Article
Biochemical Research Methods
Andaravaas Patabadige Jude P. Vaas, Joselito P. Quirino
Summary: A stacking technique using CTAB and SDS was proposed to enhance the detection sensitivity of capillary zone electrophoresis (CZE) with UV detection. The method involves enriching and releasing analytes from the inner walls of a fused silica capillary by forming catanionic micelles, leading to improved detection sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemistry & Molecular Biology
Veronika Solinova, Petra Sazelova, Alice Masova, Jiri Jiracek, Vaclav Kasicka
Summary: The growing resistance of bacteria to conventional antibiotics has led to the search for new antimicrobial agents, with insect antimicrobial peptides (AMPs) showing promise as potential therapeutics. A dipeptide beta-alanyl-tyrosine (beta-Ala-Tyr) in insect toxins has exhibited antibacterial activity, but requires purification before testing for other antimicrobial activities. A method using capillary zone electrophoresis (CZE) was developed for analysis, and a preparative purification of beta-Ala-Tyr was achieved using free-flow zone electrophoresis (FFZE).
Article
Chemistry, Analytical
Daniel Sarkozy, Andras Guttman
Summary: A simple and widely applicable coaxial sheath flow reactor interface (CSFRI) is introduced for easy and robust connection of liquid-phase microseparation methods to mass spectrometric detection. The interface allows the utilization of non-MS friendly buffer components without significant ion suppression and supports stable electrospray. In SDS capillary agarose gel electrophoresis mode, addition of gamma-cyclodextrin efficiently removed the SDS content of the sample and the background electrolyte in the flow reactor section while maintaining good separation efficiency and decreasing ion suppression.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Andaravaas Patabadige Jude P. Vaas, Raymond B. Yu, Joselito P. Quirino
Summary: Cyclodextrins are proposed as a pseudophase in pseudophase-to-pseudophase microextraction in capillary zone electrophoresis. This method, known as CD to admicelle ME, involves the injection of a dilute analyte solution into the capillary, forming admicelles at the interface. CD solution is then injected to release the analytes from the capillary surface, resulting in concentration of the analytes. The method shows enhanced sensitivity and has potential for analysis of urine samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Youngbok Lee, Jae-Sung Kwon
Summary: Microfluidic free-flow electrophoresis (mu FFE) is a promising technique for real-time separation and detection of analytes in proteomics. This review provides an overview of the principle, fabrication, detection methods, and applications of mu FFE, particularly focusing on microfluidic free-flow zone electrophoresis and microfluidic free-flow isoelectric focusing.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2022)
Article
Chemistry, Analytical
Amir Sohail, Xiaoteng Jiang, Amir Wahid, Huiyu Wang, Chengxi Cao, Hua Xiao
Summary: Cancer cells are heterogeneous, and their subpopulations contain distinct biological information. In this study, free-flow zone electrophoresis was used to separate lung cells, and quantitative proteomics analysis was performed. The results showed that our method can distinguish cancer cell subpopulations with different metastatic phenotypes.
ANALYTICA CHIMICA ACTA
(2022)
Article
Computer Science, Interdisciplinary Applications
A. Karakhi, N. Lakouari, A. Khallouk, K. Bentaleb, R. Marzoug, H. Ez-Zahraouy
Summary: This paper investigates the impact of speed limit zones on traffic flow and energy dissipation. The study reveals that traffic flow and energy dissipation are strongly influenced by the length of the speed limit zone and the speed adopted within it. The microscopic effects of the speed limit zone are also analyzed, including the evaluation of platoon density and length induced by the zone. Additionally, the energy dissipation can be used as an indicator of traffic phases.
