Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 17, Pages 8055-8060Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac4018988
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Funding
- NSF
- Burroughs Wellcome Fund Career Award
- James H. Ferry Fund for Innovation
- Joseph R. Mares endowed chair
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Many studies have demonstrated the concept of using free-radical polymerization reactions to provide signal amplification so that molecular recognition events indicative of disease states may be detected in a simple and low-cost manner. We provide the first systematic study of how the dissociation constant impacts detection sensitivity in these assays, having chosen a range of dissociation constants (nanomolar to picomolar) that is typical of those encountered in molecular diagnostic applications that detect protein protein binding events. In addition, we use experimental results to validate a mass-action kinetic model that may be used to predict assay performance as an alternative or supplement to the empirical approach to developing new polymerization-based amplification assays that has characterized the field to date.
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