Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 15, Pages 7599-7605Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac401727d
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Funding
- NSF of China [20975104, 20935005, 21127901, 21210007, 91213305, 91132708]
- National Basic Research Program of China (973 programs) [2010CB33502, 2013CB933704]
- Chinese Academy of Sciences [KJCX2-YW-W25, Y2010015]
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This study demonstrates a microfluidic chip-based online electrochemical detecting system for in vivo continuous and simultaneous monitoring of ascorbate and Mg2+ in rat brain. In this system, a microfluidic chip is used as the detector for both species. To fabricate the detector, a single-channel microfluidic chip is developed into an electrochemical flow cell by incorporating the chip with an indium-tin oxide (ITO) electrode as working electrode, an Ag/AgCl wire as reference electrode, and a stainless steel tube as counter electrode. Selective detection of ascorbate and Mg2+ is achieved by drop-coating single-walled carbon nanotubes (SWNTs) and polymerizing toluidine blue O (polyTBO) film onto the ITO electrode, respectively. Moreover, the alignment of SWNT-modified and polyTBO-modified electrodes and the solution introduction pattern are carefully designed to avoid any cross talk between two electrodes. With the microfluidic chip-based electrochemical flow cell as the detector, an online electrochemical detecting system is successfully established by directly integrating the microfluidic chip-based electrochemical flow cell with in vivo microdialysis. The microfluidic system exhibits sensing properties with a linear relationship from 5 to 100 mu M for ascorbate and from 100 to 2000 mu M for Me. Moreover, this system demonstrates a high selectivity and stability and good reproducibility for simultaneous measurements of ascorbate and Mg2+ in a continuous-flow system. These excellent properties substantially render this system great potential for continuous and simultaneous online monitoring of ascorbate and Mg2+ in rat brain.
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