Article
Chemistry, Analytical
Xiaojun Liu, Yuanyuan Sun, Xinyi Lin, Xiaoyan Pan, Zhangjian Wu, Hongwei Gai
Summary: This paper presents a spectral imaging-based multiplex, homogenous immunoassay that was used to detect tumor biomarkers and successfully quantify model proteins in human plasma, demonstrating the accuracy and reliability of the approach.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Jingming Zhou, Zhanxiang Liu, Qingbao Yang, Wenjing Qian, Yumei Chen, Yanhua Qi, Aiping Wang
Summary: This study successfully developed a sensitive and accurate multiple fluorescence immunoassay for the simultaneous quantitative detection of ZEN and OTA. The method utilized multicolor QDs labeling, with IC50 values of 0.034 and 1.175 ng/mL, suitable for the simultaneous quantitative detection of various mycotoxins. The M-FLISA showed good consistency with HPLC results, confirming its reliability and efficiency in mycotoxin detection.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Food Science & Technology
Qingbao Yang, Yanhua Qi, Jingming Zhou, Hongliang Liu, Yankai Liu, Yumei Chen, Chao Liang, Aiping Wang
Summary: This study established a new method for detecting Zearalenone (ZEN) in corn. The method utilizes quantum dots and antibodies, and the detection is performed through immunochromatographic test strips. The experimental results showed that this method can rapidly and sensitively detect ZEN in corn.
FOOD ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Fei Ma, Ning-ning Zhao, Meng Liu, Qinfeng Xu, Chun-yang Zhang
Summary: This study reports a new single-molecule fluorescent biosensor for the simple and ultrasensitive detection of alkaline phosphatase (ALP). The biosensor enables efficient amplification and transduction of the target ALP signal through enzyme-free assembly and disassembly processes, significantly simplifying the experimental procedure and improving the assay accuracy.
ANALYTICAL CHEMISTRY
(2022)
Article
Agriculture, Dairy & Animal Science
Shuangyan Ding, Hailiang Hu, Xianglin Yue, Kaiwen Feng, Xiaoyu Gao, Qiuling Dong, Mingqi Yang, Ugur Tamer, Ganhui Huang, Jinsheng Zhang
Summary: A new detection method based on quantum dots was developed to detect Salmonella in milk, with a detection limit 10 times better than conventional sandwich ELISA. The biosensor showed good specificity for detecting Salmonella.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Cara E. Bradsher, Cayla D. Ontko, Alexandra C. Koziel, James R. McBride, Sandra J. Rosenthal
Summary: This research has found that ferroelectric behavior can be achieved in nanoparticles through ion exchange, with fluorescence retention achieved through CdS shelling before ion exchange, containing defects in the CdS shell rather than the fluorescent CdSe cores. The ferroelectric response remains constant with an increase in the number of CdS monolayers, while fluorescence retention also increases, reaching 99% at 8 monolayers, allowing for the addition of ferroelectric applications to the growing list of quantum dot applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Olga A. Goryacheva, Natalia Beloglazova, Irina Yu Goryacheva, Sarah De Saeger
Summary: Semiconductor quantum dots (QDs) are widely used as luminescent labels in food and medical analysis, and in combination with gold nanoparticles (GNs) as energy acceptors in Forster resonance energy transfer (FRET) pairs to develop highly sensitive biosensors. The developed homogeneous FRET-based immunoassay for detecting deoxynivalenol (DON) demonstrates the potential of fast and high-throughput mycotoxin control with a low limit of detection.
Article
Chemistry, Applied
Mei Hu, Xiaofei Hu, Guoqiang Wang, Yongxia Cheng, Xiaoling Yu, Xianqing Huang, Yu Li
Summary: A portable fluorescence immunosensor based on CdSe/CdS/ZnS quantum dots (QDs) was developed for the precise quantification of olaquindox (OLA). The QDs labeled anti-OLA antibody served as a bioprobe and greatly enhanced the sensitivity of the lateral flow test strip. The method achieved quantitative results within 8 minutes using a fluorescent strip scan reader, with a detection limit of 0.12 μg/kg, 2.7 times more sensitive than the conventional colloidal gold-based strips. Acceptable recovery rates of 85.0%-95.5% were obtained for spiked samples. This QDs-based strip immunoassay method is suitable for on-site detection and rapid initial screening of OLA in swine feedstuff, and has the potential for detecting other veterinary drugs to ensure food safety.
