Article
Biotechnology & Applied Microbiology
Linlin Cao, Sumei Lu, Chengjie Guo, Wenqiang Chen, Yinan Gao, Diwen Ye, Zejun Guo, Wanshan Ma
Summary: Dynamic monitoring of tumor markers is essential for the diagnosis of malignant tumors, evaluation of therapeutic effects, and prognosis analysis of cancer patients. This study utilized immunosensor technology to detect the digestive tract tumor marker CA242. PdAgPt nanocomposites, with great biocompatibility, electrical conductivity, and catalytic properties, were synthesized and loaded onto MoS2 to create a new nanocomposite. Optimizing the experimental conditions, the label-free CA242 electrochemical immunosensor showed a wide detection range and low detection limit. Additionally, the CA242 immunosensor demonstrated excellent repeatability, selectivity, and stability, and successfully detected CA242 in human serum samples. Therefore, the label-free electrochemical immunosensor constructed in this study holds great potential for the detection of clinical biomarkers.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Yunli Wang, Yaxing Hong, Ming Wang, Yingchun Zhu
Summary: The Ab2-PDA-AF nanolabels prepared in this study can achieve dual signal amplification for detecting AFP, exhibiting high selectivity, low detection limit, as well as excellent reproducibility and stability.
ACS APPLIED NANO MATERIALS
(2022)
Article
Biophysics
Wenxi Lu, Xixiang Xie, Xianli Lan, Pan Wu, Hongmei Peng, Jian He, Liping Zhong, Xiyu Liu, Zhiming Deng, Zhenkai Tan, Aiqun Wu, Liang Shi, Yong Huang
Summary: An ultrasensitive electrochemical immunosensor was developed for the detection of Glypican-3 (GPC3), a liver cancer marker. This sensor utilized the signal amplification of electro-active ferrocene-tyramine (Fc-Tyr) molecule deposition, catalyzed by horseradish peroxidase (HRP). The sensor demonstrated high selectivity and sensitivity to GPC3, and was validated through simultaneous testing with ELISA in clinical liver cancer patients.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Qianying Qiu, Xiao Ni, Tianchen Liu, Zening Li, Xinyi An, Xiaojun Chen
Summary: A novel electrochemical aptasensor was developed for quantitative detection of beta-Lg in hypoallergenic formula milk. By utilizing a tri-functional hairpin, the sensor exhibited a wide linear range and low detection limit for beta-Lg, demonstrating high selectivity, reproducibility, and stability, making it a promising platform for beta-Lg detection in real food samples.
Article
Chemistry, Analytical
Wenjun Li, Siyu Chen, Yue Yang, Yiju Song, Chaoyun Ma, Xiuwen Qiao, Chenglin Hong
Summary: A GOx/HRP@ZIF-90 nanomaterial with enhanced enzyme tolerance to external environment was developed using bio-simulated mineralization. The electrochemical immunosensor based on this nanomaterial showed excellent analytical performance in detecting CA-125, with good selectivity and stability, as well as high accuracy and precision in analyzing human blood samples.
Article
Chemistry, Analytical
Haijun Wang, Yuhang Song, Yaqin Chai, Ruo Yuan
Summary: The study prepared a nanocomposite Cu2+-PEI-Pt/AuNCs with multipath signal catalytic amplification for a sensitive electrochemiluminescence (ECL) immunosensor. The Cu2+-PEI-Pt/AuNCs system exhibited enhanced ECL signal due to the addition of polyethyleneimine (PEI) and Pt/Au nanochains (Pt/AuNCs), which acted as a co-reactant and mimicking enzyme, respectively, to promote the production of oxygen and co-reactive intermediate groups. Furthermore, the presence of Cu2+ further accelerated the decomposition of H2O2, resulting in an improved ECL response. The fabricated ECL immunosensor using Cu2+-PEI-Pt/AuNCs showed ultra-sensitive detection performance for α-1-fetoprotein.
