Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Article
Immunology
John A. Fissel, Carrie K. Holdren-Serrell, Warda Memon, Sean X. Zhang
Summary: One of the challenges in fungal infection treatment is the long time required for species identification. MALDI-TOF MS has shown potential in rapidly identifying bacteria and yeasts, and this study explores its use for identifying filamentous molds. Most isolates in the study could be identified by MALDI-TOF MS within three days.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
R. Gathercole, E. Tranfield, D. Xia, G. Perez-Cordon, G. Robinson, D. Timofte, F. Zendri, R. M. Chalmers
Summary: The study developed a method to purify Cryptosporidium spp. oocysts from clinical stool samples and evaluated them using mass spectrometry. The method produced distinct spectra specific to different Cryptosporidium species, demonstrating the utility of the purification method.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Chadaporn Chaiden, Janthima Jaresitthikunchai, Anusak Kerdsin, Nattakan Meekhanon, Sittiruk Roytrakul, Suphachai Nuanualsuwan
Summary: In this study, MALDI-TOF-MS analysis of extracted peptides was developed to improve the classification of S. suis serotypes. The method successfully differentiated highly pathogenic SS2 and SS14 from other serotypes, indicating its potential as an alternative approach for serotyping clinically pathogenic S. suis isolates.
Review
Microbiology
Xin-Fei Chen, Xin Hou, Meng Xiao, Li Zhang, Jing-Wei Cheng, Meng-Lan Zhou, Jing-Jing Huang, Jing-Jia Zhang, Ying-Chun Xu, Po-Ren Hsueh
Summary: MALDI-TOF MS has been used in clinical microbiology since 2010, offering advantages such as convenience, speed, accuracy, and low cost. It has shown promising results in rapid detection of highly virulent and drug-resistant pathogens, making it a valuable tool in clinical microbial identification.
Article
Infectious Diseases
Jichan Shi, Gexin Gao, Jing Pan, Lian-Peng Wu, Hongye Ning, Zhengxin Wu, Xinchun Ye, Xiangao Jiang
Summary: This study evaluated the clinical value of MALDI-TOF MS in detecting NTM by comparing the results with liquid culture and gene chip. The findings showed that MALDI-TOF MS had a high consistency with both methods and could be a rapid and effective method for identifying NTM strains and drug resistance.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Parasitology
Jurena Christiane Ebersbach, Marcello Otake Sato, Matheus Pereira de Araujo, Megumi Sato, Soeren L. Becker, Issa Sy
Summary: Schistosomiasis is a major tropical disease affecting millions of people worldwide. The current diagnostic methods have limitations, and this study developed and validated a species-specific database for adult Schistosoma identification. The study also evaluated the effects of different storage solutions on spectra profiles. The results showed that MALDI-TOF MS can reliably distinguish between S. mansoni and S. japonicum.
PARASITES & VECTORS
(2023)
Article
Plant Sciences
Ye Rin Jin, Myung Jin Oh, Heung Joo Yuk, Hyun Joo An, Dong Seon Kim
Summary: This study established a new mass spectrometry-based analysis procedure for red ginseng polysaccharides (RGPs), successfully qualitatively analyzing neutral and acidic RGPs, and identifying novel components. The new procedure shows great potential as a specific tool for searching and determining various polysaccharides in herbal medicines.
JOURNAL OF GINSENG RESEARCH
(2021)
Article
Plant Sciences
Yukun Liu, Xiaofei Nie, Jilong Wang, Zhenqi Zhao, Zhimei Wang, Fang Ju
Summary: Flavonoids in litchi seeds have broad-spectrum antiviral and antitumor activities, especially against breast and liver cancers. MALDI-MSI was used to detect and image the distribution of flavonoids in litchi seed tissue sections for the first time, revealing their heterogeneous distribution and potential for extraction.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemical Research Methods
Hui Li, Chunxia Ma, Meng Gao, Yong Li, Lei Xie, Dantong Zhao, Runhao Zhang, Guanhua Zhang, Wenhan Li, Rong Rong, Biao Kong
Summary: Two-dimensional material Ti3C2(OH)(x) shows potential for overcoming the limitations of MALDI-TOF-MS in analyzing small-molecular compounds. It offers advantages such as minimum background interference, high peak intensity, high salt and protein tolerance, good repeatability, and stability after storage.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Chi-Sheng Chen, Wen-Chi Chen, Sarah Y. Chang
Summary: A new mode of electromembrane extraction (EME) has been developed for detection via matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS). The method successfully extracted and analyzed posaconazole, and was applied to human serum samples.
Article
Food Science & Technology
Qi Wang, Xiaohui Li, Hongping Wang, Simeng Li, Chen Zhang, Xueying Chen, Jing Dong, Hua Shao, Jing Wang, Fen Jin
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) method was developed to obtain dynamic images of forchlorfenuron in oriental melons. The majority of forchlorfenuron was found in the exocarp and mesocarp regions of the melons and decreased within two days after application. The degradation rate obtained by MALDI-MSI was reliable and comparable to HPLC-MS/MS, indicating its practicability for pesticide degradation study.
