Article
Multidisciplinary Sciences
Jantima Upan, Napaporn Youngvises, Adisorn Tuantranont, Chanpen Karuwan, Philippe Banet, Pierre-Henri Aubert, Jaroon Jakmunee
Summary: A label-free electrochemical aptamer-based sensor was developed for AFP detection, utilizing a PtNPs/GO-COOH modified platform with an AFP aptamer as the recognition element. The sensor demonstrated good sensitivity, selectivity, and stability for sensitive detection of AFP in serum samples.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
S. Reza Mahmoodi, Pengfei Xie, Daniel P. Zachs, Erik J. Peterson, Rachel S. Graham, Claire R. W. Kaiser, Hubert H. Lim, Mark G. Allen, Mehdi Javanmard
Summary: A non-faradaic label-free cortisol sensing platform was developed using a nanowell array design, allowing for rapid and low volume sensing. The sensor, built on a glass substrate, showed a good response to cortisol concentrations and compared well with results from standard ELISA. Nanowell array sensors have potential to be a promising platform for point-of-care testing.
Article
Chemistry, Analytical
Yuxi Wei, Jian Zhang, Xiaolin Yang, Zimei Wang, Junxia Wang, Honglan Qi, Qiang Gao, Chengxiao Zhang
Summary: This study presents washing-free electrogenerated chemiluminescence (ECL) magnetic microbiosensors for multiple protein biomarkers based on target assistant proximity hybridization (TAPH). The ECL method was developed for the determination of alpha-fetoprotein (AFP) with a much lower detection limit (LOD) of 0.04 ng mL(-1). The proposed strategy of ECL TAPH on magnetic glassy carbon electrode (MGCE) showed potential for qualifying other multiple protein biomarkers in real clinical assays.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Qing Li, Jing-Tao Wu, Ying Liu, Xiao-Man Qi, Hong-Guang Jin, Chun Yang, Jun Liu, Guang-Li Li, Quan-Guo He
Summary: Black phosphorus has attracted significant attention due to its anisotropic electro-conductivity and tunable direct bandgap, making it suitable for various electrochemical sensing applications. Research efforts have explored synthesis methods, protection strategies, and sensing applications of BP and its derivatives, along with functionalization mechanisms, sensing strategies, and properties.
ANALYTICA CHIMICA ACTA
(2021)
Article
Electrochemistry
Kavita Jayakumar, Richard Bennett, Donal Leech
Summary: Enzymatic glucose electrodes based on mediated electron transfer, with the inclusion of carbon nanosupports, can enhance characteristics such as current density and stability. However, large quantities of nanomaterials are often needed for significant effects. Grafting the enzyme to the surface of the carbon nanotubes improves dispersibility and enzyme activity, leading to optimised component amounts for increased current density and stability. Operational stability remains a challenge, with proposed methods for improvement including overcoating with a protective layer and implementing intermittent sampling of glucose levels.
ELECTROCHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Ekaterina Makhneva, Dorota Sklenarova, Julian C. Brandmeier, Antonin Hlavacek, Hans H. Gorris, Petr Skladal, Zdenek Farka
Summary: This study compares the effect of different labels and solid supports on the performance of heterogeneous immunoassays, and finds that the use of upconversion nanoparticles (UCNPs) and magnetic microbeads (MBs) can significantly improve the sensitivity and working range of biomarker detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Patta Supraja, Suryasnata Tripathy, Siva Rama Krishna Vanjari, Ranjana Singh, Vikrant Singh, Shiv Govind Singh
Summary: This study presents a facile, sensitive, and label-free electrochemical biosensor for detecting AB42 peptides, using electrospun SnO2 nanofibers as the transducing material. The sensor platform exhibits high sensitivity and stability, making it a promising tool for early diagnosis of Alzheimer's Disease.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Yang Wang, Mei-Hang Li, Xiao-Hong Wen, Meng-Yang Liu, Yan-Wei Lu, Yang Gu, Guang Zeng, Xue-Feng Zhao, Bao-Hong Liu, Xin-Ming Ji, Hong-Liang Lu
Summary: A label-free electrochemiluminescent immunosensor was developed for detecting glutamic acid decarboxylase65 antibody (GADA) in autoimmune diabetes. The immunosensor showed excellent sensitivity, specificity, and stability, making it a potential tool for clinical GADA detection.
