Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 19, Pages 9156-9163Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac4018154
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Funding
- Baden-Wurttemberg Ministry of Science and Culture [INST 874/2-1 LAGG]
- ZO IV by the Landesstiftung Baden-Wurttemberg
- Europaischer Fonds fur regionale Entwicklung (EFRE)
- Phospholipid-Forschungszentrum e.V., Heidelberg, Germany
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Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) has become a method of choice in lipid analysis, as it provides localization information for defined lipids that is not readily accessible with nonmass spectrometric methods. Most current MALDI matrices have been found empirically. Nevertheless, preferential matrix properties for many analyte classes are poorly understood and may differ between lipid classes. We used rational matrix design and semiautomated screening for the discovery of new matrices suitable for MALDI-IMS of lipids. Utilizing Smartbeam- and nitrogen lasers for MALDI, we systematically compared doubly substituted alpha-cyanocinnamic acid derivatives (R-1-CCA-R-2) with respect to their ability to serve as negative ion matrix for various brain lipids. We identified 4-phenyl-alpha-cyanocinnamic acid amide (Ph-CCA-NH2) as a novel negative ion matrix that enables analysis and imaging of various lipid classes by MALDI-MS. We demonstrate that Ph-CCA-NH2 displays superior sensitivity and reproducibility compared to matrices commonly employed for lipids. A relatively small number of background peaks and good matrix suppression effect could make Ph-CCA-NH2 a widely applicable tool for lipid analysis.
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