Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 16, Pages 8441-8447Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac5020657
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Funding
- National Natural Science Foundation of China [31301690]
- Doctoral Program of Higher Education Research Fund [20130097120006]
- Youth Innovation Fund in Science & Technology of Nanjing Agricultural University [KJ2013008]
- National Institute of Environmental Health Sciences Superfund Research Program [P42 ES04699]
- National Institute of Occupational Health Sciences Western Center for Agricultural Health Research [U50 OH07550]
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Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL(-1). The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection. This method provides positive results in the visual color of violet, while a negative response results in a sky blue color; therefore, the iLAMP allows one to rapidly detect analytes in yes or no fashion. We validated the iLAMP by detecting parathion-methyl, parathion, and fenitrothion in Chinese cabbage, apple, and greengrocery, and the detection results were consistent with the enzyme-linked immunosorbent assay (ELISA). In conclusion, the iLAMP is a simple, rapid, sensitive, and economical method for detecting OP pesticide residues in agro-products with no instrumental requirement.
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