Article
Chemistry, Analytical
Yingyu Chen, Yonggang Hu, Bingjie Zheng, Yuqing Qin, Yuqiang Xiang, Aizhen Guo
Summary: In this study, a simple, sustainable, low-cost, and safe strategy for preparing fluorescence-encoded microbeads (FEBs) is proposed by assembling fluorescent proteins (FPs) onto magnetic microbeads (MBs) via chemical coupling. The FP-based FEBs have high stability and can tolerate the use of organic solution. The method enables multiplex detection of femtomolar ssDNA molecules and shows advantages such as high sensitivity, specificity, accuracy, repeatability, rapidity, and cost-effectiveness, making it suitable for various research fields.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jianfeng Yan, Quanbin Fu, Shikai Zhang, Xianbao Shi, Yuanhong Zhang, Juying Hou, Junling Duan, Shiyun Ai
Summary: An excellent dual-emission ratiometric fluorescent sensor for eight kinds of biogenic amines was established by mixing pH-responsive carbon dots and Rhodamine B. The sensor showed a rapid response (10 s), low limit of detection (0.733-3.943 μM) and a wide linear range (0-150 μM). A portable fluorescent filter paper was prepared by dispersing carbon dots and Rhodamine B in a solution, which showed an obvious color change in the presence of biogenic amine vapor from shrimp and mushroom. The sensor enables on-site and visual detection of biogenic amines in real food samples, making it suitable for potential application in food safety monitoring.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Biochemical Research Methods
Manivannan Madhu, Chien-Min Chao, Chen-Yi Ke, Ming-Mu Hsieh, Wei-Lung Tseng
Summary: A triple-readout probe has been developed for the colorimetric, fluorescent, and fluorescence-lifetime sensing of alkaline phosphatase (ALP) using silver nanoparticles (AgNPs) formed on molybdenum disulfide quantum dots (MoS2 QDs). The probe shows high sensitivity and selectivity for the detection of target proteins in clinical samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Peng Qu, Cheng-Jie Li, Jin Hu, Ge Gao, Peng Lin, Wei-Wei Zhao
Summary: Organicphotoelectrochemical transistor (OPECT) biosensing is a promising approach for biomolecular sensing. This work demonstrates the direct enzymatic biocatalytic precipitation (BCP) modulation on a flower-like Bi(2)S(3) photosensitive gate, achieving high-efficiency OPECT operation with high transconductance (g (m)). The developed OPECT aptasensor shows good analysis performance for prostate-specific antigen (PSA).
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Yifeng Ding, Wenjuan Zhu, Chenxi Huang, Yiming Zhang, Jia Wang, Xiaohong Wang
Summary: A novel approach to Salmonella detection using quantum dot labeled phage-encoded RBP 55 as a fluorescent nanoprobe is reported in this study. The method combines immunomagnetic separation and RBP 55-QDs to form a sandwich composite structure for the detection of Salmonella. The results show a good linear correlation between fluorescence values and Salmonella concentration, with a low detection limit of 2 CFU/mL within 2 h. The method has been successfully used to detect Salmonella in spiked food samples and could potentially be used for the simultaneous detection of multiple pathogens in the future using polychromatic QDs labeled with different phage-encoded RBPs.
Review
Biotechnology & Applied Microbiology
Hong Ruan, Li Zhou
Summary: The rapid industrialization has caused serious environmental problems and increased disease and healthcare issues. The development of simple and effective biosensors is crucial for disease prevention and treatment. Sulfur quantum dots, as a new fluorescent nanomaterial, offer great potential in the fields of bioimaging and biosensing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yonghong Song, Yueqiang Zhu, Kun Jiang, Xingyu Liu, Liang Dong, Dongdong Li, Sheng Chen, Hanye Xing, Xu Yan, Yang Lu, Xianzhu Yang, Junxia Wang, Yunjun Xu
Summary: Multifunctional magnet-fluorescent nanocomposites have wide applications in biomedical field. In this study, highly ferrimagnetic magnetic nanoparticles and ZnS/InP quantum dots were encapsulated in an amphiphilic block copolymer to obtain ferrimagnetic fluorescent micelles (FMFM) with uniform diameter and long-term stability. The FMFM showed excellent magnetic heating effect and magnetic resonance imaging capability, achieving improved magnetic hyperthermia therapy efficacy both in vitro and in vivo.
Article
Chemistry, Applied
Asifa Sarwat, Waqar Rauf, Saima Majeed, Marthe De Boevre, Sarah De Saeger, Mazhar Iqbal
Summary: Mycotoxins pose a threat to animal and human health and contribute significantly to economic losses in the poultry sector. A study conducted in Punjab, Pakistan found a co-occurrence of multiple mycotoxins and contamination in poultry feed samples, with many samples exceeding the European Commission's limits.
FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE
(2022)
Article
Chemistry, Applied
Abebe Ayelign, Taddese Alemu, Sarah De Saeger
Summary: A method based on HACCP-based SOP has been validated in cash crop-producing communities in Southern Ethiopia. The majority of mothers understand the importance of complementary feeding and are motivated to implement the method. However, some mothers lack awareness of the health risks of aflatoxins. The study found contamination of CFs with aflatoxins.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2022)
Article
Food Science & Technology
V Kagot, M. De Boevre, S. De Saeger, A. Moretti, M. Mwamuye, S. Okoth
Summary: This study evaluated the incidence, regional distribution and toxigenic potential of Aspergillus and Fusarium species occurring in maize kernels from different agro-ecological zones in Kenya, providing useful data for targeted mycotoxin mitigation strategies in the future.
WORLD MYCOTOXIN JOURNAL
(2022)
Article
Food Science & Technology
Waldo Deroo, Larissa De Troyer, Frederic Dumoulin, Sarah De Saeger, Marthe De Boevre, Steven Vandenabeele, Leen De Gelder, Kris Audenaert
Summary: This work presents an alternative workflow for discovering bacterial biocontrol agents against Fusarium head blight (FHB) in wheat. By enriching and isolating bacterial strains through multiple cycles, researchers found that some strains were able to reduce infection symptoms and fungal biomass. However, the analysis also showed that some strains did not reduce mycotoxin levels significantly. The isolated bacterial strains have the potential to reduce the infection pressure of F. graminearum on wheat spikes.
Article
Food Science & Technology
N. N. Truong, K. Tesfamariam, L. Visintin, T. Goessens, S. De Saeger, C. Lachat, M. De Boevre
Summary: Mycotoxin contamination poses a global challenge to food safety and population health, leading to a range of adverse effects on human health. The accumulative effects of multiple mycotoxin exposures on health outcomes are not well understood. Various statistical approaches, such as generalised linear regressions in conjunction with regularisation methods and principal component analysis, have been used to assess mycotoxin exposure-health outcome associations, but they have certain limitations. Recently, alternative approaches such as weighted quantile sum regression and Bayesian kernel machine regression have been introduced to address the issues of high dimensionality and highly-correlated data in this context.
WORLD MYCOTOXIN JOURNAL
(2023)
Review
Chemistry, Analytical
Alexandra I. Arzhanukhina, Nadezhda S. Komova, Anton M. Pavlov, Alexey A. Serdobintsev, Tatiana Yu. Rusanova, Irina Yu. Goryacheva
Summary: Surface-enhanced Raman spectroscopy (SERS) is a powerful and up-to-date method in analytical chemistry, known for its high sensitivity and fingerprint recognition capabilities. In the medical field, SERS has been actively developed for label-free analysis of biologically important substances and potential on-site detection of toxins and food safety. Plasmonic nanostructures are essential for obtaining SERS signals, and electrospun nanofibers are a promising substrate due to their various advantages. This review summarizes the methods of incorporating plasmonic nanostructures into electrospun nanofiber substrates and provides detailed insights into the analytical applications of SERS-active electrospun nanofibers with embedded nanostructures for biologically significant molecules. Furthermore, the future prospects of these substrates in bioanalysis are discussed as the most promising area in analytical chemistry.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
P. A. Demina, O. A. Sindeeva, A. M. Abramova, M. S. Saveleva, G. B. Sukhorukov, I. Y. Goryacheva
Summary: This article investigates the performance of fluorescent photoconvertible polymer markers under different laser conditions and successfully achieves cell labeling. The authors describe the performance characteristics of fluorescent polymer capsules as an aqueous suspension and as a single capsule, and demonstrate the potential of these fluorescent markers for long-term live cell labeling.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Chemistry, Applied
Ye Tian, Mohamed F. Abdallah, Marthe De Boevre, Cheng Wang, Kris Audenaert, Sarah De Saeger, Aibo Wu
Summary: This study utilized an integrated workflow of mycotoxin analysis and non-targeted metabolomics to reveal the metabolic changes of Alternaria pathogens and the effects of Trichoderma strains on mycotoxin metabolism. The results showed that Trichoderma strains significantly influenced the whole metabolome and mycotoxin production of Alternaria pathogens, and one Trichoderma strain could metabolize alternariol into its hydroxylated form. These differential metabolites revealed fungal physiological alterations under various confrontation conditions.
