Article
Chemistry, Physical
N. G. Korobeishchikov, I. Nikolaev, V. V. Atuchin, I. P. Prosvirin, A. Tolstogouzov, V Pelenovich, D. J. Fu
Summary: The study shows that a combination of high- and low-energy treatment modes can provide an efficient etching rate comparable to that of a monoatomic ion beam, reducing surface roughness to the angstrom level. XPS measurements reveal the preferential sputtering of oxygen and boron, as well as an increase in the concentration of lithium cations in both modes. While chemical alterations in the treated materials cannot be fully prevented, the damages from a cluster bombardment are much less than that from a monoatomic ion beam.
SURFACES AND INTERFACES
(2021)
Article
Chemistry, Physical
R. Wirecka, D. Lachowicz, K. Berent, M. M. Marzec, A. Bernasik
Summary: This work thoroughly investigates the influence of X-ray irradiation and two types of surface sputtering methods. Monoatomic sputtering reduces iron oxide to metallic iron in a short time, while argon cluster sputtering minimizes structural modifications. The Fe 2p spectra fitting procedure for nanoparticles is established based on XPS characterization of Fe3O4 single crystal. The combination of X-rays and cluster sputtering alters the morphology and solidifies the surface of samples. The Spot-by-Spot Cluster Procedure is proposed to characterize the layer-by-layer structure of nanoparticles without altering it. The structure of zinc, manganese, and zinc-manganese ferrite nanoparticles is determined using this procedure. Depending on the dopant, its incorporation and the state of iron are changed.
SURFACES AND INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Ivan Nikolaev, Pavel Geydt, Nikolay G. Korobeishchikov, Aleksandr Kapishnikov, Vladimir A. Volodin, Ivan A. Azarov, Vladimir Strunin, Evgeny Y. Gerasimov
Summary: This paper investigates the influence of surface modification on the characteristics and properties of AlN thin films on Si and glass-ceramics substrates. It is found that low-energy cluster ions have a high efficiency of surface smoothing. The optical properties and thickness distribution of the functional layer of AlN are also investigated, and the direction of crystallite texturing is observed using cross-sectional SEM.
Article
Chemistry, Analytical
Dino Metarapi, Andreas Schweikert, Ana Jerse, Martin Schaier, Johannes T. van Elteren, Gunda Koellensperger, Sarah Theiner, Martin Sala
Summary: Laser ablation in combination with ICP-TOFMS allows non-targeted multi-element imaging and screening unexpected elements in a sample. However, quantification is limited due to the preparation of calibration standards. This study presents a workflow for semiquantitative analysis using gelatin micro droplet standards, which was evaluated on gelatin samples and real-world bioimaging samples.
ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Yasmine Sommerer, Olena Ohlei, Valerija Dobricic, Derek H. Oakley, Tanja Wesse, Sanaz Sedghpour Sabet, Ilja Demuth, Andre Franke, Bradley T. Hyman, Christina M. Lill, Lars Bertram
Summary: Epigenome-wide association studies (EWAS) have gained popularity in assessing the connection between DNA methylation (DNAm) and phenotypes related to brain measures, cognitive function, and neurodegenerative diseases. This study used peripheral tissues such as blood, buccal swabs, and saliva as surrogates for brain tissue, and identified significant correlations of DNAm variability between buccal and prefrontal cortex samples. The correlated CpG sites were mostly located in promoter regions and were influenced by genetic factors.
CLINICAL EPIGENETICS
(2022)
Review
Chemistry, Analytical
Huihui Liu, Yixin Pan, Caiqiao Xiong, Jing Han, Xiao Wang, Junyu Chen, Zongxiu Nie
Summary: This paper provides an overview of the importance of endogenous small molecules in biological processes and their relevance to various diseases. It highlights the use of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) as a powerful tool for analyzing small molecules in situ. The paper discusses the workflow, applications, challenges, and future directions of MALDI-MSI for small molecule analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Cell Biology
Paola Perin, Riccardo Rossetti, Carolina Ricci, Daniele Cossellu, Simone Lazzarini, Philipp Bethge, Fabian F. Voigt, Fritjof Helmchen, Laura Batti, Ivana Gantar, Roberto Pizzala
Summary: The choroid plexus serves as a regulated gate between blood and cerebrospinal fluid in the brain. Despite its simple histology, this organ has remarkably complex functions, with local heterogeneities and interactions. Advanced techniques like iDISCO-based clearing and light-sheet microscopy help in understanding the intricate structure of the choroid plexus.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Physical
Myeongsu Na, Kitae Kim, Kyoungjoon Oh, Hyung Jin Choi, Chang Man Ha, Sunghoe Chang
Summary: Through the development of SCARF, a sodium cholate-based active delipidation method, it has been shown to clear and immunolabel thick tissues or whole organs more efficiently than methods based on SDS. SCARF also preserves endogenous fluorescence better and allows for faster and more efficient penetration of antibodies into tissues.
