Article
Chemistry, Analytical
Yong Wang, Dandan Wang, Guohua Qi, Ping Hu, Erkang Wang, Yongdong Jin
Summary: In this study, a gold nanoparticles nanomembrane-modified plasmonic glass nanopipette surface-enhanced Raman scattering (SERS) sensing platform was constructed to sensitively detect the content variation of intracellular miRNA-21. The proposed method provides a simple way to study intracellular substance change and evaluate cancer treatment outcomes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xiaowei Jiang, Yuan Zhou, Yuang Chen, Yuanhua Shao, Jiandong Feng
Summary: A low-voltage nanotweezer for trapping single DNA molecules has been developed by using etching-engineered nanoelectrodes, reducing the minimum trapping voltage and improving trapping stiffness. This advancement is attributed to the increase in spatial heterogeneity and nonuniformity of electric field near the nanoelectrodes caused by etching of quartz.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Hao Liu, Yusheng Fu, Rongzhi Yang, Jiuchuan Guo, Jinhong Guo
Summary: This paper provides a brief introduction to the development history and signal sensing principles of surface plasmon resonance (SPR) technology. It highlights the recent applications of SPR sensors in various fields, including environmental monitoring, food contamination detection, and medical diagnosis. The article also discusses personal perspectives and future development trends in sensor preparation and design, which are critical for improving the performance of SPR sensors.
Article
Optics
Li Long, Qiurong Deng, Rongtao Huang, Zhi-Yuan Li
Summary: A 3D printed spiral conical tapered gold tip was built on the end face of an optical fiber using two-photon polymerization direct laser writing technology and magnetron sputtering technique, serving as an efficient fluorescence enhancement device. The experimental results showed that the fluorescence enhancement factor of the gold tip bound to the fiber was about 38 times greater than the reference sample.
Article
Engineering, Biomedical
Congran Jin, Ziqian Wu, John H. Molinski, Junhu Zhou, Yundong Ren, John X. J. Zhang
Summary: Advancement in material science, particularly in the development of nanomaterials, has led to the creation of plasmonic biosensors that enable real-time diagnosis of various diseases through biomarker screening. These versatile and portable devices can be used in point-of-care settings, allowing for convenient and efficient testing of biomarkers in homes or elsewhere. This article reviews the recent progress in the design of these biosensors, including the development of nanomaterials and the applications in point-of-care diagnosis. Additionally, challenges and opportunities, such as incorporating machine learning algorithms and the testing of Covid-19, are discussed.
MATERIALS TODAY BIO
(2022)
Article
Chemistry, Analytical
Kunlun Jiang, Jingrui Wu, Yuwei Qiu, Yun Young Go, Kiwon Ban, Hyun June Park, Jung-Hoon Lee
Summary: The PPT-cPCR method is a fast, sensitive, inexpensive, and simple nucleic acid detection technique that allows for visual detection of DNA targets and easy access to quantitative information using a spectrophotometer and a smartphone.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Ding Jiang, Yude Zhang, Xiaojiao Du, Linhua Zhang, Xueling Shan, Wenchang Wang, Hiroshi Shiigi, Zhidong Chen
Summary: A novel near-infrared self-powered photoelectrochemical platform was constructed using nonmetallic plasmon Ti3C2 MXene and sulphur vacancy engineered Bi2S3. The continuous photoelectron injection from Bi2S3 to Ti3C2 MXene induced a stable SPR effect and high photoelectric conversion efficiency, enabling the development of high-performance NIR self-powered biosensors. As a proof of concept, a sensitive NIR self-powered sensor was constructed using Microcystin-RR as a model analyte.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Thakshila Liyanage, Meimei Lai, Gymama Slaughter
Summary: We have developed a novel label-free ultrasensitive tapered optical fiber plasmonic biosensor that can successfully detect a panel of microRNAs with good selectivity. The biosensor showed high refractive index sensitivity and achieved a limit of detection between 103 aM and 261 aM for the microRNA panel. It also demonstrated successful detection of clinically relevant concentrations of microRNAs in human serum with a LOD between 1.097 fM and 1.220 fM. This approach could potentially broaden the applications of biosensors for detecting and quantifying large arrays of microRNAs.