Article
Materials Science, Multidisciplinary
Jan Michalek, Karel Stepka, Michal Kozubek, Jarmila Navratilova, Barbora Pavlatovska, Marketa Machalkova, Jan Preisler, Adam Pruska
MICROSCOPY AND MICROANALYSIS
(2019)
Article
Chemistry, Analytical
Antonin Bednarik, Jan Preisler, Dominika Bezdekova, Marketa Machalkova, Michal Hendrych, Jarmila Navratilova, Lucia Knopfova, Eugene Moskovets, Jens Soltwisch, Klaus Dreisewerd
ANALYTICAL CHEMISTRY
(2020)
Article
Oncology
Barbora Pavlatovska, Marketa Machalkova, Petra Brisudova, Adam Pruska, Karel Stepka, Jan Michalek, Tereza Necasova, Petr Benes, Jan Smarda, Jan Preisler, Michal Kozubek, Jarmila Navratilova
FRONTIERS IN ONCOLOGY
(2020)
Article
Chemistry, Analytical
Antonin Bednarik, Vadym Prysiazhnyi, Jan Preisler
Summary: A novel approach for VOCs analysis based on Au+ ion chemical ionization has been proposed, utilizing a commercially available mass spectrometer with ultrahigh resolving power and sub-ppm mass accuracy. The technique demonstrated excellent performance in analyzing various classes of organic molecules, expanding the available methods for VOCs analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
I Jennifer Gomez, Manuel Vazquez Sulleiro, Anna Doleckova, Nadezda Pizurova, Jirina Medalova, Antonin Bednarik, Jan Preisler, David Necas, Lenka Zajickova
Summary: GQDs synthesized using a fast microwave-assisted hydrothermal protocol exhibited emission from NUV-Vis to NIR range, with their structure and chemical composition elucidated using advanced NMR and spectroscopy techniques. The observed multicolor red-shifted fluorescence emission in cell imaging demonstrates the potential application of GQDs as bioimaging carriers, along with their non-cytotoxicity confirmed through ATP assay.
MATERIALS CHEMISTRY FRONTIERS
(2022)
Article
Chemistry, Analytical
Antonin Bednarik, Vadym Prysiazhnyi, Dominika Bezdekova, Jens Soltwisch, Klaus Dreisewerd, Jan Preisler
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Monika Koktava, Jan Valasek, Dominika Bezdekova, Vadym Prysiazhnyi, Barbora Adamova, Petr Benes, Jarmila Navratilova, Michal Hendrych, Petr Vlcek, Jan Preisler, Antonin Bednarik
Summary: In this study, a novel combination of metal oxide laser ionization mass spectrometry imaging (MOLI MSI) technique with off-line lipid derivatization by ozone was presented for the detection of fatty acids (FA) and their carbon-carbon double bond (C=C) positional isomers in biological tissues. The developed technique allows for the visualization of FA distribution and provides additional information about positional isomers.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Pavel Pleskunov, Vadym Prysiazhnyi, Daniil Nikitin, Tereza Kosutova, Miroslav Cieslar, Ivan Gordeev, Zdenek Krtous, Suren Ali-Ogly, Jan Somvarsky, Mariia Protsak, Kateryna Biliak, Ksenia Kishenina, Antonin Bednarik, Milan Dopita, Jan Preisler, Andrei Choukourov
Summary: Precise molecular identification of specific biomarkers in biological tissues is crucial for accurate diagnostics. In this study, we introduce a new method using monoisotopic niobium nanoparticles as imaging matrices, synthesized without wet chemistry. The niobium nanoparticles form an effective matrix for high-resolution imaging of complex biological samples.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Marek Stiborek, Lenka Jindrichova, Stanislava Meliorisova, Antonin Bednarik, Vadym Prysiazhnyi, Jiri Kroupa, Pavel Houska, Barbora Adamova, Jarmila Navratilova, Viktor Kanicky, Jan Preisler
Summary: This study presents a new technique for digital mapping of biomarkers in tissues using infrared laser ablation and counting intact gold nanoparticle tags. The technique demonstrated successful mapping of a proliferation marker in three-dimensional aggregates of colorectal carcinoma cells, generating sharp distribution maps of the specific biomarker. The developed methodology shows high accuracy and potential applications with multielemental mass spectrometers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
I. Jennifer Gomez, Manuel Vazquez Sulleiro, Nadezda Pizurova, Antonin Bednarik, Petr Lepcio, David Holec, Jan Preisler, Lenka Zajickova
Summary: Carbon dots (CDs) exhibit similar photophysical features despite different synthesis protocols. Understanding the structure is crucial for achieving desired properties. This study provides insight into the structure and formation of CDs, identifying the presence of molecular fluorophore species. Density functional theory calculations support the spontaneous formation of a compact network of molecular species. Additionally, the study comprehensively investigates the photoluminescence properties, paving the way for CD-based logic gate fluorescent sensors.
APPLIED SURFACE SCIENCE
(2023)
Article
Biochemical Research Methods
Michal Zalud, Vadym Prysiazhnyi, Antonin Bednarik, Jan Preisler
Summary: In this study, the detection of single silver nanoparticles was achieved using subatmospheric pressure laser desorption/ionization mass spectrometry (LDI MS). The authors discussed the sample preparation, measurement conditions, generated ions, and limiting experimental factors. The results showed that 84 to 95% of the deposited 80 nm silver nanoparticles were detected. The LDI MS platform presented in this study provides an alternative method for imaging the distribution of individual nanoparticles and has the potential for multiplexed mapping of low-abundance biomarkers in tissues.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