Article
Biochemistry & Molecular Biology
Yao Wang, Ping Wang, Zhe Zhao, Sujie Ye, Wenzhong Wang, Qing Liu, Yang Li, Daopeng Zhang, Yueyun Li
Summary: A label-free electrochemical immunosensor based on PtCoCu nanoparticles supported on N and B-doped reduced graphene oxide (PtCoCu PNPs/NB-rGO) was developed for detecting beta-Amyloid(1-42) oligomers (A beta). The platform achieved dual amplification of electrocatalytic signal and effective immobilization of antibodies. Under optimal conditions, the immunosensor showed a wide linear range (50.0 fg/mL to 100 ng/mL) and low detection limits (3.5 fg/mL). This study demonstrates the promising potential of the immunosensor in sensitive detection of AD biomarkers.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Je Hyun Bae, Hyeri Jeon, Hyun Ju Yang, Chung Mu Kang, Donghoon Han
Summary: This study presents a new strategy for fabricating a fully integrated electrochemical platform. By controlling the electrodeposition conditions, the stability and uniformity of the films can be optimized. The fabrication of a three-electrode system and a solid-state reference electrode opens up possibilities for an electroanalytical platform.
Article
Chemistry, Analytical
Qi Yan, Gaiyun Zhao, Biao Wang, Ninggui Wang, Bawurejiang Duolihong, Xiaohong Xia
Summary: An electrocatalytic signal labeled immunosensor was constructed for ultrasensitive detection of carcinoembryonic antigen (CEA). Core-shell Au@CeO2 modified GCE was used to capture primary antibody (Ab1) and Au@CoFe-(oxy)hydroxide combined with secondary antibody (Ab2) served as the signal tag and amplifier. The proposed immunosensor showed a good linear response and a low detection limit, providing a novel research approach for clinical tumor marker detection.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jing Lu, Lulu Hao, Fei Yang, Yanju Liu, Huaixia Yang, Shuxun Yan
Summary: A novel electrochemical immunosensor was developed for the sensitive detection of the important biomarker CYFRA 21-1 in non-small cell lung cancer. The sensor utilized a ring-opening polymerization (ROP) signal amplification strategy to achieve high sensitivity and selectivity, showing a bright correlation in clinical serum samples. This method has great potential for ultrasensitive quantitation of biological molecules due to its ease of operation, environmental friendliness, and low cost.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Yaoyao Meng, Yueyuan Li, Shanghua Liu, Shujun Wang, Hui Dong, Feng Jiang, Qing Liu, Yueyun Li, Qin Wei
Summary: A sandwich-type electrochemical immunosensor was designed using a highly efficient catalytic cycle amplification strategy of CuFe2O4-Pd for sensitive detection of cardiac troponin I. The CuFe2O4 with varied valence metal elements showed favorable catalytic performance through bidirectional cycling of Fe2+/Fe3+ and Cu+/Cu2+ redox pairs. Cu+ acted as an intermediate product of the catalytic reaction, promoting the regeneration of Fe2+ and ensuring continuous recycling of the double redox pairs, significantly amplifying the current signal response. Pd nanoparticles loaded on the surface of amino-functionalized CuFe2O4 served as electrochemical mediators to capture labeled antibodies (Ab(2)), and also as co-catalysts of CuFe2O4 to enhance catalytic efficiency, thereby improving the sensitivity of the electrochemical immunosensor.
Article
Chemistry, Analytical
Di Cheng, Zhenbo Zhou, Shasha Shang, Hesen Wang, Huaimin Guan, Huaixia Yang, Yanju Liu
Summary: This study developed an electrochemical immunosensor based on the ROP reaction for highly sensitive detection of cTnI, showing good linearity and low detection limit. The ROP reaction method offers easy access to raw materials and mild reaction conditions, with excellent selectivity and reliability in serum sample detection.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Jing-Xian Zhang, Chun-Lin Lv, Chang Tang, Lu-Yao Jiang, Ai-Jun Wang, Jiu-Ju Feng
Summary: In this study, a sandwich-type electrochemical immunosensor was constructed using PtCo highly branched nanocrystals as electrode substrates and dendritic mesoporous SiO2@AuPt nanoparticles as signal labels for ultrasensitive detection of SCCA. The sensor exhibited a broader linear range and lower detection limit, along with good reproducibility and recovery in diluted human serum samples.
