Article
Genetics & Heredity
Pooja Agrawal, Shanmukh Katragadda, Arun K. Hariharan, Vijayashree Gauribidanur Raghavendrachar, Arunika Agarwal, Rashmi Dayalu, Disha Awasthy, Sanjay C. Sharma, Yasodha Kannan Sivasamy, P. Lakshmana, Ashwini Shanmugam, Vamsi Veeramachaneni, Vaijayanti Gupta, B. P. Vani, Lekha Subaiya, T. S. Syamala, Ramesh Hariharan, Vijay Chandru, David E. Bloom
Summary: DBS samples show similar performance to venous blood samples in whole-genome sequencing, making them a feasible alternative for studies requiring WGS analysis. Various downstream analyses, including mitochondrial heteroplasmy detection and determination of telomere lengths, also showed comparable results between DBS and venous blood samples.
BMC MEDICAL GENOMICS
(2021)
Article
Genetics & Heredity
Yan Ding, Mallory Owen, Jennie Le, Sergey Batalov, Kevin Chau, Yong Hyun Kwon, Lucita van der Kraan, Zaira Bezares-Orin, Zhanyang Zhu, Narayanan Veeraraghavan, Shareef Nahas, Matthew Bainbridge, Joe Gleeson, Rebecca J. Baer, Gretchen Bandoli, Christina Chambers, Stephen F. Kingsmore
Summary: Universal newborn screening (NBS) is a successful public health intervention, and archived dried bloodspots (DBS) collected for NBS can provide high-quality genomic DNA (gDNA) for population genomic studies without the need for PCR amplification. This pilot study demonstrates simple methods for gDNA extraction and whole genome sequencing (WGS) library preparation from DBS up to 20 years old, yielding WGS of sufficient quality and quantity for variant calling.
NPJ GENOMIC MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Antonella Romano, Candida Zuchegna, Giuseppa Zannini, Samantha Messina, Clorinda Fusco, Antonio Pezone, Roberta Grillo, Paola de Candia, Giuseppe Matarese, Antonio Porcellini
Summary: This study developed a new method for extracting genomic DNA from dried blood spots (DBS) and evaluated its performance in genotyping mouse models of obesity.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Environmental Sciences
Jeremy P. Koelmel, Elizabeth Z. Lin, Emily Parry, Paul Stelben, Emma E. Rennie, Krystal J. Godri Pollitt
Summary: Non-targeted PFAS analysis was applied to human blood to understand the sources, concentrations, and toxicity of these compounds. High-resolution tandem mass spectrometry and software workflow were used for PFAS characterization in dried blood spots. A total of 28 PFAS and 86 potential PFAS were detected, providing insights for environmental epidemiology studies and policy-making.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Parasitology
Emily L. Pascoe, Ankje de Vries, Helen J. Esser, Constantianus J. M. Koenraadt, Hein Sprong
Summary: In this study, it was demonstrated that tick-borne encephalitis virus (TBEV) RNA can be detected in non-destructive samples obtained from naturally infected wild rodents, providing a practical alternative for long-term surveillance of the virus.
PARASITES & VECTORS
(2023)
Article
Biochemical Research Methods
Anne-Marie Patenaude, Julija Erhardt, Rene Hennig, Erdmann Rapp, Gordan Lauc, Marija Pezer
Summary: The association of IgG glycosylation changes with human diseases and physiological conditions is well recognized, but the mechanistic explanation remains elusive. Mouse models are now being looked at for intervention studies to understand causality. A protocol for analyzing total N-glycans of IgG isolated from mouse dried blood spots has been developed, showing that mouse DBS are a good source for IgG N-glycan analysis.
Article
Chemistry, Analytical
Lucia Paniagua-Gonzalez, Elena Lendoiro, Esteban Otero-Anton, Manuel Lopez-Rivadulla, Ana de-Castro-Rios, Angelines Cruz
Summary: This study aimed to perform a clinical validation for tacrolimus and mycophenolic acid in both DBS and MitraTM VAMS, fulfilling both analytical and clinical acceptance criteria. VAMS showed lower concentrations compared to routine matrices but met the analytical requirements after correction.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Medical Laboratory Technology
Franklin Ducatez, Carine Pilon, Justine Ferey, Stephane Marret, Soumeya Bekri, Abdellah Tebani
Summary: This study evaluated the impact of different hematocrit levels and punching positions on the assessment of enzyme activity in dried blood spot (DBS) samples. The results showed that hematocrit, punching position, and the use of different sampling methods did not affect the assessment of enzyme activity, indicating the reliability of DBS in this test.
CLINICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Marc Luginbuhl, Stefan Gaugler
Summary: This perspective discusses quantitative DBS analysis for anti-doping testing in an athletic population and argues that using only volumetric sampling may not be sufficient. It provides examples of HCT variations, discusses the whole blood to plasma ratio and HCT extraction bias, and presents options to correct for the HCT bias.
DRUG TESTING AND ANALYSIS
(2021)
Article
Endocrinology & Metabolism
Jesper Krogh, Peter Plomgaard, Ruth Frikke-Schmidt, Sten Velschow, Jesper Johannesen, Linda Maria Hilsted, Malene Schroder, Ulla Feldt-Rasmussen
Summary: This study assessed the performance and safety of Fluispotter for the first time in healthy subjects. Fluispotter is a novel body-worn device with automated extraction, collection, and storage of venous dried blood spot samples. The majority of participants were able to successfully collect all the samples, demonstrating that Fluispotter is safe and feasible.
ENDOCRINE CONNECTIONS
(2023)
Article
Chemistry, Analytical
Ethel D. Weld, Teresa L. Parsons, Ryann Gollings, Marybeth McCauley, Beatriz Grinsztejn, Raphael J. Landovitz, Mark A. Marzinke
Summary: We developed and validated a LC-MS/MS assay for quantifying CAB and RPV from DBS, and derived conversion factors to estimate plasma concentrations from spotted blood.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Carmel E. Heiland, Magnus Ericsson, Anton Pohanka, Lena Ekstrom, Alexandre Marchand
Summary: This study investigated the use of dried blood spots (DBS) as a matrix for doping control and found optimal conditions for detecting prohibited erythropoietin receptor agonists (ERAs) and endogenous erythropoietin (EPO) from DBS. The results showed that 60 μl DBS could detect the main ERAs at concentrations close to the minimum required performance levels (MRPLs) set by WADA for serum/plasma samples. Storage temperature did not affect ERA detection. Compared with urine, DBS showed less variation in the detection of endogenous EPO.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Sara Amalie Solheim, Andreas Thomas, Thomas Kamm Ringsted, Mario Thevis, Andreas Breenfeldt Andersen, Henrik Holm-Sorensen, Nikolai B. Nordsborg, Jakob Morkeberg
Summary: Dried blood spot (DBS) testing is a fast, easy and minimally invasive method for collecting microvolumes of blood, and it has particular relevance in anti-doping control. This study demonstrates the feasibility of using alternative sampling sites and matrices for ephedrine analysis in DBS testing.
DRUG TESTING AND ANALYSIS
(2022)
Article
Engineering, Chemical
Alexander Chernonosov, Liliya Aksenova, Vladimir Koval
Summary: This study aimed to develop and validate a rapid, sensitive, and robust LC-HRMS method for the quantification of apixaban in DPSs. Validation results showed good accuracy and precision, demonstrating the method's selectivity and robustness.
Article
Multidisciplinary Sciences
Arline T. Geronimus, John Bound, Colter Mitchell, Aresha Martinez-Cardoso, Linnea Evans, Landon Hughes, Lisa Schneper, Daniel A. Notterman
Summary: Different specimen types show varying associations between telomere length (TL) and social constructs, which can sometimes qualitatively alter estimates of the magnitude or direction of a theorized relationship.
Article
Chemistry, Analytical
Tamin Tai, Orsolya Karacsony, Vera Bocharoya, Gary J. Van Berkel, Vilmos Kertesz
ANALYTICAL CHEMISTRY
(2016)
Article
Biochemical Research Methods
John F. Cahill, Vilmos Kertesz, Tiffany Porta, J. C. Yves LeBlanc, Ron M. A. Heeren, Gary J. Van Berkel
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2018)
Article
Biochemical Research Methods
John F. Cahill, Muneeba Khalid, Scott T. Retterer, Courtney L. Walton, Vilmos Kertesz
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2020)
Article
Biochemical Research Methods
Vilmos Kertesz, John F. Cahill, Bernadeta R. Srijanto, Charles P. Collier, Marissa Vavrek, Bingming Chen
Summary: The novel approach of combining laser ablation with the LA-dropletProbe automated liquid surface sampling system provides an effective method for sub-mm resolution quantitative drug distribution analysis. The use of parylene C coating on tissue prior to analysis, combined with the novel sampling system, allows for accurate quantification and detection of drugs and metabolites in tissue with high resolution.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Letter
Electrochemistry
Balazs B. Berkes, Vilmos Kertesz, Akos Kriston
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
(2021)
Article
Biochemical Research Methods
Vilmos Kertesz, John F. Cahill, Bernadeta R. Srijanto, Charles P. Collier, Marissa Vavrek, Bingming Chen
Summary: This study demonstrated the use of an integrated laser ablation-dropletProbe-mass spectrometry system for absolute quantification of propranolol in thin tissue sections, showcasing high resolution drug quantitation analysis capabilities that will be valuable for applications needing to understand the spatial distribution of small molecules in tissue.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Article
Chemistry, Inorganic & Nuclear
Aohan Hu, Megan E. Simms, Vilmos Kertesz, Justin J. Wilson, Nikki A. Thiele
Summary: Radioisotopes of metallic elements, or radiometals, are widely used in nuclear medicine. Recently, a new class of macrocyclic chelators with dual size selectivity was reported. A specific chelator called py(2)-macrodipa was found to have enhanced thermodynamic affinity for Ln(3+) and improved kinetic stability for light, large Ln(3+) ions. Moreover, py(2)-macrodipa was shown to effectively incorporate Ac-225(3+) and form a stable complex in human serum.
