Review
Chemistry, Analytical
Lili Zhang, Na Li, Shengshuang Chen, Xiqing Bian, Mohamed A. Farag, Yahui Ge, Jianbo Xiao, Jian-Lin Wu
Summary: Carboxyl-containing compounds (CCCs) are crucial for the human body and their determination in food is challenging. Derivatization LC-MS technique plays a significant role in overcoming these challenges in food chemistry. This review comprehensively discusses CCCs in terms of structures, biological functions, and food sources, highlighting the importance of derivatization LC-MS as a powerful analytical tool.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Aimin Wang, Yuting Fan, Jun Fu, Fengrui Song, Zhiqiang Liu, Shu Liu
Summary: This study proposed a new targeted metabolomics strategy using isoniazid derivatization combined with LC-MS/MS to simultaneously quantify 39 carboxyl-containing metabolites (CCMs) associated with Alzheimer's disease. The method showed high yields, accuracy, and stability, and has the potential for trace biological lineage analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Artemis Lioupi, Maria Marinaki, Christina Virgiliou, Olga Begou, Helen Gika, Ian Wilson, Georgios Theodoridis
Summary: Metabolomics is an interdisciplinary field with applications in various areas, involving the analysis of metabolites in pathways such as glycolysis, amino acid metabolism, and the Krebs cycle. The determination of polar metabolites poses major challenges for chromatographic analysis, and existing methods are unable to comprehensively profile the polar metabolome. This review summarizes current approaches, advances, and trends in using liquid chromatography/mass spectrometry to address the instrumental and intellectual challenges involved in mapping the polar metabolome.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Pascal Schorr, Caroline S. Stokes, Dietrich A. Volmer
Summary: The study optimized a one-pot double derivatization scheme for efficient separation and detection of vitamin D metabolites, overcoming challenges related to low ionization efficiency. However, limitations still exist, especially in the quantification of certain isomers.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Wei Li, Pei Zhang, Xiaoying Hou, Tian Tang, Siqi Li, Ruiqi Sun, Zunjian Zhang, Fengguo Xu
Summary: Modified metabolites play crucial roles in disease progression and diagnosis. A new LC-MS/MS method was developed for the simultaneous quantification of prototypes and their modified forms, with improved sensitivity and accuracy. The method was successfully applied to study lung adenocarcinoma cell lines.
ANALYTICA CHIMICA ACTA
(2022)
Article
Food Science & Technology
Elena Bergamasco, Gregorio Peron, Andrea Venerando, Shakil Ahmed Polash, Ravi Shukla, Stefania Sut, Stefano Dall'Acqua, Antonio Masi
Summary: This study provides a comprehensive characterization of sulfur-containing metabolites in asparagus using LC-MS and GC-MS. 80 S-containing compounds were identified and their distribution in different parts of the spears was assessed. The identified compounds are considered to contribute to the taste and odor properties of green and white asparagus.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemical Research Methods
Anastasia Alexandridou, Dietrich A. Volmer
Summary: This study investigated the long-term stability of various vitamin D-3 metabolites after storage and found that some derivatization reagents were more stable than others.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Suji Kim, Min Seo Lee, Mingyu Kim, Beom Jun Ko, Hye Suk Lee, Sooyeun Lee
Summary: This study aimed to develop a sensitive and accurate method for the simultaneous determination of endogenous gamma-hydroxybutyric acid (GHB) and its metabolites in human urine. LC-MS/MS was used to quantify the concentrations, and chemical derivatization with benzoyl chloride was employed to improve the sensitivity for certain compounds. Additionally, the effects of handling and storage conditions of urine samples on metabolite production and degradation were observed.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Tengfei Yuan
