Article
Agronomy
Qingxi Yang, Qian Zhou, Xin Zhou, Huixin Fang, Yingbo Zhao, Baodong Wei, Shujuan Ji
Summary: Postharvest yellowing is the main manifestation of rapid deterioration in broccoli, leading to a significant reduction in glucosinolates content. The reduction in glucoraphanin and sulforaphane content is mainly attributed to the continuous down-regulation of related genes.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Horticulture
Jung Su Jo, Shiva Ram Bhandari, Gwan Ho Kang, Yu Kyeong Shin, Jun Gu Lee
Summary: This study investigated the GSL profile, hydrolysate and nitrile contents, and nitrile formation rate of three commercial cultivars and eleven inbred broccoli lines. The results showed that glucobrassicin and glucoraphanin were the most dominant GSLs in all lines. Based on GSL content, five lines were selected as potential highly functional broccoli lines. Three lines exhibited relatively higher total hydrolysates. Three inbred lines with low nitrile formation rate and high isothiocyanate formation were selected as functional broccoli breeding lines. Therefore, hydrolysate and GSL content are deemed more reliable for selecting and developing highly functional varieties.
SCIENTIA HORTICULTURAE
(2022)
Article
Nutrition & Dietetics
John A. Bouranis, Laura M. Beaver, Jaewoo Choi, Carmen P. Wong, Duo Jiang, Thomas J. Sharpton, Jan F. Stevens, Emily Ho
Summary: Research indicates that the production of isothiocyanates may be influenced by the composition of the gut microbiome, potentially impacting the efficacy of interventions with cruciferous vegetables. In humans, there is significant inter-individual variability in SFN-NIT excretion following consumption of broccoli sprouts.
Article
Plant Sciences
Ryota Endo, Hiroshi Chikano, Etsuko Itabashi, Mitsuyo Kawasaki, Takayoshi Ohara, Tomohiro Kakizaki
Summary: Research has shown that glucosinolates (GSLs), which are present in Brassicaceae plants, can have health benefits. Raphanobrassica, an inter-generic hybrid between radish and kale, contains a high amount of sulforaphane (4MSOB), a functional component unique to cruciferous vegetables. This study found that Raphanobrassica with defective GRS1 can be bred to have a high 4MSOB content, making it a new leafy vegetable with potential anti-cancer and anti-inflammatory effects.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Nutrition & Dietetics
John A. Bouranis, Laura M. Beaver, Emily Ho
Summary: The gut microbiome plays a key role in influencing the metabolic fate and activity of isothiocyanates (ITCs) from cruciferous vegetables. Variations in gut microbiome composition among individuals can lead to differences in ITC absorption and excretion, impacting the potential use of these compounds in disease prevention and treatment. Understanding the interplay between gut microbiome and ITC formation through novel omics technologies is essential for optimizing the health benefits of phytochemicals.
FRONTIERS IN NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Junwei Wang, Shuxiang Mao, Mantian Liang, Wenxia Zhang, Fangzhen Chen, Ke Huang, Qiuyun Wu
Summary: This study found that exogenous methyl jasmonate (MeJA) treatment can promote glucosinolate (GSL) biosynthesis and sulforaphane accumulation in broccoli, with a more pronounced effect on florets. Additionally, MeJA treatment increases flavonoid content and antioxidant activity in broccoli florets.
Article
Plant Sciences
Jinyu Bao, Xu Lu, Lei Ma, Xiumin Zhang, Peng Tian, Xiaoling Zhang, Sheng Li, Shaoying Ma, Jie Yang, Yaqi Lu, Yunchun Wei, Congcong Zhang, Xiaotong Shi
Summary: Hairy roots of broccoli obtained through Agrobacterium rhizogenes infection have phytohormone autonomy and produce anti-cancer substances. Under the induction of methyl jasmonate, the production of the anti-cancer substances in broccoli hairy roots significantly increased. This study revealed the molecular mechanism of anti-cancer substance synthesis in broccoli hairy roots under methyl jasmonate treatment.
JOURNAL OF PLANT RESEARCH
(2022)
Article
Plant Sciences
Xiumin Zhang, Xu Lu, Shaoying Ma, Jinyu Bao, Xiaoling Zhang, Peng Tian, Jie Yang, Yaqi Lu, Sheng Li
Summary: The study demonstrated that MeJA treatment can increase the content of sulforaphane in broccoli hairy roots and decrease the activity of myrosinase. Transcriptome analysis revealed that the release of glucoraphanin and sulforaphane in hairy roots is associated with the ABC transporter gene family and SNARE protein-mediated exocytosis.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2021)
Article
Chemistry, Organic
Jun Zhou, Zhiyuan Bao, Panpan Wu, Chao Chen
Summary: Diaryliodonium salts derived from gemfibrozil and gemfibrozil methyl ester were synthesized with good regioselectivity and successfully utilized in various derivatization reactions.
