Article
Multidisciplinary Sciences
Xiaoli Lei, Junli Wang, Zhigang Yang
Summary: This study aimed to develop and externally validate a novel prediction model using the T-SPOT.TB test to assess the probability of TPE in patients with unexplained pleural effusion. The developed model showed good discrimination and calibration, with a sensitivity and specificity of 70% and 88% in the derivation population and 77% and 92% in the validation population, respectively. This predictive model provides a useful tool for identifying TPE in clinical practice.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Analytical
Kaiwen Li, Zhuo Shi, Yuwei Wang, Feng Yan, Zhanhong Li, Zifeng Wang, Zhigang Zhu
Summary: This study developed a label-free electrochemical aptasensor using Ti3C2Tx-Ag/Au NPs for CRP detection. The sensor showed a dual-amplified signal strategy and excellent detection performance, with great potential for clinical diagnostic applications.
MICROCHEMICAL JOURNAL
(2023)
Article
Biophysics
Jian-Hong Zhu, Li-Ping Mei, Ai-Jun Wang, Yan-Yan Song, Jiu-Ju Feng
Summary: A split-typed PEC aptasensor using phosphate-functionalized Pt/TiO2 material was developed for the detection of low-abundance aden-osine deaminase (ADA) activity. The biosensor showed a broad linear range and a low limit of detection, filling a gap in the analysis of ADA activity. This research provides valuable insights for ADA-related research and clinical diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Yuan Zhao, Jie Yao, Zhenglong Wu, Han Liu, Wangwang Zheng
Summary: The strong and stable electrochemical beacons are crucial for sensitive and reliable electroanalysis applications. AuPt nanoparticles showed enhanced electrochemical oxidation performance under light illumination, making them suitable for detecting EpCAM with a ratiometric electrochemical aptasensor design that achieved sensitive and reliable quantification in a specific range. This study suggests the possibility of designing more robust and anti-interference electroactive plasmonic metal-Pt composite nanostructures for future electroanalysis applications.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Alberto Monroy-Mora, Maria de Lourdes Mora-Garcia, Katia Alheli Monroy Mora, Jorge Hernandez-Montes, Rosario Garcia-Rocha, Christian Azucena Don-Lopez, Benny Weiss-Steider, Juan Jose Montesinos-Montesinos, Alberto Monroy-Garcia
Summary: The presence and activity of ADA in cervical cancer tumor cells can provide protection against the cytotoxic effect of high ADO contents in the tumor microenvironment. Therefore, the inhibition of ADA could be a strategy for the treatment of cervical cancer.
Article
Multidisciplinary Sciences
Jianan Li, Wenxia Yu, Shisheng Huang, Susu Wu, Liping Li, Jiankui Zhou, Yu Cao, Xingxu Huang, Yunbo Qiao
Summary: Both ABEs and CBEs can induce transcriptome-wide RNA off-target editing independent of gRNA. Engineering ABEmax variants has successfully achieved minimized RNA off-target activities as the goal.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Shunlin Yu, Chuan Liu, Songjia Han
Summary: In this study, a unique and simple method for preparing nanoporous gold (NPG) was reported, with the structure of the NPG-based electrode further controlled through post-treatment processes. The resulting NPG electrodes were demonstrated to have higher sensitivity in flexible photodetectors compared to common planar photodetectors. This work opens up possibilities for the future application of flexible and low-cost nanoporous metals in electronics.
Article
Cell Biology
Yanyan Ling, Chuan Jiang, Zhenzhen Xiao, Xiao Shang, Qi Li, Baojie Wang, Maolin Hao, Fei Liu, Nannan Zhao, Jianli Feng, Hongqin Zhao
Summary: This study found that serum ADA activity is significantly decreased in patients with acute cerebral infarction (ACI), indicating that decreased ADA concentrations may be involved in the pathogenesis of ACI. Smoking and alcohol consumption further decrease ADA concentrations in ACI patients, while diabetes and hypertension have the opposite effect.
