Article
Chemistry, Analytical
Ziyi He, Justin Huffman, Kathrine Curtin, Krista L. Garner, Elizabeth C. Bowdridge, Xiaojun Li, Timothy R. Nurkiewicz, Peng Li
Summary: The cPlate system, enabled by a novel microscale fluid manipulation method, allows for highly scalable multiplexed ELISA analysis with high sensitivity and specificity, shorter assay time, and lower consumption, making it suitable for point-of-care testing in resource-limited regions.
ANALYTICAL CHEMISTRY
(2021)
Article
Thermodynamics
Shuai Yuan, Mingyong Zhou, Xijiang Liu, Qiang Li, Bingyan Jiang
Summary: Microfluidic-based enrichment techniques have been extensively studied to enhance the sensitivity of detection by increasing the concentration of analytes. In this study, a numerical model is developed to investigate the complex analyte focusing process in a microfluidic electrophoresis chip. The results show that factors such as electrode location and the number of mass transfer channels are crucial for improving the enrichment performance. After considering the Joule heating effect, the final microchip achieves significant concentration enrichment. Moreover, the method is successfully applied to the experimental enrichment of Rhodamine B and cadmium (II) ions.
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
(2022)
Article
Chemistry, Analytical
Jianli Zhang, Alexander D. Wiener, Raymond E. Meyer, Cheuk W. Kan, David M. Rissin, Bharathi Kolluru, Christopher George, Carmen I. Tobos, Dandan Shan, David C. Duffy
Summary: This study presents methods to improve the quantification of digital bead assays (DBA), such as digital enzyme-linked immunosorbent assay (ELISA), which are widely used for highly sensitive protein measurement in clinical research and diagnostics. By changing the calculation method for the average number of enzymes per bead (AEB) and improving the determination of average product fluorescence intensity, the accuracy and robustness of DBA can be significantly improved.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Emory M. Payne, Maryam Taraji, Bridget E. Murray, Daniel A. Holland-Moritz, Jeffrey C. Moore, Paul R. Haddad, Robert T. Kennedy
Summary: Droplet microfluidics allows high-throughput experimentation by encapsulating samples in aqueous droplets. However, small molecules can transport between droplets, limiting the results obtained using fluorescent molecules. This study used ESI-MS to measure the transport of low molecular weight compounds between droplets and developed a predictive tool based on the analytes tested. The transport was found to depend on carrier fluids, surfactants, and flow conditions, and can be reduced through experimental design and surfactant tailoring.
ANALYTICAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Iiro Kiiski, Elisa Ollikainen, Sanna Artes, Paivi Jarvinen, Ville Jokinen, Tiina Sikanen
Summary: This study introduces a microfluidic reactor design using immobilized human liver microsomes to study UGT-mediated drug clearance under flow-through conditions. Characterization of the microreactor performance is done using glucuronidation of two model reactions. Conducting UGT metabolism assays under flow conditions facilitates in-depth mechanistic studies and may shed light on UGT latency.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Arian Yeganegi, Kaveh Yazdani, Nishat Tasnim, Somayeh Fardindoost, Mina Hoorfar
Summary: The utilization of microfluidic technology in gas sensors addresses challenges faced by traditional sensors, enhancing worker safety, mitigating environmental issues, and enabling early diagnosis of chronic diseases.
FRONTIERS IN CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Chemistry, Analytical
Jiani Yang, Xiaohua Xiao, Ling Xia, Gongke Li, Lingling Shui
Summary: The μMAD technique combines functionalized magnetic beads and a microfluidic device for ultra-sensitive biomarker detection and sample preparation, providing a valuable approach for trace-level biomarker detection in practical applications.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Dong Xiang, Ninghong Li, Lu Liu, Hengyi Yu, Xiping Li, Tinghui Zhao, Dong Liu, Xuepeng Gong
Summary: This study developed and validated ELISA-based methods for measuring infliximab and its ADA levels in human plasma, and successfully applied them in clinical therapeutic drug monitoring.
Article
Biochemical Research Methods
Anna Toldra, Georgios Chondrogiannis, Mahiar M. Hamedi
Summary: This paper presents a paper-based microfluidic device, called MEL-PAD, which can perform enzyme-linked assays. The MEL-PAD system utilizes a wash-free sandwich coupling and vertical flow to detect analytes. It achieves efficient washing and color change detection using nitrocellulose and a chromogenic substrate. The MEL-PAD demonstrates high sensitivity and versatility for quantifying various analytes. The article also shows the potential of MEL-PAD in detecting DNA from Staphylococcus epidermidis.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Medicine, General & Internal
Steev Loyola, Alfredo Huaman, Dina Popuche, Elizabeth Castillo, Julia S. Ampuero, Maria Silva, Carolina Guevara, Douglas M. Watts
Summary: The study evaluated the performance of MAC-ELISA and PRNT for diagnosing ZIKV infection in dengue-endemic areas, with both assays showing high sensitivity and specificity. Restricting the analysis according to neutralizing antibodies against dengue or IgM antibodies, the performances of both serological assays were adequate.
Article
Chemistry, Analytical
Jiani Yang, Ling Xia, Gongke Li
Summary: A spatial confined enzyme-linked immunoassay using a microfluidic magnetic delivery disc has been developed for ultrasensitive biomarker detection. The method offers high sensitivity and accuracy with magnetic preconcentration and fluorescence detection in a confined space.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Microbiology
Alex Moskaluk, Mary Nehring, Sue VandeWoude
Summary: The study evaluated the specificity of ELISA for detecting FIV and FFV infections in domestic cats, showing that exposure to off-target pathogens may affect the test results. SPF cat sera experimentally infected with the corresponding virus had lower background in ELISA, while FIV-specific background absorbance was higher than that of FFV.
