Article
Pharmacology & Pharmacy
Moushimi Amaya, Han Cheng, Viktoriya Borisevich, Chanakha K. Navaratnarajah, Roberto Cattaneo, Laura Cooper, Terry W. Moore, Irina N. Gaisina, Thomas W. Geisbert, Lijun Rong, Christopher C. Broder
Summary: The study developed a high-throughput screening platform based on a recombinant version of Cedar virus to accelerate the development of anti-henipavirus drugs. Through screening and validation of compounds, potential compounds with potent antiviral activity were identified.
ANTIVIRAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Parisa Shiri, Veronica Lai, Tara Zepel, Daniel Griffin, Jonathan Reifman, Sean Clark, Shad Grunert, Lars P. E. Yunker, Sebastian Steiner, Henry Situ, Fan Yang, Paloma L. Prieto, Jason E. Hein
Summary: Solubility screening is essential but labor intensive. By developing a flexible robotic system with computer vision and iterative feedback, researchers can successfully measure solubility in multiple solvents autonomously, saving time and resources.
Review
Biochemical Research Methods
Lihao Fu, Erpeng Guo, Jianzhi Zhang, Keyi Li, Yongcan Chen, Tong Si
Summary: Microbial cell factories play a crucial role in converting renewable feedstocks into chemicals and materials. The lack of predictive modeling has made high-throughput screening essential for microbial strain engineering. Mass spectrometry (MS) has emerged as a powerful tool for label-free detection, offering high sensitivity and chemical specificity. Recent advancements in rapid MS assays have significantly improved screening efficiency for microbial libraries, approaching a rate of one sample per second. However, unique challenges in MS screening of strain libraries need to be addressed for future solutions.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Melissa S. Love, Case W. McNamara
Summary: Recent advancements in drug discovery efforts for cryptosporidiosis have been inspired by the challenges posed by the unique biology of Cryptosporidium. Phenotypic-based screens and repositioning of hits from other pathogens have created a pipeline of potential therapeutics for this disease. Improved methodologies in phenotypic assays are facilitating the development of new compounds for clinical testing.
EXPERT OPINION ON DRUG DISCOVERY
(2021)
Article
Physiology
Satish Kantipudi, Daniel Harder, Dimitrios Fotiadis
Summary: Amino acids are crucial for cellular functions and their translocation across biological membranes is mediated by amino acid transporters. 4F2hc-LAT1 is an important amino acid transporter involved in tumor progression and is considered a potential anticancer target. This study used a radioligand binding assay called SPA to identify and characterize ligands of the 4F2hc-LAT1 transporter. The binding affinities determined by SPA were comparable to those from cell-based assays, but with higher reliability due to the use of purified protein.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biophysics
Min Li, Huijuan Yu, Yiran Li, Xin Li, Shiqing Huang, Xiaogang Liu, Gaoqi Weng, Lei Xu, Tingjun Hou, Dong-Sheng Guo, Yi Wang
Summary: By utilizing supramolecular chemistry and artificial peptide library, a turn-on fluorescent sensing system for intracellular imaging of histone deacetylase 1 (HDAC1) was developed, allowing specific and highly sensitive detection, and a novel HDAC1 down-regulator was identified through high-throughput screening.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Medicinal
Zhongyuan Luo, Daohai Du, Yanjun Liu, Tian Lu, Liping Liu, Hualiang Jiang, Kaixian Chen, Changliang Shan, Cheng Luo
Summary: A new high-throughput screening method was developed to discover G6PD inhibitors, with Wedelolactone identified as a strong inhibitor in a non-competitive, reversible manner, as confirmed by surface Plasmon Resonance assay. The study also showed the inhibitory effect of Wedelolactone on ovarian cancer cell proliferation through targeting G6PD.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Katja F. Hellendahl, Maryke Fehlau, Sebastian Hans, Peter Neubauer, Anke Kurreck
Summary: The study introduces a luciferase-based assay for the detection of NK activity, which was validated in a 96-well plate format. The high-throughput assay showed comparable results to HPLC analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Zhichao Yu, Hexiang Gong, Fangqin Xue, Yongyi Zeng, Xiaolong Liu, Dianping Tang
Summary: In this study, a flexible and low-cost thermosensitive fiber paper was developed for visual display in photothermal biosensing systems for early acute myocardial infarction. The color change mechanism of the thermal paper was based on the temperature-driven reversible transformation of the dye molecule. The results provide new insights for the development of low-cost, instrument-free portable photothermal biosensors.
ANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Yihui Song, Min Zhao, Yahong Wu, Bin Yu, Hong-Min Liu
Summary: A high-throughput screening protocol was developed to discover SHP2 inhibitors, resulting in the identification of new SHP2-PTP inhibitors and allosteric inhibitors. These inhibitors showed potential for targeting cancer-associated SHP2 mutations.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Biochemistry & Molecular Biology
Jacob F. Wardman, Lyann Sim, Jennifer Liu, Teresa A. Howard, Andreas Geissner, Phillip M. Danby, Alisdair B. Boraston, Warren W. Wakarchuk, Stephen G. Withers
Summary: Mucin-type O-glycosylation is an important post-translational modification involved in cellular communication. However, the study of O-glycoproteins and O-glycans is challenging due to the lack of available enzymes for their assembly and degradation. To overcome this, a genetically encoded screening method was developed using engineered Escherichia coli to screen for enzymes that act on O-glycoproteins. The method uses an O-glycosylated fluorescence resonance energy transfer probe as a read-out of glycosylation state and can also assay glycosidases and glycosyltransferases. The methodology has potential for the directed evolution of O-glycopeptidases.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Immunology
Xiaojing Wen, Li Zhang, Shan Zhao, Qiang Liu, Wenyi Guan, Jiajing Wu, Qiwei Zhang, Hongling Wen, Weijin Huang
Summary: The study found that cardamomin has good anti-human adenovirus 5 (HAdV5) activity both in vitro and in vivo, and can protect tissues from virus-induced damage. This research established a method for high-throughput screening of anti-HAdV5 drugs and demonstrated cardamomin as a potential new treatment for patients infected with adenoviruses.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biology
Hang Ao, Weiwei Chen, Jie Wu, Wencheng Xiao, Huangxian Ju
Summary: In this study, a chemiluminescent screening method was developed for fast screening of specific hybridoma cells. The method utilizes a proximity-rolling circle activated enzymatic switch strategy on a conventional 96-well plate. This protocol enables efficient screening without the need for tedious cloning process, greatly improving the efficiency of hybridoma technology.
COMMUNICATIONS BIOLOGY
(2022)
Article
Chemistry, Analytical
So-Young Lee, Jae-Cheong Lim, Eun-Ha Cho, Sung-Hee Jung
Summary: CD55 expression levels are significantly higher in metastatic tumor tissues compared to serum in early stages of pleural metastatic lung cancer. However, the concentration of CD55 in serum decreases as metastasis progresses, indicating its potential as a biomarker for predicting lung cancer metastasis.
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
(2021)
Review
Pharmacology & Pharmacy
Tuan Xu, Wei Zheng, Ruili Huang
Summary: In response to the COVID-19 pandemic, a panel of assays has been developed to screen drugs for anti-SARS-CoV-2 activity. The study found that multitarget assays were more accurate and efficient, while target-specific assays were better for investigating compound mechanisms of action. Additionally, strict filtering with counter screens may be more harmful than beneficial in identifying true positives, highlighting the importance of developing novel HTS assays against multiple targets in the SARS-CoV-2 life cycle.
DRUG DISCOVERY TODAY
(2021)
Article
Biochemistry & Molecular Biology
Egor S. Zhilin, Nadezhda E. Ustyuzhanina, Leonid L. Fershtat, Nikolay E. Nifantiev, Nina N. Makhova
Summary: The newly synthesized (1,2,5-oxadiazolyl)azasydnones showed excellent antiplatelet activity and selective inhibition of platelet aggregation mediated by ADP and adrenaline, while being completely non-toxic to the hybrid endothelial cells EaHy 926. This makes them promising drug candidates with improved antiplatelet profile and reduced toxicity for potential use in medicinal chemistry and drug design.
CHEMICAL BIOLOGY & DRUG DESIGN
(2022)
Article
Biochemistry & Molecular Biology
Olga N. Yudina, Marina L. Gening, Pinaki Talukdar, Alexey G. Gerbst, Yury E. Tsvetkov, Nikolay E. Nifantiev
Summary: This paper describes the synthesis of a linear tetramer and subsequent formation of a cyclic tetrasaccharide with axial amino groups starting from 3-azido-3-deoxy-1,2:5,6-di-O-isopropylidene-alpha-D-allofuranose. This cyclic tetramer serves as a potential scaffold for the synthesis of tetravalent functional clusters.
