Article
Biophysics
Xiaotong Meng, Danny O'Hare, Sylvain Ladame
Summary: With the increasing demand for a new generation of nucleic acid tests, easily miniaturized electrochemical sensors show the greatest promise as they can be cost-effective, highly sensitive, and specific. These sensors rely on the immobilization of sequence-specific hybridization probes onto conductive materials or electrodes. This review covers various strategies for immobilizing nucleic acid probes onto different solid electrode materials, including both physical and chemical immobilization techniques.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Jinying Dong, Xiaoya Wu, Qiushi Hu, Chongsi Sun, Jiahao Li, Peng Song, Yan Su, Lei Zhou
Summary: This study proposes a simple, scalable, and general idea and solution for the rapid detection of pathogenic microorganisms and biosecurity monitoring. By designing an electrochemical/optical signal probe and integrating CRISPR/Cas technology and recombinase polymerase amplification, fast and accurate detection of pathogenic microorganisms is achieved.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Ruijiang Liu, Yanling Zhang, Min Liu, Yun Ni, Yao Yue, Shaobo Wu, Shasha Li
Summary: Considering the high cost and tedious process of gene sequencing, a portable and efficient sensor for the TP53 gene is urgently needed. In this study, a novel electrochemical sensor was developed using magnetic peptide nucleic acid-modified Fe3O4/alpha-Fe2O3@Au nanocomposites to detect the TP53 gene. The sensor exhibited high-affinity binding of PNA to DNA strands, leading to current changes and enabling the detection of TP53 gene variations.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Qitian Lin, Junye Wu, Lili Jiang, Dexian Kong, Chao Xing, Chunhua Lu
Summary: The study developed an electrochemical biosensor based on Mg2+-dependent DNAzyme for simultaneous detection of miRNA-21 and miRNA-141, showing high sensitivity and selectivity. The biosensor offers advantages such as simple preparation and enzyme-free signal amplification, making it suitable for various bioanalysis and clinical diagnostics.
Article
Chemistry, Analytical
Zhen Song, Yihui Ma, Min Chen, Adriano Ambrosi, Caifeng Ding, Xiliang Luo
Summary: An antifouling electrochemical biosensor was developed using electropolymerized polyaniline nanowires and newly designed peptides for COVID-19 N-gene detection, demonstrating a low detection limit and wide linear range. The sensor showed high sensitivity and accurate detection capability in complex systems.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Qiong Hu, Luofeng Su, Yanyu Huang, Zhuohua Chen, Xiaojing Cao, Yilin Luo, Dongdong Qin, Li Niu
Summary: An ultrasensitive peptide nucleic acid (PNA)-based electrochemical DNA biosensor was reported using coenzyme-mediated electro-grafting of polymers for signal amplification, showing great promise in sequence-specific detection of DNA targets at ultralow concentrations.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Philip Gillespie, Robert B. Channon, Xiaotong Meng, Md Nazmul Islam, Sylvain Ladame, Danny O'Hare
Summary: Short single-stranded nucleic acids found in bodily fluids, particularly miR-141, have emerged as minimally invasive biomarkers for diseases such as cancer. This study introduces a novel sensing strategy using electrochemically active molecular probes based on PNA scaffolds for detecting short nucleic acids, demonstrating promising potential for fast, low-cost, quantitative, and sequence-specific detection of nucleic acids from liquid biopsies through EOTRs.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Shu Tian, Xiaoya Wang, Chenchen Wang, Liqun Li, Wei Wei
Summary: In this study, a simple and label-free method using differential pulse voltammetry (DPV) was proposed for the sensitive detection of menthol. Silica microspheres (SiO2) were introduced for better modification and enrichment of ferrocene (Fc) on the electrode surface to improve detection sensitivity. The electrochemical signal of Fc was inhibited due to the inclusion action of Fc and ss-cyclodextrins (ss-CD), but showed remarkable recovery in the presence of menthol. The recovered electrochemical signal increased linearly with the concentration of menthol, providing a high sensitivity for quantitative detection.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Mingyuan Zhao, Xiaoli Zhao, Ritong Sun, Jian Zhang, Haixia Wang, Chao Shi, Cuiping Ma, Yong Li
Summary: A simple electrochemical DNA biosensor was developed for rapid and specific determination of DNA using electrical potential-assisted hybridization. The biosensor showed rapid detection capability with controlled potential, linear relationship with target DNA concentrations, and good specificity for distinguishing mismatched sequences. It is a promising diagnostic tool for DNA detection.
ANALYTICAL LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Jie Hao, Zhenbo Wang, Yafei Li, Yaru Deng, Yanru Fan, Yu Huang
Summary: The water solubility problem of the synthesized ferrocene appended naphthalimide derivative (FND) was solved by the interaction with alpha-cyclodextrin (alpha-CD), which also amplified the signal of the DNA biosensor.
BIOELECTROCHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Qiong Hu, Yilin Luo, Xiaojing Cao, Zhuohua Chen, Yanyu Huang, Li Niu
Summary: A method for highly selective and amplified sensing of ultralow-abundance nucleic acids using PNA recognition probe and BERP-based amplification strategy is presented. The method is cost-effective, easy to operate, highly selective, and has a low detection limit.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biophysics
Yu Xue, Haopu Xie, Yu Wang, Sinuo Feng, Jian Sun, Jianshe Huang, Xiurong Yang
Summary: In this study, a novel electrochemical/fluorescent dual-mode biosensor was developed for sensitive and accurate detection of target nucleic acids. The biosensor utilized functional nucleic acids to achieve self-assembly and cascaded amplification, significantly improving the sensitivity and accuracy. The research showed promising applications in bioanalysis and early disease diagnosis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Maliana El Aamri, Hasna Mohammadi, Aziz Amine
Summary: A smartphone colorimetric and electrochemical-based miRNA sensor was developed to detect the biomarker miRNA-21 for early cancer diagnosis in blood and body fluids. The combination of acid treatment and molybdate reaction allowed for accurate measurement of miRNA-21 concentration. The sensor showed a good linear range and low detection limits, and was successfully used to analyze miRNA-21 in human serum samples.
MICROCHEMICAL JOURNAL
(2022)
Article
Nanoscience & Nanotechnology
Zhenmao Chen, Yijing Ai, Weiqiang Yang, Li Niu, Qingxiang Wang, Feng Gao
Summary: A novel immobilization-and label-free electrochemical strategy has been developed for the detection of miRNA let-7a using magnetic Fe3O4@hydroxyapatite (Fe3O4@HAP) and the specific adsorption of a duplex structure. With the introduction of the label-free signal molecule Nile blue (NB) and its intercalation into the pDNA-miRNA duplex, a strong sensing signal is achieved without the need for nuclease-assisted cyclic amplification. The sensing system shows a wide linear range (1.0 fM to 100 nM) and a low detection limit (0.051 fM) for miRNA let-7a analysis, and exhibits high specificity.
ACS APPLIED NANO MATERIALS
(2023)
Article
Plant Sciences
Yunzhi Lin, Chen Ye, Xingzhu Li, Qinyao Chen, Ying Wu, Feng Zhang, Rui Pan, Sijia Zhang, Shuxia Chen, Xu Wang, Shuo Cao, Yingzhen Wang, Yi Yue, Yongsheng Liu, Junyang Yue
Summary: A high-quality genome is important for studying functional, evolutionary, and comparative genomics. However, there is a lack of bioinformatic tools for automatically constructing and characterizing T2T genomes. The quarTeT toolkit addresses this issue by providing user-friendly modules for genome assembly and characterization.
HORTICULTURE RESEARCH
(2023)
Article
Biochemical Research Methods
Alan F. Rodriguez-Serrano, I-Ming Hsing
Summary: The study introduces a new modular biosensing approach for detecting environmental pollutants, utilizing DNA signal amplifiers and transducer circuits controlled by allosteric transcription factors and their ligands. By leveraging competition between allosteric proteins and endonucleases, the researchers successfully built optical biosensors for detecting tetracyclines and macrolides in water. The minimal, fast, and single-step biosensors were shown to detect antibiotics in nanomolar levels and have great potential for monitoring water contaminants.
ACS SYNTHETIC BIOLOGY
(2021)
Review
Chemistry, Physical
Asmita Veronica, I-Ming Hsing
Summary: The production of bioelectronics should adopt sustainability concepts, and green electronics is gaining attention as a new concept. Silk, as a green material with innate piezoelectricity, can be used to manufacture self-powered bioelectronic systems.
