Article
Biochemistry & Molecular Biology
Mantas Ziaunys, Vytautas Smirnovas
Summary: Aggregation of amyloidogenic proteins/peptides is associated with amyloid-related disorders, but effective treatments are limited. Recent studies have focused on naturally occurring molecules as potential anti-aggregation compounds, with promising results for EGCG. However, the stability of EGCG under physiological conditions is limited, and its autoxidation products may be the actual inhibitory compounds. This study investigates the association of different EGCG autoxidation products with non-aggregated insulin and their effects on aggregation and fibril structure, revealing a specific incubation time that affects the amyloid aggregation process.
Article
Nanoscience & Nanotechnology
Nicholas Andrikopoulos, Yuhuan Li, Aparna Nandakumar, John F. Quinn, Thomas P. Davis, Feng Ding, Nabanita Saikia, Pu Chun Ke
Summary: This study developed a biocompatible metal-phenolic network (MPN) consisting of epigallocatechin gallate (EGCG) and physiological Zn(II), which showed remarkable potency against amyloid aggregation in Alzheimer's disease. The MPN@AuNP nanoconstruct exhibited superior performance in inhibiting amyloid aggregation and toxicity compared to EGCG@AuNP, due to its larger surface area. The atomic coordination of Zn(II)-EGCG and the structure and dynamics of amyloid aggregation modulated by MPN were analyzed, suggesting potential applications for regulating AD pathology.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Multidisciplinary Sciences
Mantas Ziaunys, Kamile Mikalauskaite, Andrius Sakalauskas, Vytautas Smirnovas
Summary: The fluctuation in solution ionic strength has a significant impact on the inhibitory effect of the protein aggregation inhibitor, epigallocatechin-3-gallate, with different amyloid-forming proteins exhibiting unique responses.
Article
Engineering, Biomedical
Zejian Xu, Guancheng Shan, Nairong Hao, Lianwei Li, Tian Lan, Yabo Dong, Jiayu Wen, Ran Tian, Yan Zhang, Lianzhou Jiang, Xiaonan Sui
Summary: This study presents a strategy to tailor soy protein-derived amyloid fibrils (SAFs) into hydrogels using (-)-epigallocatechin-3-gallate (EGCG). EGCG can cleave SAFs and induce the formation of branched chains, leading to gel-like structures. SAFs-EGCG complexes show superior antibacterial activity and are non-toxic to human cells.
Article
Agricultural Engineering
Yu-qi Song, Guanglong Yao, Jian Chen, Na Li
Summary: This study explores the interaction between whey protein and epigallocatechin gallate (EGCG), and finds that the addition of β-cyclodextrin can regulate this interaction. The addition of EGCG reduces the light transmittance and fluorescence intensity of whey protein, while also weakening the antioxidant activity of EGCG. β-cyclodextrin improves certain properties of the EGCG-whey protein hybrid system.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Food Science & Technology
Baogui Liu, Zhiyin Chen, Yangling Zhang, Enshuo Liu, Shuming Han, Zhihua Gong, Wenjun Xiao
Summary: The study improved the bioavailability and efficacy of EGCG by preparing EGCG+LTA/βCD inclusion complexes, which significantly enhanced its lipid-lowering and weight loss effects by improving gut microflora and EGCG bioavailability.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemistry & Molecular Biology
Camilla B. Andersen, Yuichi Yoshimura, Janni Nielsen, Daniel E. Otzen, Frans A. A. Mulder
Summary: The study demonstrates that (-)-epigallocatechin gallate (EGCG) can reduce the toxicity of α-synuclein (α SN) oligomers by binding to both monomeric and oligomeric forms of α SN. EGCG binds to different regions of α SN with highest affinity for the monomeric form. Furthermore, kinetic data suggest a coupling between EGCG dimerization and α SN association.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Seungil Park, Gyeong Han Jeong, Sung Hoon Jee, Tae Hoon Kim, Seong Bong Kim
Summary: This study reported an efficient method for dimerization of EGCG, the major bioactive constituent isolated from tea leaves, using low-temperature plasma. The contribution of ozone in the reaction process was quantified as a major factor, and the mechanism of synthesizing dimers A and B through the generation of ozone and formaldehyde during plasma treatment was investigated.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Kumju Youn, Chi-Tang Ho, Mira Jun
Summary: Alzheimer's disease is characterized by cognitive decline and memory impairment, with amyloid peptide generation and aggregation playing a key role in its pathogenesis. (-)-Epigallocatechin-3-gallate (EGCG) has been shown to have multiple neuroprotective effects in AD, focusing on amyloid peptide-induced damage and regulation.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Engineering, Biomedical
Xiaoding Lin, Wei Liu, Xiaoyan Dong, Yan Sun
Summary: This study synthesized epigallocatechin gallate-derived carbonized polymer dots (E-CPDs) for the first time and demonstrated their multifunctional capability in inhibiting and disaggregating A,B fibrils, detecting amyloid plaques, and scavenging intracellular reactive oxygen species. The findings suggest that E-CPDs have great potential in the prevention and treatment of Alzheimer's disease (AD).