INTERNATIONAL JOURNAL OF MODERN PHYSICS C
(2023)
Article
Biochemical Research Methods
Byoung Joo Kwak, Hanok Kim, Nokyoung Park, Jong Hoon Hahn
Summary: A continuous-flow microchip for high-accuracy DNA analysis has been developed, allowing serial consecutive analysis of multiple amplified DNA samples. The use of electrokinetic co-injection with size marker enables accurate size determination of the DNA samples, while minimizing cross-contamination between samples. This microchip shows potential as a platform for fast and highly accurate DNA analysis with shorter separation times compared to traditional gel electrophoresis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Miroslava Stastna, Karel Slais
Summary: In this study, a method and instrumentation were developed to separate and fractionate immunoglobulins from bovine colostrum. The separated immunoglobulin G was collected and confirmed using various techniques. The results demonstrated a successful and efficient separation of immunoglobulin G from other immune proteins.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Physics, Applied
Tao Wang, Zhiyue Zuo, Lang Li, Peng Huang, Ying Guo, Guihua Zeng
Summary: Accurate clock synchronization is crucial for quantum information processing tasks. Proposal of continuous-variable quantum key distribution (CV QKD) protocol has benefited from the natural synchronization signals provided by the local oscillator (LO). However, the use of LO poses security vulnerabilities. To mitigate this, we propose a virtual clock synchronization method based on the interpolation algorithm, which extracts clock information from the measured quadrature of the quantum signal instead of the traditional LO. Numerical results demonstrate the robustness and potential of our method in real CV QKD implementation.
PHYSICAL REVIEW APPLIED
(2022)
Article
Chemistry, Analytical
Kevin Jooss, Luis F. Schachner, Rachel Watson, Zachary B. Gillespie, Sarah A. Howard, Marcus A. Cheek, Matthew J. Meiners, Amin Sobh, Jonathan D. Licht, Michael-Christopher Keogh, Neil L. Kelleher
Summary: The study introduces a new platform for separating and characterizing whole nucleosomes and their modifications, aiding in understanding epigenetic regulatory mechanisms. The platform offers low sample requirements and costs, with high detection sensitivity.
ANALYTICAL CHEMISTRY
(2021)
Article
Electrochemistry
Yingchao Liu, Yuanyu Huang, Mengxi Wu, Siyuan Kong, Weiqian Cao, Shunxiang Li, Guoquan Yan, Baohong Liu, Pengyuan Yang, Quanqing Zhang, Liang Qiao, Huali Shen
Summary: In this study, a novel microfluidic free-flow paper electrochromatography (mu-FFPE) method was developed by combining mu-FFE with filter paper-based chromatography, enabling continuous separation of charged analytes. The addition of filter paper as the stationary phase improved the stability of the separation system. The mu-FFPE showed great potential in the separation and analysis of various biological molecules.
Article
Biochemical Research Methods
Miroslava Stastna, Karel Slais
Summary: A preparative instrumentation based on bidirectional isotachophoresis/electrophoresis in continuous divergent flow was developed for efficient separation of samples. The instrument can be easily optimized and tailored for various types of samples, providing fast and effective separation without the need for intermediate steps.
Article
Optics
Kai-Sheng Yu, Chung-Wei Cheng, An-Chen Lee, Wei-You Jhang Jian, Wen-Cheng Chang, Tsung-Wei Chang, Mi-Ching Tsai
Summary: The study demonstrates that using LPBF systems with three-beam lasers and optimizing process parameters can significantly improve the magnetic properties of NdFeB components and reduce processing time.
OPTICS AND LASER TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Aodong Zhang, Jian Xu, Xiaolong Li, Zijie Lin, Yunpeng Song, Xin Li, Zhenhua Wang, Ya Cheng
Summary: This study proposes a method for the design and fabrication of a high-throughput micro free-flow electrophoresis (mu FFE) chip using laser-assisted chemical etching, electrode integration, and low-temperature bonding. The careful design of the height ratio of the separation chamber and electrode channels combined with a high flow rate of buffer solution allows for efficient removal of electrolysis-generated bubbles and enhances separation stability.
Article
Materials Science, Multidisciplinary
Jedrzej Winczewski, Manuel Herrera, Clement Cabriel, Ignacio Izeddin, Stefan Gabel, Benoit Merle, Arturo Susarrey Arce, Han Gardeniers
Summary: This study develops a tailor-made organo-metallic resin suitable for two-photon lithography, which enables the additive manufacturing of 3D luminescent microarchitectures emitting light in the visible range. Through thermal treatment in an oxygen-rich atmosphere, silicon-free ZrO2 structures can be created using this resin, which is compatible with a luminescent activator and allows for tuning of the optical properties.
