Article
Genetics & Heredity
Rongkang Li, Chong Lu, Xinji Li, Xuan Chen, Guocheng Huang, Zhenyu Wen, Hang Li, Lingzhi Tao, Yimin Hu, Zhengping Zhao, Zebo Chen, Yongqing Lai
Summary: In this study, six miRNAs were found to be significantly dysregulated in the serum of RCC patients compared to normal controls, and a four-miRNA panel was established to enhance diagnostic value.
FRONTIERS IN GENETICS
(2022)
Article
Pathology
Xuan Chen, Rongkang Li, Xinji Li, Xiqi Peng, Chunduo Zhang, Kaihao Liu, Guocheng Huang, Yongqing Lai
Summary: The study aimed to identify serum miRNAs as potential biomarkers for screening renal cell carcinoma, and found six miRNAs significantly differentially expressed in RCC patients compared to HCs. By combining these miRNAs, a four-microRNA panel was established with high diagnostic value. Bioinformatics analysis suggested potential involvement of these miRNAs in cancer processes. The study highlights the potential of the four-miRNA panel as a non-invasive biomarker for RCC screening.
PATHOLOGY RESEARCH AND PRACTICE
(2021)
Article
Oncology
Rongkang Li, Wenkang Chen, Chong Lu, Xinji Li, Xuan Chen, Guocheng Huang, Zhenyu Wen, Hang Li, Lingzhi Tao, Yimin Hu, Zhengping Zhao, Zebo Chen, Liangchao Ni, Yongqing Lai
Summary: This study found that the expression of miR-155-5p and miR-224-5p in serum was significantly upregulated in renal cell carcinoma (RCC) patients, while miR-1-3p, miR-124-3p, miR-129-5p, and miR-200b-3p were downregulated. A four-miRNA panel was constructed, which showed high diagnostic ability with a specificity of 93.67% and sensitivity of 75.61%. Bioinformatic analysis suggested CHL1, MPP5, and SORT1 as potential target genes of this panel. Survival analysis showed that miR-155-5p was significantly associated with the survival rate of RCC.
FRONTIERS IN ONCOLOGY
(2023)
Review
Oncology
Jose D. Debes, Pablo A. Romagnoli, Jhon Prieto, Marco Arrese, Angelo Z. Mattos, Andre Boonstra
Summary: Hepatocellular carcinoma (HCC) is a major cause of cancer death worldwide. Late diagnosis and limited treatment options contribute to the low survival rate of patients with HCC. Currently, combining ultrasound with the biomarker AFP is a commonly used screening method, but its clinical utility and operator-dependence are controversial.
Article
Medicine, General & Internal
Sora Kim, Gwang Ha Kim, Su Jin Park, Chae Hwa Kwon, I Hoseok, Moon Won Lee, Bong Eun Lee
Summary: Exosomal miRNAs have the potential to serve as biomarkers for esophageal squamous cell carcinoma (ESCC). This study identified specific exosomal miRNAs that were differentially expressed in ESCC, and validated their expression levels in the plasma of healthy controls and ESCC patients. The results showed that plasma exosomal miR-205-5p, miR-429, and miR-375-3p could be potential biomarkers for the diagnosis of ESCC.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Oncology
Lei Shi, Mengheng Wang, Haiping Li, Pengtao You
Summary: ccRCC is the most common subtype of renal cell carcinoma with high mortality, significantly impacting patients' survival. miRNAs serve as effective diagnostic and prognostic biomarkers, as well as predictors of treatment drug efficacy, improving precision in clinical medication. Non-invasive detection of miRNAs or extracellular vesicles in body fluids shows advantages over tissue detection, offering convenience and repeatability for clinical diagnosis and prognosis of ccRCC.
CANCER MANAGEMENT AND RESEARCH
(2021)
Article
Oncology
Wangrui Liu, Chunguang Ma, Hong Xu, Lijun Wang, Wenhao Xu, Hailiang Zhang, Zhisu Wang, Jun Li, Ji Zhang, Xigao Liu, Shuai Zhao, Tao Wang
Summary: In this study, miR-184-5p was identified as a distinct prognostic biomarker for ccRCC and was found to regulate cell proliferation, invasion, and apoptosis by targeting NUS1.
