Article
Automation & Control Systems
Sofie B. Knudsen, Jan H. Christensen, Giorgio Tomasi
Summary: Separating nitrogen containing heterocyclic aromatic compounds in oil products is challenging due to the high number of structural isomers. Ionmobility spectrometry (IMS) can address this problem by separating molecules according to their bulkiness, and when combined with mass spectrometry on multiple samples, three-way arrays can be generated to enhance resolution. However, resolving these isomers in chromatography, even with MS, is difficult because they have similar volatility, polarity, and mass spectra.
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
(2021)
Article
Biochemical Research Methods
Francis Berthias, Berwyck L. J. Poad, Hayden A. Thurman, Andrew P. Bowman, Stephen J. Blanksby, Alexandre A. Shvartsburg
Summary: The prevalence and functional importance of isomeric biomolecules in biochemistry is a hot topic, but identifying and distinguishing them can be challenging due to identical mass. Modern mass spectrometry offers compositional assignments, but proteomics and lipidomics are still facing difficulties in deciphering functional networks due to lack of isomeric depth. The introduction of a new platform utilizing high-resolution differential ion mobility spectrometry (FAIMS) and ozone-induced dissociation (OzID) provides a solution for identifying isomeric lipids. Metal cationization improves FAIMS resolution, but the yield diminishes for certain metals like Cu+. Argentination strikes a balance between separation efficiency and diagnostic fragmentation for optimal FAIMS/OzID performance.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
A. Bianco, I Neefjes, D. Alfaouri, H. Vehkamaki, T. Kurten, L. Ahonen, M. Passananti, J. Kangasluoma
Summary: Mass spectrometry is an effective method for identifying and quantifying environmentally relevant molecules, but it cannot distinguish between isomers. This study combines an experimental set-up using a differential mobility analyser with computational ion mobility calculations to successfully identify isomers. The experimental set-up allows for analysis in both the gas and liquid phase, making it a versatile tool for isomer separation.
Article
Chemistry, Analytical
Irina Oganesyan, Joanna Hajduk, Julian A. Harrison, Adrien Marchand, Martin F. Czar, Renato Zenobi
Summary: This study characterizes the A2 N-glycans and their conformers using ion mobility spectroscopy, hydrogen/deuterium exchange, and electron capture dissociation. It identifies multiple gas-phase structures for each N-glycan, and successfully distinguishes and identifies each isomer using collision cross section and fragmentation analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Soumya Mukherjee, John C. Fjeldsted, Colin L. Masters, Blaine R. Roberts
Summary: The study successfully utilized a new high-resolution demultiplexing technique to improve the resolution of amyloid beta in the brains of Alzheimer's disease patients, revealing the role of its isomers in plaque formation.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Nour Mashmoushi, Daniel R. Juhasz, Neville J. A. Coughlan, Bradley B. Schneider, J. C. Yves Le Blanc, Mircea Guna, Blake E. Ziegler, J. Larry Campbell, W. Scott Hopkins
Summary: Two ion populations of protonated Rivaroxaban are separated using differential mobility spectrometry under different conditions, showing similarities in CID behavior but distinct prototropic isomers based on UVPD spectra. The populations are attributed to bare protonated Rivaroxaban and to complexes with ammonia or acetonitrile, which dissociate prior to mass selection. Comparisons with computed spectra suggest proton sharing between distal oxygen centers in the identified prototropic isomers.
JOURNAL OF PHYSICAL CHEMISTRY A
(2021)
Article
Chemistry, Analytical
Aurelie Delvaux, Estelle Rathahao-Paris, Blanche Guillon, Sophie Cholet, Karine Adel-Patient, Francois Fenaille, Christophe Junot, Sandra Alves
Summary: The study introduces a high throughput and high resolution method for characterizing main oligosaccharides in breastmilk using ion mobility spectrometry, offering high identification level. Standard HMO isomers were investigated and could be distinguished effectively. Multiple mobility peaks were observed for some HMO species, but reduction at the reducing-end did not significantly improve isomer distinction.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Chengyi Xie, Lei Li, Qidi Wu, Pengfei Guan, Chenlu Wang, JianCheng Yu, Keqi Tang
Summary: The study systematically investigated the effect of alkaline earth cations and halogen anions on the separation of isomeric carbohydrates, finding that the [M + Ca + I]+ complex was the optimum complex for enhancing IMS separation resolution. This complex showed effective separation capability for all 21 pairs of isomeric oligosaccharides used in the study and could potentially be used as an effective complex for enhancing IMS separation resolution of isomeric carbohydrates.
