Article
Chemistry, Analytical
Tibo De Saegher, Joeri Vercammen, Boyana Atanasova, Kevin M. Van Geem, Jeriffa De Clercq, An Verberckmoes, Jeroen Lauwaert
Summary: This study enhances the potential of GPC-HPLC-UV/VIS technique for the analysis of lignin depolymerization product pools, enabling fast yet sufficiently in-depth analysis of a large volume of samples. This capability is crucial for catalyst screening and fine-tuning reaction conditions.
ANALYTICA CHIMICA ACTA
(2023)
Article
Energy & Fuels
Jian-Fang Xu, Fei Wang, Xing Fan, Turghun Muhammad, Peter N. Kuznetsov, Ya-Ya Ma
Summary: This study investigated the thermal dissolution process of soft coal and hard coal using different organic solvents. By employing off-line prep-HPLC, the soluble fractions were reliably separated and enriched, leading to the identification of heavy aromatic compounds not detected by GC/MS. The differences in composition between the two coals were elucidated, providing theoretical guidance for efficient coal utilization and mine safety.
Article
Environmental Sciences
Kleopatra Miserli, Christina Kosma, Ioannis Konstantinou
Summary: Pharmaceuticals released from wastewater treatment plants are emerging contaminants that can have biological effects at low concentrations. Conventional wastewater treatment methods are unable to completely remove these contaminants, leading to their presence in sewage sludge. This study developed a method to detect pharmaceuticals in sewage sludge and hydrochar using a combination of extraction and chromatography techniques. The method was successful in detecting various pharmaceutical compounds in the samples tested.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Miguel Esperanca-Martins, Iola F. Duarte, Mara Rodrigues, Joaquim Soares do Brito, Dolores Lopez-Presa, Luis Costa, Isabel Fernandes, Sergio Dias
Summary: The prognosis of soft tissue sarcomas (STS) is disappointing due to the lack of individualized treatment strategies and the neglect of specificities and genetic/metabolic differences among sarcoma subtypes. There is a need for more studies to decipher the mechanisms of sarcomagenesis, improve STS diagnosis precision, refine STS classification criteria, and identify prognostic biomarkers. Metabolomics, either as a single-omics approach or as part of a multi-omics strategy, provides valuable insights into the metabolic adaptations that support sarcomagenesis. Further studies employing various data acquisition methods have provided important information for the characterization of the STS metabolome. New methods based on chromatography, mass spectrometry (MS), nuclear magnetic resonance (NMR) spectroscopy, and flow cytometry will offer opportunities to target metabolic pathways therapeutically and monitor the response to such therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Jungyeon Kim, Inho Jung, Yu Eun Cheong, Kyoung Heon Kim
Summary: Cofactors are crucial in the metabolism of living organisms, and understanding their concentration and extraction methods is essential. This study systematically compares and suggests optimal LC/MS methods for cofactor analysis and extraction methods from Saccharomyces cerevisiae.
ANALYTICA CHIMICA ACTA
(2022)
Article
Dermatology
Qiying Xiong, Daoqing Zhong, Qian Li, Yihui Yu, Sanquan Zhang, Jingyao Liang, Xibao Zhang
Summary: Recent studies have found that certain metabolites in the blood and urine are involved in the development of psoriasis, but the research on skin metabolomics for psoriasis is limited. In this study, researchers compared the metabolic profiles of lesional and nonlesional skin from 12 psoriasis patients using liquid chromatography-mass spectrometry. They identified several metabolites that were significantly different between lesional and nonlesional skin, mainly related to amino acid, lipid, and nucleotide metabolism. Fourteen potential biomarkers were identified, some of which were correlated with disease severity.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Chemistry, Applied
Christina Kosma, Ourania L. Koloka, Triantafyllos A. Albanis, Ioannis K. Konstantinou
Summary: A sensitive and rapid method for the determination of multiclass pesticides in white and red wines was developed using QuEChERS extraction procedure and UHPLC-Orbitrap-MS. The method was optimized to improve recoveries and reduce co-extractives, and successfully applied to real samples, revealing relatively low levels of two selected fungicides.