Summary: In this study, a sample-multiplexing LC-MS/MS method was developed and validated for the quantification of serum estrogens. The method demonstrated high accuracy and precision, and allowed simultaneous analysis of E1, E2, and E3.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yanping Lin, Krista Spiller, Radha Aras, Wenying Jian
Summary: A highly selective and sensitive UPLC-MS/MS assay was developed for quantitation of six catecholamines and their metabolites in rat and mouse striatum as pharmacodynamic biomarkers to support neuroscience and pharmaceutical research. The assay successfully measured the six analytes in striatum from mice treated with the neurotoxin MPTP and confirmed the metabolic pathway of neurotoxicity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Hsin-Yu Liao, Chin-Yi Wang, Ching-Hua Lee, Hsien-Li Kao, Wei-Kai Wu, Ching-Hua Kuo
Summary: A sensitive and effective LC-MS/MS method was developed to simultaneously quantify gut microbiota-related metabolites in human plasma. The study revealed a significant decrease in plasma butyrate levels in CVD patients, suggesting a potentially protective role in CVD.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Food Science & Technology
Xuan Zeng, Yuying Zheng, Yan He, Wei Peng, Weiwei Su
Summary: Naringin is a dietary flavonoid glycoside with multiple bioactivities. This study developed a rapid and sensitive method for simultaneous determination of ten flavonoid metabolites of naringin in rat urine. The results revealed the visible individual difference and low urinary recovery of flavonoid metabolites in the excretion of naringin, which may be helpful for further study to understand the in vivo behavior and action mechanism of naringin.
Article
Food Science & Technology
Fulya Tasci, Hale Secilmis Canbay, Mahmut Doganturk
Summary: The study found antibiotic residues in non-compliant milk samples, suggesting that not all milk producers adhere to good veterinary practices.
Article
Biochemical Research Methods
Anastasia Alexandridou, Pascal Schorr, Dietrich A. A. Volmer
Summary: The study compares the sensitivity and selectivity of different reagents for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of multiple vitamin D metabolites after chemical derivatization. Derivatization is commonly used to improve ionization efficiency and selectivity of vitamin D metabolites. The study investigated various reagents and found Amplifex to be the optimal derivatization reagent for multiple metabolite profiling. The study provides valuable information for vitamin D laboratories to choose the appropriate derivatization reagent for their applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Honoka Kaneko, Hiroki Matsuoka, Takayuki Ishige, Hironori Kobayashi, Tatsuya Higashi
Summary: This study developed an improved derivatization method combined with LC/ESI-MS/MS for the sensitive and specific quantification of serum/plasma E-2. The new method reduced the reaction time and interference peaks compared to the previous method, making it a sensitive and specific alternative for clinical analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Yaqi Zhang, Xiqing Bian, Guanyu Yan, Baoqing Sun, Wen Miao, Mingzheng Huang, Na Li, Jian-Lin Wu
Summary: In this study, a non-targeted LC-MS approach was used to identify 251 metabolites in Fruit of Rosa roxburghii Tratt (FRR). Differential compounds were identified to distinguish FRR from related species, and FRR exhibited higher biofunction potential. A quantitative LC-MS method was established to determine the contents of specific compounds, and it was found that FRR had higher contents compared to a related species. Furthermore, new ascorbic acid derivatives were discovered in FRR. These findings provide important information for the exploitation of FRR.
Article
Clinical Neurology
Xiqing Bian, Na Zhou, Yiran Zhao, Yichao Fang, Na Li, Xin Zhang, Xuan Wang, Yunxia Li, Jian-Lin Wu, Tingting Zhou
Summary: This study aimed to illustrate the metabolic mechanisms of depression and diagnose depression through biomarkers. The results showed that proline, 1-pyrroline-5-carboxylate (P5C), and glutamic acid may be potential biomarkers for depression. Additionally, serum samples could be an alternative diagnostic approach for depression.