Review
Biochemistry & Molecular Biology
Sandra M. Camunas-Alberca, Maria Moran-Garrido, Jorge Saiz, Alberto Gil-de-la-Fuente, Coral Barbas, Ana Gradillas
Summary: It is important to conduct a detailed molecular structure analysis of isomeric lipids to understand their biological roles better. Conventional tandem mass spectrometry (MS/MS) is complicated by isomeric interference, necessitating the development of specialized methodologies to separate lipid isomers. This review discusses recent lipidomic studies using ion mobility spectrometry combined with mass spectrometry (IMS-MS) and describes examples of separating and elucidating the structural and stereoisomers of lipids based on their ion mobility behavior.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Multidisciplinary Sciences
Xiaoyu Zhou, Zhuofan Wang, Jingjin Fan, Zheng Ouyang
Summary: Ion mobility is utilized in mass spectrometers for analyzing the structure of biomolecules. Researchers demonstrate that ion mobility analysis in an ion trap with ultra-high fields can achieve resolutions over 10,000 for isomer separation, as shown for disaccharide, phospholipid, and peptide isomers. Elucidating complex structures of biomolecules is crucial in chemistry and life sciences. In the last decade, ion mobility coupled with mass spectrometry has become a unique tool for distinguishing isomers and isoforms of biomolecules. This study presents a concept for ion mobility analysis using an ion trap, enabling ultra-high field isomer separation and achieving resolutions over 10,000. The potential of this technology is demonstrated for analyzing isomers of disaccharides, phospholipids, and peptides with post-translational modifications.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Katerina V. Djambazova, Martin Dufresne, Lukasz G. Migas, Angela R. S. Kruse, Raf Van de Plas, Richard M. Caprioli, Jeffrey M. Spraggins
Summary: Gangliosides are diverse acidic glycosphingolipids with distinct biological roles. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) can be used for spatial analysis of gangliosides directly from tissue sections. The integration of trapped ion mobility spectrometry with MALDI IMS enables the analysis of isomeric lipid structures in situ. Gas-phase separation and identification of disialoganglioside isomers GD1a and GD1b, with different sialic acid residue positions, were demonstrated in multiple samples, including tissue sections.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Yang Li, Yujiao Qin, Songchang Wei, Ling Ling, Chuan-Fan Ding
Summary: Steroids are important indicators of health and disease. Differentiating between steroids, especially their isomers, can be challenging due to their structural similarities. This study presents a trapped ion mobility spectrometry-mass spectrometry (TIMS-MS) approach based on steroid analogue adduction for specific identification of isomers. The use of dexamethasone (DEX) as a heterodimer enhancer allowed successful separation of isomer pairs in TIMS.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Ralph Aderorho, Christopher D. Chouinard
Summary: Fentanyl, a potent opioid, has raised concerns due to its illegal production and distribution, leading to misuse, overdose, and deaths. This study aimed to develop a rapid method for detecting and separating fentanyl isomers using ion mobility-mass spectrometry (IM-MS). Several strategies for improved differentiation were implemented, resulting in confident detection and subsequent quantitative analysis.
DRUG TESTING AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Pengfei Guan, Chengyi Xie, Lei Li, Xiangyu Fang, Fangling Wu, Jun Jack Hu, Keqi Tang
Summary: Carbohydrates are crucial in early life evolution, with their structures determining their roles in biological systems. Ion mobility spectrometry is seen as a promising tool for carbohydrate structure elucidation. Analysis of disaccharides using trapped ion mobility spectrometry-mass spectrometry in the negative ion mode showed that using negative charge states, specifically complexing with iodide anions, can improve differentiation of isomeric disaccharides.
Article
Biochemistry & Molecular Biology
Wojciech Fabianowski, Miroslaw Maziejuk, Monika Szyposzynska, Monika Wisnik-Sawka
Summary: The article presents a technique of differential ion mobility spectrometry (DMS) for the detection and identification of volatile organic compounds (VOCs) from various categories. It analyzes the possibility of mixture identification using only the DMS spectrometer and provides several examples. The article also discusses the analysis of different compounds, their mechanisms of fragmentation, influence on effective ion temperature, and high electric field intensity.