Article
Environmental Sciences
Kexin Cao, Jingbing Zhang, Geng Wang, Xiaosheng Lin, Fulan Zhan, Kusheng Wu, Wei Tan, Hui Geng, Caixia Liu
Summary: Previous studies have explored the impacts of trace elements on human beings and their complex relationships with cancers. This study investigates the distribution of trace elements in different biological specimens and finds variations in concentrations between cancer patients and healthy individuals. Certain elements in blood are useful in assessing element exposure burdens and some elements tend to accumulate in tumors. This study provides further evidence for understanding the etiological mechanisms behind trace element-related diseases.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Multidisciplinary Sciences
Adele M. Alchahin, Shenglin Mei, Ioanna Tsea, Taghreed Hirz, Youmna Kfoury, Douglas Dahl, Chin-Lee Wu, Alexander O. Subtelny, Shulin Wu, David T. Scadden, John H. Shin, Philip J. Saylor, David B. Sykes, Peter V. Kharchenko, Ninib Baryawno
Summary: By analyzing ccRCC patient samples using single-cell transcriptomics, a distinct transcriptional signature predictive of metastatic potential and patient survival was identified. Potential therapeutic targets including the CXCL9/CXCL10-CXCR3 axis and the CD70-CD27 axis were highlighted.
NATURE COMMUNICATIONS
(2022)
Review
Oncology
Tim Nestler, Justine Schoch, Gazanfer Belge, Klaus-Peter Dieckmann
Summary: miR371 is a more sensitive and specific biomarker for testicular germ cell cancer than conventional serum markers. It shows potential for initial diagnosis, treatment monitoring, and follow-up. However, miR371 is not expressed in teratoma. Further studies are needed to determine its importance in detecting testicular tumor recurrences and residual tumors after chemotherapy.
Editorial Material
Oncology
Viktor Gruenwald, Walter J. Storkus
Summary: This collection focuses on the immunobiology of renal cell carcinoma (RCC), the impact of immunotherapy in treating RCC, and the identification of biomarkers related to patient outcomes and response to immunotherapy.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Aron Bartha, Zsuzsanna Darula, Gyoengyi Munkacsy, Eva Klement, Peter Nyirady, Balazs Gyorffy
Summary: This research used a proteotranscriptomic approach to differentiate normal and tumor tissues in clear cell renal cell carcinoma (ccRCC). Top genes over-expressed in ccRCC were identified using transcriptomic data of patients with malignant and normal tissue samples. Protein level analysis and mass spectrometry further validated the differential protein abundance of these genes. A minimal panel of proteins highly specific for ccRCC tissues was identified using transcriptomic and proteomic data.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Xianhui Liu, Weiyu Zhang, Huanrui Wang, Lin Zhu, Kexin Xu
Summary: ACADSB plays an important role in the diagnosis and prognosis of ccRCC. Its downregulation may promote tumorigenesis and tumor progression. The expression level of ACADSB can serve as a significant parameter to discriminate between normal and tumor tissues.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Zongjie Wei, Fan Zhang, Xin Ma, Weiyang He, Xin Gou, Xu Zhang, Yongpeng Xie
Summary: This study explored the prognostic value of preoperative red blood cell distribution width (RDW) in patients with metastatic renal cell carcinoma (mRCC). The results showed that higher RDW levels were associated with worse progression-free survival (PFS) and overall survival (OS). Multivariate analysis revealed that RDW was an independent risk factor for PFS and OS in mRCC patients after cytoreductive nephrectomy.
TRANSLATIONAL ONCOLOGY
(2022)
Article
Oncology
Manish Kohli, Winston Tan, Berengere Vire, Pierre Liaud, Melina Blairvacq, Frederic Berthier, Daniel Rouison, George Garnier, Lea Payen, Thierry Cousin, Dominique Joubert, Alexandre Prieur
Summary: Metastatic renal cell carcinoma (mRCC) is a significant portion of newly diagnosed kidney cancers, and better understanding of disease progression has led to the identification of hPG80 as a potential prognostic marker, which may improve current clinical prognostic models for mRCC patients.
