Article
Biochemistry & Molecular Biology
Tianyi Yu, Marzhana Omarova, Meng Zhang, Istiak Hossain, Jianqiang Chen, Omead Darvish, Vijay T. John, Donghui Zhang
Summary: The cellular functions of membrane proteins are closely related to their structures and aggregation states. We have investigated the fragmentation of synthetic liposomes using hydrophobe-containing polypeptoids (HCPs). Our findings demonstrate that HCPs can effectively induce the fragmentation of liposomes into colloidally stable nanoscale HCP-lipid complexes, and have the potential for novel macromolecular surfactants for membrane protein extraction.
Article
Pharmacology & Pharmacy
Dumei Ma, Libo Zhang, Yingwu Yin, Yuxing Gao, Qian Wang
Summary: This study investigated in detail the interactions between cycloalkyl phosphorus heterocycles and OleT(SA), revealing that hydrogen bonding and van der Waals forces play major roles in the interaction. The penetration of the cycloalkyl phosphorus heterocycles into the enzyme greatly affects the enzymatic activity of OleT(SA). This study provides an effective way to modulate the activity of cytochrome P450 enzymes using phosphorus heterocyclic compounds.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2021)
Article
Physics, Multidisciplinary
M. Maekawa, A. Miyashita, S. Sakai, S. Li, S. Entani, A. Kawasuso, Y. Sakuraba
Summary: By using spin-polarized positrons, researchers studied the surface density of states of various materials and found characteristic negative spin polarizations on Ni and Co surfaces, which disappeared upon deposition of graphene. The surface properties of CFGG and CMS exhibited weak spin polarizations, suggesting that the expected half-metallicity of these bulk states was not present at the surfaces.
PHYSICAL REVIEW LETTERS
(2021)
Article
Biotechnology & Applied Microbiology
Ru-Yi Ye, Juan Song, Zhi-Jun Zhang, Hui-Lei Yu
Summary: CYP120As are unique non-membrane P450 enzymes that play a significant role in the biodegradation of retinoids, with promising catalytic potential.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Yasukazu Hirao, Yoko Daifuku, Keiji Ihara, Takashi Kubo
Summary: The synthesis of phenalenyl-endcapped [5]cumulene as a cumulene-based singlet biradical and the spin correlation changes of one-dimensional aggregates are discussed. The introduction of bulky substituents into the appropriate positions led to the formation of a one-dimensional chain assembly, with increased bond length alternation of the cumulene chain and improved overlapping of the phenalenyl rings at lower temperatures. Variable-temperature Raman spectroscopy and magnetic susceptibility measurements revealed the decoupling of a localized spin pair at low temperatures, and the emergence of a continuum spin system involving intra- and intermolecular spin-spin interactions in the one-dimensional chain.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Optics
A. A. Fomin, M. Yu Petrov, I. I. Ryzhov, G. G. Kozlov, V. S. Zapasskii, M. M. Glazov
Summary: This study theoretically and experimentally investigates fluctuations of high spin beyond the linear response regime in high power density probe fields, revealing significant modifications to the spin noise spectra. The research predicts and demonstrates effects related to the interplay of high spin and perturbations, such as strong sensitivity of the spin noise spectra to the orientation of the probe polarization plane and magnetic field direction, appearance of high harmonics of the Larmor frequency in the spin noise, and the fine structure of Larmor peaks. The study showcases the capability of spin noise spectroscopy to explore nonlinear effects caused by the renormalization of spin states by strong electromagnetic fields.
Article
Multidisciplinary Sciences
Laurentijn Tilleman, Kaat Rubben, Wim Van Criekinge, Dieter Deforce, Filip Van Nieuwerburgh
Summary: This study tested the effectiveness of Targeted Locus Amplification (TLA) combined with Illumina or Nanopore sequencing in accurate genotyping and phasing of pharmacogenes. The results showed limited nucleotide mapping on target and incomplete coverage of genes with both sequencing methods. However, the optimized primer pair for the CYP2D6 gene in the Nanopore dataset resulted in correct haplotypes, indicating the potential reliability of this method with patient-specific primer design and optimization.
SCIENTIFIC REPORTS
(2022)
Review
Biotechnology & Applied Microbiology
Lihong Jiang, Lei Huang, Jin Cai, Zhinan Xu, Jiazhang Lian
Summary: This article discusses the strategies for improving the expression and activity of eukaryotic P450s in yeast, followed by examples of P450s involved in biosynthetic pathway engineering.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Chemistry, Inorganic & Nuclear
Nidhi Awasthi, Rolly Yadav, Anamika Shukla, Devesh Kumar
Summary: This study investigates the hydroxylation of 4-nitrophenol by Cytochrome P450 metalloenzyme, revealing a single state reactivity pathway.
