Article
Food Science & Technology
Ping-Hsiu Huang, Yu-Tsung Cheng, Hung-Chun Hsieh, Wen-Ching Ko, Wen-Chien Lu, Po-Hsien Li
Summary: This study investigated the effects of TGase on the quality of tilapia surimi and kamaboko under different thermal temperatures and pressures. TGase promoted the formation of gel and cross-linking. The optimal reaction temperature for TGase was 50°C. Both thermal and high-pressure treatments improved the rheological properties of the products. The protein solubility of surimi and kamaboko increased under thermal treatment and pressurization.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Lien Dejager, Mark Jairaj, Kieran Jones, Timothy Johnson, Sherri Dudal, Yves Dudal, Patrick Shahgaldian, Rita Correro, Jun Qu, Bo An, Richard Lucey, Szabolcs Szarka, Robert Wheller, Alina Pruna, Sarah Kettell, Andrew Pitt, Paul Cutler
Summary: Determining the levels of protein cross-linking catalysed by transglutaminase 2 in different disease states has been a difficult task. However, a new method using LC-MS/MS detection based on multiple reaction monitoring has been developed, allowing for efficient digestion without enzyme contamination and providing a sensitivity of 0.1 ng/mL of epsilon-(gamma-glutamyl) lysine in human urine with precision of less than 20% CV. This approach represents a significant advance in the detection and quantification of epsilon-(gamma-glutamyl) lysine.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Editorial Material
Cell Biology
Karolina Pircs, Roger A. Barker, Johan Jakobsson
Summary: Huntington disease is a genetic neurodegenerative disorder that affects cells in the brain, with the underlying mechanism still unresolved. Recent research suggests that dysfunction in macroautophagic/autophagic pathways may contribute to cellular dysfunction and death.
Article
Biochemistry & Molecular Biology
Ann-Kathrin Weishaupt, Laura Kubens, Lysann Ruecker, Tanja Schwerdtle, Michael Aschner, Julia Bornhorst
Summary: Neurotransmitters like dopamine, serotonin, GABA, and acetylcholine have a crucial role in transmitting excitation between neurons in the central nervous system. Imbalance in neurotransmitter levels is associated with neurological disorders, making it important to accurately assess and target neurotransmitter levels. The model organism C. elegans, along with a validated LC-MS/MS method, provides a powerful tool for studying neurotoxicity mechanisms and identifying potential therapies.
Review
Biochemistry & Molecular Biology
Besnik Muqaku, Patrick Oeckl
Summary: Mass spectrometry is an important technique for analyzing biomolecules, especially in the field of neurodegenerative diseases where peptidomics analysis of cerebrospinal fluid samples has played a crucial role.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Cosima Damiana Calvano, Ilario Losito, Tommaso Cataldi
Summary: This special issue focuses on the contribution of dysregulation of lipid signaling and metabolism to neurodegenerative diseases, including Alzheimer's and Parkinson's, summarizing three reviews and one research article.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Yulemni Morel, Nivedita Hegdekar, Chinmoy Sarkar, Marta M. Lipinski, Maureen A. Kane, Jace W. Jones
Summary: The study developed a targeted, quantitative method using HILIC separation and selective reaction monitoring mass spectrometry for specific and sensitive quantitation of 100 PE-P structures. Application of this assay to a TBI mouse model demonstrated distinct temporal profiles for plasma PE-P up to 28 days post injury, showing a significant increase 24 hours after induced TBI followed by a gradual reduction to sham concentrations by day 28.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Qin Chen, Ying Li, Lezhen Dong, Ranran Shi, Zhen Wu, Lianliang Liu, Jingshun Zhang, Zufang Wu, Daodong Pan
Summary: This study developed a sensitive and easy method to directly analyze CML by UPLC-MS/MS. The CML without derivatization could be well retained and separated after optimizing the pretreatment conditions. Oasis MCX SPE cartridge showed the best recovery rate and purification ability for CML. This method is suitable for quantifying CML content in sterilized milk with high recovery rate and low detection and quantification limits.
