Journal
ANALYTICAL CHEMISTRY
Volume 80, Issue 1, Pages 107-114Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac071263f
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Funding
- NATIONAL INSTITUTE ON AGING [P30AG013319] Funding Source: NIH RePORTER
- NIA NIH HHS [AG13319] Funding Source: Medline
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This work describes an approach to differential metabolomics that involves stable isotope labeling for relative quantification as part of sample analysis by two-dimensional gas chromatography/mass spectrometry (GC x GC/MS). The polar metabolome in control and experimental samples was extracted and differentially derivatized using isotopically light and heavy (D-6) forms of the silylation reagent N-methyl-N-tert-butyldimethyl-silyl)trifluoroacetamide (MTBSTFA). MTBSTFA derivatives are of much greater hydrolytic stability than the more common trimethylsilyl derivatives, thus diminishing the possibility of isotopomer scrambling during GC analysis. Subsequent to derivatization with MTBSTFA, differentially labeled samples were mixed and analyzed by GC x GC/MS. Metabolites were identified, and the isotope ratio of isotopomers was quantified. The method was tested using three classes of metabolites; amino acids, fatty acids, and organic acids. The relative concentration of isotopically labeled metabolites was determined by isotope ratio analysis. The accuracy and precision, respectively, in quantification of standard mixtures was 9.5 and 4.77% for the 16 amino acids, 9.7 and 2.83% for the mixture of 19 fatty acids, and 14 and 4.53% for the 20 organic acids. Suitability of the method for the examination of complex samples was demonstrated in analyses of the spiked blood serum samples. This differential isotope coding method proved to be an effective means to compare the concentration of metabolites between two samples simultaneously.
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