Review
Pharmacology & Pharmacy
Ying Hou, Hongren Yao, Jin -Ming Lin
Summary: Cellular heterogeneity is crucial for understanding tissue biology and disease pathophysiology. Single-cell metabolic analysis provides a technique to identify variations in RNA, proteins, metabolites, and drug molecules in cells. Microfluidics-based methods, in combination with various detection techniques, are used to evaluate single-cell metabolic changes in response to drug administration. These techniques have wide applications in pharmaceutical research.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Review
Obstetrics & Gynecology
Marta Venturas, Xingbo Yang, Denny Sakkas, Dan Needleman
Summary: Discusses the major challenge in assisted reproductive technology (ART) of selecting high-quality oocytes and embryos, and proposes the potential utility of metabolic measurements in aiding selection. Reviews recent work on noninvasive metabolic imaging using fluorescence lifetime imaging microscopy (FLIM) of cumulus cells, oocytes, and embryos. Argues that FLIM provides quantitative information on NAD(P)H and FAD+ concentrations and engagement with enzymes, making it a promising approach for oocyte and embryo selection.
HUMAN REPRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Cheng Zhao, Savana Biondic, Katherine Vandal, Asa K. Bjoerklund, Michael Hagemann-Jensen, Theresa Maria Sommer, Jesica Canizo, Stephen Clark, Pascal Raymond, Daniel R. R. Zenklusen, Nicolas Rivron, Wolf Reik, Sophie Petropoulos
Summary: The preconceptual, intrauterine, and early life environments can significantly impact the developmental trajectories and health outcomes of offspring. Assisted reproductive technologies using additives may alter blastocyst formation and affect the molecular profile and physiology of the embryo.
Article
Chemistry, Multidisciplinary
Yuan Ma, Kangfu Chen, Fan Xia, Randy Atwal, Hansen Wang, Sharif U. Ahmed, Lia Cardarelli, Irene Lui, Bill Duong, Zongjie Wang, James A. Wells, Sachdev S. Sidhu, Shana O. Kelley
Summary: The study demonstrates an integrated microfluidic chip using magnetic nanoparticles to efficiently capture single tumor cells, enable on-chip incubation, and facilitate in situ cell-surface protein expression analysis. By monitoring changes in the expression of multiple surface markers in response to CTC adherence, the researchers found fluctuations in the expression of Frizzled2 (FZD2) that reflected the microenvironment of the single cells. This platform has the potential to simultaneously screen multiple surface antigens in rare cells with single-cell resolution, providing further insights into biological heterogeneity and human disease.
Article
Developmental Biology
Wilhelm Bouchereau, Luc Jouneau, Catherine Archilla, Irene Aksoy, Anais Moulin, Nathalie Daniel, Nathalie Peynot, Sophie Calderari, Thierry Joly, Murielle Godet, Yan Jaszczyszyn, Marine Pratlong, Dany Severac, Pierre Savatier, Veronique Duranthon, Marielle Afanassieff, Nathalie Beaujean
Summary: In this study, a transcriptome analysis was conducted on rabbit preimplantation embryos, revealing common features between the transcriptome of rabbit epiblast cells and humans/non-human primates. Novel markers for deriving naive pluripotent stem cell lines were identified.
Article
Chemistry, Multidisciplinary
Channa Shapira, Daniel Itshak, Hamootal Duadi, Yifat Harel, Ayelet Atkins, Anat Lipovsky, Ronit Lavi, Jean Paul Lellouche, Dror Fixler
Summary: This paper investigates the use of the iterative multiplane optical properties extraction (IMOPE) technique to detect the penetration ability of nanodiamonds (NDs) in the skin, revealing a reduction in NDs presence at deeper layers and confirming the accuracy of the IMOPE results through additional experiments.
Article
Biochemistry & Molecular Biology
Leland B. Hyman, Clare R. Christopher, Philip A. Romero
Summary: Experimental methods focusing on individual properties of single cells have highlighted the significance of cell-to-cell variability in biological processes. SNAPD, a technology for single-cell nucleic acid profiling in droplets, provides a rapid, high-throughput approach for analyzing transcriptional markers in cell populations, allowing for cell classification and quantification in heterogeneous samples.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Chemistry, Analytical
Waraporn Suvannapruk, Max K. Edney, Dong-Hyun Kim, David J. Scurr, Amir M. Ghaemmaghami, Morgan R. Alexander
Summary: Studying the metabolic characteristics of macrophage subsets is important for understanding the human immune system's response to different stimuli, and utilizing the newly developed 3D OrbiSIMS instrument for cell analysis can provide useful information for metabolite profiling at the single-cell level, aiding in understanding the body's response to implanted devices and immune diseases.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Zengnan Wu, Ling Lin
Summary: Single-cell analysis is crucial for cell biology, drug discovery, and medical treatment. Conventional methods fail to analyze the real-time and continuous data of individual cells, while nanofluidics-based biochemical analysis offers advantages in sample processing and is widely used in single-cell capture, separation, and detection.