ADVANCED OPTICAL MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Ali Margot Huerta-Flores, Francisco Ruiz-Zepeda, Cavit Eyovge, Jedrzej P. Winczewski, Matthias Vandichel, Miran Gaberscek, Nicolas D. Boscher, Han J. G. E. Gardeniers, Leticia M. Torres-Martinez, Arturo Susarrey-Arce
Summary: This study synthesized a new RuO2/CuxO/Ta2O5/SrZrO3 heterostructure that achieved a high H-2 production of 5164 mu mol g(-1) h(-1) under simulated solar light, which was significantly higher than other heterostructures. The addition of the binary catalyst CuxO resulted in higher H-2 production. The presence of the binary catalyst improved the charge carrier transport and the solar to hydrogen conversion efficiency.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Duy Le-Anh, Ashit Rao, Amy Stetten, Subhash Ayirala, Mohammed Alotaibi, Michel Duits, Han Gardeniers, Ali AlYousef, Frieder Mugele
Summary: In this study, a 2.5D glass microchannel coated with calcite particles was used to mimic the multiple porosity length scales and surface heterogeneity of carbonate reservoirs. The results showed that flooding with high-salinity water at varying temperatures and durations can effectively enhance the liberation of crude oil in the reservoir. The experiment also revealed that wall roughness in the microscale model plays a more significant role in oil retention than surface chemistry.
Article
Materials Science, Multidisciplinary
M. A. Rodriguez-Olguin, C. Flox, R. Ponce-Perez, R. Lipin, F. Ruiz-Zepeda, J. P. Winczewski, T. Kallio, M. Vandichel, J. Guerrero-Sanchez, J. G. E. Gardeniers, N. Takeuchi, A. Susarrey-Arce
Summary: We report the finding that incorporating chlorine into NiO can transform it into an active electrocatalyst for the reduction of CO2 to formate. Chlorine-containing NiO particles synthesized by a solid-state method exhibit high Faradaic efficiency and current density for formate production. XPS and STEM-EDX analysis confirm the presence of Ni3+, Ni2+, and Cl species. DFT modeling and Pourbaix diagrams provide insights into the role of Cl on the NiO surface.
APPLIED MATERIALS TODAY
(2022)
Article
Chemistry, Analytical
Amel Meziani, Sandrien Verloy, Ouassila Ferroukhi, Sebastien Roca, Aurelien Curat, Severine Tisse, Valerie Peulon-Agasse, Han Gardeniers, Gert Desmet, Pascal Cardinael
Summary: This study investigated the use of a dedicated gas chromatography column with radially elongated pillars as the second column in a comprehensive two-dimensional gas chromatography system. The study found that the use of a vacuum pressure program significantly improved efficiency, and identified optimal experimental conditions for the best performance of the column.
ANALYTICAL CHEMISTRY
(2022)
Article
Electrochemistry
Akash Raman, Pablo Penas, Devaraj van der Meer, Detlef Lohse, Han Gardeniers, David Fernandez Rivas
Summary: The presence of bubbles in gas-evolving electrolytic processes can alter the mass transport of gaseous products and induce overpotential penalties at the electrode. However, bubble formation can also alleviate the overpotential by lowering the concentration of dissolved gas. This study investigates the concentration-lowering effect of hydrogen bubbles in alkaline-water electrolysis and their impact on the half-cell potential.
ELECTROCHIMICA ACTA
(2022)
Article
Engineering, Electrical & Electronic
L. Capuano, J. W. Berenschot, R. M. Tiggelaar, M. Feinaeugle, N. R. Tas, J. G. E. Gardeniers, G. R. B. E. Romer
Summary: This paper presents a processing technique for sapphire that combines laser-induced amorphization and selective wet etching. Microstructures can be achieved on the surface and in the bulk of sapphire substrates using this technique. Ultrashort laser pulses are used to transform the sapphire structure from crystalline to amorphous, and an etchant consisting of sulfuric acid and phosphoric acid is used to selectively remove the amorphized part. This method allows the realization of structures that are impossible to achieve with conventional etchants.
