Journal
ANALYTICAL CHEMISTRY
Volume 84, Issue 8, Pages 3849-3854Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac300414g
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Funding
- Global Research Laboratory of the National Research Foundation of Korea (NRF)
- Ministry of Education, Science, and Technology (MEST) of Korea [K20904000004-11A0500-00410]
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Droplet-based microfluidic systems have emerged as a powerful platform for performing high-throughput biological experimentation. In addition, fluorescence polarization has been shown to be effective in reporting a diversity of bimolecular events such as protein-protein, DNA-protein, DNA-DNA, receptor-ligand, enzyme-substrate, and protein-drug interactions. Herein, we report the use of fluorescence polarization for high-throughput protein-protein interaction analysis in a droplet-based microfluidic system. To demonstrate the efficacy of the approach, we investigate the interaction between angiogenin (ANG) and antiangiogenin antibody (anti-ANG Ab) and demonstrate the efficient extraction of dissociation constants (K-D = 10.4 +/- 3.3 nM) within short time periods.
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