Article
Biochemistry & Molecular Biology
Liang Yan, Chaoyan Zhang, Fengna Xi
Summary: In this work, a portable and highly sensitive disposable electrochemical immunosensing platform was developed for the detection of prostate-specific antigen (PSA). The platform was based on a chitosan-graphene-modified indium tin oxide (ITO) electrode and showed high selectivity, reproducibility, and good storage stability.
Review
Chemistry, Analytical
Pandiyaraj Kanagavalli, Chrysanthus Andrew, Murugan Veerapandian, Mani Jayakumar
Summary: This review provides a comprehensive overview of scalable fabrication methods and applications of hybridized graphene nanostructures as label-free electrochemical biosensor platforms, with a focus on the use of electrodeposition and electroless deposition techniques, as well as surface functionalization strategies.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Mahmoud Amouzadeh Tabrizi, Pablo Acedo
Summary: An interdigitated electrochemical immunosensor for the determination of PCT has been developed. The sensor showed good selectivity and stability, with a detection range of 2.5-800 pg/mL and a limit of detection of 0.36 pg/mL.
Article
Chemistry, Multidisciplinary
Supakeit Chanarsa, Jaroon Jakmunee, Kontad Ounnunkad
Summary: A bi-functional material based on silver nanoparticles-reduced graphene oxide composite was successfully synthesized for the construction of a label-free electrochemical immunosensor. The material showed good conductivity/electroreactivity and could generate an analytical response for the detection of human immunoglobulin G. Detection of IgG in human serum using the immunosensor displayed satisfactory accuracy and the potential for further development in clinical diagnosis.
FRONTIERS IN CHEMISTRY
(2021)
Review
Chemistry, Physical
Kouta Takeda, Nobuhumi Nakamura
Summary: Pyrroloquinoline quinone-dependent and flavin adenine dinucleotide-dependent enzymes catalyze the oxidation of various compounds, but the active sites embedded in the molecule make direct bioelectrocatalysis difficult. Researchers have attempted to connect electron transfer domains through protein engineering to achieve direct electron transfer. Recent reports have also shown enzymes without electron transfer domains are able to undergo direct electron transfer to an electrode.
CURRENT OPINION IN ELECTROCHEMISTRY
(2021)
Article
Biology
Chris Furlan, Nipa Chongdar, Pooja Gupta, Wolfgang Lubitz, Hideaki Ogata, James N. Blaza, James A. Birrell
Summary: Electron bifurcation is a fundamental energy conservation mechanism in nature that allows endergonic reactions to be driven by exergonic ones. In this study, the structure of HydABC, an electron-bifurcating [FeFe] hydrogenase, was determined using electron cryo-microscopy. The structure revealed a heterododecamer with a central electron transfer pathway, providing insights into the mechanism of electron bifurcation in HydABC. Based on these findings, a possible mechanism of electron bifurcation in HydABC was proposed.
Article
Chemistry, Analytical
Andres F. Cruz-Pacheco, Jennifer Quinchia, Jahir Orozco
Summary: A label-free nanoimmunosensor based on p53/CeO2/PEDOT nanobiocomposite-decorated screen-printed gold electrodes has been developed for the electrochemical detection of anti-p53 autoantibodies. This sensor offers high specificity, selectivity, and long-term storage stability and has the potential to detect anti-p53 autoantibodies in serum samples.