ACTA BIOMATERIALIA
(2023)
Review
Biochemistry & Molecular Biology
Anna Sulatskaya, Anastasiia O. Kosolapova, Alexander G. Bobylev, Mikhail Belousov, Kirill S. Antonets, Maksim Sulatsky, Irina M. Kuznetsova, Konstantin K. Turoverov, Olesya Stepanenko, Anton A. Nizhnikov
Summary: Both amyloids and beta-barrel proteins have beta-sheet-rich structures, with the latter being able to form functional amyloids in vivo. These beta-barrel amyloid proteins can interact with each other and form toxic oligomers, potentially contributing to the development of amyloidoses. Rapidly growing discoveries suggest that the number and diversity of functions of amyloid-forming beta-barrel proteins are significantly greater than currently understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Victor Valverde-Salazar, Daniel Ruiz-Gabarre, Vega Garcia-Escudero
Summary: Alzheimer's disease (AD) is a common form of dementia characterized by memory decline, cognitive impairment, and several pathological changes in the brain. Oxidative stress and inflammation play important roles in the development of AD. Green tea and its active compound EGCG have shown potential therapeutic effects in modulating AD through their antioxidative and anti-inflammatory properties. EGCG can regulate inflammatory processes, reduce protein aggregation, and promote neuronal survival pathways, making it a suitable candidate for the treatment of neurodegenerative disorders involving oxidative stress and inflammation.
Article
Chemistry, Physical
Chengyu Wang, Shuguo An, Zhongwei Li, Hao Chen, Zhenhu Yan, Yebang Tan
Summary: Natural polyphenols such as EGCG derived from green tea can be utilized to synthesize hyperbranched surfactants with excellent demulsification properties. These surfactants show high interfacial activity and efficient demulsification performance in aging oil emulsions from South China Sea Oilfield and Bohai Oilfield, achieving high dehydration ratios even at low concentrations and temperatures. The polyphenol structure and multiple branches of EGCG surfactants may contribute to their outstanding demulsification performance in various types of emulsions.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Agriculture, Multidisciplinary
Zonghan Wang, Fawen Yin, Deyang Li, Xinmiao Wang, Chao Guo, Donghong Liu, Beiwei Zhu, Dayong Zhou
Summary: This study proposed an ordered encapsulation strategy using cyclodextrin-based metal-organic frameworks (CD-MOFs) to alleviate the auto-browning phenomenon of catechins while preserving their antioxidant capability. The encapsulated catechins showed delayed browning and reduced generation of oxidative dimers and semiquinone radicals, even in aqueous solutions. The mechanism insights revealed that ordered encapsulation restricted molecular mobility and constrained molecular conformations of catechins.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Engineering, Chemical
Yue Liang, Wenjuan Wang, Yan Sun, Xiaoyan Dong
Summary: Inhibition of protein misfolding and aggregation is a major challenge in biochemical and biopharmaceutical engineering. This study investigated the coaggregation and seeding interactions between AB40 and AB11-40, as well as the effect of the small molecule inhibitor EGCG on amyloid aggregation. The results showed that AB11-40 participates in the aggregation of AB40 and that EGCG inhibits both AB isoforms mainly through hydrophobic interactions. These findings provide new insights into AB aggregation and have implications for the development of inhibitors against amyloid protein co-assembly.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
M. Nur Hossain, Syed Ahmad, Iranaldo Santos da Silva, Heinz-Bernhard Kraatz
Summary: The study demonstrated the synthesis of unique coinage metal nanodendrites as effective electrocatalysts for CO2 reduction, optimized their catalytic activity in an ethanolamine solution, and found formate as a product of the CO2 reduction process through spectroscopy analysis.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
M. Nur Hossain, Syed Ahmad, Heinz-Bernhard Kraatz
Summary: The consecutive incorporation of Ag(I) ions into dsDNA containing adjacent C-C mispairs was demonstrated, leading to structural changes and possible aggregation of DNA. Excess Ag-I immobilized DNA strands on Au surfaces, resulting in altered electrochemical properties.