Article
Biochemical Research Methods
Chia-Feng Tsai, Rui Zhao, Sarah M. Williams, Ronald J. Moore, Kendall Schultz, William B. Chrisler, Ljiljana Pasa-Tolic, Karin D. Rodland, Richard D. Smith, Tujin Shi, Ying Zhu, Tao Liu
MOLECULAR & CELLULAR PROTEOMICS
(2020)
Article
Chemistry, Analytical
Emma K. Sisley, Jakub Ujma, Martin Palmer, Kevin Giles, Francisco A. Fernandez-Lima, Helen J. Cooper
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Oliver J. Hale, Helen J. Cooper
Summary: By utilizing nanospray-desorption electrospray ionization (nano-DESI), native mass spectrometry imaging (native MSI) has achieved significant improvements in resolution compared to native LESA MSI, allowing for more precise imaging. This technique can also be used for location-targeted proteomic analysis directly from tissues.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Jana Havlikova, Robin C. May, Iain B. Styles, Helen J. Cooper
Summary: The ESKAPE pathogens were successfully identified using liquid extraction surface analysis mass spectrometry (LESA MS), with a identification success rate of 79%.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Physics, Atomic, Molecular & Chemical
Oliver J. Hale, James W. Hughes, Helen J. Cooper
Summary: In this study, the integration of travelling wave ion mobility spectrometry (TWIMS) into native nanoDESI MSI workflow was demonstrated, showing improved specificity in ion images by allowing subtraction of chemical noise and enabling the calculation of collision cross sections for protein structure. Results presented ion images and collision cross sections for a variety of proteins and protein assemblies with molecular weights up to 42.6 kDa, showing good agreement with experimental and literature-derived values.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Chemistry, Multidisciplinary
Emma K. Sisley, Oliver J. Hale, Iain B. Styles, Helen J. Cooper
Summary: This study demonstrates the use of mass spectrometry imaging to map the noncovalent interactions of proteins in the tissue environment of rat brain. By directly identifying proteins from tissue using nanospray-desorption electrospray ionization, the spatial distributions of ligand-bound and metal-bound proteins were mapped. Additionally, the nature of the ligand was confirmed through multiple rounds of tandem mass spectrometry. The study also identified several natively monomeric proteins displaying distinct spatial distributions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Editorial Material
Biochemical Research Methods
Helen J. Cooper, Aneika C. Leney
Article
Chemistry, Analytical
Oliver J. Hale, James W. Hughes, Emma K. Sisley, Helen J. Cooper
Summary: This study demonstrates the detection and identification of a range of intact endogenous protein assemblies with various stoichiometries from different tissue types using multiple NAMS techniques, showing a greater than twofold increase in accessible molecular weight. Additionally, spatial distributions of protein assemblies up to 94 kDa were mapped in brain and kidney by nanospray desorption electrospray ionization (nano-DESI) mass spectrometry imaging.
ANALYTICAL CHEMISTRY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Melissa M. Grant, John J. Taylor, Katrin Jaedicke, Andrew Creese, Catherine Gowland, Bernard Burke, Khawla Doudin, Upen Patel, Paul Weston, Michael Milward, Susan M. Bissett, Helen J. Cooper, Gerben Kooijman, Amir Rmaile, Marko de Jager, Philip M. Preshaw, Iain L. C. Chapple
Summary: Differential protein biomarkers in saliva and gingival crevicular fluid can be used to objectively distinguish between periodontal health and disease states.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Eva Illes-Toth, Oliver J. Hale, James W. Hughes, Nicole Strittmatter, Jonathan Rose, Ben Clayton, Rebecca Sargeant, Stewart Jones, Andreas Dannhorn, Richard J. A. Goodwin, Helen J. Cooper
Summary: This study demonstrates for the first time the in situ mass spectrometry analysis of a non-covalent protein-drug complex formed in vivo, and provides a direct route for target-drug characterization in early stage drug discovery by spatial mapping across liver tissue.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Oliver J. Hale, Helen J. Cooper
Summary: This study demonstrates a mass spectrometry analysis method for the direct analysis of intact oligomeric membrane proteins from tissue. By characterizing protein assembly directly from sheep eye lens sections without sample pre-treatment, our approach allows for simultaneous analysis of soluble protein assemblies.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemical Research Methods
Eva Illes-Toth, Christopher J. Stubbs, Emma K. Sisley, Jeddidiah Bellamy-Carter, Anna L. Simmonds, Todd H. Mize, Iain B. Styles, Richard J. A. Goodwin, Helen J. Cooper
Summary: Liquid extraction surface analysis coupled with native mass spectrometry was used to quantitatively analyze protein-ligand interactions. The results showed good consistency when both protein and ligand were present in the sample.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Chemistry, Multidisciplinary
Emma K. Sisley, Oliver J. Hale, James W. Hughes, Helen J. Cooper
Summary: Native ambient mass spectrometry allows for in situ analysis of proteins in tissue, providing both structural and spatial information. This study demonstrates the analysis of membrane proteins using this technique, including integral membrane proteins and membrane-associated proteins. The results show that a washing protocol can effectively remove soluble proteins without disrupting the spatial distribution of the membrane proteins.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Yuying Du, Robin C. May, Helen J. Cooper
Summary: In this study, we demonstrated that intact non-covalent protein complexes can be directly detected from bacterial colonies growing on agar by combining electroporation with native ambient mass spectrometry. Homodimers HdeA and HdeB, as well as the 50 kDa Mn-bound superoxide dismutase homodimer, were identified, along with some previously undetected monomeric proteins.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Christopher R. Conant, Isaac K. Attah, Sandilya V. B. Garimella, Gabe Nagy, Aivett Bilbao, Richard D. Smith, Yehia M. Ibrahim
Summary: In this study, five different waveforms were characterized in terms of their transmission efficiency, IMS resolution, and resolving power, with triangle and asymmetric ramp profiles found to offer modestly greater resolution and resolving power compared to others. The research also revealed that separations were generally improved with higher TW amplitudes, moderately improved by lower speeds, but experienced decreases in signal intensity under extreme operating conditions.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
