Article
Chemistry, Analytical
Andrey Krutilin, Sascha W. Epp, Glaynel M. L. Alejo, Frederik Busse, Djordje Gitaric, Hendrik Schikora, Heinrich Schwoerer, Friedjof Tellkamp
Summary: We developed a cryogenic mass spectrometry method using femtosecond laser pulses to detect peptides in ice films at the attomole dilution range. Our study found that temperature, substrate composition, and irradiation wavelength had minimal impact on signal intensity once the preparation protocol was optimized. We observed a complex behavior of analyte desorption and ionization with thinning ice films at increased temperatures. Cryogenic conditions enabled signal detection only from isolated spots, while higher temperatures led to more frequent detection and intense signal onset. Additionally, we found that the fragmentation pattern produced by both irradiation wavelengths was similar. Our findings highlight the potential of femtosecond mass spectrometry for analyzing small organic molecules and peptides in aqueous solution without the need for matrix or substrate surface modification.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Article
Chemistry, Analytical
Xufang Hu, Zongping Wang, Haolin Chen, An Zhao, Nianrong Sun, Chunhui Deng
Summary: An advanced metabolic analysis tool based on highly porous metal oxides derived from metal-organic frameworks (MOFs) is developed in this study. The tool successfully differentiates and stages renal cell carcinoma (RCC), as well as identifies the size of tumors. This achievement shows promising prospects for designer matrix-based analytical tools in disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Katerina V. Djambazova, Jacqueline M. Van Ardenne, Jeffrey M. Spraggins
Summary: Imaging mass spectrometry allows for the untargeted mapping of biomolecules directly from tissue sections, with widespread clinical applicability in disease diagnosis and classification. This review summarizes the two most widely employed IMS technologies and their advancements in biomedical applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Infectious Diseases
Margarita Estreya Zvezdanova, Pilar Escribano, Jesus Guinea, Patricia Munoz, David Rodriguez-Temporal, Belen Rodriguez-Sanchez
Summary: The implementation of commercial kits for standardized fungal sample preparation and updated reference libraries enables the easy use of MALDI-TOF MS for routine identification of fungal species. Although further improvement in the databases is needed, this method allows for the correct identification of a significant number of fungal species at the species level.
Article
Biochemical Research Methods
Ari Chae, Gwanwon Lee, Dong-Yeun Koh, Cheol-Min Yang, Sungho Lee, Young-Kwan Kim
Summary: Carbon fiber (CNF), prepared from electrospun PAN fibers, shows high salt tolerance, sensitivity, and resolution for matrix-free LDI-MS analysis, making it a promising material for developing an efficient and universal platform for analyte analysis regardless of ionization modes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
R. Gathercole, E. Tranfield, D. Xia, G. Perez-Cordon, G. Robinson, D. Timofte, F. Zendri, R. M. Chalmers
Summary: The study developed a method to purify Cryptosporidium spp. oocysts from clinical stool samples and evaluated them using mass spectrometry. The method produced distinct spectra specific to different Cryptosporidium species, demonstrating the utility of the purification method.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Review
Microbiology
Xin-Fei Chen, Xin Hou, Meng Xiao, Li Zhang, Jing-Wei Cheng, Meng-Lan Zhou, Jing-Jing Huang, Jing-Jia Zhang, Ying-Chun Xu, Po-Ren Hsueh
Summary: MALDI-TOF MS has been used in clinical microbiology since 2010, offering advantages such as convenience, speed, accuracy, and low cost. It has shown promising results in rapid detection of highly virulent and drug-resistant pathogens, making it a valuable tool in clinical microbial identification.
Article
Infectious Diseases
Jichan Shi, Gexin Gao, Jing Pan, Lian-Peng Wu, Hongye Ning, Zhengxin Wu, Xinchun Ye, Xiangao Jiang
Summary: This study evaluated the clinical value of MALDI-TOF MS in detecting NTM by comparing the results with liquid culture and gene chip. The findings showed that MALDI-TOF MS had a high consistency with both methods and could be a rapid and effective method for identifying NTM strains and drug resistance.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Polymer Science
Ulric Conway, Alexander D. Warren, Christopher J. Arthur, Paul J. Gates
Summary: The main goals in the analysis of synthetic polymers include determining molecular weight, molecular weight distribution, and chemical structure. Matrix-assisted laser desorption/ionization mass spectrometry is a commonly used technique, and using carbon-based matrices with polyethylene glycol polymers doped with LiCl is an effective method for analyzing low molecular weight polymers.