CARBOHYDRATE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Ekaterina D. Kazakova, Dmitry V. Yashunsky, Nikolay E. Nifantiev
Summary: The synthesis of the spacered A trisaccharide was achieved by employing a glycosyl donor with stereocontrolling groups, resulting in efficient and stereoselective formation of the required alpha-glycosylation product. The final products were deprotected and subjected to spacer biotinylation for use in biochemical studies.
Article
Biochemistry & Molecular Biology
Elena V. Sukhova, Dmitry V. Yashunsky, Ekaterina A. Kurbatova, Elina A. Akhmatova, Yury E. Tsvetkov, Nikolay E. Nifantiev
Summary: The synthetic pseudotetrasaccharides corresponding to repeating units of capsular polysaccharides of Streptococcus pneumoniae serotype 6A and 6B have been successfully created, showing immunogenic properties and the ability to mimic the corresponding polysaccharides. Immunological evaluation demonstrated specific epitopes recognized by anti-serogroup 6 antibodies.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Medicinal
Nadezhda E. Ustyuzhanina, Natalia Yu. Anisimova, Maria I. Bilan, Fedor V. Donenko, Galina E. Morozevich, Dmitriy V. Yashunskiy, Anatolii I. Usov, Nara G. Siminyan, Kirill I. Kirgisov, Svetlana R. Varfolomeeva, Mikhail V. Kiselevskiy, Nikolay E. Nifantiev
Summary: Polysaccharides extracted from fish and sea cucumber were found to stimulate bone marrow release of blood cells and have a significant effect on immunosuppressed mice, promoting proliferative activity of hematopoietic cells and restoration of spleen function. Further research on oligosaccharides as potential drugs for prophylaxis and therapy of hematopoiesis inhibition post chemotherapy and transplantation is warranted.
Review
Biochemistry & Molecular Biology
Mikhail Kiselevskiy, Natalia Yu Anisimova, Nadezhda E. Ustyuzhanina, Dmitry Z. Vinnitskiy, Alexandra Tokatly, Vera V. Reshetnikova, Irina O. Chikileva, Irina Zh Shubina, Kirill Kirgizov, Nikolay E. Nifantiev
Summary: Fucoidans, natural sulfated polysaccharides, have potential antitumor and anti-inflammatory properties. Although current experiments have shown low antitumor activity, there is potential for their application in supportive care and symptomatic therapy for cancer patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Chemistry, Medicinal
Anatolii I. Usov, Maria I. Bilan, Nadezhda E. Ustyuzhanina, Nikolay E. Nifantiev
Summary: Sulfated polysaccharides known as fucoidans, obtained from brown algae, have attracted attention due to their biological activities. Fucus vesiculosus and Ascophyllum nodosum, both belonging to Fucales order, are popular sources of commercial fucoidans. Despite their similarities in chemical composition and biological actions, these fucoidans differ considerably in their polysaccharide mixtures. Carefully characterized structures from both fucoidans may have valuable applications in drug development.
Review
Chemistry, Medicinal
Irina Kostareva, Kirill Kirgizov, Elena Machneva, Nadezhda Ustyuzhanina, Nikolay Nifantiev, Yulia Skvortsova, Irina Shubina, Vera Reshetnikova, Timur Valiev, Svetlana Varfolomeeva, Mikhail Kiselevskiy
Summary: Despite progress in allogeneic hematopoietic stem cell transplantation, chronic graft-versus-host disease remains a major complication. The lack of effective therapies and understanding of the disease mechanism highlights the importance of developing new treatments. Improved understanding of chronic GVHD mechanisms and advancements in diagnostic recommendations and medical tests have contributed to the search for effective drugs and medical studies. There is hope for successful treatment of this complication.