Article
Chemistry, Analytical
Sierra Jackson, Suji Lee, Abraham K. Badu-Tawiah
Summary: Microfluidic paper-based analytical devices (μPADs) are emerging as a prominent platform for disease detection in developing countries. However, their detection limits are inadequate and require test results to be read within a specific time interval for accuracy. This paper presents an on-chip mass spectrometry (MS) detection strategy for immunoassays performed on paper substrates and demonstrates its feasibility in malaria antigen detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Qingyu Wang, Haobo Sun, Dongxiao Wen, Lei Wang, Lianzhi Li, Jinming Kong, Xueji Zhang
Summary: An electrochemical sensing platform based on ATRP signal amplification strategy has been developed for ultrasensitive determination of breast and prostate cancer marker miRNA-141, with a linear range from 10 pM to 10 aM and a detection limit of 3.23 aM, providing a new and promising path for ultrasensitive analysis of tumor-related miRNAs.
Article
Chemistry, Analytical
Kyoungsoo Kim, YeJi Shin, ChaeHyun Lee, Hyeri Jeon, Seog Joon Yoon, Donghoon Han
Summary: Organic-inorganic hybrid perovskites play an important role in solar driven chemistry, but their characteristics can be influenced by an electrical field. By studying the spectroelectrochemical properties of hybrid perovskite quantum dots, the electrochemical stability and degradation process can be understood, providing strategies to enhance their operational performance.
Article
Chemistry, Analytical
Seol Baek, Donghoon Han, Seung-Ryong Kwon, Vignesh Sundaresan, Paul W. Bohn
Summary: Understanding the functional states of individual redox enzymes is vital for life. The use of zero-mode waveguides allows for studying single-enzyme reactions and has potential applications in molecular diagnostics.
ANALYTICAL CHEMISTRY
(2022)
Article
Optics
Yeonsu Woo, YeJi Shin, Lee Jae Hong, Gyu Tae Kim, Jeong Woo Byeon, Da In Jeong, Jaeseok Choo, Donghoon Han, Seog Joon Yoon
Summary: This study investigated the behavior of X-site halide and A-site cation exchange processes in perovskite quantum dots. It was found that applied bias and current could enhance the migration rate of ions, facilitating the ion interdot exchange reaction dynamics in between the perovskite quantum dots.
JOURNAL OF LUMINESCENCE
(2022)
Article
Chemistry, Physical
Soo Jeong Lee, You Jeong Lee, Sumi Seo, Hyeri Jeon, Donghoon Han, Hyunsik Im, Nabeen K. Shrestha, Seog Joon Yoon
Summary: By utilizing specific ligands and conducting post-annealing treatment, highly stable CsPbBr3 PQDs wrapped in silica shells were achieved, exhibiting excellent performance in electron transfer and photochemical reactions.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Electrochemistry
Gyu Seop Hwang, Woojun Shin, Gyeonghye Yim, Jae Hyuk Choi, Young-Kwan Kim, Hongje Jang, Yang-Rae Kim
Summary: This study investigates the relationship between the morphology of silver-containing Rh nanoparticles (RhNPs) and their performance in the hydrogen evolution reaction (HER). The RhNPs synthesized via inverse directional galvanic replacement show long-term stability and exhibit better performance compared to Pt/C and other RhNPs.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Seongjun Hong, Joon Ho Yoon, Seunghyun Jeong, Yang-Rae Kim, In Tae Kim
Summary: Three thiazole derivatives containing thiophene and selenophene were synthesized and used as monomers for electropolymerization. The electropolymerization process was studied using CV and EQCM techniques. The pseudocapacitor performance of deposited polymer layers was analyzed through CV and galvanostatic charging-discharging experiments. The research also investigated the influence of different chalcogenophenes and CV scan rates on the electropolymerization rate. Spectroelectrochemistry experiments revealed the optical and electrochromic behavior of the deposited polymer layers. EQCM experiments provided insights into anion intercalation/deintercalation processes and molecular weight analysis of solvated anions.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Physics, Applied
Seol Baek, Allison R. Cutri, Donghoon Han, Seung-Ryong Kwon, Julius Reitemeier, Vignesh Sundaresan, Paul W. Bohn
Summary: Structurally regular nanopore arrays with independently controllable electrodes enable electrochemical and optical investigations at the single entity level. These arrays have high analytical sensitivity for chemical sensing applications and can be augmented with additional nanopore layers for stimulus-responsive transport control mechanisms. The transparent bottom metal layer allows for optical access to the nanopore, enabling spectroelectrochemical investigations at the single molecule level.
