Article
Biochemistry & Molecular Biology
Hajar A. Asal, Kamel R. Shoueir, Maha A. El-Hagrasy, Elshahat A. Toson
Summary: Chitosan-based nanoparticles were developed as nanocarriers for insulin to enhance its oral uptake. Among them, chitosan gold nanoparticles functionalized with poly lactic-co-glycolic acid (PLGA) showed high insulin retention in an acidic medium, resulting in reduced blood glucose levels and improved pharmacological availability and bioavailability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Pharmacology & Pharmacy
Carlynne Choy, Lee Yong Lim, Lai Wah Chan, Zhixiang Cui, Shirui Mao, Tin Wui Wong
Summary: This review examines the potential of targeting intestinal receptors to improve the oral delivery of nanoinsulin. While promising, further research and optimization are needed.
PHARMACOLOGICAL REVIEWS
(2022)
Article
Engineering, Biomedical
Weizhi Wang, Chenggong Yu, Fangfang Zhang, Yuxuan Li, Bo Zhang, Jie Huang, Zhijun Zhang, Liang Jin
Summary: A drug delivery system, GC-CA@PLGA NPs, has been developed to improve the oral administration of insulin with excellent stability, good biocompatibility, and significantly increased bioavailability for potential application in the treatment of diabetes.
BIOMEDICAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Yun Liu, Yanfang Wang, Yuejun Yao, Juan Zhang, Wei Liu, Kangfan Ji, Xinwei Wei, Yuanwu Wang, Xiangsheng Liu, Shiming Zhang, Jinqiang Wang, Zhen Gu
Summary: Lipid nanoparticle-based drug delivery systems have a significant impact on nucleic acid-based therapy and vaccination. We designed phenylboronic acid-based quaternary amine-type cationic lipids that can self-assemble into spherical lipid nanoparticles. Mixing insulin with the lipid nanoparticles immediately forms a heterostructured insulin complex, and in a hyperglycemia-relevant glucose solution, the lipid nanoparticles gradually become less positively charged, leading to insulin release.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Pharmacology & Pharmacy
Amani Elsayed, Mayyas Al-Remawi, Nisrein Jaber, Khalid M. Abu-Salah
Summary: Diabetes mellitus is a metabolic endocrine disease characterized by chronic hyperglycemia. Insulin therapy is crucial for its management, with subcutaneous administration being the most common route. To improve oral insulin absorption, novel approaches in the field of nanoscience and nanotechnology have been explored, including the use of nanoparticles, nano-platforms, and nanocarriers.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Review
Oncology
Mohammad Mirza-Aghazadeh-Attari, Ainaz Mihanfar, Bahman Yousefi, Maryam Majidinia
Summary: Melatonin, also known as N-[2-(5-methoxy-1H-indol-3-yl) ethyl], is a biogenic amine with a wide range of biological functions and therapeutic potential. However, its application may lead to unwanted effects. The use of nanoparticles as delivery systems for melatonin can prevent its degradation in circulation and improve its pharmacokinetic profile.