Article
Materials Science, Multidisciplinary
N. G. Korobeishchikov, I. V. Nikolaev, V. V. Atuchin, I. P. Prosvirin, A. V. Kapishnikov, A. Tolstogouzov, D. J. Fu
Summary: The damage caused by gas cluster ion bombardment on the surface of inorganic single crystals is still unresolved. In this study, the influence of the kinetic energy per atom in the clusters (E/N) on the physicochemical structure of KGd(WO4)2:Nd single crystals was evaluated using XPS, Raman spectroscopy, and XRD techniques. The results showed that high-energy mode with E/N of about 100 eV provides sufficient sputtering efficiency, while low-energy mode with a few eV energy causes minimal surface damage. The subsurface crystal structure remained undamaged after cluster bombardment, but an unexpected increase in relative concentration of O atoms by 12% and depletion of K atoms by a factor of 2 was detected in the subsurface layer of KGd(WO4)2:Nd crystals. The surface sputtering features of inorganic single crystals at different E/N modes are discussed.
MATERIALS RESEARCH BULLETIN
(2023)
Article
Chemistry, Applied
Sergio Passarella, Ettore Guerriero, Luisangela Quici, Giuseppe Ianiri, Marina Cerasa, Ivan Notardonato, Carmela Protano, Matteo Vitali, Mario Vincenzo Russo, Antonio De Cristofaro, Pasquale Avino
Summary: The quality of honey is evaluated based on certain commodity parameters, with a emphasis on the absence of Polycyclic Aromatic Hydrocarbons (PAHs). This study investigated the presence of 22 PAHs in 57 honey samples collected in Central Italy and identified the main contaminants and their sources. The results indicated that benzo[a]anthracene and phenanthrene were the main pollutants in the samples, suggesting constant exposure. Combustion was found to be the primary contamination source in Italian samples, while the addition of naphthalene during beekeeping practices played a significant role in other samples.
Article
Endocrinology & Metabolism
Wafaa Lounis, Khadidja Kessas, Zehor Chouari, Imene Benyettou, Abdenour Boumechhour, Faiza Chaib, Seghir Hadjadj Aoul, Gerard Lizard, Omar Kharoubi
Summary: This study examines the potential neuroprotective effects of flaxseed oil (FSO) on the brain activity of rat pups sired by females intoxicated with aluminum (Al). FSO, a rich source of omega-3 fatty acids, is found to have neuroprotective effects on the developing rat brains, correcting and ameliorating the abnormalities brought on by aluminum toxicity. The study includes neurobehavioral experiments, evaluation of prooxidant parameters and neurotransmitter activity, and lipid composition analysis of the brain membrane.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Physics, Applied
Jonas Drewes, Suren Ali-Ogly, Thomas Strunskus, Oleksandr Polonskyi, Hynek Biederman, Franz Faupel, Alexander Vahl
Summary: The study utilized in situ UV-Vis spectroscopy to investigate how the growth and transport of nanoparticles inside a gas aggregation source (GAS) were influenced by the Ar gas flow and operating pressure. It was found that the distribution of gas flow velocity strongly impacted the movement of nanoparticles and their positions inside the GAS depended on the gas flow. Computational fluid dynamic simulations were also conducted to identify areas where nanoparticles could be trapped or lost inside the GAS.