Article
Chemistry, Inorganic & Nuclear
Junyan Wang, Mozhgan Khorasani Motlagh, Meissam Noroozifar, Kagan Kerman, Heinz-Bernhard Kraatz
Summary: In this proof-of-concept study, ferrocene-modified multiwalled carbon nanotubes were successfully synthesized and applied to a graphite paste electrode for the sensitive simultaneous determination of dopamine, uric acid, and xanthine. The modified electrode showed high stability, reproducibility, and repeatability, with a recovery rate of 95-104% for the simultaneous determination of the analytes in real samples.
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Tao Zhan, Xiao-Zhen Feng, Qi-Qi An, Shiyong Li, Mingyue Xue, Zhencheng Chen, Guo-Cheng Han, Heinz-Bernhard Kraatz
Summary: Gold electrodes were modified with copper nanoparticles and cobalt nanoparticles to prepare two enzyme-free electrochemical sensors. The ferrocene-peptide conjugate acted as an effective redox mediator for glucose oxidation. The sensors exhibited high sensitivity, 25 times higher than other glucose sensors, and showed good reproducibility and stability.
Article
Chemistry, Inorganic & Nuclear
Veronika Kovac, Ivan Kodrin, Kristina Radosevic, Kresimir Molcanov, Bimalendu Adhikari, Heinz-Bernhard Kraatz, Lidija Barisic
Summary: In this study, oxalamide derivatives of ferrocene peptides were synthesized and evaluated for their conformational properties, gelation ability, and cytotoxicity against different cell lines. The results showed that the oxalamide 3 had a higher degree of chiral organization and could self-assemble to form supramolecular nanostructures. Interestingly, the oxalamide 4 could form a stable gel when sonicated. Cytotoxic evaluation revealed that oxalamide 3 exhibited stronger growth-inhibitory effect on cancer cells compared to oxalamide 4 and cisplatin, indicating its potential as an anticancer agent.
Article
Chemistry, Multidisciplinary
Mozhgan Khorasani Motlagh, Meissam Noroozifar, Rana N. S. Sodhi, Heinz-Bernhard Kraatz
Summary: An enzymatic biosensor was developed for the determination of selenate using selenate reductase attached to a gold electrode. The modification of the electrode was characterized, and cyclic voltammetry and differential pulse voltammetry were used to measure selenate. The biosensor showed good performance in determining selenate in different water samples.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Electrochemistry
Tao Zhan, Xiao-Zhen Feng, Yun-Yun Cheng, Guo-Cheng Han, Zhencheng Chen, Heinz-Bernhard Kraatz
Summary: The study presents the construction of an electrochemical folic acid sensor using copper and ferrocene carboxylic acid (Fc(COOH)) on a glassy carbon electrode. The sensor exhibited good selectivity and stability, and showed satisfactory sensitivity and recovery rate in folic acid detection.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Electrochemistry
Qing-Min Lin, Xiao-Zhen Feng, Fang-Li Chen, Ke-Hang Song, Guo-Cheng Han, Zhencheng Chen, Heinz-Bernhard Kraatz
Summary: In this study, a novel electrochemical strategy for chloramphenicol (CAP) detection was proposed. The sensor, based on silver nanoparticles and thiophene modified glassy carbon electrode, demonstrated simple preparation, low cost, good repeatability, and anti-interference capability.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Mozhgan Khorasani Motlagh, Meissam Noroozifar, Heinz-Bernhard Kraatz
Summary: This study utilizes gold nanodendrites to immobilize selenate reductase on electrode surfaces for the selective catalytic reduction of selenate. The electrochemical sensor demonstrates a significant improvement in selenate detection compared to non-nanostructured surfaces.