CANCER CELL INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Kedong Liu, Yun Chen, Zhaoqi Yang, Jian Jin
Summary: In recent years, zwitterionic materials have gained attention in oral drug delivery system for their ability to diffuse rapidly through mucus and enhance cellular internalization. However, directly coating hydrophobic nanoparticles with zwitterionic materials has been a challenge due to their strong polarity. This study developed a simple and convenient strategy using zwitterionic Pluronic analogs to coat nanoparticles. The results showed that the zwitterionic Pluronic analogs-coated nanoparticles were stable, effectively overcame mucus and epithelium barriers, and utilized transport pathways for enhanced internalization and cellular transport. These nanoparticles also exhibited improved absorption and distribution compared to other nanoparticles, and showed promising potential for oral delivery of biotherapeutics, as demonstrated by the fine hypoglycemic response in diabetic rats after oral administration of insulin-loaded nanoparticles.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Mahboubeh Ebrahimian, Fatemeh Mahvelati, Bizhan Malaekeh-Nikouei, Ezzat Hashemi, Fatemeh Oroojalian, Maryam Hashemi
Summary: A lipopolymeric nanoparticle containing bromelain was developed to enhance its stability and oral delivery efficiency. The results showed that this nanoparticle had higher encapsulation efficiency and released more bromelain in different pH environments. It also demonstrated higher absorption through Caco-2 cells compared to other nanoparticles.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Aun Raza, Jared A. Miles, Fekade Bruck Sime, Benjamin P. Ross, Jason A. Roberts, Amirali Popat, Tushar Kumeria, James R. Falconer
Summary: A novel multi-material delivery system based on gamma-CD and PLGA was developed to enhance the solubility and stability of MER, while maintaining its antibacterial activity.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Jonas Schreiner, Christopher Rindt, Jana Wachter, Nathalie Jung, Sarah Vogel-Kindgen, Maike Windbergs
Summary: The physicochemical properties of nanocarriers are crucial for oral drug delivery. The composition of the nanocarriers and the assembly process affect these properties. The molecular weight of the drug has an impact on the physicochemical properties of nanocarriers and their permeation through the intestinal epithelium. Intestinal absorption depends on both tissue properties and carrier system properties. The molecular weight of the drug payload is a key factor in determining the properties of polymeric nanocarriers and their oral absorption.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Engineering, Biomedical
Hongbo Cheng, Zhixiang Cui, Shuang Guo, Xin Zhang, Yingnan Huo, Shirui Mao
Summary: Mucoadhesive and mucopenetrating nanoparticles have different absorption mechanisms in enhancing oral insulin absorption. Mucopenetrating nanoparticles have a faster onset of action, while mucoadhesive nanoparticles show a sustained hypoglycemic effect. Overall, there is no significant difference in pharmacological availability between the two types of nanoparticles.
ACTA BIOMATERIALIA
(2021)
Article
Chemistry, Multidisciplinary
Mengjun Zhang, Huiyang Jiang, Lan Wu, Haoyu Lu, Hriday Bera, Xing Zhao, Xiong Guo, Xulu Liu, Dongmei Cun, Mingshi Yang
Summary: This study demonstrates the successful delivery of siRNA to AECs using inhaled lipid nanoparticles (LNPs) targeting ICAM-1 receptors, resulting in reduced expression of proinflammatory cytokines in AECs and effective alleviation of symptoms in asthmatic mice.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Materials Science, Biomaterials
De-feng Li, Mei-feng Yang, Hao-ming Xu, Min-zheng Zhu, Yuan Zhang, Cheng-mei Tian, Yu-qiang Nie, Jian-yao Wang, Yu-jie Liang, Jun Yao, Li-sheng Wang
Summary: This article summarizes the pathophysiological features of inflammatory bowel disease (IBD) and discusses the strategies and recent developments in using nanoparticle technology for targeted therapy in the intestines.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Pharmacology & Pharmacy
Maria B. Sokol, Margarita Chirkina, Nikita G. Yabbarov, Mariia R. Mollaeva, Tatyana A. Podrugina, Anna S. Pavlova, Viktor V. Temnov, Rania M. Hathout, Abdelkader A. Metwally, Elena D. Nikolskaya
Summary: In this study, a combination of molecular dynamics simulation and in vitro experiments was used to improve the design of polymer-based nanoparticles for cancer treatment. The results showed that nanoparticles loaded with novel carboplatin derivatives exhibited high antitumor activity and hemocompatibility, indicating the potential for optimizing encapsulation and antitumor efficacy in drug delivery system design.
Article
Chemistry, Multidisciplinary
Xin Li, Holmfridur R. Halldorsdottir, Sven Weller, Anna Colliander, Martin Bak, Paul Kempen, Gael Clergeaud, Thomas L. Andresen
Summary: Although adoptive T cell therapy for cancer treatment has limitations, loading T cells with SHP099 can enhance therapeutic efficacy. Cell-loaded SHP099 enables sustained inhibition of the PD-1/PD-L1 signaling and increases cytolytic activity of the T cells. This is a promising therapeutic strategy.