ANALYTICA CHIMICA ACTA
(2021)
Article
Physics, Applied
Chenwei Wei, Tun Cao
Summary: The proposed tunable plasmonic biosensor based on alpha-MoO3/graphene hybrid architectures can achieve high phase detection sensitivity, offering a new direction in the development of tunable ultrasensitive plasmonic biosensors.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Optics
Angel Ortega-Gomez, Mederic Loyez, Maxime Lobry, Karima Chah, Joseba Zubia, Joel Villatoro, Christophe Caucheteur
Summary: Bare and gold-coated tilted fiber Bragg gratings (TFBGs) are mature technologies for volume and surface refractometric sensing. Efforts are being made to produce more sensitive sensors for high-resolution screening of surroundings and detection of rare events. This study explores the development of TFBG refractometers in 4-core fibers, achieving high sensitivity and surface sensitivity with DNA probes immobilization as proof.
Article
Optics
Yi-Ru Li, Kuang-Li Lee, Kuan-Ming Chen, Yun Cheng Lu, Pin Chieh Wu, Sy-Hann Chen, Jiun-Haw Lee, Pei-Kuen Wei
Summary: This paper proposes a measurement method for sensitive and label-free detection of virus-like particles (VLPs) using color images of nanoplasmonic sensing chips. The experimental results show that the image contrast method has good linearity and can achieve direct detection of SARS-CoV-2 VLPs in a short period of time.
Review
Chemistry, Analytical
Gabriela Elizabeth Quintanilla-Villanueva, Jesus Maldonado, Donato Luna-Moreno, Jose Manuel Rodriguez-Delgado, Juan Francisco Villarreal-Chiu, Melissa Marlene Rodriguez-Delgado
Summary: Aquaculture is expanding to meet the increasing demand for high-quality protein, but it faces challenges such as pollution and lack of product quality assurance. Researchers are developing nanophotonic sensing technologies, like SPR and FO-SPR, to overcome these challenges and make aquaculture more sustainable and profitable. This review provides information on recent advances in these technologies and their applications in monitoring environmental hazards, fish health, disease, and biosecurity in aquaculture.
Article
Biotechnology & Applied Microbiology
Judith Camalo Vila, Nerea Castro-Aguirre, Gerardo A. Lopez-Munoz, Ainhoa Ferret-Minana, Francesco De Chiara, Javier Ramon-Azcon
Summary: Optical biosensors based on plasmonic nanomaterials have attracted significant scientific interest for their sensitivity, directness, and potential for multiplexing. A pilot study was conducted using optical discs as nanotemplates to develop an integrated plasmonic biosensor for monitoring cell adhesion and growth, showing promising results for real-time analysis of living cells behavior and culture conditions. This approach could offer a non-destructive and reliable tool for assessing cell viability and growth with low variability between technical replicates, improving experimental replicability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Dingdong Li, Rachel Odessey, Dongfang Li, Domenico Pacifici
Summary: The study introduces an effective surface immobilization protocol for capturing TREM2, a receptor associated with AD. Using this protocol, a biochemical sensing platform based on plasmonic interferometry was developed to detect physiological concentrations of TREM2 in solution. This approach could serve as an early indicator of AD dementia and be applied to develop other antibody tests.
Review
Chemistry, Analytical
Jeong Ah Park, Chaima Amri, Yein Kwon, Jin-Ho Lee, Taek Lee
Summary: Since 2010, DNA nanotechnology has made rapid advancements, providing new methods for constructing biosensors and overcoming the limitations of DNA solely as genetic material. The use of nucleic acids in biosensors has shown several advantages, and nanomaterials have also played a crucial role in advancing plasmonic biosensor technologies.