Article
Physiology
Eden A. Houske, Matthew G. Glimm, Annika R. Bergstrom, Sally K. Slipher, Hope D. Welhaven, Mark C. Greenwood, Greta M. Linse, Ronald K. June, Alan S. L. Yu, Darren P. Wallace, Alyssa K. Hahn
Summary: This study analyzed the urine metabolites of early-stage ADPKD patients and identified metabolic pathway alterations and candidate diagnostic and prognostic biomarkers. The results provide potential therapeutic targets and biomarkers for early diagnosis and tracking disease progression of ADPKD.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ourania Koloka, Marioanna Koulama, Dimitra Hela, Triantafyllos Albanis, Ioannis Konstantinou
Summary: A multi-residue method involving modified QuEChERS extraction procedure, sonication, and UHPLC-HR-Orbitrap-MS was developed to detect pharmaceutical drug residues in full-fat milk. The optimized method showed good linearity, recovery, precision, and acceptable matrix effects and expanded uncertainty. The method was successfully applied to real milk samples, revealing the presence of imidocarb in one sample.
Article
Biochemical Research Methods
Ran Kong, Junbo Zhao, Wenya Zhai, Zhuonan Chen, Shuo Yang, Mobing Chen, Jiaman Lin, Lina Wu, Wanhui Liu, Ping Xiang
Summary: Clonazolam metabolism was studied using in vivo and in vitro samples, with liquid chromatography-quadrupole/electrostatic field orbitrap mass spectrometry. Several metabolites were detected in both zebrafish model and human liver microsomes. The main types of metabolic reactions of clonazolam were identified. The results provide a theoretical basis for the detection and forensic medicine of clonazolam abuse.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Serkan Topal, Miray Oner, Buse Tugba Zaman, Cansu Demir, Fatma Turak, oemer Tahir Gunkara, Sezgin Bakirdere
Summary: An easy and rapid derivatization and preconcentration method called vortex assisted dispersive liquid-liquid microextraction (VA-DLLME) was proposed for the determination of selenium in lake water samples. The gas chromatography-mass spectrometry (GC-MS) system was utilized to determine inorganic selenium through a developed derivatization procedure. The VA-DLLME method enhanced the detection capability of the GC-MS system through preconcentration of the analyte. Under optimized conditions, the VA-DLLME-Se-PDA-FVD-GC-MS method showed a LOD of 2.2 ng mL(-1) and a LOQ of 7.3 ng mL(-1). Spiking experiments with lake water confirmed the accuracy and applicability of the method with a percent recovery range of 80-106%.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Applied
Suo Decheng, fan xia, Xiao Zhiming, Wei Shulin, Wang Shi, Wang Peilong
Summary: The method developed enables rapid screening and quantification of progesterone and progestins in milks using UHPLC QE HF HRMS. It meets the validation requirements for veterinary drug residue detection in the EU and China, and has been successfully applied to detect trace levels of progesterone and monitor residual progestins in real milk.
Article
Chemistry, Analytical
Deniss Fedorenko, Antons Podjava, Arvis Prikulis, Vadims Bartkevics
Summary: A new method using nanoflow liquid chromatography with Orbitrap mass spectrometry has been developed for quantitative analysis of biomarkers and pharmaceutical compounds in wastewater. The method employs an easy dilute-and-shoot approach for sample preparation and utilizes tetrabutylammonium bromide as an ion pair reagent to improve the retention of ionic and highly polar compounds. The new method demonstrates low matrix effects, high sensitivity, low injection volume and solvent consumption, and the ability to analyze diverse polar and ionic analytes within one run.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Oncology
Niklas Kehl, Katja Schlichtig, Pauline Duerr, Laura Bellut, Frank Doerje, Rainer Fietkau, Marianne Pavel, Andreas Mackensen, Bernd Wullich, Renke Maas, Martin F. Fromm, Arne Gessner, R. Verena Taudte
Summary: Oral anticancer drugs have significantly improved outcomes in the treatment of various tumors. However, variability in plasma concentrations of oral anticancer drugs in patients undergoing therapy poses risks of reduced therapeutic effects or increased side effects. One important tool to address this variability is therapeutic drug monitoring, which can be facilitated through simultaneous quantification of multiple oral anticancer drugs using liquid chromatography coupled to an Orbitrap mass spectrometer.
