Review
Biochemical Research Methods
Qiuqin Zhou, Annabelle Fueloep, Carsten Hopf
Summary: MALDI-MSI is a rapidly growing technique for visualizing the distribution of biomolecules in tissue sections, with challenges such as sensitivity and specificity. Recent developments in small organic MALDI matrices and on-tissue chemical derivatization reagents play a crucial role in improving image quality and expanding application areas.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Kilian Horatz, Marco Giampa, Zhi Qiao, Siver A. Moestue, Franziska Lissel
Summary: MALDI MS and MSI are powerful analytical methods in medical and pathological research. Polymerizing SOMs into P(SOMs) mitigates background interferences, enhances vacuum stability, while maintaining the advantages of the original matrices.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Ewelina Maslak, Wioletta Mista, Michal Zloch, Dominika Blonska, Pawel Pomastowski, Fernanda Monedeiro, Boguslaw Buszewski, Jolanta Mrochem-Kwarciak, Katarzyna Bojarska, Dorota Gabrys
Summary: This study used mass spectrometry identification method (MALDI) to analyze urine samples from prostate cancer patients undergoing radiotherapy. The presence of microorganisms in urine and their changes in response to treatment were examined. The study found that radiotherapy depleted the local microbiome, but also led to an increase in bacteria one month after treatment. The presence of leukocytes and proteins in urine correlated with a greater variety of bacteria in urine specimens.
Article
Chemistry, Analytical
Bo-Jhang Lin, Tien-Chueh Kuo, Hsin-Hsiang Chung, Ying-Chen Huang, Ming-Yang Wang, Cheng-Chih Hsu, Po-Yang Yao, Yufeng Jane Tseng
Summary: Mass spectrometry imaging (MSI) is a powerful tool for investigating the spatial distribution of molecules in samples. However, analyzing complex biological systems often requires multiple analytical techniques. Image registration is a critical step for combining MSI with other imaging techniques, but it can be challenging due to differences in spatial resolution and image characteristics. In this study, a web service called MSI Registrar (MSIr) was developed for automatic registration between MSI and histology, providing a more objective and efficient way to analyze molecular components in tissues.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Luminita-Ioana Cotirla, Abbas Kareem Wanas
Summary: In this paper, we introduce a certain family of normalized holomorphic functions defined in the open unit disk, and establish coefficient estimates for the first four determinants of the symmetric Toeplitz matrices for the functions belonging to this family. We also mention some known and new results that follow as special cases of our results.
Article
Biochemical Research Methods
Kasper Krijnen, Joel D. Keelor, Sebastian Boehm, Shane R. Ellis, Claus Koester, Jens Hoehndorf, Ron M. A. Heeren, Ian G. M. Anthony
Summary: Mass spectrometry imaging (MSI) is a surface analysis technique used for biological research, and multimodal imaging combining multiple imaging modes can provide a more comprehensive view of samples. In this study, a prototype Bruker timsTOF fleX instrument was modified to include secondary ion mass spectrometry (SIMS) and secondary electron (SE) imaging capabilities while preserving matrix-assisted laser desorption/ionization (MALDI) capability. The modified instrument showed improved efficiency in multimodal imaging, allowing for easy registration of images and eliminating the need for sample transfer.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Zufeng Qiu, Zhiping Zheng, Zhijia Song, Yunchao Sun, Qiheng Shan, Zhiwei Lin, Zhaoxiong Xie
Summary: In this research, tricobalt tetraoxide (Co3O4) nanocrystals with rich surface hydroxyl groups were synthesized as novel matrices for the detection of small molecules by MALDI-TOF MS. These nanocrystal matrices showed little interference and high signal intensity, making them suitable for the analysis of amino acids, harmful additives, and pesticide residues.