Article
Biochemical Research Methods
Moeko Ito, Hiroshi Nihira, Kazushi Izawa, Takahiro Yasumi, Ryuta Nishikomori, Sachiko Iwaki-Egawa
Summary: The new DBS ADA2 activity assay is experimentally simple, highly adaptable, and requires no special equipment except for a microplate reader. Low background noise was achieved with heparin-affinity purification. The method effectively differentiates between healthy controls and patients, reliably measuring ADA2 activity in DBS and providing an opportunity for early diagnosis of DADA2.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Chemistry, Analytical
Lei Shang, Bing-Jiao Shi, Wei Zhang, Li-Ping Jia, Rong-Na Ma, Qing-Wang Xue, Huai-Sheng Wang, Wei Yan
Summary: Aptasensor based on electrochemical stripping chemiluminescence technology combined with DNA nanoclusters shows high sensitivity and good performance in detecting CEA.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Alan Meng, Xiaocheng Hong, Yue Zhang, Jifang Yin, Liying Sheng, Zhenjiang Li
Summary: The electrochemical aptasensor based on L-Glutamic acid and peptides is sensitive, antifouling, and effective in detecting ATP. It shows a wide linear range, low detection limit, excellent selectivity, reproducibility, and stability. The sensor also demonstrates consistent performance in different solutions, indicating its potential for clinical analysis.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Miaoran Xu, Hao Dang, Mengfei Wang, Shuai Zhang, Qiaojuan Jia, Jia-Yue Tian, Minghua Wang, Miao Du
Summary: In this study, a new dual-modal electrochemical (EC)-photoelectrochemical (PEC) aptasensing strategy has been developed for the sensitive determination of penicillin G (PG) in complex environments. An Fe-doped covalent triazine framework (Fe-MA-Dha-CTF) was synthesized and demonstrated enhanced photoelectric conversion efficiency, superior electrochemical activity, and high bioaffinity towards the PG-targeted aptamer. The established Fe-MA-Dha-CTF-based dual-modal EC-PEC aptasensor exhibited ultralow limits of detection and high selectivity, stability, reproducibility, and practicability in diverse real samples. This CTF-based dual-modal aptasensing approach extends the application of porous-organic frameworks in biosensing.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Agata Jedrzejewska, Ada Kawecka, Alicja Braczko, Marzena Romanowska-Kocejko, Klaudia Stawarska, Milena Deptula, Malgorzata Zawrzykraj, Marika Franczak, Oliwia Krol, Gabriela Harasim, Iga Walczak, Michal Pikula, Marcin Hellmann, Barbara Kutryb-Zajac
Summary: This study investigated the activities of circulating ADA isoenzymes in patients 8 weeks after mild COVID-19 and related them to inflammation and microvascular/endothelial function parameters. The study found that post-COVID patients had microvascular dysfunction associated with changes in endothelial dysfunction and inflammatory activation parameters. Additionally, the circulating activities of ADA1 and ADA2 were diminished in post-COVID patients, and this study suggests a possible role of ADA isoenzymes in cardiovascular complications after mild COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Xin Zhang, Jie Cui, Junling Hou, Wenquan Wang
Summary: This review discusses the pharmacological effects of natural active substances with uric-acid-reducing activity. These substances, including natural extracts, plant monomer compounds, natural protease hydrolysates, peptides, and probiotic bacteria, have been shown to be effective in the treatment of hyperuricemia with few side effects. The mechanisms by which these substances exhibit hypouricemic effects include inhibition of key enzyme activities, promotion of uric acid excretion and inhibition of reabsorption in the kidney, promotion of decomposing uric acid precursors, and promotion of decomposing uric acid. Understanding and harnessing the potential of these natural active substances is of great importance for the treatment of hyperuricemia.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Chemistry, Analytical
Yong Chang, Yanyan Wang, Jingyi Zhang, Yuejiao Xing, Gang Li, Dehua Deng, Lin Liu
Summary: Magnetically assisted biosensors have important applications in electrochemical biosensors, enabling separation and pre-concentration of targets through the combination of magnetic materials and biomolecules, and obtaining signals through measurements such as redox current.
Article
Biophysics
Kai Zhang, Zhenqiang Fan, Bo Yao, Yuedi Ding, Jing Zhao, Minhao Xie, Jianbin Pan
Summary: Siglec-5 is an inhibitory receptor for leukocytes in the human body, and the development of novel assays for Siglec-5 can aid in studying related disease pathogenesis and developing therapeutic drugs. A biosensor based on CRISPR-Cas12a and MXene has been developed for sensitive detection of Siglec-5, extending the application of CRISPR-Cas12a to biosensor construction and providing a common method for biomarker detection. This work represents the first investigation into the Trans-Cleavage activity of CRISPR-Cas12a for Mxene-based ECL biosensor establishment.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Kai Zhang, Zhenqiang Fan, Bo Yao, Tingting Zhang, Yuedi Ding, Sha Zhu, Minhao Xie
Summary: In this study, an entropy-driven electrochemiluminescence (ECL) biosensor was constructed for ultra sensitive bioassay of NF-kappa B p50, providing a repeatable approach for TFs detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Kai Zhang, Lei Zhou, Tingting Zhang, Zhenqiang Fan, Minhao Xie, Yuedi Ding, Hao Li