SYNTHESIS-STUTTGART
(2022)
Article
Multidisciplinary Sciences
Dominik Kolodziejski, Izabela Koss-Mikolajczyk, Hansruedi Glatt, Agnieszka Bartoszek
Summary: The chemopreventive properties of Brassica vegetables are mainly attributed to their characteristic compounds, glucosinolates (GLs) and their degradation products, isothiocyanates (ITCs) and indoles. This study found that intact GLs did not show cytotoxic activity on cancer cells, but their conversion to ITCs or indoles by myrosinase led to significant inhibition of cancer cell growth. The pure ITC/indole solutions showed the strongest antiproliferative activity. GLs were found to cause DNA modification to a greater extent than ITCs, and metabolic activation increased this effect.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Medicinal
J. Jonathan Nue-Martinez, David Cisneros, Christophe Dardonville
Summary: The synthesis and use of a new reagent for the preparation of DNA minor groove binding molecules with antiprotozoal activity is reported. The reagent allows access to previously inaccessible compounds and can be purified using a two-step protocol. This methodology enables the synthesis of important antiparasitic compounds.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Organic
Subhash P. Chavan, Sanket A. Kawale, Niteen B. Patil, Dinesh B. Kalbhor
Summary: The formation of allyl amine from aziridine-2-alcohol was achieved through PPh3/I-2/imidazolde mediation for the synthesis of N-(tert-butoxycarbonyl)-D-vinyl glycine methyl ester.
TETRAHEDRON LETTERS
(2021)
Article
Multidisciplinary Sciences
Justyna Piechocka, Monika Wyszczelska-Rokiel, Rafal Glowacki
Summary: This report presents a method for simultaneous determination of plasma HPPTCA and total low molecular-weight thiols content. The assay is based on HPLC-UV, involving disulfides reduction, derivatization, and sample deproteinization. The HPLC-UV assay provides a complementary tool for routine clinical analysis and facilitates further studies on the role of aminothiols and HPPTCA in living systems.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Inorganic & Nuclear
T-H Liao, W-Y Hu, H. Sun, W-J Ye, Q. Guo, Z-X Zhou
Summary: The structures of two compounds were obtained through synthesis and spectroscopic confirmation, and were further analyzed by X-ray diffraction and DFT calculations. The experimental and theoretical results were consistent, and the molecular properties of these compounds were studied.
JOURNAL OF STRUCTURAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Vijaya C. Lakshmi, Naresh Kumar Katari, Giri M. Prasad, Nagaraju Kerru, Vijay Kumar Rekulapally, Sreekantha B. Jonnalagadda
Summary: An efficient protocol for synthesis of Erythro (+/-) and Threo (+/-) is described, with excellent yields achieved through simple reduction and inversion method. The method is cost-effective, high-yielding, and suitable for large-scale synthesis.
RUSSIAN JOURNAL OF GENERAL CHEMISTRY
(2021)
Article
Food Science & Technology
Carla Kuehn, Franziska Kupke, Susanne Baldermann, Rebecca Klopsch, Evelyn Lamy, Silke Hornemann, Andreas F. H. Pfeiffer, Monika Schreiner, Franziska S. Hanschen, Sascha Rohn
MOLECULAR NUTRITION & FOOD RESEARCH
(2018)
Article
Nutrition & Dietetics
Franziska S. Hanschen, Susanne Baldermann, Adrian Brobrowski, Andrea Maikath, Melanie Wiesner-Reinhold, Sascha Rohn, Monika Schreiner
Article
Chemistry, Applied
Rebecca Klopsch, Susanne Baldermann, Franziska S. Hanschen, Alexander Voss, Sascha Rohn, Monika Schreiner, Susanne Neugart
Review
Agronomy
Franziska S. Hanschen, Traud Winkelmann
Article
Food Science & Technology
Nicole S. Wermter, Sascha Rohn, Franziska S. Hanschen
Article
Food Science & Technology
Mareike Krell, Lina Cvancar, Michael Poloczek, Franziska S. Hanschen, Sascha Rohn
Summary: The study revealed that GLS and their breakdown products like ITC migrate into the surrounding food matrix during baking and react with proteins, altering protein properties and reducing the bioavailability of ITC and lysine.