Article
Immunology
Evangelos Andreakos, Laurent Abel, Donald C. Vinh, Elzbieta Kaja, Beth A. Drolet, Qian Zhang, Cliona O'Farrelly, Giuseppe Novelli, Carlos Rodriguez-Gallego, Filomeen Haerynck, Carolina Prando, Aurora Pujol, Helen C. Su, Jean-Laurent Casanova, Andras N. Spaan, Paul Bastard, Catherine M. Biggs, Benedetta Bigio, Bertrand Boisson, Alexandre Bolze, Anastasiia Bondarenko, Petter Brodin, Samya Chakravorty, John Christodoulou, Aurelie Cobat, Antonio Condino-Neto, Stefan N. Constantinescu, Hagit Baris Feldman, Jacques Fellay, Rabih Halwani, Emmanuelle Jouanguy, Yu-Lung Lau, Isabelle Meyts, Trine H. Mogensen, Satoshi Okada, Keisuke Okamoto, Tayfun Ozcelik, Qiang Pan-Hammarstrom, Anna M. Planas, Anne Puel, Lluis Quintana-Murci, Laurent Renia, Igor Resnick, Anna Sediva, Anna Shcherbina, Ondrej Slaby, Ivan Tancevski, Stuart E. Turvey, K. M. Furkan Uddin, Diederik van de Beek, Mayana Zatz, Pawel Zawadzki, Shen-Ying Zhang
Summary: SARS-CoV-2 infections can vary greatly between individuals, with some being asymptomatic while others experience life-threatening disease. While 20% of critical COVID-19 cases are due to inborn errors or autoantibodies, the genetic and immunological factors for resistance to infection itself remain unknown. Studies have shown that autosomal recessive deficiencies in certain genes or enzymes can confer resistance to diseases, suggesting a potential strategy for identifying individuals naturally resistant to SARS-CoV-2 infection.
Article
Pharmacology & Pharmacy
Martin Krkoska, Jana Nekvindova, Katerina Nevedelova, Veronika Zubanova, Lenka Radova, Jan Vondracek, Jarmila Herudkova, Ondrej Slaby, Igor Kiss, Lucia Bohovicova, Pavel Fabian, Zuzana Tylichova, Zdenek Kala, Petr Kysela, Lenka Ostrizkova, Vladimir Palicka, Alena Hyrslova Vaculova
Summary: The study revealed a potential link between miRNAs and downregulation of drug-metabolizing CYPs in HCC tumors, with miR-29c and miR-653 acting as efficient suppressors of CYP1A2 or AHR expression. This highlights a novel role of miR-653 and miR-29c in regulating CYPs involved in crucial biotransformation processes in liver, often deregulated during liver cancer progression.
PHARMACOLOGICAL REPORTS
(2022)
Article
Oncology
Michal Stanik, Alexander Poprach, Simona Littnerova, Ivo Capak, Marketa Hulova, Natalia Sebova, Radek Lakomy, Jiri Jarkovsky, Jan Dolezel
Summary: Aggressive surgical therapy is feasible for isolated retroperitoneal recurrence in renal cancer patients after radical nephrectomy, with more than half of the patients achieving long-term survival. Prognostic factors of poor outcome include an interval from nephrectomy to recurrence of less than 12 months, tumor grade 3-4, and female sex. Metastasis-directed therapy may prolong the time to initiation of systemic treatment in cases of disease progression.
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2022)
Article
Surgery
Tomas Novotny, Robert Staffa, Josef Tomandl, Tomas Krivka, Ondrej Slaby, Lubos Kubicek, Ernest Biros, Marie Tomandlova, Robert Vlachovsky, Jan Sponiar, Lenka Radova, Anna Konieczna
Summary: This study aimed to evaluate the value of L-lactate as a marker for early detection of postoperative intestinal ischemia. The results showed that measuring serum L-lactate levels at proper timepoints might help in the early diagnosis of postoperative intestinal ischemia after aortic surgery.
INTERNATIONAL JOURNAL OF SURGERY
(2022)
Article
Oncology
Tomas Hrbac, Alena Kopkova, Frantisek Siegl, Marek Vecera, Michaela Ruckova, Tomas Kazda, Radim Jancalek, Michal Hendrych, Marketa Hermanova, Vaclav Vybihal, Pavel Fadrus, Martin Smrcka, Filip Sokol, Vaclav Kubes, Radim Lipina, Ondrej Slaby, Leos Kren, Jiri Sana
Summary: HLA-E and HLA-F play an important role in GBM biology and could be used as diagnostic and prognostic biomarkers.