INORGANIC CHEMISTRY COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Freeborn Rwere, Sangchoul Im, Lucy Waskell
Summary: By altering the 140s FMN binding loop of CYPOR, the activity with cyt P450s can be affected, with the mutant activity primarily determined by its ability to reduce ferric cyt P450. The different behaviors of the mutants are mainly due to the altered structure and mobility of the loop.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physics, Multidisciplinary
Ran He, Dan Wang, Nannan Luo, Jiang Zeng, Ke-Qiu Chen, Li-Ming Tang
Summary: In this study, nonrelativistic spin-momentum coupling is predicted in two-dimensional materials. Twist operations in antiferromagnetic bilayers can induce spin splitting comparable to spin-orbit coupling, and generate a transverse spin current with a high charge-spin conversion ratio. These findings demonstrate the potential for achieving electrically controlled magnetism in materials without spin-orbit coupling.
PHYSICAL REVIEW LETTERS
(2023)
Article
Multidisciplinary Sciences
Lulu Ni, Ping Miao, Jian Jiang, Fang Wan, Jiangan Li, Min Ai, Lingzhong Kong, Su Tu
Summary: This study found that Glycyrrhiza promotes the metabolism of toxic components of Aconitum by upregulating the expression of CYP3A, reducing the content of heart failure-related markers, and improving the myocardial energy metabolism disorder, thus alleviating the development of congestive heart failure.
Article
Chemistry, Multidisciplinary
Martin Testa-Anta, Julia N. Majcherkiewicz, Kai Xu, Alejandro R. Goni, Veronica Salgueirino
Summary: In this study, nanocrystals of antiferromagnetic Cr2O3 are shown to exhibit peculiar lattice dynamics and exceptionally strong spin-phonon coupling via Raman spectroscopy. The coupling strength is governed by the competing antiferromagnetic and ferromagnetic interactions, and changes in the surface spin configuration also play a crucial role. Overall, this work demonstrates the size dependence of the interplay between crystalline and magnetic structures in 3D antiferromagnets and offers a route for spin-phonon coupling engineering at the nanoscale. Moreover, it shows the possibility of coupling phononic excitations with magnetization dynamics at room temperature, providing a highly prospective nanomaterial for novel magnonic device design.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Alecia R. Gee, Isobella S. J. Stone, Tegan P. Stockdale, Tara L. Pukala, James J. De Voss, Stephen G. Bell
Summary: We have achieved efficient oxidation reactions on unactivated carbon-hydrogen bonds by modifying the enzyme structure.
CHEMICAL COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Anex Jose, Andrew W. Schaefer, Antonio C. Roveda, Wesley J. Transue, Sylvia K. Choi, Ziqiao Ding, Robert B. Gennis, Edward Solomon
Summary: Heme-copper oxidases play a crucial role in cellular respiration by cleaving the oxygen-oxygen bond to form the key intermediate PM, which initiates proton pumping. Experimental evidence shows that the heme iron(IV)-oxo in PM is magnetically coupled to both a copper(II) and a cross-linked tyrosyl radical in the active site, providing new insight into the oxygen-oxygen bond cleavage and proton-pumping mechanisms.
Article
Chemistry, Multidisciplinary
Jeffrey J. Liu, Maxime A. Siegler, Kenneth D. Karlin, Pierre Moenne-Loccoz
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Chemistry, Inorganic & Nuclear
Alex M. Confer, Sinan Sabuncu, Maxime A. Siegler, Pierre Moenne-Loccoz, David P. Goldberg
INORGANIC CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Takahiro Hayashi, Mathieu Ligibel, Emine Sager, Moritz Voss, Jurg Hunziker, Kirsten Schroer, Radka Snajdrova, Rebecca Buller
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Chemistry, Multidisciplinary
Jesse B. Gordon, Avery C. Vilbert, Ida M. DiMucci, Samantha N. MacMillan, Kyle M. Lancaster, Pierre Moenne-Loccoz, David P. Goldberg
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Multidisciplinary
Jorge L. Martinez, Sean A. Lutz, Daniel M. Beagan, Xinfeng Gao, Maren Pink, Chun-Hsing Chen, Veronica Carta, Pierre Moenne-Loccoz, Jeremy M. Smith
ACS CENTRAL SCIENCE
(2020)
Article
Chemistry, Physical
Moritz Voss, Robin Kung, Takahiro Hayashi, Magdalena Jonczyk, Michael Niklaus, Hans Iding, Dennis Wetzl, Rebecca Buller
Summary: This study presents a multi-faceted screening procedure to capture ketoreductase (KRED) diversity from different sources. By establishing a library containing 51 KRED enzymes, it was found that 29 of them had never been described before, with 18 exhibiting anti-Prelog preference. Additionally, analysis of the library's catalytic activity towards a diverse ketone substrate set revealed broad substrate scope and complementing enantio-preference of the individual KREDs.