INORGANIC CHEMISTRY
(2022)
Article
Biochemical Research Methods
Courtney L. Walton, Muneeba Khalid, Amber N. Bible, Vilmos Kertesz, Scott T. Retterer, Jennifer Morrell-Falvey, John F. Cahill
Summary: The plant rhizosphere is a complex environment where the exchange of molecular signals between plants, microbes, and fungi drives the development of the biological system. Exogenous compounds in the rhizosphere play a crucial role in plant-microbe interactions, growth and development, and the spatiotemporal distribution of molecular components. A methodology for in situ measurement of amino acids from bacterial biofilms and plant roots using liquid microjunction-surface sampling probe-mass spectrometry was successfully developed.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Correction
Chemistry, Inorganic & Nuclear
Aohan Hu, Megan E. Simms, Vilmos Kertesz, Justin J. Wilson, Nikki A. Thiele
INORGANIC CHEMISTRY
(2022)
Article
Biochemical Research Methods
John F. Cahill, Vilmos Kertesz, Patricia St-Vincent, Hannah Valentino, Erin Drufva, Nikki Thiele, Joshua K. Michener
Summary: In this study, robotic autosampler and mass spectrometry techniques were employed to efficiently screen enzymatic degradation of polyamides. Optimal conditions for PA6 and PA66 hydrolysis were determined through 108 experiments, and product concentrations and conversion rates were quantified using mass spectrometry. This methodology can be used to evaluate the degradation efficiency of engineered enzyme libraries.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Vilmos Kertesz, Muneeba Khalid, Scott T. Retterer, John F. Cahill
Summary: This study presents a novel strategy to optimize the number and location of sampling sites on microporous membrane-covered microfluidic devices, taking into consideration the channel structure of the device. This strategy reduces the acquisition time and minimizes stress on plants during in situ analysis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
John F. Cahill, Vilmos Kertesz
Summary: In this study, a rapid droplet sampling interface (RDSI) electrospray ionization mass spectrometry system was developed for high-throughput, low-volume, noncontact characterization of liquids. The system combines droplet ejection with an open-face microflow capillary to achieve effective mixing and electrospray. With the continuous flow of carrier solvent, the system can achieve a sampling rate of 5 Hz and a lower limit of quantification of 15 nM for propranolol. The analyte-solvent mixing ratios can be controlled by adjusting droplet positioning along the capillary.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Inorganic & Nuclear
Megan E. Simms, Megan M. Sibley, Darren M. Driscoll, Vilmos Kertesz, Joshua T. Damron, Alexander S. Ivanov, Frankie D. White, Nikki A. Thiele
Summary: Targeted alpha therapy using Ra-223 shows great potential for soft-tissue cancers. However, the low complex stability of Ra-223 due to its challenging chelation chemistry hinders its clinical use. In this study, macropa-XL was synthesized and evaluated as a chelator for Ra-223, but it showed lower stability compared to the current state-of-the-art chelator, macropa.
INORGANIC CHEMISTRY
(2023)
Article
Biochemical Research Methods
Courtney L. Walton, Vilmos Kertesz, John F. Cahill
Summary: The study introduces a tethered, liquid-extraction-sampling interface for mass spectrometric surface sampling of 3D objects, which shows good analytical performance through optimized design.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Review
Biochemistry & Molecular Biology
Nicholas H. Oberlies, Sonja L. Knowles, Chiraz Soumia M. Amrine, Diana Kao, Vilmos Kertesz, Huzefa A. Raja
NATURAL PRODUCT REPORTS
(2019)