CANADIAN JOURNAL OF CHEMISTRY
(2022)
Article
Electrochemistry
Chen-Wei Shan, Xiao-Zhen Feng, Guo-Cheng Han, Zhencheng Chen, Heinz-Bernhard Kraatz
Summary: We report an electrochemical sensor for the simultaneous detection of epinephrine (EP) and serotonin (5-HT), two important neurotransmitters in the mammalian central nervous system and serum. The sensor, based on cyclic voltammetry deposition of cobalt nanoparticles and melamine on a glassy carbon electrode, exhibited high sensitivity towards EP (0.23 V) and 5-HT (0.38 V vs. Ag/AgCl) detection. The linear range for EP and 5-HT was determined to be 5.00-500.00 mu mol center dot l(-1) with a limit of detection of 1.60 mu mol center dot l(-1) for EP and 2.52 mu mol center dot l(-1) for 5-HT.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Priyadarshani Choudhary, Heinz-Bernhard Kraatz, Celine M. Levesque, Siew-Ging Gong
Summary: This study aimed to develop a delivery system for LAB813, a newly identified S. salivarius strain. Polysaccharides such as alginate and chitosan were tested for encapsulating LAB813, and chitosan-coated alginate microcapsules showed superior antibacterial properties. These microcapsules retained their antibacterial activity for up to 28 days, highlighting their potential for improving oral health.
Article
Chemistry, Analytical
Yun-Yun Cheng, Xiao-Zhen Feng, Tao Zhan, Qi-Qi An, Guo-Cheng Han, Zhencheng Chen, Heinz-Bernhard Kraatz
Summary: Researchers developed a highly sensitive electrochemical immunosensor for C-reactive protein (CRP) detection using indole as an electrochemical probe and gold nanoparticles for signal amplification on a screen-printed carbon electrode. The sensor showed a logarithmic correlation between CRP concentration and response current, with a detection limit of 0.03 ng·mL-1 and a sensitivity of 5.7055 μ A·μ g-1·mL·cm-2. It exhibited excellent selectivity, reproducibility, and stability, making it promising for CRP detection in real human serum samples.
Article
Electrochemistry
Tsuimy Shao, Junyan Wang, Meissam Noroozifar, Heinz-Bernhard Kraatz
Summary: In this study, a ferrocene-peptide conjugate was synthesized and used for the detection of ascorbic acid. The Cu(II) complex of this conjugate showed high sensitivity and selectivity for ascorbic acid detection, and it was successfully applied to quantify ascorbic acid in commercial beverages.
Article
Chemistry, Analytical
Qi-Qi An, Xiao-Zhen Feng, Tao Zhan, Yun-Yun Cheng, Guo-Cheng Han, Zhencheng Chen, Heinz-Bernhard Kraatz
Summary: This study successfully synthesized a PPy-Au nanocomposite and demonstrated its potential for detecting hs-CRP in human serum samples.
Article
Materials Science, Multidisciplinary
Maryam Abdinejad, Mozhgan Khorasani Motlagh, Meissam Noroozifar, Heinz Bernhard Kraatz
Summary: By introducing bimetallic components, aerogel-supported Sn-Pd catalysts achieved selective electroreduction of CO2 to formic acid at ambient temperature, with further enhancement in electrocatalytic activity by introducing amino substituents.
MATERIALS ADVANCES
(2022)
