Article
Chemistry, Applied
Di Lan, Jiali Zhang, Xiaolei Shang, Lijuan Yu, Chen Xu, Pin Wang, Lige Cui, Nanqi Cheng, Huifang Sun, Jianing Ran, Le Sha, Ronghua Yin, Na Gao, Jinhua Zhao
Summary: This study extracted fucosylated chondroitin sulfate (FCS) from Phyllophorella kohkutiensis and analyzed its structure. The researchers found that PkFCS has diverse branch structures and potential biological activities in terms of anticoagulant and anti-iXase properties.
CARBOHYDRATE POLYMERS
(2023)
Article
Chemistry, Applied
Shoichi Onishi, Kento Shionoya, Kazuki Sato, Ayumu Mubuchi, Shiori Maruyama, Tadaaki Nakajima, Masahiro Komeno, Shinji Miyata, Kazumi Yoshizawa, Takeshi Wada, Robert J. Linhardt, Toshihiko Toida, Kyohei Higashi
Summary: The study investigates the glycosaminoglycans (GAGs) derived from marine organisms and finds that marine invertebrates, especially Bivalvia, are rich sources of highly sulfated or branched GAGs. A novel fucosylated heparan sulfate (Fuc-HS) is identified from the midgut gland of the Japanese scallop, Patinopecten yessoensis. Scallop HS exhibits resistance to GAG-degrading enzymes and shows anti-coagulant and neurite outgrowth-promoting activities. These findings suggest that the midgut gland of scallops is a valuable source of biologically active Fuc-HS.
CARBOHYDRATE POLYMERS
(2023)
Article
Materials Science, Multidisciplinary
Rui R. Costa, David Caballero, Diana Soares da Costa, Romen Rodriguez-Trujillo, Subhas C. Kundu, Rui L. Reis, Iva Pashkuleva
Summary: Anisotropy plays a crucial role in the organization and healing of neural tissues, and holds great potential for developing regeneration strategies. The study demonstrates the fabrication of microfibers from charged ECM components using interfacial polyelectrolyte complexation, which can mimic the mechanical properties of neural tissues. These fibers are biocompatible and promote the formation of neural processes, enabling the development of neural cells.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Biology
Lynn Kruger, Karina Biskup, Vasileios Karampelas, Antje Ludwig, Antje-Susanne Kasper, Wolfram C. Poller, Veronique Blanchard
Summary: In this study, an optimized strategy was presented to analyze glycosaminoglycan disaccharides via MALDI-TOF mass spectrometry, successfully identifying sulfation positions and patterns through fragmentation experiments.
Article
Microbiology
Annelieke Overbeeke, Bela Hausmann, Georgi Nikolov, Fatima C. Pereira, Craig W. Herbold, David Berry
Summary: The study found that not all gut bacteria capable of degrading chondroitin sulfate could also degrade hyaluronan, despite having lyases that act on both compounds. In the model organism Bacteroides, a lyase along with surface binding proteins may be involved in hyaluronan breakdown, and degradation of these compounds provides public goods for other bacteria. Cooperative degradation and cross-feeding may be widespread in mucosal glycan utilization.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fa-kun Dong, Xian-gao Quan, Qing-bing Wang, Zhao-ming Liu, Teng Cui, Wen-jing Wang, Dao-min Tang, Rui-ming Zhang, Chen Zhang, Hui-yun Wang, Qiang Ren
Summary: Chondroitin sulfate (CCS) extracted from discarded codfish bones showed anticoagulant activity and potential as a dietary fiber supplement to prevent thrombosis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Cell Biology
Amrita Basu, Neil G. Patel, Elijah D. Nicholson, Ryan J. Weiss
Summary: Glycosaminoglycans (GAGs) are long polysaccharides found on the cell surface and extracellular matrix of animal cells, and play important roles in cellular processes and diseases. GAGs are complex molecules with extensive structural and functional heterogeneity, and their biosynthesis is a non-template driven process involving a group of enzymes. Due to their key roles in cell homeostasis and disease, targeting the assembly and function of GAGs is of great interest as a therapeutic approach.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Weijiao Zhang, Ruirui Xu, Xuerong Jin, Yang Wang, Litao Hu, Tianmeng Zhang, Guocheng Du, Zhen Kang
Summary: In this study, enzymatic strategies for producing homogeneous chondroitin oligosaccharides (o-CHs) and its sulfate derivatives from microbial sourced chondroitin were reported. The enzymatic approaches showed high conversion rates and pave the way for the study of structure-activity relationship and applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Medicine, Research & Experimental
Wei Zhang, Jikang Han, Zhiwen Jiang, Yanfei Peng, Xiaorui Sun, Baoqin Han
Summary: In this study, CS oligosaccharides (CSOs) were enzymatically prepared and their therapeutic potential for postmenopausal osteoporosis was explored. The results showed that CS and CSOs could effectively alleviate osteoporosis induced by ovariectomy in rats. CSOs exhibited a more significant alleviating effect compared to CS. These findings suggest that CSOs have great potential as daily interventions for delaying the progression of postmenopausal osteoporosis.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Chemistry, Medicinal
Nadezhda E. Ustyuzhanina, Natalia Yu. Anisimova, Maria I. Bilan, Fedor V. Donenko, Galina E. Morozevich, Dmitriy V. Yashunskiy, Anatolii I. Usov, Nara G. Siminyan, Kirill I. Kirgisov, Svetlana R. Varfolomeeva, Mikhail V. Kiselevskiy, Nikolay E. Nifantiev
Summary: Polysaccharides extracted from fish and sea cucumber were found to stimulate bone marrow release of blood cells and have a significant effect on immunosuppressed mice, promoting proliferative activity of hematopoietic cells and restoration of spleen function. Further research on oligosaccharides as potential drugs for prophylaxis and therapy of hematopoiesis inhibition post chemotherapy and transplantation is warranted.