Article
Chemistry, Multidisciplinary
James Carthew, Hazem H. Abdelmaksoud, Karla J. Cowley, Margeaux Hodgson-Garms, Roey Elnathan, Joachim P. Spatz, Juergen Brugger, Helmut Thissen, Kaylene J. Simpson, Nicolas H. Voelcker, Jessica E. Frith, Victor J. Cadarso
Summary: The novel technology developed allows for direct imprint of micro- and nanoscaled topographical features onto the base of conventional cell cultureware, making it compatible with standard biological techniques and methods of analysis. High-throughput screening across five distinct cell types interrogated the effects of 12 surface topographies, demonstrating unique cell specific responses to behavior and cell morphological characteristics. This technology provides new insights into how surface topographies can regulate key image descriptors to drive cell fate determination in various fields in the life sciences.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Dimitris Missirlis, Miguel Banos, Felix Lussier, Joachim P. Spatz
Summary: In this study, a micropatterning strategy was proposed to introduce small molecules and ligands on patterns of arbitrary shapes on the surface of poly(acrylamide)-based hydrogels. The advantages of this approach include easy use, no need for prefabricated photomasks, the use of mild UV light and biocompatible bioconjugation chemistries, and the ability to pattern low-molecular-weight ligands. Cell patterning experiments demonstrated the response of cells to the combination of pattern shape and substrate elasticity.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Christoph A. Spiegel, Maximilian Hackner, Viktoria P. Bothe, Joachim P. Spatz, Eva Blasco
Summary: This paper presents a novel and versatile shape memory ink system that enables 4D printing at both the macroscale and microscale using light. The authors selected digital light processing (DLP) and direct laser writing (DLW) as suitable 3D printing technologies and developed a compatible ink system. They demonstrated the shape memory effect for various complex structures printed using DLP and showed remarkable shape memory properties for microstructures printed using DLW.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Multidisciplinary Sciences
Oskar Staufer, Kapil Gupta, Jochen Estebano Hernandez Buecher, Fabian Kohler, Christian Sigl, Gunjita Singh, Kate Vasileiou, Ana Yague Relimpio, Meline Macher, Sebastian Fabritz, Hendrik Dietz, Elisabetta Ada Cavalcanti Adam, Christiane Schaffitzel, Alessia Ruggieri, Ilia Platzman, Imre Berger, Joachim P. Spatz
Summary: This study investigates the mechanism of SARS-CoV-2 spike protein binding to fatty acids using synthetic minimal virions. The study reveals that the fatty acid binding pocket of the spike protein can act as an allosteric regulator, allowing the virus to adapt its immunogenicity in response to inflammation. The study also demonstrates that certain FDA-approved drugs can modulate cell binding of the spike protein in a similar manner to the fatty acid binding pocket. The findings provide insights into how SARS-CoV-2 dynamically interacts with the host immune response.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Felix Lussier, Martin Schroeter, Nicolas J. Diercks, Kevin Jahnke, Cornelia Weber, Christoph Frey, Ilia Platzman, Joachim P. Spatz
Summary: This study introduces a method that uses pH as a trigger to self-assemble droplet-stabilized GUVs, which improves the production efficiency of GUVs and allows for the generation of compartmentalized GUVs with flexibility and robustness.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Heyang Zhang, Jeroen Bussmann, Florian H. Huhnke, Joke Devoldere, An-Katrien Minnaert, Wim Jiskoot, Friedhelm Serwane, Joachim Spatz, Magnus Roding, Stefaan C. De Smedt, Kevin Braeckmans, Katrien Remaut
Summary: The study shows that packaging different mRNA encoding into the same lipoplexes is crucial for efficient delivery into individual cells. On the other hand, mixtures of lipoplexes containing only one type of mRNA tend to result in large variations in uptake and expression levels among individual cells.
Article
Chemistry, Multidisciplinary
Yixuan Jia, Christoph A. Spiegel, Alexander Welle, Stefan Heissler, Elaheh Sedghamiz, Modan Liu, Wolfgang Wenzel, Maximilian Hackner, Joachim P. Spatz, Manuel Tsotsalas, Eva Blasco
Summary: This article presents a method for manufacturing programmable materials using alkoxyamines for printing. By investigating different reaction processes, the mechanical properties of the material can be adjusted, and the size of the structure can be changed through polymerization reactions.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Virology
Fania Geiger, Tim Wendlandt, Tim Berking, Joachim P. Spatz, Christina Wege
Summary: A simple enzyme-mediated strategy enables site-specific covalent coupling of genetically tagged luciferase molecules to TMV-CoA, both in solution and on solid supports. The use of TMV-CoA for fishing ybbR-tagged proteins from complex mixtures could provide new opportunities for versatile equipment of miniaturized devices with biologically active proteins. The enzymes displayed on the protein coat of TMV nanocarriers exhibited high activity.