Article
Chemistry, Analytical
Nguyen Phuoc Long, Dayoung Heo, Hee-Yeon Kim, Tae Hyun Kim, Jae-Gook Shin, Anbok Lee, Dong-Hyun Kim
Summary: The study utilized a metabolomics-guided global pathway meta-analysis to investigate metabolic alterations in breast cancer, highlighting the importance of avoiding the loss of functionally relevant information and capturing key changes in the breast cancer metabolome. These alterations in metabolic pathways play crucial roles in breast cancer initiation, progression, metastasis, and drug resistance, providing insights for potential therapeutic targets.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemical Research Methods
Laura Colombaioni, Beatrice Campanella, Riccardo Nieri, Massimo Onor, Edoardo Benedetti, Emilia Bramanti
Summary: Mitochondria, organelles of bacterial origin, are not only responsible for energy production but also generate reactive oxygen species. They play a key role in cell defense regulation, cell stress, and damage. This study investigated the effect of hyperglycemia on the metabolome of hippocampal neurons and found that neuronal metabolism was impaired and typical fermentative compounds were produced.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Luisa Pozzo, Cristina Alcantara, Marta Selma-Royo, Izaskun Garcia-Mantrana, Emilia Bramanti, Vincenzo Longo, Maria Carmen Collado, Laura Pucci
Summary: This study evaluated the impact of the fermentation process on the chemical composition and SCCs levels of spelt from Garfagnana, as well as its effect on in vitro microbial growth and metabolism. The fermentation process altered the composition of spelt and modulated the intestinal microbiota, potentially promoting gut health in both normal weight and obese individuals.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Chemistry, Analytical
J. S. F. Pereira, R. Alvarez-Fernandez Garcia, M. Corte-Rodriguez, A. Manteca, J. Bettmer, K. L. LeBlanc, Z. Mester, M. Montes-Bayon
Summary: This study used SELTM-1 as a certified reference material to compare the selenium concentrations in intra- and extracellular regions using different experimental procedures. The sequential procedure involving cell washing and freeze drying provided the most accurate results for quantifying the selenium content in both regions.
Article
Chemistry, Multidisciplinary
Jan Labuda, Jiri Barek, Zuzana Gajdosechova, Heidi Goenaga-Infante, Linda J. Johnston, Zoltan Mester, Sergei Shtykov
Summary: Analytical chemistry is essential for understanding the behavior of engineered nanomaterials (ENMs). Various techniques are used for isolation, identification, and quantification of nanomaterials in different samples. Physicochemical characterization of ENMs is also important for assessing their properties. This report provides an overview of ENMs, their properties, and analytical scenarios. It also evaluates the current status of nanomaterial identification and characterization for regulatory purposes and presents an overview of nanomaterial metrology and available reference materials.
PURE AND APPLIED CHEMISTRY
(2023)
Article
Food Science & Technology
Teresa Grande, Aymen Souid, Marco Ciardi, Clara Maria Della Croce, Stefania Frassinetti, Emilia Bramanti, Vincenzo Longo, Luisa Pozzo
Summary: This study investigated the phenolic compounds, antioxidant activities, antimicrobial activity, and effect on Lactobacillus brevis growth of eight whole flours obtained from four ancient and modern wheat genotypes of Italian Triticum genus. The results showed quantitative differences in antioxidant activities, total phenolic content, and concentrations of five phenolic acids among the wheat genotypes. Wheat extracts had significant inhibitory effects on pathogenic bacteria and promoted the growth of L. brevis. The phenolic profile and antioxidant activities were influenced by the genotypic characteristics of the studied varieties, with the ancient Saragolla wheat standing out for its interesting phenolic profile.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Agricultural Engineering
Jose Gonzalez-Rivera, Beatrice Campanella, Elena Pulidori, Emilia Bramanti, Maria Rosaria Tine, Luca Bernazzani, Massimo Onor, Paolo Barberi, Celia Duce, Carlo Ferrari
Summary: Novel green extraction method (US-IMWAE) using simultaneous ultrasound and microwave irradiation allows for full exploitation of aromatic herbs while minimizing energy consumption and extraction cost. This method has successfully yielded essential oils with appealing composition profiles for cosmetic and medical industries, as well as valuable solid residues for alternative fuel production.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Chemistry, Physical
Elena Pulidori, Simone Micalizzi, Nikos Koutsomarkos, Emilia Bramanti, Maria Rosaria Tine, Giovanni Vozzi, Carmelo De Maria, Maria Chatzinikolaidou, Celia Duce
Summary: This study investigates the structure and correlations between structure, mechanical, and biological properties of gelatin-based materials. Gelatin electro-spun materials have higher content of ordered structure compared to gelatin casted films, which may be attributed to the random coil - alpha-helix transition during electrospinning. The presence of cross linker (GPTMS) decreases the ordered structures in gelatin casted films, while it does not affect the percentage of ordered structure in electrospun samples. The presence of keratin in gelatin/keratin electrospun biomaterials decreases alpha-helix content and promotes the conversion from antiparallel to parallel beta-sheet.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Chemistry, Analytical
Enea Pagliano, Elizabeth Keegan, Zsolt Varga, Kenny Nadeau, Juris Meija, Henri Wong, Christopher Vardanega, Maria Wallenius, Klaus Mayer, Chris Cochrane, Ali El-Jaby, Zoltan Mester
Summary: This study quantified the inorganic anions in three uranium ore concentrates, which is useful for nuclear forensics to establish sample provenance.