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2022)
Article
Engineering, Chemical
Ward Van Geite, Ignaas S. M. Jimidar, Han Gardeniers, Gert Desmet
Summary: A universal and rapid dry assembly strategy is proposed to attain ordered arrays of micro- and nanoparticles. This method involves shooting agglomerated monodisperse silica, polystyrene, or PMMA powder microspheres against an impact plating and attracting them towards the pores of a silicon membrane device by applying a vacuum force. Excess particles are removed by brushing. The optimized conditions can be used to attain any desired 2-D particle arrangement on soft surfaces.
Article
Multidisciplinary Sciences
Julian Schmitz, Birgit Stute, Sarah Taeuber, Dietrich Kohlheyer, Eric von Lieres, Alexander Gruenberger
Summary: This paper presents an easy-to-implement cell retention concept to prevent cells from leaving microfluidic cultivation chambers, enabling reliable single-cell growth studies.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Keitaro Kasahara, Markus Leygeber, Johannes Seiffarth, Karina Ruzaeva, Thomas Drepper, Katharina Noeh, Dietrich Kohlheyer
Summary: Microfluidic cultivation devices with O2 control enable in-depth studies of the relationship between O2 availability and microbial physiology at the single-cell level. Time-lapse microscopy combined with deep learning analysis techniques can provide valuable insights into microbiology. A comprehensive experimental approach using a gas-permeable microfluidic chip, a 3D-printed mini-incubator, and fluorescence lifetime imaging allows for spatiotemporal single-cell analysis under controlled O2 conditions.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Dirk Jonker, Ketki Srivastava, Marta Lafuente, Arturo Susarrey-Arce, Ward van der Stam, Albert van den Berg, Mathieu Odijk, Han J. G. E. Gardeniers
Summary: In this study, gold nanoparticles with a diameter of 67 +/- 6 nm supported by vertically aligned shell-insulated silicon nanocones were formed for ultralow variance SERS. The morphology and optical properties of the substrates were assessed through various characterization techniques. The SERS activity was measured and compared to other lithographically derived assemblies. The low variance of our substrates makes them suitable for many potential SERS applications.
ACS APPLIED NANO MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Ignaas S. M. Jimidar, Wojciech Kwiecinski, Gijs Roozendaal, E. Stefan Kooij, Han J. G. E. Gardeniers, Gert Desmet, Kai Sotthewes
Summary: Contact electrification is the exchange of electrical charges between two surfaces when they are in contact. This principle has been used in triboelectric nanogenerators (TENGs) to generate electricity. This study demonstrates the important role of water in the charge exchange process between two insulating surfaces with different wettability. The charging process is faster and acquires more charge with increasing relative humidity, especially beyond RH = 40%. The study also reveals the effect of humidity on the charging time constant. The findings contribute to the understanding of how humidity levels influence the charging process and have implications for the design of more efficient TENGs and other eco-energy harvesting devices.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Environmental
Akash Raman, Carla Corina dos Santos Porto, Han Gardeniers, Cintia Soares, David Fernandez Rivas, Natan Padoin
Summary: This study simulated a system consisting of spatially-decoupled electrolytic bubbles and analyzed the growth and departure process of electrolytic bubbles and their impact on gas evolution efficiency.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemical Research Methods
Bert Vankeerberghen, Sandrien Verloy, Ignaas S. M. Jimidar, Han Gardeniers, Gert Desmet
Summary: We introduce a novel concept of producing ordered beds of spherical particles for liquid chromatography. This concept involves positioning spherical particles individually or stacked in micromachined pockets, forming an interconnected array of micro-grooves. Our breakthrough involves achieving uniform filling of the microgroove arrays with spherical particles using a rubbing approach. Numerical calculations show the advantages of this new concept over conventional packed beds in terms of order and reduced flow resistance.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Multidisciplinary
Jedrzej Winczewski, Manuel Herrera, Han Gardeniers, Arturo Susarrey-Arce
Summary: This paper presents the additive manufacturing of three-dimensional structured phosphors emitting red, green, blue, and white, which allows advanced applications in optics and photonics. This development is a step forward in realizing additive color synthesis within complex architectures relevant to integrated optics or light-emitting sources.
CHEMICAL COMMUNICATIONS
(2023)