PLASMA PROCESSES AND POLYMERS
(2022)
Article
Biochemical Research Methods
Wen Xu, Zeyue Shen, Junliang Zhang, Tengyu Zhang, Huanming Wu, Chenlu Wang, Jiancheng Yu, Keqi Tang
Summary: The study demonstrates that hollow electrode capillary plasma ionization (HECPI) is a simple and effective ionization method with the potential to be an efficient tool for rapidly detecting gaseous samples online.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Article
Neurosciences
Ana R. Costa-Brito, Telma Quintela, Isabel Goncalves, Ana C. Duarte, Ana R. Costa, Fernando A. Arosa, Jose E. Cavaco, Manuel C. Lemos, Cecilia R. A. Santos
Summary: The research found that choroid plexus epithelial cells play an important role in the secretion and distribution of prolactin receptors, becoming an alternative source of prolactin in the brain.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Chemistry, Analytical
Qiong Liang, Siyu Liu, Wei Xu, Yanbing Zhai
Summary: In this study, a capillary-in-capillary electrospray ionization (CC-ESI) source was developed for spontaneous liquid sampling and quantitative analysis based on the capillary phenomenon and electrospray ionization mass spectrometry (ESI-MS). The CC-ESI had advantages of cheap price, high portability, disposability, quantitation capability, and adequate sensitivity.
ANALYTICAL CHEMISTRY
(2023)
Article
Archaeology
Elisabetta Neri, Caroline Bouvier, Laurence de Viguerie, Alain Brunelle, Nesrine Nasr, Fathi Bejaoui, Francois Baratte, Philippe Walter
Summary: This study analyzes the surface treatment of a Roman statue from the Roman theatre of Dougga in Tunisia using a multi-analytical protocol. The results confirm the use of ganosis on the statue and explore the application of ancient recipes for the first time, demonstrating the potential of Time-of-Flight Secondary Ion Mass Spectrometry analysis for studying ancient recipes.
JOURNAL OF CULTURAL HERITAGE
(2021)
Article
Biochemical Research Methods
Jean-Claude Tabet, Yves Gimbert, Annelaure Damont, David Touboul, Francois Fenaille, Amina S. Woods
Summary: This research investigated the production spectra of [M + Na](+) from diterpene diester species and low molecular mass metabolites using electrospray ionization (ESI). The formation of protonated salt structures was proposed to explain observed neutral losses of carboxylic acids, providing insight into sodium retention mechanisms. Furthermore, unexpected consecutive carboxylic acid losses under resonant excitation conditions were shown to have an exothermic character based on quantum calculations. The formation and function of protonated salt structures are expected to aid in better understanding and software-assisted interpretation of tandem mass spectra from small molecules, particularly in the field of metabolomics.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Thi Phuong Thuy Hoang, Nicolas Ritter, Jean-David Rodier, David Touboul
Summary: This study developed a rapid and efficient method for characterization and quantification of Polyglycerol esters of fatty acids (PGEs) using SFC-HRMS technology, including consideration of multiple key parameters for PGE production and analysis, as well as the establishment of structural characterization and quantification standard for PG3+1C18:1. The method was successfully applied to quantify PG3+1C18:1 in commercial products.
Article
Biochemical Research Methods
Caroline Bouvier, Sebastiaan Van Nuffel, Philippe Walter, Alain Brunelle
Summary: Time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging is a surface analysis technique that can identify and spatially resolve the chemical composition of a sample in a non-destructive manner, making it particularly suitable for analyzing painting samples in cultural heritage research.
JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Biochemistry & Molecular Biology
Jonathan Sorres, Teo Hebra, Nicolas Elie, Charlotte Leman-Loubiere, Tatyana Grayfer, Philippe Grellier, David Touboul, Didier Stien, Veronique Eparvier
Summary: This study discovered antiprotozoal activities of ovalicin derivatives produced by a fungus isolated from termites and revealed the related compounds and genes through metabolomic and genomic analyses.
Article
Chemistry, Multidisciplinary
Benjamin Zagiel, Taleen Peker, Rodrigue Marquant, Guillaume Cazals, Gabrielle Webb, Emeric Miclet, Claudia Bich, Emmanuelle Sachon, Roba Moumne
Summary: This study reports an efficient strategy for synthesizing large libraries of peptides with defined conformations. The strategy involves using dynamic combinatorial chemistry to graft amino acid side chains onto a well-ordered 3D peptide backbone. By combining rationally designed scaffolds with combinatorial side chain selection, this method provides an alternative approach for accessing peptide libraries with structures that cannot be genetically encoded, thus enabling the discovery of protein mimetics for poorly understood targets.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Biochemical Research Methods
Salome Poyer, Laurent Laboureur, Teo Hebra, Nicolas Elie, Guillaume Van der Rest, Jean-Yves Salpin, Pierre Champy, David Touboul
Summary: This study developed analytical strategies for the complete chemical characterization of annonaceous acetogenins, natural products that are responsible for atypical Parkinsonism. By using tandem mass spectrometry and copper adducts, the researchers were able to identify the position of ketones, oxygen rings, and hydroxyl substituents, providing valuable structural information.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Microbiology
Marceau Levasseur, Teo Hebra, Nicolas Elie, Vincent Guerineau, David Touboul, Veronique Eparvier
Summary: In this study, we proposed a workflow using a standardized protein and lipid extraction protocol and reliable spectral comparison algorithm in MetGem software for classification and identification of environmental microorganisms. Our method was validated and shown to be capable of classifying bacteria at the order and genus level, and fungi at the order level.