Article
Chemistry, Applied
Ronghua Yin, Ying Pan, Ying Cai, Fan Yang, Na Gao, Dilihumaer Ruzemaimaiti, Jinhua Zhao
Summary: In this study, the physicochemical properties and derivatives of Fucosylated chondroitin sulfate from sea cucumber Ludwigothurea grisea (FCSLg) were analyzed to decipher its structure. Oligosaccharides analysis revealed the presence of mono-fucose and heterodisaccharide branches in FCSLg, with the di-fucosyl branch being determined as D-GalNAcR1-(alpha 1,2)-LFucR2. The diverse sulfation patterns in the branches complicated the structure. This study provides a new understanding of FCSLg and offers a reliable method for deciphering FCS with complex branches.
CARBOHYDRATE POLYMERS
(2022)
Review
Biochemistry & Molecular Biology
Aikaterini Berdiaki, Monica Neagu, Eirini-Maria Giatagana, Andrey Kuskov, Aristidis M. Tsatsakis, George N. Tzanakakis, Dragana Nikitovic
Summary: The tumor microenvironment (TME) is composed of various cells and extracellular matrix components. Changes in the structure and sulfation pattern of GAGs are associated with cancer progression. GAGs and GAG-based molecules show promise as potential effectors for anticancer therapy due to their unique properties.
Article
Chemistry, Applied
Pi-Xian Gong, Yan-Chao Wu, Xiao Chen, Ze-Lin Zhou, Xi Chen, Hui-Jing Li
Summary: Fucosylated chondroitin sulfate from the sea cucumber Holothuria fuscogilva (FCShf) possesses immunoregulatory activities and promotes cell proliferation. The molecular weight of FCShf significantly influences its activity, and FCShf activates cells via the TLR-2/4-NF-kappa B pathway.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Applied
Pi-Xian Gong, Qin-Ying Li, Yan-Chao Wu, Wen-Yu Lu, Jun Zeng, Hui-Jing Li
Summary: A fucosylated chondroitin sulfate (FCSsj) isolated from the body wall of sea cucumber Stichopus japonicus showed significant antidiabetic activity by promoting glucose uptake and consumption, alleviating cell damage, and promoting glycogen synthesis in vitro.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Applied
Shuxin Jiang, Huanan Yin, Xiaohui Qi, Weiguo Song, Weiwei Shi, Jiaojiao Mou, Jie Yang
Summary: Fucosylated chondroitin sulfate from Stichopus chloronotus has immune activation effects on RAW 264.7 macrophages, promoting cell activity and cytokine production. This potentially promising immunomodulator may improve macrophage function through NF-kappa B pathway.
