Article
Agriculture, Multidisciplinary
Weronika Hewelt-Belka, Michal Mlynarczyk, Dorota Garwolinska, Agata Kot-Wasik
Summary: This study used reversed-phase liquid chromatography coupled to mass spectrometry to evaluate the molecular distribution of GM3 in human milk samples. The results showed that the types and content of GM3 varied across different lactation stages. These findings contribute to our understanding of the dynamic composition of human milk.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ying Zhang, Bei Gao, Luis Valdiviez, Chao Zhu, Tara Gallagher, Katrine Whiteson, Oliver Fiehn
Summary: Stable isotope tracers are commonly used with gas chromatography coupled to mass spectrometry for enzyme activity and metabolic pathway studies. In this research, three commercially available instruments were used to analyze samples from a stable isotope labeling study on Rothia mucilaginosa metabolism. The study found that all three GC-MS instruments can be used for stable isotope tracing studies, with high-resolution GC-QTOF MS offering additional capabilities in identifying the chemical structures of unknown compounds.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jun Young Park, Sarmila Amatya Shrestha, Sangwon Cha
Summary: The study investigated the feasibility of using reversed-phase liquid chromatography to separate ganglioside isomers, successfully achieving the isomer separation of disialogangliosides and trisialogangliosides for the first time. Crucial factors in the mobile-phase composition, such as the organic modifier acetonitrile and the ammonium salt additive ammonium bicarbonate, were identified to influence the differential retention and mass spectral response of the isomers.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Optics
Xinhu Fan, Youmin Rong, Guojun Zhang, Congyi Wu, Yuxuan Luo, Yu Huang
Summary: Ultrafast laser direct writing technology has been extensively studied in the fabrication of glass-based microfluidic devices. This study investigates the impact of scanning strategies and process parameters on the cross-sectional profile characteristics of microchannels. A new strategy, combining the offset method with the double bidirectional method, is proposed to achieve symmetrical cross-sectional profiles and smooth channel bottoms. The results demonstrate high-quality profiles of microchannels exhibit high efficiency and stability of flow in mixing experiments.
OPTICS AND LASER TECHNOLOGY
(2024)
Article
Chemistry, Analytical
Jing Chen, Fei Liu, Zhoumin Li, Li Tan, Mei Zhang, Danke Xu
Summary: A rapid and automated system for determination of aflatoxins in peanut oil was developed, integrating sample pretreatment into a microfluidic chip for the first time. Online mass spectrometry detection achieved with good linear calibration curves and low detection limits, demonstrating potential for application in various food samples.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
E. Mavrakis, Z. Toprakcioglu, N. Lydakis-Simantiris, T. P. J. Knowles, S. A. Pergantis
Summary: As the use of microfluidic chips for biological samples handling increases, there is a growing demand to combine them with powerful analytical techniques such as ICP-MS. This study demonstrates the successful coupling of a chip-based supersonic microfluidic nebulizer to an ICP-MS through the use of a spray chamber with a laminar flow makeup gas. The system shows promising performance in single-particle and single-cell analysis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Agricultural Engineering
Zainab Syed, Monika Sogani, Anu Kumar, Jayana Rajvanshi, Gopesh Sharma, Kumar Sonu
Summary: This research focused on the biodegradation of synthetic estrogenic compound 17 alpha-ethinylestradiol (EE2) and compared its degradation efficiency and power output in suspension flasks and a Bioelectrochemical System (BES). The results showed that the BES had significantly higher EE2 removal rate and power density. The study also identified a potential pathway for EE2 degradation using the newly isolated strain Rhodopseudomonas palustris MDOC01, and detected important metabolites during the bioelectrochemical treatment process of EE2.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Yan Zhang, Sungho Kim, Weihua Shi, Yaoyao Zhao, Insu Park, Christopher Brenden, Hrishikesh Iyer, Prasoon Jha, Rashid Bashir, Jonathan V. Sweedler, Yurii Vlasov
Summary: The silicon microfluidic platform allows for the monolithic integration of transparent micron-scale microfluidic channels, on-chip segmentation of analyte flows into picoliter droplets, and spatial and temporal separation of oil and aqueous phases during electro-spray for subsequent mass spectrometry analysis.
