Review
Biochemistry & Molecular Biology
Jenny Leopold, Patricia Prabutzki, Kathrin M. Engel, Juergen Schiller
Summary: In this update on the progress in MALDI-based lipid analysis since 2018, the selection of an appropriate matrix is found to be crucial for optimum results. The complexity of lipid classes increases from free fatty acids to cardiolipins and phosphoinositides over the last five years.
Article
Environmental Sciences
Xin Zhang, Yuxue Kong, Juan Cao, Hong Li, Rui Gao, Yujie Zhang, Kexin Wang, Yunfeng Li, Yanqin Ren, Wenxing Wang
Summary: An analytical method for determining atmospheric carbonyls and its application in the urban area of Beijing was developed in this study. The method provided important concentration information for studying chemical reaction processes and health effects.
Article
Chemistry, Analytical
Kenji Nakayama, Xin Li, Koji Shimizu, Shusuke Akamatsu, Takahiro Inoue, Takashi Kobayashi, Osamu Ogawa, Takayuki Goto
Summary: This study validates the feasibility of normalizing the entire phospholipid species in a complex biolipid background using only two phospholipid internal standards. Compared to traditional methods, the phospholipid ion normalization method provides better linear regression and higher measurement precision. Hence, a cost-effective, fast, simple, convenient, and reliable quantitative method, called qShot MALDI analysis, is proposed for the analysis of phospholipids that could be applied in clinical biomarker screening.
Article
Polymer Science
Zhiyuan Meng, Jie Yin, Fei Zhao, Mingxin Li, Yan Zhang, Yueyin Liang, Zhonglong Wang, Yiqin Yang
Summary: A new fluorescent probe CTS-NA-OAc was developed for detecting DNPH in environmental samples, showing highly specific response and low detection limit, suitable for DNPH detection in complex environments.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Engineering, Environmental
Christopher P. Alaimo, Yin Li, Peter G. Green, Michael J. Kleeman, Thomas M. Young
Summary: Airborne carbonyl compounds such as formaldehyde, acrolein, and methyl ethyl ketone have long been chemicals-of-concern in the environment due to their reactivity and their potential for negative health effects. The standard methods for determining carbonyls in air underreported both the total carbonyl load and the diversity of carbonyl species in natural gas and biogas samples, highlighting the need for improved analytical methods.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Romain Ladouce, Guillaume Fabien Combes, Katarina Trajkovic, Irena Drmic Hofman, Mladen Mercep
Summary: Oxidative stress and oxidative protein damage commonly occur in biological processes and diseases. The carbonyl group on amino acid side chains is a widely used biomarker for protein oxidation. However, the current DNPH immunoblotting method has limitations in standardization, technical bias, and reliability. To address these issues, a new blotting method using a biotin-aminooxy probe and p-phenylenediamine catalyst for carbonyl group derivatization under neutral pH conditions has been developed. This method improves the sensitivity and robustness of protein carbonyl detection and is compatible with protein immunoprecipitation.
Article
Biochemical Research Methods
Polina K. Vinel, S. Grobovoy, A. Sinitskii, O. L. Kolesnikov
Summary: The study aimed to modify a spectrophotometric assay for MAO activity evaluation with 2,4-dinitrophenylhydrazine, allowing accurate measurement of specific enzyme activity using different substrates.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Agricultural Engineering
Juana Rozalen, M. Mercedes Garcia, Amaya Zalacain, Horacio Lopez-Corcoles, Jorge Hurtado de Mendoza, Katrina Cornish, Manuel Carmona
Summary: Guayule, as a promising renewable source of natural rubber/latex, has resins with various activities potentially related to sesquiterpenes. By exploring different analytical methods, it was found that there may be new members of the guayulins family and the relationship in seasonal changes was identified.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Kazuaki Yoshinaga, Yuka Usami, Aya Yoshinaga-Kiriake, Hitomi Shikano, Shu Taira, Reiko Nagasaka, Seiya Tanaka, Naohiro Gotoh
Summary: The study on zebrafish models found that dietary DHA is initially structured into PC as a component of intestinal cell membranes, then gradually migrates to peripheral tissues such as muscles. These processes were observed and tracked in zebrafish at different time points.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Mariona Jove, Natalia Mota-Martorell, Elia Obis, Joaquim Sol, Meritxell Martin-Gari, Isidre Ferrer, Manuel Portero-Otin, Reinald Pamplona
Summary: The brain is one of the richest tissues in terms of lipid content and diversity in the human body, making it highly susceptible to oxidative stress. Glycerophospholipids, especially ether lipids, play a crucial role in the composition and functionality of the human brain. This article explores the involvement of ether lipids in the pathogenesis of sporadic Alzheimer's disease (sAD) and discusses their implications in neural cell physiology and antioxidant defense mechanisms.