Article
Agricultural Engineering
Jiao Wu, Chuang Cui, Hongwei Zhao, Guangzhen Zhou, Liang Qin, Xinyu Li, Lulu Chen, Xiaodong Wang, Yinglang Wan
Summary: Arecoline, a prevalent alkaloid in areca fruits, is considered the fourth most consumed addictive drug globally. This study utilized multiple techniques to analyze the alkaloids in areca fruits and provided information on their distribution in different tissues, contributing to the optimal utilization of areca fruits.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Margaux Fresnais, Esra Yildirim, Seda Karabulut, Dirk Jaeger, Inka Zoernig, Julia Benzel, Kristian W. Pajtler, Stefan M. Pfister, Jurgen Burhenne, Walter E. Haefeli, Remi Longuespee
Summary: Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been underutilized in therapeutic drug monitoring due to the complexity of ionization processes. Successful development of MALDI-MS methods requires consideration of matrix-analyte compatibility, matrix crystallization properties, and MS instrumentation. Controlling key parameters, addressing unresolved issues, and considering future perspectives are essential for establishing MALDI-MS as a universal method for drug monitoring.
Review
Chemistry, Analytical
Roman S. Borisov, Mariya D. Matveeva, Vladimir G. Zaikin
Summary: In recent years, there has been a special focus on the use of reactive matrices in MALDI-MS, which have dual properties and have been applied in bioanalysis to reduce sample treatment time and improve sensitivity. True reactive matrices interact with molecules through exchange or addition reactions, enhancing molecular identification and profiling.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2021)
Article
Oncology
Magdalena Buszewska-Forajta, Pawel Pomastowski, Fernanda Monedeiro, Justyna Walczak-Skierska, Marcin Markuszewski, Marcin Matuszewski, Michal J. Markuszewski, Boguslaw Buszewski
Summary: Prostate cancer is a major cause of cancer death in men, often recognized late due to lack of fast diagnostic methods. This study used mass spectrometry to analyze lipid alterations in prostate tissue, aiming to develop a quick and sensitive diagnostic method and improve hormone therapies effectiveness. The results suggest that lipidomics could serve as an alternative diagnostic tool for prostate cancer diagnosis.
Article
Chemistry, Multidisciplinary
Qiuqin Zhou, Stefano Rizzo, Janina Oetjen, Annabelle Fueloep, Miriam Rittner, Hartmut Gillandt, Carsten Hopf
Summary: Insufficient vacuum stability of matrix chemicals is a major limitation in MALDI mass spectrometry imaging of large tissue sample cohorts. A photo-cleavable caged molecule, DMNB-2,5-DHAP, was designed and synthesized for lipid MALDI-MSI of mouse brain tissue sections. DMNB-2,5-DHAP exhibited vacuum stability in a high vacuum MALDI ion source and its uncaging process resulted in the generation of 2,5-DHAP with the assistance of the built-in laser in the MALDI ion source.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Biochemical Research Methods
Jessica L. Moore, Georgia Charkoftaki
Summary: This review discusses recent advances in imaging mass spectrometry, specifically matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) applied on tissues. It explores sample preparation strategies, considerations for different analyte classes, and the use of multimodal imaging strategies. The review also examines experimental design approaches, standardization of processes, and data analysis platforms. It concludes with applications of imaging mass spectrometry in various fields, with a focus on medical research.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Chenglong Sun, Chunxia Ma, Lili Li, Yuhao Han, Daijie Wang, Xiao Wan
Summary: The application of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to investigate the changes of lipids in biological tissues has yielded significant results. However, the presence of structural isomers with varying C=C double bond (DB) locations calls for the development of isomer-resolved MSI. In this study, a new light-driven on-tissue [2 + 2] cycloaddition reaction coupled with MALDI-MS/MS imaging was introduced to identify lipid DB position isomers and their spatial signatures in biological tissues.
