the bone microanatomy of crown-group birds from different major clades, analyzing the variations in humerus and femur tissues at different growth stages and their correlation with developmental modes. The research found that bone tissue characteristics in birds are closely related to developmental patterns and body size differences, indicating the importance of timing in altering growth trajectories and adult body size.
Review
Medicine, Research & Experimental
Ye Chen, Feng Zhou, Hanghang Liu, Jiaxuan Li, Huiling Che, Jiaqi Shen, En Luo
Summary: SIRT1, a NAD+-dependent deacetylase, plays a crucial role in regulating bone homeostasis by controlling the balance between osteoblasts and osteoclasts, enhancing bone-forming cells' viability, and maintaining bone vasculature homeostasis. This suggests that SIRT1 could be a potential target for manipulation and therapy in bone-related disorders.
Article
Biochemistry & Molecular Biology
Elia Bari, Ilaria Roato, Giuseppe Perale, Filippo Rossi, Tullio Genova, Federico Mussano, Riccardo Ferracini, Marzio Sorlini, Maria Luisa Torre, Sara Perteghella
Summary: A new strategy was developed to improve the osteoinductivity of SmartBone (SB) by loading the SB scaffolds with freeze-dried formulation of mesenchymal stem cell (MSC)-secretome. The study showed that SBlyo significantly enhanced cell proliferation and tissue formation compared to SB, suggesting a more effective bone formation induction by SBlyo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Geriatrics & Gerontology
Maria Luz Torres, Nahuel Ezequiel Wanionok, Antonio Desmond McCarthy, Gustavo Ramon Morel, Juan Manuel Fernandez
Summary: The study revealed for the first time in an aged rat model a relationship between alterations in bone quality and memory impairment, with increased systemic oxidative stress as a possible unifying mechanism.
EXPERIMENTAL GERONTOLOGY
(2021)
Article
Oncology
Francesca Liccardo, Martyna Sniegocka, Claudia Tito, Alessia Iaiza, Tiziana Ottone, Mariadomenica Divona, Serena Travaglini, Maurizio Mattei, Rosella Cicconi, Selenia Miglietta, Giuseppe Familiari, Stefania Annarita Nottola, Vincenzo Petrozza, Luca Tamagnone, Maria Teresa Voso, Silvia Masciarelli, Francesco Fazi
Summary: In this study, a combination therapy of low doses of retinoic acid (R), bortezomib (B), and arsenic trioxide (A) was found to induce proteotoxic stress and oxidative stress in FLT3-ITD+ AML cells, resulting in strong cytotoxic activity. The protective role of bone marrow stromal cells (BMSCs) in AML cells was also demonstrated, but this protection can be overcome by using high doses of ascorbic acid (Vitamin C) as an adjuvant. Importantly, the combination of RBA and ascorbic acid significantly prolonged the lifespan of a murine model of FLT3-ITD+ AML without toxic effects.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Geriatrics & Gerontology
Pan Fan, Xiao-Yu Yu, Chang-Hong Chen, Jia-Wei Gao, Yu-Zhu Xu, Xin-Hui Xie, Yun-Tao Wang
Summary: The study found that TNF-alpha caused transient senescent damage to BMSCs in the early stage, but this damage was alleviated through Parkin-mediated mitophagy which maintained mitochondrial homeostasis. Inhibiting mitophagy irreversibly led to cellular senescence induced by TNF-alpha. These results suggest that Parkin-mediated mitophagy plays a protective role in BMSCs in response to TNF-alpha and could be an important therapeutic target in the future.