Article
Chemistry, Analytical
Huifang Zhao, Huayu Zhao, Jie Wang, Jianying Ren, Jia Yao, Yanqiu Li, Ruiping Zhang
Summary: In this study, a novel interfacial carbon-based nanocomposite (Ni/N-CNT/rGO) was synthesized as a matrix for gastric cancer saliva metabolic analysis. Using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOFMS), four potential biomarkers were successfully screened from saliva, leading to an accuracy of 92.50%, specificity of 88.03%, and sensitivity of 97.12% for distinguishing gastric cancer patients from healthy controls.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Guanhua Zhang, Chunxia Ma, Qing He, Hongjing Dong, Li Cui, Lili Li, Lingyu Li, Yan Wang, Xiao Wang
Summary: In this study, Pt@MXene was synthesized as an ideal substrate for SALDI MS, and it showed excellent performance for the high-throughput detection of small-molecule natural products. It provided stronger signal intensity, wider molecular coverage, lower background interference, good salt and protein tolerance, good repeatability, and high detection sensitivity. The Pt@MXene substrate was also successfully used for quantifying target molecules in medicinal plants. This proposed method has great potential for wide applications.
Article
Biochemical Research Methods
Tanja Bien, Elizabeth A. Hambleton, Klaus Dreisewerd, Jens Soltwisch
Summary: The Waminoa sp. acoel flatworms hosting Symbiodiniaceae and Amphidinium dinoflagellate algae provide an interesting model for symbiosis in marine environments. Through advanced imaging techniques, it was discovered that different sterol species play specific roles in the metabolism of Waminoa beyond simply serving as a source of energy, highlighting the value of this research for future spatially resolved analysis of sterols.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Tanja Bien, Sebastian Bessler, Klaus Dreisewerd, Jens Soltwisch
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has the potential to visualize the spatial distribution of biomolecules in cell cultures and tissue sections with high resolution, but sample preparation is crucial. A study found that a robust sample preparation protocol can preserve cell morphology and molecular integrity, contributing to the study of cell-to-cell heterogeneities at a molecular level. Advancements in single-cell MALDI imaging could benefit from these protocols.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Eike U. Brockmann, Alexander Potthoff, Sara Tortorella, Jens Soltwisch, Klaus Dreisewerd
Summary: UV-MALDI-MSI is a powerful tool for visualizing bacterial metabolites in microbial colonies and biofilms. The use of a pulsed IR laser without prior matrix coating allowed for efficient extraction of analytes, utilizing water or glycerol for energy deposition and ionization. This approach provided enhanced detection of small molecules and bacterial phospholipids, demonstrating potential as a new tool for microbial mass spectrometry imaging.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Alexander Potthoff, Olaf Minte, Klaus Dreisewerd, Jens Soltwisch
Summary: This study investigates the ionization mechanisms of postionization (PI) in MALDI-2 and conducts experiments using two lasers with different pulse durations. The results show that the ion signal intensity has a quadratic dependency on the pulse energy, and shorter pulses produce higher ion signal intensities due to higher photon density. Comparative measurements allow the determination of the excited state lifetime of the matrices used.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Antonin Bednarik, Vadym Prysiazhnyi, Dominika Bezdekova, Jens Soltwisch, Klaus Dreisewerd, Jan Preisler
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Christoph Bookmeyer, Ulrich Roehling, Klaus Dreisewerd, Jens Soltwisch
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a rapidly growing method in the life sciences. The study introduces a novel post-ionization scheme based on single-photon induced chemical ionization using pulsed RF-Kr lamps, which effectively improves the ionization efficiency for analytes. Through the interplay with chemical dopants, the signal of various lipid molecules from mammalian tissue sections was significantly boosted.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Multidisciplinary Sciences
Tanja Bien, Krischan Koerfer, Jan Schwenzfeier, Klaus Dreisewerd, Jens Soltwisch
Summary: Molecular analysis on the single-cell level is rapidly growing in the life sciences. This study presents a sensitive approach to single-cell mass spectrometry based on high-resolution imaging and optical microscopy. The multimodal datasets allow for statistical analysis and direct connection of MS data with microscopy information, providing insights into intracellular lipid distributions and subcellular molecular profiles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Analytical
Sebastian Bessler, Jens Soltwisch, Klaus Dreisewerd
Summary: This study used mass spectrometry imaging to investigate the distribution of cardiolipin in murine retinal cell layers and found that differentially enriched and expressed polyunsaturated fatty acids play an important role in retinal pathologies.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Patric Bourceau, Benedikt Geier, Vincent Suerdieck, Tanja Bien, Jens Soltwisch, Klaus Dreisewerd, Manuel Liebeke
Summary: Label-free molecular imaging techniques can directly map hundreds of metabolites in biological tissues, but it is difficult to localize microbes and assign metabolites to host or microbiome members in host-microbe interactions. To solve this problem, we developed a correlative imaging approach combining MALDI-MSI with FISH to identify and localize microbial cells on the same section.
Article
Chemistry, Multidisciplinary
C. L. Logan Mackay, Jens Soltwisch, Bram Heijs, Karl W. Smith, Faye L. Cruickshank, Annika Nyhuis, Klaus Dreisewerd, Diego Cobice
Summary: Prostate cancer is usually treated with androgen deprivation therapy, which can be limited by the emergence of a more lethal 'castrate resistant' form of the disease. Intracrine pathways may generate growth-stimulating androgens, and mass spectrometry imaging allows for visualization of biomolecules in tissues.