Article
Agriculture, Multidisciplinary
Sarah Schlag, Soren Goetz, Felix Ruettler, Sandra M. Schmoeckel, Walter Vetter
Summary: This study analyzed phytosterols in 34 different quinoa accessions and identified multiple sterols using gas chromatography with mass spectrometry. A novel internal standard was used for quantification and minor sterols were determined after enrichment. The total sterol content in quinoa was found to be higher than previously reported, with & UDelta;7-sterols contributing the most. Cluster analysis grouped the accessions into four distinct groups based on different sterol patterns.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Artem Artiukhov, Ekaterina F. Kolesanova, Aleksandra Boyko, Anastasiya A. Chashnikova, Sergei N. Gnedoy, Thilo Kaehne, Daria A. Ivanova, Alyona Kolesnichenko, Vasily A. Aleshin, Victoria Bunik
Summary: The study focuses on the glutarylation of lysine residues in proteins, revealing sample-specific patterns of protein glutarylation in rat tissues and mitochondria. Time-dependent fragmentation of glutarylated proteins in rat tissue homogenates was observed, complicating the investigation of acylation regulators' effects. The affinity-purified anti(epsilon-glutaryllysine)-antibodies proved to be an effective tool for characterizing protein glutarylation and showing its specific pattern compared to other acylation types in complex protein mixtures.
Review
Chemistry, Analytical
Xin Wang, Wenxiu Zhou, Zhenye Gao, Xiaoyuan Lv
Summary: This review summarizes the application of mass spectrometry techniques in the analysis of protein S-nitrosylation, including sample preparation techniques and qualitative and quantitative strategies. The roles of protein S-nitrosylation in human diseases are discussed, with an emphasis on current limitations and future research directions.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Alyssa S. Aebersold, Akhilesh Kumar, Zhao-Hui Song
Summary: A method using liquid chromatography-tandem mass spectrometry was developed to quantify the concentration of cannabidiol (CBD) in aqueous humor. The study found that the concentration of CBD in the aqueous humor of mice reached a maximum of 71.55 ng/mL, with a peak time of 2 hours and a half-life of 1.046 hours. This method is important for assessing the aqueous humor concentrations of CBD and its ocular pharmacologic effects.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Katleen Van Uytfanghe, Maria del Mar Ramirez Fernandez, Aurelie De Vos, Sarah M. R. Wille, Christophe Pol Stove
Summary: This study developed a new method for PEth measurements and achieved good comparison results through the collaboration of two laboratories. The research demonstrates that the method has good comparability and reproducibility, allowing for accurate cross-validation between laboratory networks.
Article
Chemistry, Analytical
Armen Shamirian, Christopher Foti, Scott S. Mitchell
Summary: This study developed a sensitive and selective method for simultaneous determination of B(2)Pin(2) and B-2(OH)(4), with B-2(OH)(4) derivatized before analysis by GC-MS and B(2)Pin(2) analyzed without derivatization. The method has low detection and quantitation limits, good recoveries and linear response for analytes at different levels in drug substances.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Ghadeer A. Elbaz, Hala E. Zaazaa, Lobna M. Abd El Halim, Hany H. Monir
Summary: This study developed a new, green, and sensitive analytical method for quantifying pesticide residues in thyme and guava leaves. The method involved using the QuEChERS protocol for extraction and analysis using liquid chromatography triple quadrupole mass spectrometry. The newly developed method showed good performance in terms of linear response, wide concentration range, and low limits of detection for both pesticides. It was also found to be more eco-friendly compared to reported methods.