Article
Chemistry, Physical
Xin Zhou, Anders Wilgaard Sinkjaer, Min Zhang, Henrik Dahl Pinholt, Hanne Morck Nielsen, Nikos S. Hatzakis, Marco van de Weert, Vito Fodera
Summary: Amyloid aggregation is associated with diseases and therapeutic protein formulations. Co-solutes can modulate protein stability and work as inhibitors for drug design. This study uses insulin and polysorbate 80 to analyze the heterogeneous multicomponent system at a single aggregate level and reveals the inhibition mechanism depends on growth pathways. The method is a general tool for physicochemical characterization of self-assembly reactions.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Medicine, Research & Experimental
Ragna G. Diedrichsen, Pernille S. Tuelung, Vito Fodera, Hanne M. Nielsen
Summary: The stereochemistry of carrier peptides affects their interaction with insulin and insulin delivery. The permeation of insulin through the intestinal epithelium is influenced by the degree of insulin-carrier peptide complexation and depends on the stereochemistry of penetramax. The L-form peptides initially decrease epithelial integrity, but this effect is reversible.
MOLECULAR PHARMACEUTICS
(2023)
Article
Nanoscience & Nanotechnology
Matteo Tollemeto, Zheng Huang, Jorn B. Christensen, Hanne Morck Nielsen, Stine Ronholt
Summary: Biological drugs are increasingly important for the treatment of diseases such as diabetes, cancer, and obesity. This project explores the potential of using mucoadhesive delivery systems based on dendron-coated mesoporous silica nanoparticles for oral delivery of biological drugs. The study focuses on the interaction between dendrons and mucin, and successfully conjugates dendrons onto mesoporous silica nanoparticles. The interaction between the nanoparticles and mucin or intestinal mucus is investigated, providing knowledge for the development of mucoadhesive nanoformulations for oral delivery of biopharmaceuticals.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Shunliang Wu, Mads Ostergaard, Freja Fredholt, Niels Johan Christensen, Kasper K. Sorensen, Narendra K. Mishra, Hanne M. Nielsen, Knud J. Jensen
Summary: Chemical modification of peptides and proteins, such as PEGylation and lipidation, can create new conjugates with different properties. However, these conjugates are often not responsive to stimuli. This study reports the Ca2+-responsive properties of conjugates of oligogalacturonic acids (OGAs), derived from plant pectin. The self-assembly of OGA conjugates was studied and found to depend on OGA sequence length, as well as Zn2+ and Ca2+ concentrations.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Chemistry, Applied
Mai Bay Stie, Heidi Oblom, Anders Christian Norgaard Hansen, Jette Jacobsen, Ioannis S. Chronakis, Jukka Rantanen, Hanne Morck Nielsen, Natalja Genina
Summary: The study designed a multi-layered nanofiber-on-foam-on-film (NFF) drug delivery system with mucoadhesive properties and saliva-repelling ability, which improved drug absorption and permeability through oral mucosa. This system has great potential for non-invasive drug administration.
CARBOHYDRATE POLYMERS
(2023)
Article
Pharmacology & Pharmacy
Ravi Maharjan, Shavron Hada, Ji Eun Lee, Hyo-Kyung Han, Ki Hyun Kim, Hye Jin Seo, Camilla Foged, Seong Hoon Jeong
Summary: A combinatorial artificial-neural-network design-of-experiment (ANN-DOE) model was developed to evaluate the effects of various factors on the outcome responses of mRNA-LNP vaccine. The ANN-DOE model outperformed optimized ML models in predicting the bioprocess of the vaccine. Increased flow rate ratio (FRR) decreased particle size and increased zeta potential (ZP), while increased total flow rate (TFR) increased PDI and ZP. The ANN-DOE model demonstrated better predictive ability and accuracy compared to independent ML models.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Correction
Chemistry, Physical
Xin Zhou, Dirk Fennema Galparsoro, Anders Ostergaard Madsen, Valeria Vetri, Marco van de Weert, Hanne Morck Nielsen, Vito Fodera
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Physical
You Xu, Elisa Parra-Ortiz, Feng Wan, Olga Canadas, Begona Garcia-Alvarez, Aneesh Thakur, Henrik Franzyk, Jesus Perez-Gil, Martin Malmsten, Camilla Foged
Summary: Pulmonary delivery of siRNA using nanoparticle-based systems shows promise for treating respiratory diseases. We developed inhaler formulations of siRNA-loaded lipid-polymer hybrid nanoparticles and optimized them for inhalation therapy. The interaction between the nanoparticles and pulmonary surfactant was studied to understand the mechanisms involved. The results provide new insights into the design criteria for nanoparticle transport through the air-blood barrier.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Marco Herrera-Barrera, Milan Gautam, Abhijeet Lokras, Kseniia Vlasova, Camilla Foged, Gaurav Sahay
Summary: Prime editing is an advanced gene editing platform that can correct almost any disease-causing mutation. This study developed lipid nanoparticles as delivery vehicles for prime editors, which resulted in improved efficiency and speed of editing. The use of these nanoparticles has the potential to revolutionize gene therapy and open up new possibilities for treatment.