CHINESE CHEMICAL LETTERS
(2022)
Article
Biochemical Research Methods
Tina B. Angerer, Jerome Bour, Jean-Luc Biagi, Eugene Moskovets, Gilles Frache
Summary: Mass spectrometry imaging is a technique used to localize and identify lipids in tissue samples. By utilizing an AP-MALDI source coupled to an Orbitrap Elite, lipid locations and structures can be determined with high mass resolution spectra and cellular spatial resolution. Different MALDI matrices and treatment protocols have varying effects on the lipid coverage and signal intensity in positive and negative ion modes.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Letter
Neurosciences
Ming Lin, Jiamin Xu, Zhimin Liu, Liang Qin, Xiaodong Wang, Xiaoping Pu
NEUROSCIENCE BULLETIN
(2020)
Article
Environmental Sciences
Yanliang Zhang, Liang Qin, Jingkuan Sun, Lulu Chen, Lizhi Jia, Jinqi Zhao, Hongjun Yang, Kun Xue, Xiaodong Wang, Weiguo Sang
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2020)
Article
Chemistry, Analytical
Jian Song, Shuyu Lai, Dingnan Wang, Xiaomei Wu, Yiming Zhang, Linjiang Pang, Tingting Chai, Ji Zhao, Xiaodong Wang
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Chemistry, Analytical
Haiqiang Liu, Manman Han, Jinming Li, Liang Qin, Lulu Chen, Qichen Hao, Dongxu Jiang, Difan Chen, Yuanyuan Ji, Hang Han, Chunlin Long, Yijun Zhou, Jinchao Feng, Xiaodong Wang
Summary: This study demonstrated that caffeic acid (CA) can effectively enhance the detection and imaging of high-molecular-weight (HMW) proteins in biological tissue sections through matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). Compared to sinapinic acid (SA) and ferulic acid (FA), CA showed superior performance in detecting HMW proteins in biological tissues. Additionally, the use of a CA matrix significantly improved the imaging of proteins on selected tissue sections, demonstrating its potential as a standard organic acid matrix for tissue imaging of HMW proteins.
ANALYTICAL CHEMISTRY
(2021)
Article
Oncology
Chunyan Lan, Hainan Li, Lei Wang, Jing Zhang, Xiaodong Wang, Rumeng Zhang, Xiaoai Yuan, Taihua Wu, Jie Wu, Ming Lu, Xu Ma
Summary: The method using MALDI MSI for absolute quantification of 2-HG is effective in identifying IDH mutations and evaluating tumor residue. It has 100% specificity and sensitivity in IDHmt gliomas, and shows a positive correlation between 2-HG levels and tumor cell density.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Plant Sciences
Jialin Du, Weiwei Ma, Yi Li, Xu Lu, Zhaopeng Geng, Hangjun Huang, Yuanyuan Yuan, Yue Liu, Xiaodong Wang, Junli Wang
Summary: In this study, a non-targeted metabolomics approach was used to evaluate the effects of plant growth regulators (PGRs) 6-BA and KT on Scabiosa tschiliensis, showing differential impacts on metabolism and increased production of secondary metabolites. Primary metabolites such as amino acids were significantly reduced under PGR treatment, potentially transformed into secondary metabolites with biological activity that promote plant growth.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Yiyang Shi, Hao Hu, Qichen Hao, Ran Wu, Lei Wang, Liang Qin, Wei Gu, Haiqiang Liu, Dongxu Jiang, Liya Hong, Yijun Zhou, Xiangyi Liu, Jinchao Feng, Kun Xue, Xiaodong Wang
Summary: Michler's ethylketone (MEK) has been successfully optimized as a novel matrix for the enhancement of lipid detection and imaging in tissues by MALDI-MSI. Its properties as a powerful MALDI matrix include strong UV absorption, mu m-sized crystals, uniform matrix-coating, super high vacuum chemical stability, low matrix-related ion interference, super soft ionization, and high lipid ionization efficiency.
CHEMICAL COMMUNICATIONS
(2022)
Article
Plant Sciences
Xiaohong Qi, Lulu Chen, Zijian Hu, Weiwei Shen, Huimin Xu, Lingyu Ma, Guangchao Wang, Yanping Jing, Xiaodong Wang, Bolin Zhang, Jinxing Lin
Summary: This study investigated the morphological, cytological, transcriptional, and chemical changes during the late maturation stage of elm seeds, providing insights into the final stage of seed development in woody plants. The results revealed significant changes in cellular features and chemical composition, offering a valuable reference for understanding seed development in woody perennial plants.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Cell Biology
Wenqian Xie, Shengheng Mu, Jinkun Zhong, Chaoran Zhang, Haolin Zhang, Xiaodong Wang, Qiang Weng
Summary: In this study, the distribution and expression of lipids in the scent glands of muskrats during different reproductive statuses were investigated using imaging mass spectrometry and quantitative real-time PCR. The results showed significant differences in lipid expression and spatial distribution in the scent glands of muskrats during different reproductive statuses. Additionally, the expression levels of certain lipid molecules and lipid synthesis enzymes were higher during the breeding season. The study suggests that seasonal changes in lipid metabolism may affect the function of muskrat scent glands.