Summary: A biosensing method was developed to detect protein function under pathological conditions, showing oxidative stress-induced protein translocation and modification. Clinical samples confirmed the effectiveness of this method in studying the role of proteins in the progression of colorectal cancer.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Nanoscience & Nanotechnology
Bo Yao, Jing Zhang, Zhenqiang Fan, Yuedi Ding, Bin Zhou, Runlin Yang, Jianfeng Zhao, Kai Zhang
Summary: A novel Au@Ti3C2@PEI-Ru(dcbpy)(3)(2+) nanocomposite-based ECL biosensor was designed for the sensitive detection of the SARS-CoV-2 RdRp gene. The sensor showed a wide detection range and a low limit of detection, indicating its great potential for clinical medical applications. The use of a DNA walker amplification strategy also showed promising results for other detection methods.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biophysics
Zhenqiang Fan, Bo Yao, Yuedi Ding, Jing Zhao, Minhao Xie, Kai Zhang
Summary: A novel sensor for SARS-CoV-2 detection has been established utilizing an entropy-driven amplified electrochemiluminescence strategy, based on DNA tetrahedron design. By avoiding expensive enzyme reagents, the sensor efficiently detects viral RNA with high specificity and sensitivity, providing a reliable platform for large-scale screening of COVID-19 patients.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Yuedi Ding, Jun Fan, Zhenqiang Fan, Kai Zhang
Summary: The study demonstrated that ?-tocotrienol reversed the MDR of MCF-7/Adr cells by inhibiting the expression and function of mdr1/P-gp, as well as reducing the activation of the NF-?B signaling pathway.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Analytical
Zhenqiang Fan, Bo Yao, Yuedi Ding, Minhao Xie, Jianfeng Zhao, Kai Zhang, Wei Huang
Summary: A novel aptasensor was developed utilizing high affinity aptamers and non-toxic nanocomposites for the bioassays of Siglec-5. The sensor showed a low detection limit and significant potential for practical applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Yuedi Ding, Zhenqiang Fan, Bo Yao, Dong Xu, Minhao Xie, Kai Zhang
Summary: A novel nanoparticle-based fluorescence probe was developed for NF-kappa B transcription factor detection and in situ imaging. The probe utilizes steric hindrance to quench and restore fluorescence signals, achieving a low detection limit and a good linear range.
Article
Biophysics
Kai Zhang, Zhenqiang Fan, Yuedi Ding, Jinlong Li, Hao Li
Summary: A low-cost and simple operation virus protein serum assay has been proposed, targeting the main protease of the virus through a short probe mimicking its substrate. Successful detection of the virus marker protein in the serum of infected patients encourages further development of this method for clinical practice.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Engineering, Environmental
Kai Zhang, Zhenqiang Fan, Yuedi Ding, Minhao Xie
Summary: This study successfully constructed a method for SARS-CoV-2 nucleic acid detection using an exonuclease III cleavage reaction-based isothermal amplification combined with a pH-induced regenerative ECL biosensor mediated by CRISPR/Cas12a, providing ultra-sensitive and specific detection capabilities essential for large-scale screening in resource-limited areas.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Zhenqiang Fan, Bo Yao, Yuedi Ding, Dong Xu, Jianfeng Zhao, Kai Zhang
Summary: The study developed a dual-wavelength ratiometric electrochemiluminescence (ECL) biosensor based on entropy-driven and DNA walker cycle amplification strategies for detecting the RNA-dependent RNA polymerase (RdRp) gene of SARS-CoV-2. This method achieved high sensitivity detection of the SARS-CoV-2 RdRp gene, making it suitable for early clinical diagnosis.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Analytical
Kai Zhang, Zhenqiang Fan, Yue Huang, Yuedi Ding, Minhao Xie
Summary: Early diagnosis and timely management of SARS-CoV-2 are essential for controlling the epidemic. This study developed a novel ECL biosensor for detecting the RdRp gene of SARS-CoV-2, utilizing CRISPR-Cas12a and a 3D DNA walker for amplification. The system showed a high sensitivity with a detection limit of 12.8 aM, demonstrating the potential for rapid and convenient detection of SARS-CoV-2-associated nucleic acids.
Article
Chemistry, Analytical
Yuxin Lin, Beibei Nie, Xinyu Qu, Minghui Wang, Jie Yang, Genxi Li
Summary: Covalent organic frameworks (COFs) have attracted much attention for their unique physical-chemical characteristics and potential applications in biosensing. In this study, spherical COFs with regular shape and good dispersion were synthesized, and a novel nanoprobe was developed by modifying Histostar on the COFs surface. The nanoprobe was then applied to fabricate an electrochemical biosensor for exosomes detection, achieving a low detection limit and wide linear detection range. This work offers a promising platform for ultrasensitive detection of exosomes.
Article
Chemistry, Multidisciplinary
Kai Zhang, Jinlong Li, Zhenqiang Fan, Hao Li, Jing-Juan Xu
Summary: A new protocol has been established for the detection of SARS-CoV-2 using a peptidomimetic to covalently detect a viral marker protease.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Zhenqiang Fan, Yuedi Ding, Bo Yao, Jiaying Wang, Kai Zhang
Summary: In this study, we successfully detected NF-kappa B p50 by exploiting the double-stranded DNA binding property of transcription factor and the trans cleavage characteristic of CRISPR-Cas12a.
CHEMICAL COMMUNICATIONS
(2021)