Article
Chemistry, Multidisciplinary
Jessica Pihl, Thomas M. M. Clausen, Jiarong Zhou, Nishta Krishnan, Maj S. S. Orum-Madsen, Tobias Gustavsson, Robert Dagil, Mads Daugaard, Swati Choudhary, Camilla Foged, Jeffrey D. D. Esko, Liangfang Zhang, Ronnie H. H. Fang, Ali Salanti
Summary: Malaria-infected erythrocytes and oncofetal CS have distinct tropism and can potentially be used for cancer targeting. Researchers have developed a drug delivery platform using erythrocyte membrane-coated nanoparticles that mimic malaria-infected erythrocytes and specifically target and kill melanoma cells. This biomimetic approach shows potential as a broadly targeted cancer therapy.
Article
Pharmacology & Pharmacy
Aneesh Thakur, Abishek Wadhwa, Abhijeet Lokras, Olivia Amanda Oest Mullertz, Dennis Christensen, Henrik Franzyk, Camilla Foged
Summary: The incorporation of ionizable lipid L5N12 in liposomal cationic adjuvant CAF (R) 09 maintains the adjuvant function and induces antigen-specific immune responses comparable to unmodified CAF (R) 09.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Marie Hellfritzsch, Dennis Christensen, Camilla Foged, Regina Scherliess, Aneesh Thakur
Summary: Reconstituted dry powder formulations of ZnO nanoparticles can induce antigen-specific antibodies and can be used in vaccines to enhance antigen-specific humoral immune responses against subunit protein antigens.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Physical
Ragna Guldsmed Diedrichsen, Valeria Vetri, Sylvain Prevost, Vito Fodera, Hanne Morck Nielsen
Summary: The cell-penetrating peptide penetratin and its analogues shuffle and penetramax have been investigated for their potential as carrier peptides for oral drug delivery. Through advanced biophysical techniques, it was found that these peptides induced liposome clustering, with distinctively different types of membrane interactions observed. Furthermore, these interactions did not disrupt the liposomes, making them advantageous for drug delivery applications. This detailed insight into peptide-membrane interactions is crucial for understanding the mechanism of peptide-based excipients and the influence of peptide sequence modifications.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Nanoscience & Nanotechnology
Miriam E. van Gent, Sylvia N. Kiodsinska, Maureen Severin, Muhanad Ali, Bjorn R. van Doodewaerd, Erik Bos, Roman I. Koning, Jan Wouter Drijfhout, Hanne M. Nielsen, Peter H. Nibbering
Summary: Hyaluronic acid-based nanogels were used to encapsulate the antimicrobial peptide Ab-Cath, overcoming its limitations and reducing its cytotoxic effects. The nanogels effectively killed antibiotic-resistant bacteria and eliminated bacteria on human skin. This study highlights the potential of nanogels to enhance the selectivity of antimicrobial peptides.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Miriam E. van Gent, Sylvia N. Klodzinska, Jan Wouter Drijfhout, Hanne M. Nielsen, Peter H. Nibbering
Summary: This study evaluated the encapsulation of two antimicrobial peptides (AMPs) into nanogels to improve their selectivity index. The nanogels showed similar activity to AMP solutions against antimicrobial resistant bacteria and reduced cytotoxicity towards human cells, thereby increasing their potential as therapeutics.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)