Article
Biochemistry & Molecular Biology
Ching-Chia Wang, Huang-Jen Chen, Ding-Cheng Chan, Chen-Yuan Chiu, Shing-Hwa Liu, Kuo-Cheng Lan
Summary: The study found that acrolein exposure led to increased fasting blood glucose and impaired glucose tolerance in mice. It also showed that acrolein treatment decreased the expression of GLUT4 in both mouse soleus muscles and C2C12 myotubes, as well as suppressed phosphorylation of glucose metabolic signals. Additionally, over-expression of constitutive activation of Akt reversed the inhibitory effects of acrolein on GLUT4 protein expression and glucose uptake in myotubes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Clothilde Philouze, Sophie Turban, Beatrice Cremers, Audrey Caliez, Gwladys Lamarche, Catherine Bernard, Nicolas Provost, Philippe Delerive
Summary: The research findings suggest that MG53 is not a critical regulator of insulin signaling pathway in skeletal muscle, as demonstrated by in vitro and in vivo experiments.
Article
Endocrinology & Metabolism
Paola A. Ramos, Kelli A. Lytle, Danae Delivanis, Soren Nielsen, Nathan K. LeBrasseur, Michael D. Jensen
Summary: The study found that there were no defects in insulin receptor signaling at the Akt/AS160 level in the muscles of obese individuals, indicating uncertainties in the mechanism of muscle insulin resistance in obesity.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Jeane Rebecca Roy, Coimbatore Sadagopan Janaki, Selvaraj Jayaraman, Vijayalakshmi Periyasamy, Thotakura Balaji, Madhavan Vijayamalathi, Vishnu Priya Veeraraghavan
Summary: This study evaluated the effect of Carica papaya leaf extract on high fat diet-induced type 2 diabetic rats and identified its potential mechanism of action against insulin resistance. The results showed that Carica papaya treatment improved blood glucose levels, lipid profile, serum insulin, and tissue antioxidant enzymes. Molecular docking and dynamics analysis identified kaempferol, quercitin, and transferulic acid as the top ligands with the greatest hit rate against the insulin receptor and glucose transporter-4 protein targets. This study suggests that Carica papaya could reinstate glycemic control in diabetic skeletal muscle.
Article
Biochemistry & Molecular Biology
Rekha Balakrishnan, Pablo A. Garcia, Rajakrishnan Veluthakal, Janice M. Huss, Joseph M. Hoolachan, Debbie C. Thurmond
Summary: The study found that PAK1 not only plays a role in insulin-stimulated glucose uptake, but also regulates skeletal muscle mitochondrial function. When PAK1 levels decrease, mitochondrial copy number and respiration decrease, mitochondrial structure changes, and regulators PGC1α and PGC1α activators are also downregulated. However, PAK1 enrichment can improve mitochondrial function in insulin-resistant myotubes and rescue the expression levels of PGC1α. These results reveal that PAK1 regulates muscle mitochondrial function through the p38MAPK/ATF2/PGC1α pathway.
Article
Biochemistry & Molecular Biology
Praveen Kumar Issac, Rupmanjari Karan, Ajay Guru, R. Pachaiappan, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Ki Choon Choi, Ramasamy Harikrishnan, Jesu Arockia Raj
Summary: Morin acts as an antioxidant by reducing ROS levels and demonstrating insulin mimetic activity, reversing insulin resistance associated with oxidative stress.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Aiwen Jiang, Hongyun Guo, Xiaoyu Jiang, Jingli Tao, Wangjun Wu, Honglin Liu
Summary: This study reveals the down-regulation of G6PD during skeletal muscle development and explores its importance in myogenic differentiation. G6PD deficiency may provide a new perspective for the treatment of insulin resistance and type-2 diabetes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Man Piu Chan, Nobuyuki Takenaka, Takaya Satoh
Summary: Insulin-stimulated glucose uptake in skeletal muscle is impaired in Lep(ob/ob) mouse model due to the complete inhibition of Akt2-mediated activation of Rac1 and partial inhibition of RalA activation downstream of Rac1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Endocrinology & Metabolism
Jinhee Hwang, Debbie C. Thurmond
Summary: The impaired GLUT4 translocation and defects in SNARE proteins and their regulators are closely related to the pathogenesis of diabetes. Novel functions of SNARE proteins in pathways not typically associated with SNAREs may provide new therapeutic targets for combating insulin resistance and diabetes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jongsoo Mok, Tae Sub Park, Sunhong Kim, Daehoon Kim, Cheol Soo Choi, Joonghoon Park
Summary: The study found that Prokr1 plays an important role in skeletal muscle, improving insulin sensitivity and being a potential therapeutic target for ameliorating insulin resistance in skeletal muscle.