Article
Materials Science, Multidisciplinary
Yue Li, Alan F. Rodriguez-Serrano, Sin Yu Yeung, I-Ming Hsing
Summary: This study presents an adhesive, highly stretchable and conformal (ASC) patch for long-term ambulatory ECG monitoring, offering a revolutionary approach to cardiovascular disease diagnosis. The ASC patch demonstrates enhanced mechanical and electrical properties, providing stable high-quality ECG signals in various conditions. This work paves the way for scalable wearable ECG devices and new opportunities in medical applications in soft electronics.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Chemistry, Analytical
Xinyu Zhuang, Xiao Lu, Henson L. Lee Yu, I-Ming Hsing
Summary: A unique barcoded primer-assisted sample-specific pooled testing strategy (Uni-Pool) was developed to improve the efficiency of pooled testing during the COVID-19 pandemic. This strategy eliminates the need for retesting and allows for accurate and sensitive detection of positive and negative samples. The method was successfully validated and demonstrated for large-scale screening.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xiao Lu, Henson Lee Yu, Haosi Lin, Yumeng Cao, I-Ming Hsing
Summary: In this study, we developed a one-pot loop probe-mediated isothermal amplification scheme (oLAMP) that enables rapid and specific detection of pathogens in communicable diseases and food samples. This platform offers a scalable and reliable diagnostic pathway for decentralized detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Nanoscience & Nanotechnology
Shiyao Wang, Ailin Qin, Li Yin Chau, Eunice W. T. Fok, Mei Yue Choy, Christopher J. Brackman, Gilman K. H. Siu, Chien-Ling Huang, Shea Ping Yip, Thomas M. H. Lee
Summary: Loop-mediated isothermal amplification (LAMP) has received substantial attention for decentralized nucleic acid testing due to its simple temperature control and short assay time. However, the existing amplification result reporter probes have limitations. This study demonstrates a new reporter probe based on CdSeS/ ZnS quantum dots with superior photostability, enabling single-copy sensitivity. The amine-QD-based one-step LAMP assay advances the field of QD-based nucleic acid amplification assays by achieving compatibility and universality.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Medicinal
Alan Fernando Rodriguez Serrano, I-Ming Hsing
Summary: Understanding the interactions between aptamers and ligands is important for designing aptamer-based systems. This study used independent simulations and multiple metastable conformations to accurately predict binding sites, providing valuable insights for aptamer design and applications.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Chemistry, Analytical
Xiao Lu, Haosi Lin, Xianzhen Feng, Dongling Long, Guiqing Yang, I-Ming Hsing
Summary: Etaq-PCR is a portable real-time polymerase chain reaction (PCR) platform that utilizes an electroactive hydrolysis probe design and immobilization-free electrochemical readout. It integrates cost effective commercial instruments and thermally stable cycling into a minimal device, enabling real-time monitoring of PCR amplification. The construction of the Etaq-PCR platform does not require complex electrode surface treatment or micro-fabrication, making it easy to construct a portable PCR platform. Etaq-PCR meets the WHO ASSURED criteria and has sufficient sensitivity, selectivity, and rapidness for point-of-care diagnosis systems.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Sin Yu Yeung, Asmita Veronica, Yue Li, I-Ming Hsing
Summary: This study comprehensively investigates the structural and functional properties of ion-embedded PEDOT:PSS modified by different annealing temperatures, and correlates them to the performance of the IGT. The results show that short-time high-temperature annealing treatment improves the film's conductivity and hole mobility, leading to enhanced performance of the IGT.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Analytical
Xinyu Zhuang, Zibin Zhao, Xianzhen Feng, Grace C. Y. Lui, Denise Chan, Shui Shan Lee, I-Ming Hsing
Summary: This study presents a pooling test platform that allows for sample specific detection of multiple samples in a single run. It eliminates the need for retesting and can effectively solve the bottleneck issues in diagnostic pooling testing.
ANALYTICAL CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Yue Li, Zibin Zhao, Asmita Veronica, Sin Yu Yeung, I-Ming Hsing
Summary: Continuous electrocardiography (ECG) monitoring is necessary for preventing cardiac events and premature death. This study introduces a 12-lead ECG patch that overcomes issues with compliance, skin damage, and user discomfort in conventional ECG sensors. The patch is safe, stretchable, and skin-adherent, allowing for user-friendly ECG monitoring.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Biophysics
Yumeng Cao, Xiao Lu, Haosi Lin, Alan Fernando Rodriguez Serrano, Grace C. Y. Lui, I-Ming Hsing
Summary: This study presents a CRISPR-based CoLAMP assay for rapid diagnosis of SARS-CoV-2, which can detect the viral RNA in clinical samples within 40 minutes with a sensitivity of 0.5 copies/μL.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Xianzhen Feng, Xinyu Zhuang, Grace Lui, I-Ming Hsing
Summary: The study introduces FLIPNAS, an efficient method for nucleic acid pooling detection, which can uniquely label and test all subsamples in a single-time detection among pools of samples. FLIPNAS can recognize one to eight positive subsamples without retesting positive pools, and it shows sufficient sensitivity for mass monitoring of SARS-CoV-2 without cross-reactivity against other respiratory diseases. This method can improve the efficiency of nucleic acid pooling detection, accelerate testing, and alleviate the urgent demand for resources.
Article
Nanoscience & Nanotechnology
Shiyao Wang, Ailin Qin, Li Yin Chau, Eunice W. T. Fok, Mei Yue Choy, Christopher J. Brackman, Gilman K. H. Siu, Chien-Ling Huang, Shea Ping Yip, Thomas M. H. Lee
Summary: In this study, CdSeS/ZnS quantum dots with surface modification of cysteamine were used as a new reporter probe for LAMP, enabling single-copy sensitivity. The positively charged amine-QDs remained dispersed in negative LAMP samples, while they became precipitated in positive LAMP samples. This method has the advantages of compatibility and universality.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Haosi Lin, Alan F. Rodriguez-Serrano, I-Ming Hsing
Summary: The study utilized aTF-based biosensors to achieve rapid detection of uric acid in non-invasive salivary samples, enhancing signal enrichment and overcoming the negative impact of matrix effect by engineering a two-pass TMSD circuit. The improvements halved the turnaround time and enabled semi-quantitative detection in normal salivary UA levels, showing the potential of aTF-based biosensors in metabolite detection for health monitoring.