Article
Optics
Gwinky G. K. Yip, Michelle C. K. Lo, Wenwei Yan, Kelvin C. M. Lee, Queenie T. K. Lai, Kenneth K. Y. Wong, Kevin K. Tsia
Summary: The new high-speed quantitative phase imaging (QPI) technique presented in this study combines time-interleaved phase-gradient FACED image detection, enabling fast imaging and cell analysis. Integration with microfluidic technology allows for high-speed, high-resolution imaging of cell phenotypes.
Article
Multidisciplinary Sciences
Ghazal Azarfar, Ebrahim Aboualizadeh, Simona Ratti, Camilla Olivieri, Alessandra Norici, Michael J. Nasse, Mario Giordano, Carol J. Hirschmugl
Summary: This study utilized mid-infrared spectroscopy to investigate individual Thalassiosira weissflogii cells in water, revealing significant compositional changes in organic pools within the algal cells in response to elevated CO2 concentrations.
SCIENTIFIC REPORTS
(2021)
Article
Optics
Jianan Fang, Kun Huang, E. Wu, Ming Yan, Heping Zeng
Summary: This article introduces an infrared 3D imaging system capable of detecting three-dimensional structure and reflectivity information. The system operates at single-photon sensitivity and femtosecond timing resolution, enabling imaging under low-light conditions. By nonlinear frequency upconversion and recording with a silicon camera, the presented system achieves high lateral and depth resolutions for 3D reconstruction. Additionally, a numerical denoiser based on spatiotemporal correlation allows the retrieval of object profile and reflectivity even with a detected flux below 0.05 photons/pixel/second. This infrared 3D imager features high detection sensitivity, precise timing resolution, and wide-field operation, offering new possibilities in life and material sciences.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Igor Buzalewicz, Agnieszka Ulatowska-Jarza, Aleksandra Kaczorowska, Marlena Gasior-Glogowska, Halina Podbielska, Magdalena Karwanska, Alina Wieliczko, Anna K. Matczuk, Katarzyna Kowal, Marta Kopaczynska
Summary: The article introduces a new method for investigating changes in living cells and quantitatively analyzing the process of bacteria photodynamic inactivation. The use of digital holographic tomography (DHT) allows real-time characterization of subcellular structures, biophysical processes, and induced local changes in intracellular density in a label-free manner and at sub-micrometer spatial resolution, which was confirmed by confocal microscopy and statistical analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Nicola Caporaso, Martin B. Whitworth, Ian D. Fisk
Summary: The study found that hyperspectral imaging technology can accurately and rapidly predict fat content at the single cocoa bean level, even from scans of unshelled beans. The estimation of fat content is beneficial for the food industry in quality control and obtaining more consistent raw materials.
Article
Multidisciplinary Sciences
Yuying Tan, Haonan Lin, Ji-Xin Cheng
Summary: This study demonstrates a high-content hyperspectral stimulated Raman scattering (h(2)SRS) imaging approach that allows for simultaneous mapping of multiple biomolecules at the single-cell level, revealing chemotherapy-induced metabolic reprogramming and unique metabolic features of chemoresistance. This approach could aid in the discovery of therapeutic targets to combat chemoresistance.
Article
Chemistry, Analytical
Cassio Lima, Howbeer Muhamadali, Yun Xu, Mustafa Kansiz, Royston Goodacre
Summary: This study demonstrates the use of infrared spectroscopy to track the uptake of stable isotope-labeled compounds by bacteria at the single-cell level, providing insights into microbial systems. The development of a new far-field infrared imaging technique, optical photothermal infrared (O-PTIR) spectroscopy, allows for monitoring the metabolic activity of individual bacteria and predicting isotopic ratios simultaneously using spectral signatures.
ANALYTICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Kun Li, Shaoli Duan, Lingling Pang, Weilai Li, Zhixiong Yang, Yaohang Hu, Chunchao Yu
Summary: Gas detection technologies have significant benefits in environmental monitoring, public safety, and safe production. Infrared imaging can be used to identify the type of gas from a distance, enabling long-distance and non-contact detection in safety production and hazardous chemical accident rescue. A gas detection system based on multispectral infrared imaging was developed, which can detect various gases and determine their types by separating infrared radiation. The system includes an imaging optical system, uncooled focal plane detector, filter controller, and data gathering and processing system. It has a resolution of 640 x 512 and a working band of 6.5 to 15 μm. The system successfully detected pollutants such as ammonia, sulfur hexafluoride, methane, sulfur dioxide, and dimethyl methyl phosphonate in real-time, with ammonia clouds detected at a distance of 1124.5 m.