Article
Pharmacology & Pharmacy
Huimin Wang, Xuepei Lei, Zhuo Zhang, Mohammad Mehdi Ommati, Zhongwei Tang, Jianqin Yuan
Summary: The study indicates that BPA exposure has detrimental effects on cognitive function and insulin signaling pathway in male offspring mice, potentially posing a risk for long-term neurodegeneration.
Article
Food Science & Technology
Peiyuan Li, Ruilin Xu, Yanfei Shi, Xiaoyang Shi, Xinyuan Zhang, Jinjie Li, Guangning Kou
Summary: This study found that luteolin can increase the proportion of slow myofibers and improve skeletal muscle fitness by activating the FLCN-AMPK-PGC-1α pathway.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemistry & Molecular Biology
Zhongyang Guo, Xiaoling Chen, Daiwen Chen, Bing Yu, Jun He, Ping Zheng, Yuheng Luo, Hong Chen, Hui Yan, Zhiqing Huang
Summary: In this study, porcine myotubes derived from skeletal muscle satellite cells were used to investigate the effects of DHM on myosin heavy chain (MyHC) expression and its mechanism. The results showed that DHM up-regulated MyHC I protein expression and down-regulated MyHC IIb protein expression through the AMPK signaling pathway. DHM also affected the activities of certain enzymes and increased the expression of AMPK-related factors.
Article
Cell Biology
Zheng Fan, Guillermo Turiel, Raphaela Ardicoglu, Moheb Ghobrial, Evi Masschelein, Tea Kocijan, Jing Zhang, Ge Tan, Gillian Fitzgerald, Tatiane Gorski, Abdiel Alvarado-Diaz, Paola Gilardoni, Christopher M. Adams, Bart Ghesquiere, Katrien De Bock
Summary: The study suggests that there are two populations of muscle endothelial cells characterized by differential expression of ATF3/4, with red ATF3/4(+) mECs being more angiogenic compared to white ATF3/4(low) mECs. Mechanistically, ATF3/4 in mECs control genes involved in amino acid uptake and metabolism, metabolically priming red mECs for angiogenesis. Deletion of Atf4 in ECs was found to impair exercise-induced angiogenesis.
Article
Agriculture, Multidisciplinary
Guangning Kou, Peiyuan Li, Yanfei Shi, Stanislav Seydou Traore, Xiaoyang Shi, Adwoa Nyantakyiwaa Amoah, Zhenwei Cui, Quanjun Lyu
Summary: Sesamin, a major component in sesame, has been found to activate FNDC5/irisin and regulate the SIRT1/SMAD3 signaling pathway, leading to improved obesity and skeletal muscle dysfunction.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Physiology
Rie Mukai, Hitomi Horikawa, Pei-Yi Lin, Nao Tsukumo, Takeshi Nikawa, Tomoyuki Kawamura, Hisao Nemoto, Junji Terao
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2016)
Article
Nutrition & Dietetics
Chiemi Kamada, Rie Mukai, Akari Kondo, Shinya Sato, Junji Terao
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
(2016)
Article
Biochemistry & Molecular Biology
Rie Mukai, Naoko Matsui, Yutaka Fujikura, Norifumi Matsumoto, De-Xing Hou, Noriyuki Kanzaki, Hiroshi Shibata, Manabu Horikawa, Keiko Iwasa, Katsuya Hirasaka, Takeshi Nikawa, Junji Terao
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2016)
Article
Food Science & Technology
Ayami Hisanaga, Rie Mukai, Kozue Sakao, Junji Terao, De-Xing Hou
MOLECULAR NUTRITION & FOOD RESEARCH
(2016)
Article
Biochemistry & Molecular Biology
Rie Mukai
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2018)
Article
Nutrition & Dietetics
Agustin Martin Morales, Rie Mukai, Kaeko Murota, Junji Terao
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
(2018)
Article
Plant Sciences
Takahito Takase, Satoshi Ikeuchi, Takashi Inoue, Rie Mukai
Summary: The study found that lemon peel extract containing eriocitrin can suppress muscle atrophy and reduce the expression of ubiquitin ligase genes through its antioxidative effect, preventing disuse muscle atrophy caused by oxidative stress.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Chemistry, Analytical
Naoya Fuse, Masashi Matsui, Yukako Matsue
Summary: This study used Fourier-transform infrared (FT-IR) microscopy to examine the real-time water behavior in untreated and bleached hair. The results showed that the peak intensity of water penetration in untreated hair was higher than that in bleached hair, and heavy water evaporated slower in untreated hair. These differences may be attributed to the breaking of chemical bonds caused by bleaching treatment.