JOURNAL OF APPLIED PHYSICS
(2022)
Article
Electrochemistry
Jinju Kim, Jinsik Shin, Hyun Ju Yang, Chung Mu Kang, Donghoon Han, Je Hyun Bae
Summary: This study investigates the influence of ion size and hydrophobicity on the charging dynamics of nanoporous electrodes. As the size of ions decreases and their hydrophobicity increases, the capacitance increases and the movement of ions within the nanopores becomes less restricted. The microporous Pt electrode exhibits better performance than the mesoporous carbon electrode when used with a hydrophilic electrolyte.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Gyu Seop Hwang, Woojun Shin, Gyeonghye Yim, Jae Hyuk Choi, Young-Kwan Kim, Hongje Jang, Yang-Rae Kim
Summary: Rh nanoparticles with different morphologies were synthesized and their electrocatalytic activity in the oxygen reduction reaction (ORR) was investigated. The nanoshell-structured Rh nanoparticles exhibited superior ORR activity and stability, indicating the importance of controlling the morphology for efficient electrocatalysis.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Soo Jeong Lee, You Jeong Lee, Sumi Seo, Hyeonyeong Jo, Donghoon Han, Seog Joon Yoon
Summary: Organometallic lead halide perovskites have excellent photophysical properties and have been successfully applied to various applications. This study investigated the impact of surrounding solvents on the properties of dimensionally confined perovskite quantum dots (PQDs) and observed the photoinduced charge transfer from PQDs to transparent conducting oxides.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Hyein Lee, Kyoungsoo Kim, Chung Mu Kang, Aeri Choo, Donghoon Han, Joohoon Kim
Summary: In this study, we developed a FLIM-based strategy for spatially resolved imaging of local faradaic processes on nanoscale electrochemical interfaces. By using Amplex Red as a fluorogenic redox probe, we successfully visualized faradaic processes occurring on different-sized electrochemical interfaces.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Je Hyun Bae, Hyeri Jeon, Hyun Ju Yang, Chung Mu Kang, Donghoon Han
Summary: This study presents a new strategy for fabricating a fully integrated electrochemical platform. By controlling the electrodeposition conditions, the stability and uniformity of the films can be optimized. The fabrication of a three-electrode system and a solid-state reference electrode opens up possibilities for an electroanalytical platform.
Article
Electrochemistry
Sojin Park, Seongjun Hong, Suseong Kim, Jae Hyuk Choi, Su Jin Kang, Jeongyeon Choi, Yeonho Chung, Hakhyun Nam, Yang-Rae Kim
Summary: Two phenothiazine derivatives were synthesized as new redox mediators and compared with conventional mediators. Cyclic voltammetry experiments were performed to investigate the reaction mechanism, reversibility, and stability of the mediators. The number of electrons and protons involved in the redox processes was estimated, and thermodynamic parameters were evaluated based on the electrochemical behavior. The performance of the mediators in the oxidation of reduced nicotinamide adenine dinucleotide was also analyzed, confirming their similarity or superiority to other mediators.
ELECTROCHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Md Sharifuzzaman, Young Do Shin, Junghyun Yoo, Md Selim Reza, Yang-Rae Kim, Jae Y. Park
Summary: Despite advances in diabetes management, continuous glucose monitoring devices (CGMDs) still have disadvantages due to accuracy, low interference effect, precision, and stability. A new oxygen-insensitive polymeric glucose microneedle (MN) was developed, functionalized with an electron-transfer mediator and graphene oxide to enhance conductivity and sensor performance. The MN showed a wide linear range, low detection limit, high sensitivity, stability, selectivity, and fast response time. In vivo testing on rabbits demonstrated strong correlation between MN-measured interstitial fluid (ISF) glucose concentrations and blood glucose concentrations measured with a commercial glucometer.
Article
Chemistry, Physical
Hong Chul Lim, Yujin Cho, Donghoon Han, Tae Hyun Kim
Summary: This study introduces a novel electrocatalyst composed of Prussian blue (PB)-graphene quantum dot (GQD), which exhibits exceptional activity and stability. The symbiotic relationship between PB nanoparticles (NPs) and GQD accelerates electron transport, increases electrochemically active sites, and shortens mass transfer lengths. The presence of GQD mitigates the decomposition of PB NPs and ensures continuous electrocatalytic performance.
APPLIED SURFACE SCIENCE
(2024)