CHINESE CHEMICAL LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Weiwei Wang, Jinliang Guo, Jiayang Ma, Zhulin Wang, Lining Zhang, Zixu Wang, Min Meng, Chao Zhang, Fengli Sun, Yajun Xi
Summary: The transformation efficiency of wheat embryos was improved using a combination of special chemical and physical methods with the PureWheat technique. Scanning electron microscopy (SEM), transcriptome analysis and metabolome analysis were conducted to understand the reaction of immature embryos infected by Agrobacterium tumefaciens in PureWheat. The SEM analysis revealed that Agrobacterium tumefaciens was deposited under the damaged cortex of embryos and contacted the receptor cells to improve transformation efficiency. Transcriptome analysis showed that differentially expressed genes were involved in various metabolic pathways and signaling pathways, indicating the energy-demanding process of Agrobacterium infection. This study provides insights into the physiological and molecular changes of wheat embryos during the co-culture stage and sheds light on Agrobacterium-mediated transgenic experiments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Zhanhui Ou, Yu Deng, Yunhao Liang, Zhiheng Chen, Ling Sun
Summary: This study demonstrates the feasibility of using NGS for conducting PGT for thalassemia in affected embryos. The NGS analysis produced consistent results with prenatal diagnosis and gene analysis, and resulted in high clinical pregnancy and live birth rates.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Felix J. Hartmann, Dunja Mrdjen, Erin McCaffrey, David R. Glass, Noah F. Greenwald, Anusha Bharadwaj, Zumana Khair, Sanne G. S. Verberk, Alex Baranski, Reema Baskar, William Graf, David Van Valen, Jan Van den Bossche, Michael Angelo, Sean C. Bendall
Summary: This antibody-based method allows profiling of metabolic protein expression and regulation in single cells using mass spectrometry while characterizing cellular phenotype. The approach was validated using mass cytometry and applied to clinical samples, revealing tissue-restricted, metabolically repressed immune cells in human colorectal carcinoma. The combination with multiplexed ion beam imaging unveiled the spatial organization of metabolic programs in human tissues.
NATURE BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Chayakorn Petchakup, Paul Edward Hutchinson, Hui Min Tay, Sheng Yuan Leong, King Ho Holden Li, Han Wei Hou
Summary: This study presents a new method for studying lymphocytes based on impedance, which can quickly screen the responses of specific antigen-specific T lymphocytes and be used for the characterization of host immunity and the diagnosis of infectious diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Obstetrics & Gynecology
Cengiz Cinnioglu, Heather Glessner, Amy Jordan, Sydney Bunshaft
Summary: Noninvasive and minimally invasive preimplantation genetic testing for aneuploidy (PGT-A) has the potential to become the gold standard for embryonic chromosomal screening. The current research focuses on finding a reliable source of DNA without the need for embryo biopsy. Although the general concordance rates of noninvasive and minimally invasive PGT-A are promising, further research is needed before it can be widely used in clinics.
FERTILITY AND STERILITY
(2023)
Review
Obstetrics & Gynecology
Shuntaro Ikeda
Summary: This review summarizes the current status of genome-wide epigenetic profiling in mammalian preimplantation embryos, and suggests that the accumulated data can be used to improve the quality of these embryos.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2023)
Article
Cell & Tissue Engineering
James Spyrou, David K. Gardner, Alexandra J. Harvey
Article
Cell & Tissue Engineering
Jarmon G. Lees, Anne M. Kong, Yi C. Chen, Priyadharshini Sivakumaran, Damian Hernandez, Alice Pebay, Alexandra J. Harvey, David K. Gardner, Shiang Y. Lim
STEM CELLS INTERNATIONAL
(2019)
Article
Cell Biology
Harriet R. Manley, David L. Potter, John M. Heddleston, Teng-Leong Chew, M. Cristina Keightley, Graham J. Lieschke
JOURNAL OF LEUKOCYTE BIOLOGY
(2020)
Article
Obstetrics & Gynecology
Kathryn H. Gurner, Joseph J. Richardson, Alexandra J. Harvey, David K. Gardner
Summary: The study showed that VEGF-loaded silica supraparticles (V-SPs) can promote embryonic development in vitro, similar to the effect of free VEGF supplementation. This suggests that V-SPs could be a novel method for delivering therapeutic agents to support embryo development, potentially overcoming the limitations of VEGF stability in solution. Further investigation is needed to explore the clinical application of V-SPs in IVF.