EXPERIMENTAL GERONTOLOGY
(2022)
Article
Cell & Tissue Engineering
Methichit Wattanapanitch, Alisa Ritthaphai, Chanmi Park, Bootsakorn Boonkaew, Ratchapong Netsrithong, Kovit Pattanapanyasat, Pattarawan Limsiri, Chinnavuth Vatanashevanopakorn
STEM CELL RESEARCH
(2018)
Article
Cell & Tissue Engineering
Ponthip Potirat, Methichit Wattanapanitch, Vip Viprakasit, Pakpoom Kheolamai, Surapol Issaragrisil
STEM CELL RESEARCH
(2019)
Article
Cell & Tissue Engineering
Sermporn Thaweesapphithak, Chairat Tantrawatpan, Pakpoom Kheolamai, Duangrat Tantikanlayaporn, Sittiruk Roytrakul, Sirikul Manochantr
STEM CELL RESEARCH & THERAPY
(2019)
Review
Cell & Tissue Engineering
Methichit Wattanapanitch
STEM CELLS INTERNATIONAL
(2019)
Article
Cell & Tissue Engineering
Nutnicha Tantarungsee, Zaukir Bostan Ali, Rungtip Soi-ampornkul, Chuda Rujitharanawong, Chanika Subchookul, Alisa Ritthaphai, Bootsakorn Boonkaew, Methichit Wattanapanitch, Kanchalit Thanomkitti, Daranporn Triwongwaranat, Chinnavuth Vatanashevanopakorn, Rattapon Thuangtong
STEM CELL RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
V. Trakoolwannachai, P. Kheolamai, S. Ummartyotin
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Editorial Material
Cell & Tissue Engineering
Ratchapong Netsrithong, Nutchanawan Promnakhon, Bootsakorn Boonkaew, Chinnavuth Vatanashevanopakorn, Kovit Pattanapanyasat, Methichit Wattanapanitch
STEM CELL RESEARCH
(2019)
Article
Multidisciplinary Sciences
Pattama Ekpo, Naharuthai Inthasin, Sutthicha Matamnan, Patimaporn Wongprompitak, Methichit Wattanapanitch, Chawikan Boonwong, Chareenun Chirapapaisan, Panotsom Ngowyutagon, Mongkol Uiprasertkul, Pinnita Prabhasawat, Wiwit Tantibhedhyangkul
Review
Cell Biology
Siwanon Jirawatnotai, Stephen Dalton, Methichit Wattanapanitch
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Kongtana Trakarnsanga, Chartsiam Tipgomut, Chanatip Metheetrairut, Methichit Wattanapanitch, Archrob Khuhapinant, Saiphon Poldee, Ryo Kurita, Yukio Nakamura, Chatchawan Srisawat, Jan Frayne
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Bootsakorn Boonkaew, Siriwal Suwanpitak, Kovit Pattanapanyasat, Nuttawut Sermsathanasawadi, Methichit Wattanapanitch
Summary: This study successfully generated induced pluripotent stem cells (iPSCs) from a patient with PAD and successfully differentiated them into functional endothelial cells. This approach may provide a renewable source of large-scale endothelial cells for the treatment of PAD.
CELL AND TISSUE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Koollawat Chupradit, Wannisa Khamaikawin, Supachai Sakkhachornphop, Chaniporn Puaninta, Bruce E. Torbett, Suparerk Borwornpinyo, Suradej Hongeng, Methichit Wattanapanitch, Chatchai Tayapiwatana
Summary: The study demonstrates the efficacy and safety of using 2LTRZFP in human CD34(+) HSPCs for gene therapy in HIV-1-infected individuals. The 2LTRZFPmCherry-transduced macrophages showed normal function and phenotype, and exhibited resistance to HIV-1 integration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Nontaphat Thongsin, Methichit Wattanapanitch
Summary: Allogeneic cell therapy shows promise in regenerative medicine, but rejection due to mismatched HLAs is a concern. A homozygous B2M knockout iPSC line lacking HLA-I expression was generated using CRISPR/Cas9. This iPSC line serves as an in vitro model for studying immune responses to allogeneic grafts and offers a prototype for off-the-shelf allogeneic cell products in cell-based therapy.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Ratchapong Netsrithong, Siriwal Suwanpitak, Bootsakorn Boonkaew, Kongtana Trakarnsanga, Lung-Ji Chang, Chartsiam Tipgomut, Chinnavuth Vatanashevanopakorn, Kovit Pattanapanyasat, Methichit Wattanapanitch
STEM CELL RESEARCH & THERAPY
(2020)