Article
Biochemical Research Methods
Si -Yu Wang, Hui Liu, Jin-Hao Zhu, Shan -Shan Zhou, Jin-Di Xu, Jing Zhou, Qian Mao, Ming Kong, Song-Lin Li, He Zhu
Summary: In this study, a strategy based on DNPH capturing combined with MDF was established and validated to identify aliphatic aldehydes in biological samples. The strategy proved to be reliable in characterizing aliphatic aldehydes in biological samples and could be helpful in evaluating the physical conditions of subjects.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Chemistry, Analytical
M. Sejalon-Cipolla, P. Bruyat, S. Bregant, C. Malgorn, L. Devel, G. Subra, S. Cantel
Summary: MALDI-MS is crucial for detecting and identifying molecules involved in biological pathways, but many biomarkers remain undetectable due to various limitations. To overcome these challenges, labeling strategies and activity-based probes have been developed. Matrix-free derivatization techniques are currently gaining attention.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Madhuri Jayathirtha, Emmalyn J. Dupree, Zaen Manzoor, Brianna Larose, Zach Sechrist, Anca-Narcisa Neagu, Brindusa Alina Petre, Costel C. Darie
Summary: The human genome consists of around 30,000 genes, making humans slightly more complex than worms or flies. However, the complexity of humans mainly comes from the diverse proteins encoded by these genes, as well as the production of multiple proteins through alternative splicing and tissue-dependent expression. Post-translational modifications in proteins significantly increase the number of gene products or protein isoforms.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2021)
Article
Chemistry, Analytical
Holly-May Lewis, Priyanka Gupta, Kyle D. G. Saunders, Shazneil Briones, Johanna von Gerichten, Paul A. Townsend, Eirini Velliou, Dany J. V. Beste, Olivier Cexus, Roger Webb, Melanie J. Bailey
Summary: This work presents a new approach for measuring drug levels and lipid fingerprints in individual living mammalian cells. The method combines nanocapillary sampling with liquid chromatography to improve precision in drug analysis. By optimizing the transfer of analytes from the sampling capillary, the researchers achieved efficient measurement of drug analytes in 30 living cells. They also discovered cell-to-cell heterogeneity in drug molecule uptake and identified lipid features associated with bedaquiline uptake.
Article
Agriculture, Multidisciplinary
Chizumi Abe, Ye Zhang, Kazuhiro Takao, Kuni Sasaki, Koji Ochiai, Toshiro Matsui
Summary: This study visually investigated the dynamic production of soybean phytoalexins glyceollins in germinating soybeans inoculated with Aspergillus oryzae using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging. The results showed that glyceollins were only produced in the seed coat and germinated root regions of soybeans, and inoculation of inactivated fungi also promoted glyceollin production, suggesting a possible association with the fungus body.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Immunology
Martin Schulze, Gabor A. Czirjak, Karin Mueller, Ralf Bortfeldt, Markus Jung, Ulrike Jakop
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2019)
Article
Agriculture, Dairy & Animal Science
U. Jakop, V. Svetlichnyy, J. Schiller, M. Schulze, F. Schroeter, K. Mueller
ANIMAL REPRODUCTION SCIENCE
(2019)
Article
Biotechnology & Applied Microbiology
F. Bajerski, A. Buerger, B. Glasmacher, E. R. J. Keller, K. Mueller, K. Muehldorfer, M. Nagel, H. Ruedel, T. Mueller, J. Schenkel, J. Overmann
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Agriculture, Dairy & Animal Science
Karin Mueller, Susanne Eder, Ulrike Jakop, Juergen Schiller, Peter Mueller, Mohammad Bashawat
REPRODUCTION IN DOMESTIC ANIMALS
(2020)
Article
Biology
M. Bashawat, B. C. Braun, K. Mueller
Article
Reproductive Biology
Susanne Eder, Liam D. Bailey, Karin Mueller
Article
Reproductive Biology
M. Schulze, U. Jakop, F. Schroeter, C. Herrmann, C. Leiding, K. Mueller, M. Jung, G. A. Czirjak
Review
Agriculture, Dairy & Animal Science
M. Bashawat, B. Hensel, K. Mueller, M. Schulze
Summary: This review provides an overview of current techniques in cooled storage of semen from livestock animals such as camelids, goats, and sheep, addressing factors influencing semen handling and discussing methods to improve liquid-storage of cooled semen. Species-specific physiology and challenges are taken into consideration, with goat semen showing the greatest pregnancy rates after cooled liquid-storage for several days.