ANALYTICAL SCIENCES
(2022)
Article
Food Science & Technology
Yoshiaki Tanaka, Hitomi Okuyama, Miyu Nishikawa, Shin-ichi Ikushiro, Mayumi Ikeda, Yu Ishima, Yuichi Ukawa, Kenichi Oe, Junji Terao, Rie Mukai
Summary: 8-Prenylnaringenin (8-PN) is a hop flavonoid with promising health benefits, exhibiting stronger estrogenic activity and prevention of muscle atrophy compared to nonprenylated naringenin. It also prevents hot flushes and bone loss. The bioavailability parameters of 8-PN and naringenin, such as pharmacokinetics, tissue distribution, binding affinity to human serum albumin, and cellular uptake in HEK293 cells, were compared. The results showed that 8-PN had higher amounts in various tissues and higher binding affinity to HSA and cellular uptake compared to naringenin. The bioavailability of 8-PN was distinct from that of naringenin in mouse or human model experiments.
FOOD SCIENCE & NUTRITION
(2022)
Article
Chemistry, Analytical
Naoya Fuse, Yukako Matsue, Shigeaki Morita
Summary: A method for predicting hair breakage induced by change of molecular structure was proposed in this study. Infrared spectroscopy, coupled with multivariate curve resolution and two-dimensional correlation spectroscopy, was used to investigate the changes inside hair caused by cyclical extension. The experiments revealed that cyclical extension in bleached hair increased the signal of certain functional groups, leading to hair breakage. The agreement between the CH2 profile and the component profile suggested the translocation of lipids inside hair caused by cyclical extension. In contrast, untreated hair showed gradual increase in CH2 signal without breakage. By monitoring the functional groups in hair, this method has potential application in predicting hair breakage caused by cyclical extension.
ANALYTICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nao Fujiwara, Rie Mukai, Miyu Nishikawa, Shinichi Ikushiro, Akira Murakami, Akari Ishisaka
Summary: This study quantified quercetin and its metabolites in the breast milk, plasma, urine, and neonatal mice of quercetin-fed maternal mice. Interestingly, the concentration of quercetin aglycone in the milk was much higher than in the maternal mice's plasma, suggesting potential biological activity in neonates.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Erika Nuka, Masako Takahashi, Masami Okitsu, Chisako Nayama, Honomi Nishijima, Ryutaro Sogawa, Kyuichi Kawabata, Junji Terao, Rie Mukai
Summary: The combined intake of cooked sweet potato and fried onion in humans has been found to suppress the increase of plasma quercetin metabolite concentration. Experiments with rat beta-glucosidase suggest that excess carbohydrate digestion products, especially glucose-containing saccharides, interfere with the deglycosylation of quercetin glucosides during their uptake in the intestinal epithelium. Therefore, combined meals of sweet potato and onion may lower the bioavailability of quercetin glucosides in onion.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Rie Mukai, Takashi Fukuda, Asami Ohnishi, Takeshi Nikawa, Mutsuki Furusawa, Junji Terao
Summary: The study evaluated the food matrix effects of chocolate on the absorption of green tea catechins in five healthy women. It was found that the plasma profiles of ECg were significantly affected by the chocolate matrix, with ECg persisting in the plasma for a longer period compared to EGCg.