Article
Medical Laboratory Technology
Yu Jeong Choi, Juhye Roh, Sinyoung Kim, Kyung-A Lee, Younhee Park
Summary: This study assessed the correlation between IL-6 measurements obtained from the AFIAS IL-6 assay and Elecsys IL-6 assay, showing excellent agreement and high correlation. The AFIAS IL-6 assay demonstrated good analytical performances and IL-6 levels were found to be more accurate in predicting patient prognosis compared to C-reactive protein.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Multidisciplinary Sciences
June Seok Heo, Sinyoung Kim
Summary: This study found that human adipose mesenchymal stem cell-derived exosomes have anti-inflammatory and pro-angiogenic effects. Exosomes inhibit inflammation by activating ROCK1 and PTEN expression, and promote angiogenesis by releasing miR-132 and miR-146a.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, General & Internal
Namsoo Kim, Sinyoung Kim, Jong Rak Choi, Younhee Park
Summary: This study shared the experience of HLA typing using next-generation sequencing (NGS) in patients with hematologic malignancies and evaluated its association with hematologic diseases. The data from 211 Korean patients with a hematologic disease were reviewed, and NGS was performed for 11 HLA loci. The study found that NGS-based HLA typing enables detailed analysis of the HLA system and identified a specific HLA gene associated with myelodysplastic syndrome.
Article
Pathology
Junhyup Song, Jun Yong Kim, Sinyoung Kim, Younhee Park
Summary: This study evaluated the clinical utility of EBV real-time PCR compared to EBER-ISH in lymphoma patients. The results showed that EBV real-time PCR identified more positive cases for all subtypes except ENKTCL. Patients with ENKTCL and PTCL who were EBV DNA-positive had poorer overall survival. In addition, EBV DNA positivity was associated with worse overall survival in EBER-positive DLBCL and ENKTL, as well as EBER-negative PTCL patients.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2022)
Article
Immunology
Ji-Man Kang, Juhan Lee, Kyu Ha Huh, Dong Jin Joo, Jae Geun Lee, Hye Rim Kim, Ha Yan Kim, Myeongjee Lee, Inkyung Jung, Min Young Kim, Sinyoung Kim, Younhee Park, Myoung Soo Kim
Summary: This study compared the immune response of COVID-19 vaccines in solid organ transplant recipients and found that the mixed vaccine showed higher immunogenicity compared to the adenoviral vector vaccine and similar immunogenicity to the mRNA vaccine. However, the initial immune response was poor in all vaccine groups, suggesting the need for booster vaccination in transplant recipients.
Article
Medical Laboratory Technology
Juhye Roh, Sinyoung Kim, June-Won Cheong, Su-Hee Jeon, Hyun-Kyung Kim, Moon Jung Kim, Hyun Ok Kim
Summary: Blood group antigen-expressing nucleated red blood cells were successfully generated from induced pluripotent stem cells (iPSCs) for diagnostic purposes. iPSCs from rare blood group donors could serve as an unlimited source for reagent production.
ANNALS OF LABORATORY MEDICINE
(2022)
Article
Medical Laboratory Technology
Younhee Park, Juhye Roh, Sinyoung Kim
Summary: This study evaluated the performance of cobas 6800 and Aptima assays for quantifying viral load of HIV-1, HBV, and HCV, and found that both assays showed good agreement and correlation in their results.
ANNALS OF LABORATORY MEDICINE
(2022)
Article
Hematology
Soon Sung Kwon, Sinyoung Kim, Hyun Ok Kim
Summary: This study retrospectively reviewed ten-year hemovigilance data from a hospital in Seoul, Korea, and found that hypotensive transfusion reaction (HyTR) is rare in this hospital, with the highest incidence in filtered random donor platelets. Most cases occurred within 15 minutes after the start of transfusion and the majority recovered within an hour without severe complications. No prominent risk factors for HyTR were identified in this cohort.
Article
Virology
Ji-Man Kang, Juhan Lee, Kyu Ha Huh, Dong Jin Joo, Jae Geun Lee, Ha Yan Kim, Myeongjee Lee, Inkyung Jung, Min Young Kim, Sinyoung Kim, Younhee Park, Myoung Soo Kim
Summary: Immunogenicity following third-dose mRNA vaccine administration was good in Korean solid organ transplant recipients, but the neutralization effectiveness against the Omicron variant was weak, suggesting the need for additional preventive measures.