Article
Biochemical Research Methods
Andreas Fink, Charlotte R. Doll, Ana Yague Relimpio, Yannik Dreher, Joachim P. Spatz, Kerstin Goepfrich, Elisabetta Ada Cavalcanti-Adam
Summary: Spontaneous and induced front-rear polarization of cells is crucial for cell migration in various physiological and pathological conditions. The balance between adhesion and cytoskeleton-driven protrusion and retraction is important for adherent cell migration. A minimal model system using synthetic cells resembling giant unilamellar lipid vesicles (GUVs) was created to study the relationship between cytoskeleton organization and cell front-rear polarization. Micropatterned surfaces induced asymmetric deformation and alignment of actin filaments in adherent GUVs depending on their shape and size. This bottom-up approach lays the foundation for further understanding the mechanisms of cell migration.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Engineering, Biomedical
Sadaf Pashapour, Senne Seneca, Martin Schroeter, Friedrich Frischknecht, Ilia Platzman, Joachim Spatz
Summary: This study developed protein-based microcapsules derived from the extracellular matrix (ECM) to investigate the interactions between ECM components and pathogens and study disease infectivity. The microcapsules were created by using water-in-oil emulsion droplets as templates, allowing for the charge-mediated attraction of ECM proteins to the inner periphery of the droplets. The released ECM-based protein microcapsules containing E. coli showed differences in the behavior of E. coli depending on the surrounding ECM protein matrix.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Immunology
Sophie L. Preuss, Stephanie Oehrl, Hao Zhang, Thomas Doebel, Ulrike Engel, Jennifer L. Young, Joachim P. Spatz, Knut Schaekel
Summary: Formation and deposition of immune complexes (ICs) is a characteristic feature of autoimmune diseases. In this study, we demonstrated that ICs induce a specific migratory response, haptokinesis, in non-classical monocytes (ncMo) and in a specific subset of monocytes known as 6-sulfo LacNAc(+) monocytes (slanMo), but not in other monocyte subsets. CD16-dependent signaling and the activity of the metalloproteinase ADAM17 were found to mediate this migratory response.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Reinhard Lipowsky, Shreya Pramanik, Amelie S. Benk, Miroslaw Tarnawski, Joachim P. Spatz, Rumiana Dimova
Summary: Artificial or synthetic organelles are a challenge in bottom-up synthetic biology. Synthetic organelles are typically based on spherical membrane compartments for spatially confining chemical reactions. However, cellular organelles, like the endoplasmic reticulum (ER), have more complex architectures. The morphology of ER, including its straight appearance and nanoscopic shapes, as well as the formation and maintenance of its reticular networks, are closely related to the dimerization of membrane proteins and the generation of membrane tension.
Article
Chemistry, Multidisciplinary
Ana Yagu''e Relimpio, Andreas Fink, Duc Thien Bui, Sebastian Fabritz, Martin Schro''ter, Alessia Ruggieri, Ilia Platzman, Joachim P. Spatz
Summary: This study uses bottom-up assembled synthetic SARS-CoV-2 viruses to investigate the binding properties of the Omicron and Alpha variants. The results reveal a significantly higher affinity of Omicron S towards the lipid membrane and ACE2 receptor.
Article
Chemistry, Physical
Shreya Pramanik, Jan Steinkuehler, Rumiana Dimova, Joachim Spatz, Reinhard Lipowsky
Summary: His-tagged molecules can be attached to lipid bilayers via anchor lipids for biofunctionalization. Two fluorescent His-tagged molecules, GFP and FITC, were studied in this research. The brightness of membrane-bound 6H-GFP was found to exceed that of membrane-bound 6H-FITC, contrary to their quantum yields in solution. The membrane fluorescence was also measured as a function of the molar concentration of fluorophores, leading to the determination of equilibrium dissociation constants and a strong pH-dependence of membrane fluorescence.
Article
Engineering, Biomedical
Lucia T. Benk, Amelie S. Benk, Rafael B. Lira, Elisabetta Ada Cavalcanti-Adam, Rumiana Dimova, Reinhard Lipowsky, Benjamin Geiger, Joachim P. Spatz
Summary: This study utilizes droplet-based microfluidics to generate cell-sized giant unilamellar vesicles (GUVs) with a defined molecular composition, and quantifies the adhesion of integrin alpha(IIb)beta(3)-containing protocells in relation to the number of integrin-talin head domain (THD) complexes. The study shows that THD induces integrin clustering in protocells adhering to fibrinogen, which is an essential step in synthetic cell design. These results pave the way for further investigations of protein-protein interactions and assembly mechanisms within complex synthetic cells.
ADVANCED NANOBIOMED RESEARCH
(2022)