Article
Food Science & Technology
Addisalem Mesfin, Carl Lachat, Seifu Hagos Gebreyesus, Meselech Roro, Kokeb Tesfamariam, Tefera Belachew, Marthe De Boevre, Sarah De Saeger
Summary: This study assessed the presence of multiple mycotoxins in breast milk and examined the relationship between total fumonisins and pre/post-harvest and dietary practices. The findings showed that the overall exposure to mycotoxins in breast milk was low, but fumonisins contamination was still present. No association was found between total fumonisins and pre/post-harvest and dietary practices.
Article
Pharmacology & Pharmacy
Orphelie Lootens, Marthe De Boevre, Jia Ning, Elke Gasthuys, Jan Van Bocxlaer, Sarah De Saeger, An Vermeulen
Summary: This article investigates the pharmacokinetic characteristics of aflatoxin B1 (AFB1) and finds that certain drugs can affect the metabolism of AFB1, while AFB1 has little impact on the metabolism of drugs.
Article
Chemistry, Analytical
Svetlana A. Mescheryakova, Ivan S. Matlakhov, Pavel D. Strokin, Daniil D. Drozd, Irina Yu. Goryacheva, Olga A. Goryacheva
Summary: Doxorubicin (DOX) is a commonly used anti-tumor drug in chemotherapy, but it has high cardio-, neuro- and cytotoxicity. Therefore, continuous monitoring of DOX concentrations in biofluids and tissues is important. This study demonstrates the capabilities of analytical nanosensors based on the quenching of fluorescence of alloyed CdZnSeS/ZnS quantum dots (QDs) for operative DOX detection. Optimized turn-off fluorescence nanosensors were developed for direct DOX determination in undiluted human plasma.
Article
Chemistry, Physical
Natalia E. Markina, Irina Yu. Goryacheva, Alexey V. Markin
Summary: The paper discusses the use of CD-modified AgNPs for the improvement of MTX determination through SERS. The CD-modified AgNPs increased the SERS signal of MTX in neutral media, simplifying the analysis and reducing the influence of endogenous components. The assay showed satisfactory precision, accuracy, and limit of detection for MTX determination in real human urine samples.
COLLOIDS AND INTERFACES
(2023)
Article
Nutrition & Dietetics
Yuri Bastos-Moreira, Lionel Ouedraogo, Marthe De Boevre, Alemayehu Argaw, Brenda de Kok, Giles T. Hanley-Cook, Lishi Deng, Moctar Ouedraogo, Anderson Compaore, Kokeb Tesfamariam, Rasmane Ganaba, Lieven Huybregts, Laeticia Celine Toe, Carl Lachat, Patrick Kolsteren, Sarah De Saeger, Trenton Dailey-Chwalibog
Summary: This study aims to evaluate the physiological effect of multi-micronutrient-fortified BEP supplementation on pregnant and lactating women and their infants. Biological specimens will be collected and multi-omics profiles and biomonitoring markers will be used to assess the effects of BEP supplementation on maternal health, birth outcomes, and infant growth.
Review
Food Science & Technology
Xiangrong Chen, Mohamed F. Abdallah, Sofie Landschoot, Kris Audenaert, Sarah De Saeger, Xiangfeng Chen, Andreja Rajkovic
Summary: This review provides an updated overview of the (co-)occurrence and (co-)contamination of A. flavus and F. verticillioides and their mycotoxins in maize. The gathered data predict an increase in their occurrence and virulence worldwide, especially in European cold climate countries. While antagonistic interactions were observed between these fungi, the co-contamination of AFB1 and FB1 has significantly risen in Europe.
Review
Food Science & Technology
Octavian Augustin Mihalache, Marthe De Boevre, Luca Dellafiora, Sarah De Saeger, Antonio Moretti, Laetitia Pinson-Gadais, Nadia Ponts, Florence Richard-Forget, Antonia Susca, Chiara Dall'Asta
Summary: This systematic review aims to update the knowledge on the occurrence and co-occurrence of non-regulated mycotoxins, while also providing an overview of current regulations. The study found 15 non-regulated mycotoxins in 19 food categories worldwide, with 38 different combinations of these mycotoxins. The authors emphasize the importance of continuous monitoring, scientific exchange, and generation of high-quality data to effectively address concerns related to non-regulated mycotoxin toxicity. Additionally, obtaining more data concerning the co-occurrence of regulated and non-regulated mycotoxins could support multiple chemical risk assessment methodologies.