Article
Biochemical Research Methods
Fangfang Fan, Ning Xu, Yucheng Sun, Xuanhao Li, Xinchang Gao, Xizhen Yi, Yi Zhang, Xianli Meng, Jin-Ming Lin
Summary: This study elucidated a new mechanism of Salidroside in reducing hypoxia-induced inflammation in microglia by regulating cellular energy metabolism. The hypoxic microenvironment of BV2 microglial cells was simulated in vitro using a cell microfluidic chip-mass spectrometry (CM-MS) system, and the real-time change of cell metabolites was investigated. The levels of hypoxia-related factors were also detected to confirm the metabolic mechanism of BV2 cells under hypoxia. The study found that Salidroside could invert the changes in cellular energy metabolism and identified two metabolites of Salidroside.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Neurosciences
Masaya Ikegawa, Nobuto Kakuda, Tomohiro Miyasaka, Yumiko Toyama, Takashi Nirasawa, Karolina Minta, Jorg Hanrieder
Summary: This study applied MALDI-MSI to study the deposition of A beta peptides in AD brains, and found that different isoforms of A beta peptides have different distribution patterns. MALDI-MSI can also be used to study the lipid metabolism of plaques. This study provides a new method for understanding the pathogenesis of Alzheimer's disease.
BRAIN CONNECTIVITY
(2023)
Article
Environmental Sciences
Patricia Aguilar-Alarcon, Susana Gonzalez, Alexandros G. Asimakopoulos, Oyvind Mikkelsen
Summary: This study used quadrupole time-of-flight mass spectrometry (QTOF-MS) to characterize the molecular fingerprints of fulvic acids (RASFA) in recirculating aquaculture systems (RAS) and compared them with reference standards. The results demonstrated that QTOF-MS is a rapid and comprehensive screening technique for characterizing and monitoring differences in fulvic acid composition in RAS.
Article
Pharmacology & Pharmacy
Yingrui Zhang, Shiyu Chen, Fangfang Fan, Ning Xu, Xian-Li Meng, Yi Zhang, Jin-Ming Lin
Summary: This study investigates the mechanism of neurotoxicity induced by aconitine and finds that excitatory amino acids can trigger excitotoxicity. It is discovered that aconitine neurotoxicity is associated with the excitatory effects of glutamic acid and aspartic acid, which lead to the accumulation of lactic acid and reduction of glucose, ultimately resulting in cell apoptosis.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Shiyu Chen, Fangfang Fan, Yingrui Zhang, Jiuseng Zeng, Yuxuan Li, Ning Xu, Yi Zhang, Xian-Li Meng, Jin-Ming Lin
Summary: This study used a microfluidic-chip mass spectrometry method to investigate the drug metabolism changes of Scutellarin under hypoxia conditions. The system allowed real-time monitoring of metabolite changes and drug metabolism in BV2 cells. The findings shed light on the mechanism of Scutellarin in reducing hypoxia-induced injury and presented a novel strategy for monitoring the energy metabolic mechanism of drugs on cells.
Article
Chemistry, Analytical
Chu -Fan Wang, Liang Li
Summary: Chemical isotope labeling (CIL) LC-MS is a powerful tool for metabolome analysis that offers improved coverage and accuracy. This study compares quadrupole time-of-flight (QTOF) and Orbitrap mass spectrometers for metabolite detection in CIL LC-MS. The results show that instrument type has a significant effect on metabolite detection, and combining data from different instruments can increase the number of detected peak pairs.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Anke Kraemer, Peter Bauer, Felix. H. Schacher
Summary: A simple and robust reversed phase separation method was established for the separation of known impurities in bisphenol-A (BPA). The method offers shorter separation time and lower sample concentration requirements, making it suitable for frequent process monitoring. Additionally, four additional impurities were detected and identified.
JOURNAL OF CHROMATOGRAPHY A
(2022)