Article
Chemistry, Multidisciplinary
Kelsey R. Miller, Saborni Biswas, Andrew Jasniewski, Alec H. Follmer, Ankita Biswas, Therese Albert, Sinan Sabuncu, Emile L. Bominaar, Michael P. Hendrich, Pierre Moenne-Loccoz, A. S. Borovik
Summary: The research focuses on designing artificial proteins to achieve active sites with entatic states by simulating a controlled environment. Utilizing biotin-streptavidin technology, artificial di-Fe cores containing Fe centers have been successfully assembled, revealing the process of Fe center separation regulation by the protein host.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Mikail D. Levasseur, Shiksha Mantri, Takahiro Hayashi, Maria Reichenbach, Svenja Hehn, Ying Waeckerle-Men, Pal Johansen, Donald Hilvert
Summary: This study explores a new application method for protein nanocages, enabling precise binding of targeting immunoglobulins and intracellular uptake of related cargos. However, the potential drawback of this technology is the masking effect of serum antibodies, which may diminish the efficacy of the targeting antibodies.
ACS CHEMICAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Moritz Pott, Matthias Tinzl, Takahiro Hayashi, Yusuke Ota, Daniel Dunkelmann, Peer R. E. Mittl, Donald Hilvert
Summary: Changing the primary metal coordination sphere is an effective strategy for tuning metalloprotein properties, as demonstrated in this study. The variants with different histidine analogues showed distinct activities, impacting cyclopropanation, N-H insertion, and S-H insertion. The genetically encoded analogues provide valuable tools for exploring mechanisms and enabling new chemistries in enzymes with functional histidine residues.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Inorganic & Nuclear
Cayden X. Bullock, Cooper S. Jamieson, Pierre Moenne-Loccoz, Buck Taylor, Jordan A. M. Gonzalez, Ellie A. Draves, Louis Y. Kuo
Summary: A new class of dioxomolybdenum(VI) catalyst is used to achieve sulfide oxidation, with experiments and computational modeling supporting a hypothesized reaction mechanism.
INORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Aniruddha Dey, Jesse B. Gordon, Therese Albert, Sinan Sabuncu, Maxime A. Siegler, Samantha N. MacMillan, Kyle M. Lancaster, Pierre Moenne-Loccoz, David P. Goldberg
Summary: A new nonheme iron(II) complex was reported, which reacts with NO(g) to form a stable mononitrosyl complex. The mononitrosyl complex exhibits highly activated characteristics and can rapidly convert to N2O in the absence of an exogenous reductant.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Alecia T. Dent, Marley Brimberry, Therese Albert, William N. Lanzilotta, Pierre Moenne-Loccoz, Angela Wilks
Summary: Interactions between Y75H holo-HasAp and HasR inhibit the release of heme for signaling and uptake, as shown by spectroscopic characterization. The structural features and ligand field of Y75H holo-HasAp are consistent with intermediate values, supported by EPR spectrum and crystal structure analysis.
Article
Chemistry, Multidisciplinary
Jireh Joy D. Sacramento, Therese Albert, Maxime Siegler, Pierre Moenne-Loccoz, David P. Goldberg
Summary: A new ferrous corrole complex [Fe-II(ttppc)](-) has been shown to bind dioxygen to form Fe-O-2, exhibiting an O-16/18(2)-isotope sensitive n(O-O) stretch at 1128 cm(-1) and a single n(Fe-O-2) at 555 cm(-1). This complex can mediate the dioxygenation of an indole derivative and extract hydrogen atoms from activated O-H bonds.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Ravikumar Reddi, Kimberly Matulef, Erika Riederer, Pierre Moenne-Loccoz, Francis Valiyaveetil
Summary: Regulation of ion conduction through the pore of a K+ channel involves coordinated action of the activation gate and inactivation gate, with the W67F mutant of the KcsA channel showing reduced inactivation and enhanced activation rate. Structural studies provide insights into the allosteric pathway and highlight the importance of ion occupancy at the S2 site for channel inactivation.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Shiliang Tian, Ruixi Fan, Therese Albert, Rahul L. Khade, Huiguang Dai, Kevin A. Harnden, Parisa Hosseinzadeh, Jing Liu, Mark J. Nilges, Yong Zhang, Pierre Moenne-Loccoz, Yisong Guo, Yi Lu
Summary: This study successfully achieved stepwise nitrosylation of a mononuclear nonheme iron site in an engineered azurin under physiological conditions, controlled formation of {FeNO}(7) and {Fe(NO)(2)}(9) species by tuning the nitric oxide equivalents and reaction time. The potential formation of the elusive {FeNO}(8) species was inferred by EPR spectroscopy, while the conversion of {FeNO}(7) to {Fe(NO)(2)}(9) species was supported by various spectroscopic techniques.