JOURNAL OF CLINICAL VIROLOGY
(2023)
Article
Cell & Tissue Engineering
Youn Keong Cho, Hyun-Kyung Kim, Soon Sung Kwon, Su-Hee Jeon, June-Won Cheong, Ki Taek Nam, Han-Soo Kim, Sinyoung Kim, Hyun Ok Kim
Summary: To address the blood shortage issue, scientists have been exploring the use of human-induced pluripotent stem cells (hiPSCs) to generate red blood cells (RBCs) in vitro. In this study, hiPSCs were established from peripheral blood, cord blood, and bone marrow, and it was found that peripheral blood and cord blood were the most reliable sources for RBC production in vitro. These findings will aid in the selection of optimal hiPSC lines for clinical RBC production in the future.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Oncology
Yae Jee Baek, Youn-Jung Lee, So Ra Park, Kyoo Hyun Kim, Seung-Hoon Beom, Choong-kun Lee, Sang Joon Shin, Sun Young Rha, Sinyoung Kim, Kyoung Hwa Lee, Jung Ho Kim, Su Jin Jeong, Nam Su Ku, Jun Yong Choi, Joon-Sup Yeom, Minkyu Jung, Jin Young Ahn
Summary: This study aimed to assess the humoral response to COVID-19 vaccination in solid cancer patients and analyze factors associated with immunogenicity. The results showed that cancer patients had lower antibody levels after the first vaccination compared to healthcare workers, but after the third vaccination, their antibody levels were comparable. However, the neutralizing antibody response to the Omicron variant was not robust in cancer patients who received the adenovirus vector-based vaccine as the primary series, suggesting the need for additional interventions to protect them from COVID-19.
CANCER RESEARCH AND TREATMENT
(2023)
Article
Microbiology
Junhyup Song, Sinyoung Kim, Eunmin Kwak, Younhee Park
Summary: In this study, the measurement procedure of CMV load in breast milk using the Cobas CMV test on the Cobas 6,800 system was evaluated. The study found CMV positivity rates in breast milk and neonatal samples to be 57.8% and 5.2% respectively, with a CMV transmission rate of 7.7%. The simplified sample preparation method showed equivalent or improved CMV detection sensitivity compared to the conventional method. This approach provides a convenient and reliable method for monitoring breast milk CMV positivity, contributing to the reduction of vertical CMV transmission.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Smriti Sinha, Akashlina Basu, Jai Shukla, Shirin Dasgupta, Gorachand Dutta, Soumen Das
Summary: A simple, reagent-free, non-destructive, smartphone-integrated paper-based device was developed for measuring blood hematocrit (Hct) level. The device, calibrated using a custom-made Python algorithm, showed wide linear range, high accuracy, and suitability for quantitative estimation and quality assessment in different medical settings.
ANALYTICAL METHODS
(2023)
Review
Chemistry, Analytical
Nidhi P. Rao, C. M. C. Vaishnavi, Malavika S. Kumar, S. Vishnu, Bimalendu Mukherjee, N. Karthik, Gorachand Dutta, Avijit Kumar Das
Summary: This review discusses the recent advancements in chemosensors for the selective detection of essential amino acids. Different sensing techniques, such as reaction-based approach, DNA-based sensors, nanoparticle formation, coordination ligand binding, host-guest chemistry, fluorescence indicator displacement (FID) approach, electrochemical sensors, carbon dot-based sensors, MOF-based sensors and metal-based techniques, are described.
ANALYTICAL METHODS
(2023)
Article
Biophysics
Nidhi Pandey, Mukti Mandal, Dibyendu Samanta, Gayatri Mukherjee, Gorachand Dutta
Summary: In this study, an ultrasensitive electrochemical biosensor based on a nanobody was developed for the quantitative detection of soluble CTLA-4. The sensor exhibited high sensitivity and selectivity, and could detect sCTLA-4 in serum samples with attomole sensitivity. It has the potential to facilitate timely diagnosis of disease progression and assist in patient selection for immunotherapeutic interventions.
BIOSENSORS & BIOELECTRONICS
(2023)