Editorial Material
Food Science & Technology
Franziska S. Hanschen, Sascha Rohn
Article
Chemistry, Applied
Katharina Friedrich, Nicole S. Wermter, Lars Andernach, Katja Witzel, Franziska S. Hanschen
Summary: This study investigated the accumulation and formation of sulfur-containing compounds in Brassica vegetables. The results showed that SMCSO was the main sulfur compound, and it degraded to other compounds upon heating. The SMCSO content was similar in white and red cabbages. Proteome profiling revealed a correlation between the recovery of sulfur compounds and the abundance of two cystine lyase isoforms.
Article
Food Science & Technology
Holger Hoffmann, Lars Andernach, Clemens Kanzler, Franziska S. Hanschen
Summary: A novel type of glucosinolate degradation product was identified in this study, and its formation mechanism was proposed. Furthermore, the content of two isothiocyanate derivatives was quantified in boiled cabbage samples.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Mareike Krell, Franziska S. Hanschen, Sascha Rohn
Summary: This study aimed to evaluate the potential increase in isothiocyanates (ITC) formation in Brassicaceae-enriched bread while minimizing ITC-protein conjugates. The results showed that pH values of the bread influenced the degradation of glucosinolates (GLS), but there was no significant correlation between pH values and BITC content in model breads. The baking process significantly reduced the ITC content, but the degradation products and BITC-Lys conjugates were stable during storage in the bread matrix itself.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Nutrition & Dietetics
Holger Hoffmann, Christiane Ott, Jana Raupbach, Lars Andernach, Matthias Renz, Tilman Grune, Franziska S. Hanschen
Summary: Thermal glucosinolate degradation products, specifically 3-alk(en)yl-4-hydroxythiazolidine-2-thiones, were found to be formed during the boiling of cabbage and can be absorbed by the human body via passive diffusion. These compounds showed no acute cell toxicity and no significant changes in antioxidant potential or NAD(P)H dehydrogenase quinone 1 (NQO1) activity. However, they exhibited a certain degree of inhibition on glycogen synthase kinases-3 (GSK-3). This study did not find any health-promoting or toxic effects of these degradation products.
FRONTIERS IN NUTRITION
(2022)
Article
Food Science & Technology
Holger Hoffmann, Susanne Baldermann, Melanie Wiesner-Reinhold, Manuela M. Bergmann, Tilman Grune, Franziska S. Hanschen
Summary: This study investigated the bioavailability and metabolism of longer-chain epithionitriles in Brassica vegetables. Human participants consumed a beverage rich in specific epithionitriles and urine and blood samples were analyzed. The metabolites were identified and quantified using UHPLC-TOF-MS, and the standards for these metabolites were synthesized and confirmed. The results suggest different metabolism pathways for different epithionitriles.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Chemistry, Applied
Matthias Renz, Matthijs Dekker, Sascha Rohn, Franziska S. Hanschen
Summary: Brassica vegetables, rich in glucosinolates (GLSs), have health benefits due to the breakdown of GLSs into isothiocyanates. This study found that the heat stability and conversion of GLSs to nitriles in vegetable broths were influenced by vegetable type and plant matrix concentration. The redox status of the matrix, with H2S as an important factor, also played a role in these processes.
Article
Agriculture, Multidisciplinary
Vanda Pucikova, Sascha Rohn, Franziska S. Hanschen
Summary: Leaf age significantly affects the accumulation and hydrolysis of glucosinolates and their hydrolysis products in two leafy Brassica vegetables. Young leaves contain more than 3-fold more glucosinolates and release more isothiocyanates compared to older ones. Myrosinases hydrolyze all amounts of glucosinolates regardless of leaf age. Moreover, older leaves supply younger leaves with glucosinolates. Therefore, incorporating young leaves of leafy Brassicas in the diet can enhance the nutritional value of food.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Applied
Matthias Renz, Lars Andernach, Martin Kaufmann, Sascha Rohn, Franziska S. Hanschen
Summary: Red cabbage, a popular vegetable in Central Europe, contains glucosinolates (GLSs), which can be hydrolyzed to form health-promoting isothiocyanates (ITCs), nitriles, and epithionitriles. This study found that vinegar and baking soda significantly affected the stability of GLSs and the formation of nitriles during cooking of red cabbage. Primary amines were identified as the major degradation product of GLSs. Additionally, the formation of various N,N'-dialk(en)yl thioureas during boiling of Brassica vegetables was demonstrated for the first time.
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)