Article
Materials Science, Composites
Dmitry Z. Vinnitskiy, Anna L. Luss, Vadim B. Krylov, Nadezhda E. Ustyuzhanina, Anastasiya Goryachaya, Anna M. Nechaeva, Mikhail Shtilman, Nikolay E. Nifantiev, Yaroslav O. Mezhuev
Summary: A method was developed to conjugate aminopropyl spacer-bearing carbohydrates with epoxy groups on the surface of nanoparticles, opening up new possibilities for the design of nanocomposite drug forms. The effects of carbohydrate immobilization were studied by synthesizing a conjugate of the copolymer and a synthetic blood group antigen, and the composition and molecular weight of the hybrid nanoparticles were determined. These findings provide important insights for further research on polymer-carbohydrate ligand systems.
JOURNAL OF COMPOSITES SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Anna A. Grinkova, Elena V. Sukhova, Nadezhda E. Ustyuzhanina, Nikolay E. Nifantiev
Summary: In this study, a series of hyaluronic acid-related sugar derivatives were synthesized, and it was shown through in vitro experiments that tetra- and hexasaccharides have activity in inhibiting angiogenesis.
CARBOHYDRATE RESEARCH
(2022)
Article
Chemistry, Medicinal
Maria I. Bilan, Natalia Yu. Anisimova, Alexandra I. Tokatly, Sofya P. Nikogosova, Dmitriy Z. Vinnitskiy, Nadezhda E. Ustyuzhanina, Andrey S. Dmitrenok, Evgenia A. Tsvetkova, Mikhail V. Kiselevskiy, Nikolay E. Nifantiev, Anatolii I. Usov
Summary: Crude anionic polysaccharides from Lethasterias fusca were purified and analyzed. The main fraction LF was identified as having a dermatan core structure and the major component as dermatan sulfate LF-Derm. Unusual sulfation patterns were observed in the polysaccharides. LF-Derm and LF-Hep were found to stimulate hematopoiesis in vitro, suggesting that high sulfation may not be necessary for hematopoiesis stimulation in this case.
Article
Biochemistry & Molecular Biology
Anastasia A. A. Kamneva, Dmitry V. V. Yashunsky, Elena A. A. Khatuntseva, Nikolay E. E. Nifantiev
Summary: Spacer-armed pseudodi-, pseudotetra-, and pseudohexasaccharides related to the capsular phosphoglycan of Haemophilus influenzae type a were synthesized using iterative chain elongation. These compounds were used for designing neoglycoconjugates, such as a biotinylated glycoconjugate suitable for immunological studies.
Article
Biochemistry & Molecular Biology
Irina O. Chikileva, Alexandra V. Bruter, Nadezhda A. Persiyantseva, Maria A. Zamkova, Raimonda Ya. Vlasenko, Yuliya I. Dolzhikova, Irina Zh. Shubina, Fedor V. Donenko, Olga V. Lebedinskaya, Darina V. Sokolova, Vadim S. Pokrovsky, Polina O. Fedorova, Nadezhda E. Ustyuzhanina, Natalia Yu. Anisimova, Nikolay E. Nifantiev, Mikhail V. Kiselevskiy
Summary: This study aimed to develop a safer HER2-targeting CAR therapy by delivering CAR constructs into T and NK cells using a non-viral transfection method. The generated CAR-T-NK cells showed potent anti-tumor activity, suggesting transient expression of anti-HER2 CARs as a safer treatment for HER2-positive human cancers.
Article
Chemistry, Organic
Arsenii S. Solovev, Evgeniya M. Denisova, Ekaterina A. Kurbatova, Olga Y. Kutsevalova, Liubov G. Boronina, Vladimir A. Ageevets, Sergey V. Sidorenko, Vadim B. Krylov, Nikolay E. Nifantiev
Summary: Methylphosphorylated oligosaccharides structurally related to the O-antigens of Klebsiella pneumoniae were synthesized and used for antibody detection in ELISA. The antibodies specifically recognized the synthesized oligosaccharides, suggesting their potential use for serological diagnostics and strain serotyping of Klebsiella pneumoniae.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
A. Tokatly, D. Z. Vinnitskiy, N. E. Ustuzhanina, N. E. Nifantiev
Summary: Synthetic oligosaccharides are key model compounds for studying biological activity and important components for new drugs and glycoconjugate vaccines. This review discusses methods of stereocontrol in glycosylation reactions, including the use of protecting groups, deactivation, and intramolecular aglycone delivery. Various mechanisms for stereocontrol are explored, from neighboring group participation to remote participation from different positions on the glycosyl donor.
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
(2021)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)