Article
Veterinary Sciences
Kaori Murata, Miho Nagasawa, Tatsushi Onaka, Ken-ichi Takeyama, Takefumi Kikusui
Summary: Research on oxytocin has shown that the newly developed ELISA method is comparable in urine samples, but not applicable in serum. Further modifications are needed for its application in serum oxytocin detection.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2021)
Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Biochemical Research Methods
Ling Xia, Naoki Yanagisawa, Rajesh Deb, Debashis Dutta
Article
Biochemical Research Methods
Tristan F. Kinde, Natalie Hess, Debashis Dutta
Article
Plant Sciences
Hiroki Tsutsui, Naoki Yanagisawa, Yaichi Kawakatsu, Shuka Ikematsu, Yu Sawai, Ryo Tabata, Hideyuki Arata, Tetsuya Higashiyama, Michitaka Notaguchi
Article
Chemistry, Analytical
Naoki Yanagisawa, Sakur Mahmud, Debashis Dutta
ANALYTICAL CHEMISTRY
(2020)
Article
Biochemical Research Methods
Yukari Liu, Ling Xia, Debashis Dutta
Summary: This article introduces a microchip device that generates pressure gradients on-chip via electrokinetic means to minimize dead volume in the system, allowing pressure-driven flow to introduce sample zones into a CZE channel with minimal injection bias. This injection technique enabled the introduction of narrow sample plugs with spatial standard deviations down to about 45 μm, and was integrated into a capillary zone electrophoresis process for analyzing amino acid samples with separation resolutions of about 4-6 for the analyte peaks in a 3 cm long analysis channel.
Article
Microbiology
Sayumi Fukuda, Riho Yamamoto, Naoki Yanagisawa, Naoki Takaya, Yoshikatsu Sato, Meritxell Riquelme, Norio Takeshita
Summary: The tip-growing fungal cells elongate by de novo synthesis of the cell wall while maintaining cell polarity at the apical regions. How cell polarity and growth rate cooperate to determine cell shape remain unclear. Analysis of hyphal tip growth of filamentous fungi growing inside narrow channels revealed a trade-off between plasticity and velocity in mycelial growth, with fast-growing hyphae often losing polarity after emerging from the channels and slow-growing hyphae retaining polarity. These results contribute to understanding fungal invasive growth and its impact on biotechnology, ecology, and pathogenicity.
Review
Plant Sciences
Naoki Yanagisawa, Elena Kozgunova, Guido Grossmann, Anja Geitmann, Tetsuya Higashiyama
Summary: Microfabrication techniques and microfluidic platforms have been increasingly used in studying plant cells and organs, providing new tools and methods for investigating plant growth processes.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Chemistry, Physical
Takayuki Ikeda, Takahisa Okubo, Juri Saruta, Makoto Hirota, Hiroaki Kitajima, Naoki Yanagisawa, Takahiro Ogawa
Summary: The study investigated the impact of storage and temperature fluctuations on the bioactivity of titanium implants. It was found that stored samples were more hydrophobic compared to fresh samples, which significantly affected osteoblast attachment. Maintaining the samples at 31 degrees Celsius was shown to maximize cell attachment. UV treatment was effective in restoring bioactivity compromised by temperature fluctuations.
Article
Mechanics
Debashis Dutta
Summary: This article presents a mathematical formulation for quantitating band broadening in msACE systems due to pressure-driven flow, reducing errors in estimating parameter values.
Article
Biochemical Research Methods
Basant Giri, Debashis Dutta
Summary: This study aims to implement multiplex ELISA in microchannels with a smaller footprint and sample volume requirement. Selective coating of target molecules is achieved using electroosmosis, allowing for shorter channel dimensions and additional functional enhancements.
Article
Chemistry, Analytical
Ling Xia, Rajesh Deb, Naoki Yanagisawa, Debashis Dutta
Summary: This article reports significant improvements in the resolving power of pressure-driven charge-based separations performed in sub-micrometer deep glass channels upon introducing an electrokinetic backflow. The optimization of operating conditions and the use of suitable electrokinetic backflow strategy can greatly enhance the separation resolution.
ANALYTICA CHIMICA ACTA
(2022)
Article
Mechanics
Naoki Yanagisawa, Victor Dominguez, Sakur Mahmud, Debashis Dutta
Summary: This manuscript presents a visualization study of evaporation-driven capillary flow in an electrokinetic energy conversion device. The liquid velocity and evaporation rate are found to be correlated with the generated voltage and current. The results provide insights for the development of efficient energy conversion platforms.
Review
Biochemical Research Methods
Sakur Mahmud, Sarker Ramproshad, Rajesh Deb, Debashis Dutta
Summary: The broadening of analyte streams in free-flow electrophoresis (FFE) separations limits the resolving power. This is caused by diffusion perpendicular to the streamflow direction and variations in analyte migration. Factors including Joule heating and pressure-driven crossflows contribute to the broadening. Recent studies have investigated these contributions and discussed areas for further research in better understanding and minimizing zone broadening in FFE assays.
Article
Chemistry, Multidisciplinary
Naoki Yanagisawa, Elena Kozgunova, Tetsuya Higashiyama
Summary: This study presents a microfluidic injector device coupled with a cell culture chamber to study directional cellular growth triggered by ligand-receptor interactions at single molecule resolution. The unidirectional fluidic flow introduced by a syringe pump, along with an on-chip electro-osmotic pump for temporal change in flow direction, allows for controlled transfer of sample plugs into the cell culture chamber. This setup potentially enables study of cellular responses to injected ligands at high spatiotemporal resolution.