HUMAN REPRODUCTION
(2021)
Article
Obstetrics & Gynecology
Kathryn H. Gurner, Jemma Evans, Jennifer C. Hutchison, Alexandra J. Harvey, David K. Gardner
Summary: This study suggests that lactate may play an important role in maternal-fetal dialogue, promoting endometrial receptivity and implantation. Further investigation of the role of lactate in infertile or compromised endometrium could potentially improve pregnancy success.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Obstetrics & Gynecology
Thi Truong, Alexandra J. Harvey, David K. Gardner
Summary: This study investigated the effect of combined antioxidants on mouse fetal gene expression. The results showed that both in-vitro culture in the presence of 20% oxygen and vitrification of blastocysts significantly perturbed fetal liver and placental gene expression. However, supplementation with A3 reduced the number of differentially expressed genes and altered biological processes, establishing a more in-vivo-like gene expression profile, particularly in the placenta. Additionally, A3 supplementation significantly reduced the expression of genes associated with pre-eclampsia and intrauterine growth restriction.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Obstetrics & Gynecology
David K. Gardner
Summary: By introducing more technologies in the ART laboratory, outcomes such as live birth rate, time to pregnancy, and automation can be improved. New technologies have the potential to assist more patients in becoming parents of healthy children in a shorter period of time.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Obstetrics & Gynecology
Emma G. Whatley, Thi T. Truong, Dagmar Wilhelm, Alexandra J. Harvey, David K. Gardner
Summary: Exposure to the ketone beta-hydroxybutyrate (beta OHB) impairs preimplantation mouse embryo development, metabolism, epigenetics, and post-transfer viability, with significant effects seen at ketogenic diet (KD)-relevant serum concentrations. These findings suggest a potential detrimental impact on embryo development and viability due to maternal diets that elevate beta OHB levels.
HUMAN REPRODUCTION
(2022)
Article
Obstetrics & Gynecology
Emma G. Whatley, Thi T. Truong, Alexandra J. Harvey, David K. Gardner
Summary: Research question: Does the ketone acetoacetate (AcAc) alone, or combined with 13-hydroxybutyrate (13OHB), impact mouse embryo development, metabolism, histone acetylation and viability? The study found that exposure to high levels of ketone acetoacetate and 13-hydroxybutyrate affects metabolism and histone acetylation in embryos, and leads to abnormal fetal development.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
David K. Gardner, Denny Sakkas
Summary: Our ability to maintain viable human embryos in vitro has greatly improved in the past 40 years, leading to higher implantation rates. The future development of embryo culture systems will benefit from new media formulations and more dynamic perfusion-based systems. Furthermore, the integration of artificial intelligence and biomarkers can predict embryo health and viability, improving the effectiveness and cost efficiency of assisted reproductive technology.
FERTILITY AND STERILITY
(2023)
Article
Multidisciplinary Sciences
Hanna J. McLennan, Adam J. Blanch, Samuel J. Wallace, Lesley J. Ritter, Shauna L. Heinrich, David K. Gardner, Kylie R. Dunning, Marty J. Gauvin, Allison K. Love, Jeremy G. Thompson
Summary: This study introduces a microfluidic chip that is designed and completely 3D-printed using two-photon polymerization (2PP). It consists of ten channels and ten retrievable cell sample seats, which can enhance cell-surface interactions at the nanoscale and support cell culture. This technology provides the precision needed for nanoliter fluidic devices constructed from multiple interlocking parts.
SCIENTIFIC REPORTS
(2023)
Article
Obstetrics & Gynecology
Emma G. Whatley, Thi T. Truong, Alexandra J. Harvey, David K. Gardner
Summary: In vitro exposure to ketone bodies fi-hydroxybutyrate (fiOHB) and acetoacetate (AcAc) has a sexually dimorphic impact on gene expression in the fetal liver and placenta of preimplantation mouse embryos.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
Jennifer C. Hutchison, Jemma Evans, Tracey A. Edgell, Guiying Nie, David K. Gardner, Lois A. Salamonsen
Summary: The study found that obese infertile women have elevated levels of advanced glycation end-products (AGE) in their uterine environment. The detrimental effects of AGE on endometrial epithelial cells can be reduced with therapeutics and replicated in a more physiologically relevant primary model (organoids).
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Meeting Abstract
Obstetrics & Gynecology
H. Elzeiny, F. Agtesta, F. Cattrall, J. Stevens, D. K. Gardner
HUMAN REPRODUCTION
(2022)
Article
Developmental Biology
Kathryn H. Gurner, Thi T. Truong, Alexandra J. Harvey, David K. Gardner
MOLECULAR HUMAN REPRODUCTION
(2020)