MICROCHEMICAL JOURNAL
(2023)
Article
Pharmacology & Pharmacy
Martin Dulac, Amor Sassi, Citra Nagarathinan, Marie-Odile Christen, Patrick M. Dansette, Daniel Mansuy, Jean-Luc Boucher
DRUG METABOLISM AND DISPOSITION
(2018)
Article
Pharmacology & Pharmacy
Martin Dulac, Citra Nagarathinam, Patrick Dansette, Daniel Mansuy, Jean-Luc Boucher
DRUG METABOLISM AND DISPOSITION
(2019)
Article
Anatomy & Morphology
Irina Rakotoson, Brigitte Delhomme, Philippe Djian, Andreas Deeg, Maia Brunstein, Christian Seebacher, Rainer Uhl, Clement Ricard, Martin Oheim
FRONTIERS IN NEUROANATOMY
(2019)
Article
Biochemistry & Molecular Biology
Alejandro Orrico-Sanchez, Laetitia Chausset-Boissarie, Rodolphe Alves de Sousa, Basile Coutens, Sara Rezai Amin, Vincent Vialou, Franck Louis, Assia Hessani, Patrick M. Dansette, Teodoro Zornoza, Carole Gruszczynski, Bruno Giros, Bruno P. Guiard, Francine Acher, Nicolas Pietrancosta, Sophie Gautron
MOLECULAR PSYCHIATRY
(2020)
Article
Multidisciplinary Sciences
Guillaume Van der Rest, Human Rezaei, Frederic Halgandi
SCIENTIFIC REPORTS
(2019)
Article
Biochemical Research Methods
Corentin Berardet, Julia Kaffy, Frederic Halgand, Guillaume van der Rest, Sandrine Ongeri, Myriam Taverna
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Medicinal
Lilian Dubois, Nicolas Pietrancosta, Alexandre Cabaye, Isabelle Fanget, Cecile Debacker, Pierre-Andre Gilormini, Patrick M. Dansette, Julien Dairou, Christophe Biot, Roseline Froissart, Anne Goupil-Lamy, Hugues-Olivier Bertrand, Francine C. Acher, Isabelle McCort-Tranchepain, Bruno Gasnier, Christine Anne
JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Victor Le-Minh, Frederic Halgand, Guillaume Van der Rest, Myriam Taverna, Claire Smadja
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2020)
Article
Biochemistry & Molecular Biology
Jan Bohl, Cecile Sicard, Human Rezaei, Guillaume Van der Rest, Frederic Halgand
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2020)
Article
Chemistry, Physical
Marwa Abdelmouleh, Mathieu Lalande, Edith Nicol, Gilles Frison, Guillaume van der Rest, Jean-Christophe Poully
Summary: It has been shown that 18-crown-6-ether plays a more active role in chemical processes during activation in mass spectrometers, abstracting protons or protonated molecules from peptides and potentially changing binding sites. Experimental and theoretical results support this new perspective.
Review
Biochemistry & Molecular Biology
Derek R. Boyd, Narain D. Sharma, Paul J. Stevenson, Patrick Hoering, Christopher C. R. Allen, Patrick M. Dansette
Summary: Enzymatic oxidations of thiophenes play a crucial role in biodesulfurization of crude oil and drug metabolism. The structure and configuration of the metabolites obtained from thiophene enzymatic oxidation depend on the substrate and selected oxygenase, and have significant implications in the pharmaceutical industry and medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Anouchka Gatin, Patricia Duchambon, Guillaume van der Rest, Isabelle Billault, Cecile Sicard-Roselli
Summary: This study investigates the dimerization behavior of tyrosine when embedded in a peptidic sequence. The results show that the formation of tyrosine dimers is a slow process and involves the co-existence of intermediates and final products. The involvement of amide functions in protein dimerization is also suggested.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Yong Wang, Pascal Pigeon, Wei Li, Jiangkun Yan, Patrick M. Dansette, Mohamed Othman, Michael J. McGlinchey, Gerard Jaouen
Summary: Ferrociphenols, compounds characterized by a biologically active redox motif, have anticancer properties. This study synthesized a variety of ferrociphenols with nitrogen-containing substituents and investigated their structure-activity relationships. Oxidative metabolism experiments and ROS-related bioactivity measurements were conducted to explore the cytotoxicity of imidoferrociphenols. Additionally, dimerization phenomenon observed in the crystal structure of a ferrociphenol may affect its antitumor activity.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Isabel De Figueiredo, Bernard Bartenlian, Guillaume van der Rest, Antoine Pallandre, Frederic Halgand
Summary: Protein biomarkers are important for disease diagnostics and monitoring, but their detection is challenging due to the complex proteome in biological samples. Microfluidics coupled with mass spectrometry has emerged as a cutting-edge approach for early pathology detection.
Article
Biochemistry & Molecular Biology
Anouchka Gatin, Isabelle Billault, Patricia Duchambon, Guillaume Van der Rest, Cecile Sicard-Roselli
Summary: This study investigated the formation of di-tyrosine bridges in different biological compounds through gamma radiolysis and chromatographic-mass spectrometry analysis, revealing the presence of multiple di-tyrosine isomers and raising questions about their respective roles and toxicity in vivo. A better understanding of the type of di-tyrosine detected in vivo is now necessary, especially considering its common use as a biomarker.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