Article
Agricultural Engineering
Jiao Wu, Chuang Cui, Hongwei Zhao, Guangzhen Zhou, Liang Qin, Xinyu Li, Lulu Chen, Xiaodong Wang, Yinglang Wan
Summary: Arecoline, a prevalent alkaloid in areca fruits, is considered the fourth most consumed addictive drug globally. This study utilized multiple techniques to analyze the alkaloids in areca fruits and provided information on their distribution in different tissues, contributing to the optimal utilization of areca fruits.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Zhaopeng Geng, Na Hao, Jialin Du, Xu Lu, Yuanyuan Yuan, Yue Liu, Juanling Li, Minjuan Wang, Xiaodong Wang, Junli Wang
Summary: This study investigates the regulatory influences of 6-BA and KT in the rapid micropropagation system of A. hancockii. It is found that both 6-BA and KT can up-regulate the endogenous metabolite content and have different effects on aromatic components. Cytokinins significantly affect the aromatic components of A. hancockii, and plant tissue culture is an important means to alter the pattern of aromatic components in aromatic plants.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Chemistry, Analytical
Hualei Xu, Qichen Hao, Haiqiang Liu, Lulu Chen, Ran Wu, Liang Qin, Hua Guo, Jinrong Li, Chenyu Yang, Hao Hu, Kun Xue, Jinchao Feng, Yijun Zhou, Biao Liu, Gaopeng Li, Xiaodong Wang
Summary: A new method named concentration-descending washing strategy (CDWS) with methanol (MeOH) was successfully developed to enhance the detection and imaging of endogenous proteins in tissues using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). Compared to commonly used washing strategies, MeOH-CDWS significantly improved the number and intensity of protein ion signals and reduced protein delocalization, resulting in improved quality of protein images in tissue sections through MALDI-MSI.
Article
Agriculture, Multidisciplinary
Yue Yin, Zhentao Ren, Li Zhang, Liang Qin, Lulu Chen, Laipan Liu, Ruizong Jia, Kun Xue, Biao Liu, Xiaodong Wang
Summary: Transgenic soybean, with the largest cultivation area globally, poses ecological risks through gene flow to wild relatives. This study used MALDI-MSI and UPLC-Q-TOF-MS to analyze protein changes in transgenic soybean, wild soybean, and their F2 hybrid. Specific differentially expressed proteins (DEPs) in wild soybean were identified, while F2 seeds exhibited characteristics of both parents. Sucrose synthase and stress response-related DEPs were differentially expressed, potentially contributing to greater adaptability in offspring. MALDI-MSI showed potential as a visual method for transgenic soybean analysis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biology
Lulu Chen, Liang Qin, Yawen Zhang, Hualei Xu, Yufen Bu, Ran Wu, Haiqiang Liu, Qichen Hao, Hao Hu, Yijun Zhou, Jinchao Feng, Yanping Jing, Jun Han, Xiaodong Wang
Summary: This study investigates the germination mechanism of Chinese yew seeds, which are important for the production of the anticancer drug paclitaxel. The analysis of transcriptome, proteome, metabolome, and lipidome reveals the crucial role of abscisic acid and gibberellic acid 12 in determining seed dormancy or germination. The study also demonstrates the energy supply pathways during seed germination and the influence of plant hormones and phospholipids on embryo development.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ran Wu, Liang Qin, Lulu Chen, Rui Ma, Difan Chen, Haiqiang Liu, Hualei Xu, Hua Guo, Yijun Zhou, Xiaodong Wang
Summary: The study introduced a novel method using copper adhesive tape on a non-conductive glass slide for in situ detection and imaging of proteins in FFPE tissue sections, resulting in a higher number of protein ions compared to traditional methods. This new approach has the potential to advance protein MALDI-imaging on non-conductive glass slides.
CHEMICAL COMMUNICATIONS
(2021)