Article
Optics
Daniele Pirone, Daniele Sirico, Lisa Miccio, Vittorio Bianco, Martina Mugnano, Danila del Giudice, Gianandrea Pasquinelli, Sabrina Valente, Silvia Lemma, Luisa Iommarini, Ivana Kurelac, Pasquale Memmolo, Pietro Ferraro
Summary: The latest discoveries in the biochemical field emphasize the increasing importance of lipid droplets (LDs) in cellular regulatory mechanisms. Traditional methods like fluorescence microscopy and transmission electron microscopy only provide 2D images and partial measurements, limiting analysis of large cell numbers. We demonstrate for the first time the capability of 3D visualization and full LD characterization using a tomographic phase-contrast flow-cytometer, providing more in-depth understanding and new discoveries on the correlation between LDs and cellular functions.
OPTO-ELECTRONIC ADVANCES
(2023)
Review
Chemistry, Analytical
Vicky Mudeng, Gelan Ayana, Sung-Uk Zhang, Se-woon Choe
Summary: Diffuse optical tomography is a cost-effective and preferred imaging modality that uses near-infrared light to assess soft tissue, such as breast and prostate, for cancer screening. Despite numerous studies, there is currently no consolidated review summarizing the application of near-infrared technology in cancer imaging and therapy.
Article
Multidisciplinary Sciences
Yuning Guo, Baowen Li, Xiaobo Yin
Summary: This study proposes a dual-compressed photoacoustic imaging method with single-pixel detection, which enables high-efficiency surface tomography with 3D spatial resolution unlimited by the acoustics, revealing the potential for dynamic imaging in a scattering medium.
NATIONAL SCIENCE REVIEW
(2023)
Article
Immunology
Chuan Yan, Qiqi Yang, Songfa Zhang, David G. Millar, Eric J. Alpert, Daniel Do, Alexandra Veloso, Dalton C. Brunson, Benjamin J. Drapkin, Marcello Stanzione, Irene Scarfo, John C. Moore, Sowmya Iyer, Qian Qin, Yun Wei, Karin M. McCarthy, John F. Rawls, Nick J. Dyson, Mark Cobbold, Marcela Maus, David M. Langenau
Summary: This study utilized optically clear immunocompromised zebrafish models engrafted with fluorescent-labeled human cancers and various T cell immunotherapies to visualize T cell immune responses at single-cell resolution in real time. Differences in kinetics of T cell infiltration, tumor cell engagement, and killing were identified among the immunotherapies, leading to early endpoint analysis for predicting therapy responses. EGFR-targeted immunotherapies were established as a powerful approach to eradicate rhabdomyosarcoma muscle cancers, suggesting the potential of assessing a broader range of T cell immunotherapies in this disease.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Biochemical Research Methods
Jiang Qian, Yadi Wang, Xueling Li, Junhong Lu
Summary: The stem cell microenvironment is crucial for regulating cell proliferation and differentiation. This study utilizes synchrotron radiation-based Fourier transform infrared microspectroscopy to examine the combined effects of physical and chemical factors on stem cell differentiation at the single-cell level. The results demonstrate the distinct effects of low-concentration lithium and Wnt5a on human mesenchymal stem cells in a polyvinyl alcohol (PVA) hydrogel, highlighting the important role of niche signals in the Wnt pathway. These findings emphasize the significance of the microenvironment in chemical-induced effects on stem cell differentiation and provide a label-free, noninvasive method to identify niche function in stem cell biology.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Optics
Margie P. Olbinado, David M. Paganin, Yin Cheng, Alexander Rack
Summary: Advances in X-ray imaging have led to breakthroughs in various fields, but achieving high spatial resolution while reducing radiation dose remains challenging. Ghost imaging shows promise in reducing dose and achieving high resolution, but current protocols are limited in achieving phase contrast and image quality.
Review
Biochemical Research Methods
Julen Ariztia, Kathleen Solmont, Nadia Pellegrini Moise, Simon Specklin, Marie Pierre Heck, Sandrine Lamande-Langle, Bertrand Kuhnast
Summary: Molecular imaging is a biomedical research discipline that allows observation and quantification of biomarkers and biological processes. Combining multimodality imaging techniques offers unprecedented tools for human health. In this review, we focus on the combination of positron emission tomography and fluorescence imaging and summarize the chemical synthesis strategies for dual imaging agents.