CARBOHYDRATE POLYMERS
(2021)
Article
Hematology
Chuang Xiao, Longyan Zhao, Na Gao, Mingyi Wu, Jinhua Zhao
THROMBOSIS AND HAEMOSTASIS
(2019)
Review
Hematology
Lisha Lin, Longyan Zhao, Na Gao, Ronghua Yin, Sujuan Li, Huifang Sun, Lutan Zhou, Guiling Zhao, Steven W. Purcell, Jinhua Zhao
Article
Chemistry, Organic
Shuai Meng, Wenhe Zhong, Wang Yao, Zhongjun Li
Article
Chemistry, Organic
Pan Wang, Yidian Mo, Xiaoyu Cui, Xuyang Ding, Xiao Zhang, Zhongjun Li
Article
Hematology
Lutan Zhou, Na Gao, Huifang Sun, Chuang Xiao, Lian Yang, Lisha Lin, Ronghua Yin, Zi Li, Hongbin Zhang, Xu Ji, Jinhua Zhao
THROMBOSIS AND HAEMOSTASIS
(2020)
Article
Chemistry, Applied
Hui Mao, Ying Cai, Shanni Li, Huifang Sun, Lisha Lin, Ying Pan, Wenjiao Yang, Zhicheng He, Ru Chen, Lutan Zhou, Weili Wang, Ronghua Yin, Jinhua Zhao
CARBOHYDRATE POLYMERS
(2020)
Article
Cell Biology
Lisha Lin, Lian Yang, Jun Chen, Lutan Zhou, Sujuan Li, Na Gao, Jinhua Zhao
Summary: Fucosylated glycosaminoglycan (FG) extracted from sea cucumbers has anticoagulant effects, but also carries risks of platelet aggregation and activation. Research found that the impact of FG on human platelet aggregation depends on its sulfation pattern and molecular weight; TaFG induced platelet aggregation mediated by PI3K requires platelet secretion for amplification.
Article
Chemistry, Medicinal
Huifang Sun, Na Gao, Lin Ren, Shuang Liu, Lisha Lin, Wenqi Zheng, Lutan Zhou, Ronghua Yin, Jinhua Zhao
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Ronghua Yin, Lutan Zhou, Na Gao, Lisha Lin, Huifang Sun, Dingyuan Chen, Ying Cai, Zhili Zuo, Kaifeng Hu, Shengxiong Huang, Jikai Liu, Jinhua Zhao
Summary: This study reports a GAG with galactose-fucose branches from Thelenota ananas, demonstrating anticoagulant properties and solving the long-standing question of disaccharide-branched GAGs in Holothuroidea, offering new opportunities for developing safer anticoagulants.
Article
Chemistry, Applied
Xiaomei Li, Shanni Li, Jie Liu, Lisha Lin, Huifang Sun, Wenjiao Yang, Ying Cai, Na Gao, Lutan Zhou, Hongbo Qin, Ronghua Yin, Jinhua Zhao
Summary: Marine sulfated polysaccharides from sea cucumber Stichopus herrmanni and its depolymerized products showed significant anticoagulant activities due to their structures. The peroxide depolymerization process involved cleavage of glycosidic bonds and oxidative modification of reducing end of sugar residues, with the molecular size playing a role in their potency.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Applied
Junyin Zhang, Hulan Chen, Lan Luo, Zhipeng Zhou, Yingxiang Wang, Tianyu Gao, Lian Yang, Teng Peng, Mingyi Wu
Summary: Two new polysaccharides (a fructan and a galactan) were purified from Polygonatum cyrtonema rhizome, which showed prebiotic activity by promoting the growth of Bifidobacterium and Lactobacillus strains, indicating their potential application as functional ingredients in food products.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Medicinal
Lisha Lin, Sujuan Li, Na Gao, Weili Wang, Taocui Zhang, Lian Yang, Xingzhi Yang, Dan Luo, Xu Ji, Jinhua Zhao
Summary: The study found that native FG may cause significant cardiovascular and respiratory dysfunction, even lethality in rats, while its depolymerized products had no obvious side effects. The main toxicity of native FG is attributed to pulmonary microvessel embolism, while depolymerized FG can safely target iXase for anticoagulation.
Article
Chemistry, Applied
Shanni Li, Wei Zhong, Ying Pan, Lisha Lin, Ying Cai, Hui Mao, Taocui Zhang, Sujuan Li, Ru Chen, Lutan Zhou, Weili Wang, Qinghua Cui, Ronghua Yin, Shengxiong Huang, Jinhua Zhao
Summary: In this study, glycosaminoglycan HnFG was extracted from sea cucumber Holothuria nobilis and its precise structure was determined, which exhibited potent activities in prolonging the activated partial thrombin time (APTT) and inhibiting intrinsic coagulation Xase (iXase). The oligosaccharides, on the other hand, showed no obvious effects.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Medicinal
Zhipeng Zhou, Linlin Zhang, Xin Wu, Lan Luo, Jian Wu, Dan Xu, Mingyi Wu
Summary: Pentasaccharide analogues of fondaparinux were successfully synthesized using a simplified synthetic method, which showed stronger anticoagulant activities and longer-lasting antifactor Xa activities compared to fondaparinux.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Yuebo Wu, Zhipeng Zhou, Lan Luo, Maixian Tao, Xiao Chang, Lian Yang, Xingyong Huang, Lin Hu, Mingyi Wu
CARBOHYDRATE POLYMERS
(2020)