CANCER GENOMICS & PROTEOMICS
(2022)
Article
Medical Laboratory Technology
Julia Bohosova, Marek Kasik, Adela Kubickova, Karolina Trachtova, Michal Stanik, Alexandr Poprach, Ondrej Slaby
Summary: This study used next-generation sequencing to comprehensively analyze the expression profiles of lncRNAs in renal cell carcinoma patients, and identified several dysregulated lncRNAs associated with the disease. Among them, PVT1 was found to be significantly dysregulated in renal cell carcinoma and further experiments confirmed its effect on cell viability and migration. These results suggest that PVT1 not only has diagnostic potential, but also may serve as a potential target for the treatment of renal cell carcinoma.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Health Care Sciences & Services
Cecilia Pop-Bica, Cristina Alexandra Ciocan, Cornelia Braicu, Antonia Harangus, Marioara Simon, Andreea Nutu, Laura Ancuta Pop, Ondrej Slaby, Atanas G. Atanasov, Radu Pirlog, Nadim Al Hajjar, Ioana Berindan-Neagoe
Summary: This study investigated the genetic profile of lung cancer using TCGA datasets and NGS experiments, revealing differences in mutational landscape among histotypes. The findings suggest that certain genetic alterations may be correlated with patient survival rates.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Environmental Sciences
Dita Kadlcikova, Petra Musilova, Hana Hradska, Miluse Vozdova, Marketa Petrovova, Marek Svoboda, Jiri Rubes
Summary: The study investigated occupationally induced chromosomal damage in hospital personnel exposed to antineoplastic drugs and/or low doses of ionizing radiation. Both cytogenetic methods showed an increase in chromatid and chromosomal aberrations, as well as unstable aberrations, indicating recent exposure to genotoxic agents.
ARCHIVES OF ENVIRONMENTAL & OCCUPATIONAL HEALTH
(2023)
Article
Medical Laboratory Technology
Marie Tomandlova, Tomas Novotny, Robert Staffa, Jindra Smutna, Tomas Krivka, Peter Kruzliak, Ondrej Slaby, Lubos Kubicek, Robert Vlachovsky, Lenka Radova, Josef Tomandl
Summary: This study investigates the perioperative kinetics of D-lactate and ischemia-modified albumin (IMA) and their predictive value in early diagnosis of acute intestinal ischemia. The results suggest that serum levels of IMA and D-lactate can serve as influential predictive markers for postoperative intestinal ischemia, especially after 3 hours from complete declamping of vascular reconstruction.
CLINICAL BIOCHEMISTRY
(2023)
Article
Endocrinology & Metabolism
Jaroslav Juracek, Marie Madrzyk, Michal Stanik, Michaela Ruckova, Karolina Trachtova, Hana Malcikova, Eva Lzicarova, Dominik A. Barth, Martin Pichler, Ondrej Slaby
Summary: This study identified a miRNA expression pattern associated with disease aggressiveness in prostate cancer patients and established a prognostic score combining miRNA levels and serum PSA level that can distinguish between benign and aggressive prostate cancer.
Article
Oncology
Ivana Roskova, Marek Vecera, Lenka Radova, Karolina Trachtova, Frantisek Siegl, Marketa Hermanova, Michal Hendrych, Leos Kren, Vaclav Vybihal, Hana Valekova, Petra Kasparova, Ivana Kolouskova, Tomas Kazda, Ondrej Slaby, Radim Jancalek, Jiri Sana, Martin Smrcka
Summary: This study aimed to discover novel biomarkers for the origin of brain metastases (BMs) and develop a diagnostic classifier. The study used miRNA sequencing and RT-qPCR validation, and identified 6 miRNAs as diagnostic markers for BMs.
CANCER GENOMICS & PROTEOMICS
(2023)
Article
Genetics & Heredity
Katerina Slaba, Marta Jezova, Petra Pokorna, Hana Palova, Jana Tuckova, Jan Papez, Dagmar Prochazkova, Petr Jabandziev, Ondrej Slaby
Summary: Cardiac-urogenital syndrome is a rare syndrome associated with pathogenic variants in the MYRF gene, leading to loss of protein function. It is characterized by complex congenital cardiac defects, pulmonary hypoplasia, diaphragmatic hernia, and urogenital malformations. We report a case of two siblings with extensive developmental defects, who were found to have a novel splicing pathogenic variant in the MYRF gene. The presence of the identical de novo event in siblings suggests gonadal mosaicism, which mimics recessive inheritance. This is the first published case indicating gonadal mosaicism in familial cardiac-urogenital syndrome.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2023)
Article
Medicine, General & Internal
Michal Hendrych, Peter Solar, Marketa Hermanova, Ondrej Slaby, Hana Valekova, Marek Vecera, Alena Kopkova, Zdenek Mackerle, Tomas Kazda, Petr Pospisil, Radek Lakomy, Jan Chrastina, Jiri Sana, Radim Jancalek
Summary: This case report describes a 43-year-old female patient with frontal glioblastoma who developed intraspinal metastasis five months after surgery. Next-generation sequencing analysis revealed a higher number of single nucleotide variants in the metastatic sample compared to the primary tumor. Mutations in NF1, NOTCH3, and ARID1A were identified and may contribute to the invasiveness and metastatic potential of this particular glioblastoma case.