BIOCONJUGATE CHEMISTRY
(2022)
Correction
Chemistry, Analytical
Adrian Cernescu, Michal Szuwarzynski, Urszula Kwolek, Pawel Wydro, Mariusz Kepczynski, Szczepan Zapotoczny, Maria Nowakowska, Luca Quaroni
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Lucyano J. A. Macedo, Fernando P. Rodrigues, Ayaz Hassan, Leandro N. C. Maximo, Fabio Zobi, Roberto S. da Silva, Frank N. Crespilho
Summary: Recent emergence of FTIR spectromicroscopy (micro-FTIR) as a dynamic spectroscopy for imaging to study biological chemistry has opened new possibilities for investigating in situ drug release, redox chemistry effects on biological molecules, DNA and drug interactions, membrane dynamics, and redox reactions with proteins at the single cell level. Micro-FTIR applied to metallodrugs has been playing an important role since the last decade because of its great potential to achieve more robust and controlled pharmacological effects against several diseases, including cancer. An important aspect in the development of these drugs is to understand their cellular properties, such as uptake, accumulation, activity, and toxicity. The review highlights both the potential application of micro-FTIR and its importance for studying metal-based drugs, emphasizing the perspectives of chemistry of living cells and the significance of bioimaging for localization of cellular processes to understand the mechanism of action.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Multidisciplinary
Kevin Schindler, Fabio Zobi
Summary: Luminescent rhenium complexes are of growing scientific interest for catalytic, diagnostic, and therapeutic applications, with a focus on their photophysical and photochemical properties. The choice of ligands and strategies to exploit the potential of these molecules in medicinal applications are crucial. Their applications include being used as luminescent cellular probes and developing photoactive carbon monoxide-releasing molecules for anticancer and antimicrobial purposes.
Review
Biochemistry & Molecular Biology
Kevin Schindler, Fabio Zobi
Summary: Organometallic compounds of rhenium, particularly its tri- and dicarbonyl complexes, have shown promising potential as anticancer and antibiotic drugs due to their ease of preparation, stability, and unique photophysical and luminescent properties. This review focuses on discussing the structural variations and in vitro efficacy of these complexes in the past seven years, with an initial emphasis on tricarbonyl complexes and a later discussion on the future prospects of dicarbonyl species in this field.
Article
Multidisciplinary Sciences
Hagir B. Suliman, Zachary Healy, Fabio Zobi, Bryan D. Kraft, Karen Welty-Wolf, Joshua Smith, Christina Barkauskas, Claude A. Piantadosi
Summary: The preclinical model of bleomycin-induced lung fibrosis is useful to study mechanisms related to human pulmonary fibrosis. The study found that HO-1/NRF-1 signaling protects lung AT2 cells against injury and fibrosis by maintaining mitochondrial health and suppressing the TGF beta 1 pathway.
Article
Geochemistry & Geophysics
Anja Allabar, Patricia L. Petri, Dennis Eul, Marcus Nowak
Summary: In this study, the H2O solubility in peralkaline haplogranitic melts has been experimentally determined as a function of pressure, temperature, and excess alkali content. A new empirical model was established to predict the H2O solubility based on the excess alkali content, and it was tested with literature data, showing good agreement between predicted and observed H2O solubility.
CONTRIBUTIONS TO MINERALOGY AND PETROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sara Nasiri Sovari, Taryn M. Golding, Mziyanda Mbaba, Roxanne Mohunlal, Timothy J. Egan, Gregory S. Smith, Fabio Zobi
Summary: A small library of aminoquinoline and imidazolopiperidine (IMP)-based ligands showed potent inhibitory activities against chloroquine-sensitive (CQS) and multidrug-resistant (MDR) strains of P. falciparum. The compounds may act through hemozoin inhibition and the quinoline-based compounds exhibited higher binding affinities than the reference drug chloroquine.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Chemistry, Medicinal
Kevin Schindler, Youri Cortat, Miroslava Nedyalkova, Aurelien Crochet, Marco Lattuada, Aleksandar Pavic, Fabio Zobi
Summary: Antimicrobial resistance poses a significant threat to human health and has a major impact on the global economy. Metal complexes, including rhenium complexes, have untapped potential as antimicrobial agents. This study investigates the antimicrobial potential of rhenium di- and tricarbonyl diimine complexes and identifies two potential targets for these compounds. The findings have implications for the future design of rhenium tricarbonyl diimine-based antibiotics.