ANIMAL REPRODUCTION SCIENCE
(2021)
Article
Reproductive Biology
M. Schulze, F. Mohammadpour, F. Schroeter, U. Jakop, H. Hoenicke, T. Hasenfuss, H. Henne, J. Schoen, K. Mueller
Summary: Apart from traditional semen parameters, semen stress tests are useful tools in assessing the fertilizing capacity of sperm. Studies have shown that the correction of fertility parameters for female and male effects is crucial for the effectiveness of SSTs. Analysis of 260 ejaculates and 1521 insemination records revealed significant effects of factors such as sperm quantity, motility, and morphology on farrowing rate and number of total born and live born piglets.
Article
Biochemical Research Methods
Jorin Diemer, Jens Hahn, Bjoern Goldenbogen, Karin Mueller, Edda Klipp
Summary: Sperm migration and selection in the female genital tract play a crucial role in successful fertilization, with many underlying mechanisms remaining unknown. A computational model approximating the female reproductive tract shape and integrating interactions between sperm and their environment reveals which interactions could be most beneficial for fertilization. Predictions from the model will enhance assisted reproduction in humans, endangered species, and livestock.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Reproductive Biology
Pascal D. Kroh, Beate C. Braun, Fan Liu, Peter Mueller, Karin Mueller
Summary: The interaction between boar spermadhesin AWN and membranes is inconsistent. This study using various microscopy techniques and lipid-binding partner analysis, located AWN primarily in the equatorial segment (EQS) of boar sperm and identified an electrostatically driven binding mechanism. These findings provide new insights into the role of AWN in sperm-oocyte fusion.
BIOLOGY OF REPRODUCTION
(2022)
Article
Multidisciplinary Sciences
Ulrike Jakop, Karin Mueller, Peter Mueller, Stefanie Neuhauser, Isabel Callealta Rodriguez, Sonja Grunewald, Jurgen Schiller, Kathrin M. Engel
Summary: By comparing the phospholipid compositions of semen and blood from different species, it was found that the composition of blood phospholipids is similar across species, while significant differences exist in sperm and seminal fluid. The flexibility of sperm membrane is crucial for sperm motion and fertilization, but it also increases the risk of oxidation. Bull sperm, due to their high content of plasmalogens, are most susceptible to oxidation. While the protective capacity of human seminal fluid is well developed, it can be compromised by oxidative challenges related to lifestyle factors, leading to significant contamination with harmful lyso-phospholipids.
Article
Biotechnology & Applied Microbiology
Susanne Eder, Karin Mueller, Shuai Chen, Jennifer Schoen
Summary: Understanding the cellular and molecular mechanisms of feline reproduction is crucial for improving reproductive biotechnologies in endangered felids. This study successfully established a compartmentalized long-term culture system for feline oviduct epithelial cells, maintaining their differentiated state.
Article
Virology
Hannah Sabeth Sperber, Kathrin Sutter, Karin Mueller, Peter Mueller, Roland Schwarzer
Summary: Mammalian seminal plasma contains various bioactive components that play important roles in fertilization. The seminal plasma protein PDC-109 not only affects sperm function but also exhibits antimicrobial and antiviral activity, potentially limiting the sexual transmission of infectious diseases. Recent research has found that PDC-109 inhibits the membrane fusion activity of influenza virus particles and significantly impairs viral infections, including SARS-CoV-2. These findings suggest that PDC-109 has broad antiviral activity against multiple virus species and families.
Article
Biochemistry & Molecular Biology
Karin Mueller, Peter Mueller, Fan Lui, Pascal D. Kroh, Beate C. Braun
Summary: The major component of porcine seminal plasma, spermadhesin AQN-3, was found to selectively interact with negatively charged lipids. This interaction is likely mediated by factors such as electrostatic attractions. Further investigation is needed to determine if AQN-3, along with other sperm-associated seminal plasma proteins, acts as a decapacitation factor by targeting negative lipids with signaling or other functional roles in fertilization.
CHEMISTRY AND PHYSICS OF LIPIDS
(2023)