Article
Chemistry, Multidisciplinary
Arcadie Fuior, Diana Cebotari, Mohamed Haouas, Jerome Marrot, Guillermo Minguez Espallargas, Vincent Guerineau, David Touboul, Roman Rusnac, Aurelian Gulea, Sebastien Floquet
Summary: This paper investigates the synthesis, structural studies, and behavior in solution of coordination complexes built by the association of 14 representative thiosemicarbazone ligands with the cluster [Mo2O2S2]2+. The complexes are characterized in both solid state and solution using XRD and NMR techniques. The results demonstrate that the nature and position of substituents on the ligands strongly influence the coordination modes of the ligands as well as the numbers of isomers in solution.
Article
Chemistry, Analytical
Cyrille Santerre, Eldra Delannay, Pilar Franco, Nadine Vallet, David Touboul
Summary: This study compared the application of classical gas chromatography (GC) technique and supercritical fluid chromatography (SFC) technology for the analysis of lemon essential oil. The results showed that SFC-UV could be considered as an alternative to GC-FID for the characterization and quality control of lemon oil.
Article
Biochemistry & Molecular Biology
Manal Diab, Ana Mateo, Joumada El Cheikh, Zeinab El Hajj, Mohamed Haouas, Alireza Ranjbari, Vincent Guerineau, David Touboul, Nathalie Leclerc, Emmanuel Cadot, Daoud Naoufal, Carles Bo, Sebastien Floquet
Summary: Herein, the synthesis of a new class of compounds associating Keggin and Dawson-type Polyoxometalates (POMs) with a derivative of the anionic decahydro-closo-decaborate cluster [B10H10](2-) through aminopropylsilyl ligand (APTES) acting as both a linker and a spacer is reported. Three new adducts were isolated and characterized, including an unprecedented monofunctionalized SiW10 derivative stabilized through intramolecular H-H dihydrogen contacts. DFT and electrochemical studies revealed the electronic effect of grafting the decaborate cluster on the POM moiety and its impact on the hydrogen evolution reaction (HER) properties.
Article
Biochemical Research Methods
Aline Cournut, Ioana Silvia Hosu, Flavie Braud, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: This study developed a novel SALDI-MS approach based on steel plates, which were chemically modified and textured to achieve efficient peptide detection. Seven optimal steel plate surfaces were selected based on the criteria of D/I efficiency and deposit homogeneity, and compared with MALDI-MS analyses.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2023)
Article
Microbiology
Teo Hebra, Veronique Eparvier, David Touboul
Summary: A new two-step solid-state cultivation process was used to produce specific red azaphilones pigments, and their chemical diversity was explored. This method involved accumulating yellow and orange azaphilones from Penicillium sclerotiorum in the first step, and incorporating the desired functionalized nitrogen in the second step. The potential of this method was demonstrated by overproducing an azaphilone with a propargylamine side chain.
Article
Microbiology
Marion Schilling, Marceau Levasseur, Muriel Barbier, Lydie Oliveira-Correia, Celine Henry, David Touboul, Sibylle Farine, Christophe Bertsch, Eric Gelhaye
Summary: Fomitiporia mediterranea M. Fischer (Fmed) is a white-rot wood-decaying fungus associated with Esca disease in vineyards. This study aimed to decipher the specific mechanisms of Fmed in degrading grapevine wood structure and extractives.
Article
Microbiology
Iuliana Popa, David Touboul, Tilde Andersson, Eduardo Fuentes-Lemus, Cyrille Santerre, Michael J. Davies, Rolf Lood
Summary: Cutibacterium acnes, an abundant bacteria on the skin, relies on the secretion of its antioxidant protein RoxP to maintain skin health. The oxidation status of proteins and the composition of lipids in C. acnes are influenced by environmental oxidative conditions and the presence of the bacterial antioxidant system.