Article
Chemistry, Organic
Fatlinda Rahmani, Aurelien Crochet, Fabio Zobi
Summary: In this study, (E)-3-(2-(4-methylthiazol-2-yl)hydrazineylidene)chromane-2,4-dione was synthesized for the first time and characterized using various spectroscopic techniques. The chemical structure and isomeric configuration of the molecule were confirmed by single-crystal X-ray diffraction.
Article
Infectious Diseases
Youri Cortat, Miroslava Nedyalkova, Kevin Schindler, Parth Kadakia, Gozde Demirci, Sara Nasiri Sovari, Aurelien Crochet, Stefan Salentinig, Marco Lattuada, Olimpia Mamula Steiner, Fabio Zobi
Summary: In the context of the global health issue caused by antimicrobial resistance (AMR), the need for novel antimicrobial agents is urgent. Inorganic and organometallic complexes show potential as untapped sources of antibiotics. This study used a computer-aided drug design approach to synthesize and evaluate fac-Re(I) tricarbonyl complexes as antibacterial agents, with promising results.
Article
Chemistry, Inorganic & Nuclear
Kevin Schindler, Justine Horner, Gozde Demirci, Youri Cortat, Aurelien Crochet, Olimpia Mamula Steiner, Fabio Zobi
Summary: Cancer is still a leading cause of death worldwide, and the interest in organometallic complexes as anticancer drug candidates is crucial. Rhenium dicarbonyl complexes have been given less attention compared to tricarbonyl complexes. This study tested various rhenium dicarbonyl complexes in vitro against different cancer cell lines and found that some compounds showed good activity against specific cancer lines but were also toxic to healthy cells.
Review
Pharmacology & Pharmacy
Youri Cortat, Fabio Zobi
Summary: This review discusses the research work on coordination derivatives of antifungal azoles (AAs) synthesized for medical purposes, highlighting the most promising compounds and emphasizing the efficiency of AAs and their metallic species.
Article
Mineralogy
Patricia Louisa Marks, Anja Allabar, Marcus Nowak
Summary: The injection of mafic magma into a hydrous felsic magma chamber can trigger bimodal explosive volcanism. By conducting decompression experiments, researchers investigated the interaction and degassing behavior of hydrous rhyolitic melt in contact with hydrous basaltic melt. The experiments showed that vesiculated glass samples were formed during decompression, and a hybrid composition zone developed between the rhyolitic and basaltic melts. The findings suggest that convective magma ascent driven by the injection of basaltic magma can lead to enhanced vesicle formation and efficient degassing, potentially triggering explosive volcanic eruptions.
EUROPEAN JOURNAL OF MINERALOGY
(2023)
Article
Chemistry, Inorganic & Nuclear
Sara Nasiri Sovari, Isabelle Kolly, Kevin Schindler, Ana Djuric, Tatjana Srdic-Rajic, Aurelien Crochet, Aleksandar Pavic, Fabio Zobi
Summary: This study reports the synthesis, characterization, and in vivo evaluation of a series of rhenium tricarbonyl complexes containing 5- and 6-(halomethyl)-2,2'-bipyridine ligands. The aim was to investigate the role of a reactive halomethyl substituent on the diimine ligand system in the design of effective and non-toxic anticancer agents. Compounds capable of undergoing ligand-based alkylating reactions exhibited significant antiproliferative activity against colorectal and pancreatic cell lines. One specific compound (5-(chloromethyl)-2,2'-bipyridine derivative) displayed significant inhibition of pancreatic tumor growth in vivo in zebrafish-Panc-1 xenografts at a concentration of 8 μM, lower than its in vitro IC50 values, and also inhibited in vivo cancer cell dissemination.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Multidisciplinary
Kevin Schindler, Aurelien Crochet, Fabio Zobi
Summary: The article introduces new synthetic routes to produce aerobically stable and substitutionally labile alpha-diimine rhenium(i) dicarbonyl complexes. These complexes can be prepared in high yield and exhibit different reactions in various solvents, providing a convenient entry into the chemistry of the core.