PSYCHIATRY AND CLINICAL NEUROSCIENCES
(2023)
Article
Chemistry, Applied
Wen Miao, Xiaojing Liu, Na Li, Xiqing Bian, Yiran Zhao, Jing He, Tingting Zhou, Jian-Lin Wu
Summary: In this study, multiple liquid chromatography-mass spectrometry (LC-MS) metabolomics approaches were used to comprehensively characterize the components of Citrus aurantium L. Thirteen polymethoxyflavones (PMFs), 28 carbohydrates, and 18 carboxylic acids were identified for the first time in C. aurantium. A total of 147 compositions were characterized, including 92 that were reported for the first time. Geographical features and principal discriminative compounds were determined, and the biofunctions of C. aurantium from different regions were speculated.
Article
Environmental Sciences
Xuyang Chai, Luyao Wen, Yuanyuan Song, Xiaochong He, Jingxian Yue, Jianlin Wu, Xin Chen, Zongwei Cai, Zenghua Qi
Summary: This study investigates the key characteristics of cardiovascular risk in diet-induced obese mice and corresponding normal mice exposed to DEHP at environmentally relevant concentrations. The results suggest that the cardiovascular system of obese individuals is more susceptible to DEHP exposure. DEHP exposure induces gut microbial remodeling and alters arachidonic acid metabolism, which is associated with adverse cardiovascular events. The findings provide new insights into DEHP-induced cardiovascular damage in obese individuals and suggest the potential use of arachidonic acid as a modulator of gut microbiota to prevent related diseases.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Bin Zhang, Shaowei Dong, Jian Wang, Tuxiong Huang, Pan Zhao, Jing Xu, Dongcheng Liu, Li Fu, Lingwei Wang, Guangsuo Wang, Chang Zou
Summary: By using 3D printed patient derived xenograft models, this study identifies a NOTCH mutation as a predictor of response to EGFR-TKI therapy in LUAD. The NOTCH4(Delta L12_16) mutation increases the occurrence in EGFR-TKI-sensitive patients and sensitizes EGFR-TKI-resistant LUAD cells to the drug. This is mediated by reduced localization of NOTCH4 and down-regulation of HES1 expression. Inhibition of the NOTCH4-HES1 pathway can reverse EGFR-TKI resistance.
NATURE COMMUNICATIONS
(2023)
Article
Pharmacology & Pharmacy
Xiqing Bian, Yida Zhang, Na Li, Menglin Shi, Xiaolin Chen, Hui-Lu Zhang, Jie Liu, Jian-Lin Wu
Summary: Trace amines (TAs) are metabolically related to catecholamine and associated with cancer and neurological disorders. A sensitive method using diisopropyl phosphite coupled with chip two-dimensional liquid chromatography tandem triple-quadrupole mass spectrometry (LC-QQQ/MS) was developed to simultaneously determine TAs and associated metabolites. The results showed significantly altered TAs and associated metabolites in hepatoma cells after sorafenib treatment, indicating the potential for elucidating disease mechanisms and diagnosing diseases based on the physiological functions of TAs.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Review
Biochemistry & Molecular Biology
Ahmed Serag, Mohamed A. Salem, Shilin Gong, Jian-Lin Wu, Mohamed A. Farag
Summary: Plants interact with various organisms and face diverse aggressions. They have the ability to synthesize a wide array of chemical compounds, but only a small portion has been characterized for defense responses. Our knowledge of their biosynthetic pathways and functions is still incomplete. Recent progress has been made in phenylpropanoids and oxylipids metabolism. Advanced analysis methods, including different metabolomics approaches, imaging, flux analysis, and bioinformatics tools, are needed for monitoring plant metabolic reprogramming. Future developments are necessary to better understand intricate metabolic interactions in plants.
Article
Cell Biology
Ye Jin, Li Fu
Summary: Rationally designed and engineered bacteria have emerged as a unique and promising approach for cancer treatment. Researchers have successfully developed a short-lived bacterium, mp105, which is effective against various types of cancer and safe for intravenous administration. This bacterium combats cancer through direct oncolysis, depletion of tumor-associated macrophages, and elicitation of CD4+ T cell immunity.