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Ophelie Ranquet, Celia Duce, Emilia Bramanti, Patrick Dietemann, Ilaria Bonaduce, Norbert Willenbacher
Summary: The authors use a combination of egg yolk and two pigments to investigate how different distribution of proteinaceous binder can control the flow behavior, drying kinetics, and chemistry of oil paints. This study demonstrates that adjusting the distribution of proteinaceous binder can enhance the stiffening effect and prevent undesired absorption of humidity, improving the brushability and preserving invaluable artworks.
NATURE COMMUNICATIONS
(2023)
Article
Medicine, General & Internal
Edoardo Benedetti, Claudia Barate, Fabrizio Mavilia, Emilia Bramanti, Riccardo Morganti, Valentina Guerri, Giulia Cervetti, Enrico Capochiani, Ilaria Bertaggia, Salvatore Massimo Stella, Ginevra Traverso, Benedetto Bruno, Sara Galimberti
Summary: Fixed duration VenR treatment showed significant improvements in PFS and uMRD compared to bendamustine-rituximab in relapsed/refractory CLL patients. Ultrasonography and palpation were suggested as possible techniques to evaluate visceral and superficial lymph node involvement outside of clinical trials.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Beatrice Campanella, Stefano Legnaioli, Massimo Onor, Edoardo Benedetti, Emilia Bramanti
Summary: Saliva is a sample that can be easily collected and contains a wealth of biochemical information related to an individual's health status. The fast and straightforward analysis of saliva using vibrational spectroscopy provides a good basis for large-scale preclinical studies. This study explored the effects of saliva collection (spitting/swab) and processing (deproteinization procedures) and found that special care is needed when using swabs due to the significant interaction between the polymeric material and saliva components. Additionally, analyzing saliva before and after deproteinization by FTIR and Raman spectroscopy provides complementary biological information.
Article
Chemistry, Analytical
Emanuela Pitzalis, Beatrice Campanella, Rachele Bonini, Massimo Onor, Alessandro D'Ulivo
Summary: The mechanisms controlling the generation of PbH4 in the reaction of Pb(II) with NaBH4 were investigated, with and without the presence of K3Fe(CN)6. PbH4 was identified for the first time using gas chromatographic mass spectrometry. It was found that without the additive, Pb(II) is converted to solid species and no volatile lead species can be detected. In the presence of K3Fe(CN)6, PbH4 is formed by the direct transfer of hydride from borane to lead atoms.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Kelly L. LeBlanc, Gregoire Horndli, Marc-Antoine Bergeron, Zhigen Zhang, Patrick Denoncourt, Zoltan Mester
Summary: Selenized yeast is widely used as a source of selenium in dietary supplements and research due to its high bioavailability and formation of selenium-containing metabolites. Accurate quantitation of selenomethionine, the major form of selenium in selenized yeasts, is challenging and time-consuming. The National Research Council Canada has developed an 82-Se-enriched selenized yeast Certified Reference Material, SEEY-1, which can be used as a matrix-matched spike for the analysis of selenomethionine. SEEY-1 enables reliable analysis of Se amino acid content in under an hour compared to the conventional hydrolysis method.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Michelle Buoso, Beatrice Campanella, Massimo Onor, Emanuela Pitzalis, Alessandro D'Ulivo
Summary: The use of BH intermediates as derivatization reagents significantly improves the tolerance to interference from transition metal ions in the generation of SbH3 and BiH3, and shows high tolerance in a wide range of acidity. The method has been successfully applied to the determination of Sb and Bi in complex samples, providing further understanding of the chemical behavior of borohydrides.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2024)
Article
Biochemical Research Methods
Kelly L. LeBlanc, Tantima Kumlung, Andres Suarez Priede, Paramee Kumkrong, Thippaya Junvee, Suladda Deawtong, Jorg Bettmer, Maria Montes-Bayon, Zoltan Mester
Summary: Mushrooms are considered a valuable food source due to their high protein and fiber and low fat content. This study showed that selenium-enriched Lingzhi mushrooms contained low levels of selenium, with selenomethionine being the most abundant form. The mycelium of these mushrooms showed significant selenium accumulation and the presence of selenium nanoparticles, which could have potential medicinal value.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
