Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 15, Pages 7033-7037Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac4010502
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Funding
- National Natural Science Foundation of China [21075118, 21205113]
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In the present work, the activities of protein kinase A (PKA) in cell lysates have been detected by a peptide microarray-based resonance light scattering assay with gold nanoparticle probes. Highly sensitive detection of PICA activity in 0.1 mu g total cell proteins of SHG-44 cell lysate (corresponding to 200 cells) is achieved by a selected peptide substrate. The experimental results also demonstrate that the assay can be employed to evaluate expression levels of PKA activity in different cell lines and chemical (e.g., Forskolin)-mediated PKA activity fluctuation in living cells. In addition, PKA inhibition by the inhibitor (H89) is shown, suggesting the potential for screening PKA inhibitors at the living cell level.
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