CELL REPORTS MEDICINE
(2023)
Article
Oncology
Houxiang Jiang, Shaoxiang Wang, Ying Liu, Chaopan Zheng, Lipeng Chen, Kai Zheng, Zhenyu Xu, Yong Dai, Hongtao Jin, Zhiqiang Cheng, Chang Zou, Li Fu, Kaisheng Liu, Xiaoshi Ma
Summary: This study identified EFNA1 as a key player in the progression of ESCC. The downregulation of EFNA1 inhibited cell proliferation and migration, activated cell cycle and autophagy. Additionally, a potential small molecule drug targeting EFNA1, salvianolic acid A (SAA), was found to suppress ESCC progression and enhance sensitivity to radiotherapy.
Article
Biochemistry & Molecular Biology
XueMei Li, Xin Zeng, DoYeon Kim, Jian Jiang, Fen Wei, JingYu Zhang, Bao Chai, Li Fu, Young Lee, ChangDeok Kim, HongXiang Chen
Summary: This study investigated the role of KLF4 in lipid production in immortalized human sebocytes during calcium-induced lipogenesis. The results showed that overexpression of KLF4 increased lipid production and upregulated the expression of lipogenic genes, suggesting that KLF4 is a novel regulator of lipid production in sebocytes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Food Science & Technology
Ya-Hui Ge, Xue Li, Mingzheng Huang, Zhengxu Huang, Manman Wu, Baoqing Sun, Lishuang Wang, Jian-Lin Wu, Na Li
Summary: We proposed an aroma correlation assisted volatilome coupled network analysis strategy to study Rosa roxburghii and discovered various aroma-active volatiles. The predominant aromas in R. roxburghii were found to be contributed by a series of homologous C6-and C8-acids, alcohols, aldehydes, esters, and terpenoids. Two aroma-active and aroma-contributing volatile groups, acid-aldehyde-alcohol-ester and terpenoid groups, were identified with the network analysis. Furthermore, the accumulation of C6-and C8-family homologous aliphatic volatiles was explained with linoleic and linolenic acid derived pathways. This strategy is practical for investigating the main aroma-active volatiles based on volatilome.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Xiang-Yu Tan, Yu-Ting Li, Hua-Hui Li, Li-Xiang Ma, Chui-Mian Zeng, Tian-Tian Zhang, Tu-Xiong Huang, Xiao-Di Zhao, Li Fu
Summary: Gastric cancer stem cells (GCSCs) exhibit chemoresistance due to the highly heterogeneous and immature phenotype. This study found that the WNT2-SOX4 positive feedback loop plays a crucial role in promoting chemoresistance and tumorigenicity in GCSCs. Blocking WNT2 disrupts this feedback loop and enhances chemotherapeutic efficacy in a mouse xenograft model.
Article
Cell Biology
Yu-Ting Li, Xiang-Yu Tan, Li-Xiang Ma, Hua-Hui Li, Shu-Hong Zhang, Chui-Mian Zeng, Liu-Na Huang, Ji-Xian Xiong, Li Fu
Summary: Gastric cancer is highly resistant to current therapies due to tumor heterogeneity, and cancer stem cells play a crucial role in this resistance. Overexpression of LGSN is correlated with malignant progression and poor survival in gastric cancer patients. Ectopic overexpression of LGSN in differentiated cells derived from gastric cancer stem cells facilitates dedifferentiation and treatment resistance.
CELL DEATH & DISEASE
(2023)
Review
Biochemistry & Molecular Biology
Na Xie, Guobo Shen, Wei Gao, Zhao Huang, Canhua Huang, Li Fu
Summary: Recent advances in neoantigen research have accelerated the development and regulatory approval of tumor immunotherapies, providing emerging targets for personalized cancer immunotherapies and potential predictors for tumor survival prognosis and immune checkpoint blockade responses.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)